Objective To investigate whether SUMO-1 enhances the apoptosis induced by wild-type p53 plasmid transfection in HepG2 cells. Methods The HepG2 cells were transfected respectively or simultaneouly with the following expressional plasmids as pcDNA3-wtp53(pwtp53,including human wild-type p53 gene),pCMV-HDM1B(pMDM2,including HDM2 gene, homologous gene as murine double minute gene 2),pcDNA3-His6-SUMO-1(pSUMO-1 ,including small ubiquitin-like modifier-1 gene)and plasmid pcDNA3.The proteins expressed in cells were detected by means of Western blotting and the apoptosis rates of cells were measured by flow cytometry. Results The protein bands of p53 and MDM2 could be seen in cells transfected with pwtp53 and pMDM2. Meanwhile,the relative larger molecular weight bands were also seen in cells transfected with pSUMO-1 which represented the p53 and MDM2 protein modification by SUMO-1. Merely the trace of p53 protein was detected in cells not transfected with any plasmid or only transfected with empty plasmid and pSUMO-1. In cells transfected with pwtp53 and pwtp53+pSUMO-1,the apoptosis rates were (16.79?1.62)% and (18.15?1.36)%. When transfected with pwtp53+pMDM2,the rate decreased to (5.17?1.23)%. The apoptosis rate would come up again to (14.06?1.84)% after transfected with pwtp53+pMDM2+pSUMO-1 and the difference of rates were significant compared to the cells transfected with pwtp53+pMDM2 (P

Objective To study China's policy of health human resources accommodated with the socio-economic development and medical demands. Methods The literature consultation method was used to analyze the status and experience in health human resources development in China since the reform and opening-up to the outside world over the past 30 years. Results Health human resources are the main motive power and decisive factor in the development of China's health work. Condition It is imperative to establish a mechanism to enhance and bring the initiative of health professionals into fall play, and set up a unified evaluation system consisting of responsibility, right and benefit, and a system of classified management of health human resources.

Objective: To observe the effects of panaxtriol saponin (PTS) on CK-MB, cTnI, IL-6, TNF-α, and ultrastructure against myocardial ischemia-reperfusion injury (MIRI) in rats. Methods: Wistar rats were randomly divided into six groups: Sham, MIRI model, positive control, and 22.5, 45, and 90 mg/kg PTS groups. Animal MIRI model was made by ligating the left anterior descending coronary artery. Drug was given for seven consecutive days before the operation by ip injection. Rats were sacrificed after 30 min ischemia and 24 h reperfusion. CK-MB, cTnI, IL-6, and TNF-α in serum were measured and the changes of myocardial ultrastructure were observed. Real time PCR was used to evaluate change of NRF2 and HO-1 mRNA level in myocardial tissue. Results: Compared with model, PTS 45 and 90 mg/kg reduced the CK-MB, cTnI, IL-6, and TNF-α in serum and improve the myocardium ultrastructure. Nrf2 and HO-1 mRNA levels in PTS treatment group were higher than those in MIRI model group. Conclusion: PTS plays a crucial role in cardioprotection against the ischemia and reperfusion injury in rats. The protective mechanism suggests that PTS could stimulate NRF2/HO-1 expression in myocardial tissue and then inhibit myocardial inflammation.

Traditional Chinese medicine is the treasure of China, which has played an indelible role in the process of fighting against diseases. Under the background of times of building Healthy China, how to play the unique advantages of traditional Chinese medicine in the disease prevention and treatment is the focus of attention. Traditional Chinese medicine has the characteristics of "multi-component, multi-target, and overall regulation", and it's not enough to reveal its internal essence only by the way of "local concept", so the research thinking of "overall concept"is imperative. System biology, a research technology, which understands the organism from a holistic perspective and coincides with the way of "overall concept"of traditional Chinese medicine. Therefore, the research strategy of traditional Chinese medicine based on system biology with highly feasible. In this paper, the application of system biology technologies, such as genomics, transcriptome, proteomics, metabonomics, microbiomics, omics combination and network pharmacology technology, in the research of ingredients, pharmacology and toxicology, identification, cultivation and genetic breeding of traditional Chinese medicine was reviewed, in order to provide reference for the further application of system biology strategy in the research of traditional Chinese medicine, based on the idea of "overall concept".

OBJECTIVE: To investigate the protective mechanism of red ginseng extract(RG) on aging mice induced by D-galactose and the correlation between tumor necrosis factor-α (TNF-α)glutathione peroxidase 1 (GPX1), glutathione peroxidase 2 (GPX2), interleukin-1β (IL-1β), interleukin-6 (IL-6) and interleukin-10 (IL-10). METHODS: Aging mice models were caused by D-galactose and randomly divided into control group, model group, RG low, medium and high dose (100, 200, 400 mg•kg-1) groups. The preparation of model and drug delivery, after 42 d, the mice were killed and determination of spleen index; the activity of superoxide dismutase (SOD) and malondialdehyde (MDA) in serum and brain tissue were measured. In serum, the levels of acetylcholinesterase (AChE), catalase (CAT), glutathione peroxidase (GSH-PX), inducible nitric oxide synthase (i-NOS) were measured. Enzyme-linked immunosorbent assay (ELISA) was used to test the levels of cyclooxygenase-2 (COX-2), TNF-α, IL-1β and IL-6 in brain tissue. Real time quantitative reverse transcription polymerase chain reaction (RT-PCR) was used to detect the correlation of TNF-α, GPX1, GPX2, IL-1β, IL-6 and IL-10 gene mRNA expression in brain tissue. RESULTS: The RG can enhance the spleen index, increase the activity of SOD in serum and brain tissue and AChE, CAT and GSH-PX in serum, decrease the content of MDA in serum and brain tissue, and decrease the content of iNOS in serum and the content of COX-2, TNF-α, IL-1β and IL-6 in brain tissue. At the same time, it significantly inhibited the mRNA expression of TNF-α, IL-1β and IL-6 in the brain tissue of mice, and increased the expression of GPX1, GPX2 and IL-10 mRNA in the brain tissue, and had statistical significance. CONCLUSION: Concentrated solution of red ginseng extract could significantly exhibit anti-aging effects, its mechanism may be related to the TNF-α, GPX1, GPX2, IL-1β, IL-6 and IL-10 expression in aging, enhancing immune function, and removing free radicals and inflammatory factor.

OBJECTIVE: To establish an HPLC method for determining four kinds of rare saponins, ie, 20(S)-Rg3, 20(R)-Rg3, Rk1, and Rg5 in black ginseng. METHODS: COSMOSIL C18-PAQ (4.6 mm×250 mm, 5 μm) column was used and temperature was maintained at 30℃. Gradient elution was conducted using mobile phase consisting of acetonitrile and water at a flow rate of 1.0 mL·min-1. The detection wavelength was set at 203 nm. The injected sample volume was 10 μL. RESULTS: Good resolution was achieved for the four rare saponins in the ranges of 0.051-0.256 (r=0.999 9), 0.009-0.280(r=0.999 7), 0.051-0.303(r=0.999 9) and 0.093-0.279 mg·mL-1(r=0.999 9) for 20(S)-Rg3, 20(R)-Rg3, Rk1, and Rg5, respectively. The corresponding average recovery rates were 103.9%, 99.2%, 97.0%, and 100.6%, and the standard deviations were 0.71%, 0.73%, 1.97%, and 0.57%, respectively. CONCLUSION: The method is accurate, simple, reliable and reproducible for the determination of saponins in black ginseng. The determination result can be used as a reference for the rational medication, quality control, and further study of black ginseng.

OBJECTIVE: To study the anti-fatigue effect of dihydromyricetin on mice and its anti-fatigue mechanism. METHODS: Forty male mice were randomly divided into blank control group and dihydromyricetin dose groups (40, 20, 10 mg·kg-1), with continual intragastric administration for 28 d. Through the weight loading swimming experiment of mice, the swimming time of mice were recorded. On the twenty-nine day, mice swam 90 min without loading, and then the activities of BUN, BLA in the serum of mice and biochemical indicators of SOD, GSH-Px, MDA and hepatic glycogen etc were respectively determined. The expression of mRNA of PGC-1α and PPARα were detected by real-time PCR. RESULTS: Comparing with the blank control group, dihydromyricetin dose group can prolong the loaded-swimming time of mice, increase the reserve of liver glycogen in the body, reduce levels of BLA, BUN and MDA, and significantly increased the activities of SOD and GSH-Px. RT-PCR RESULTS showed that the expression levels of PGC-1α and PPARα mRNA in skeletal muscle of mice in the dihydromyricetin dose group were significantly increased, and all had statistical significance. CONCLUSION: Dihydromyricetin can accelerate clearance of free radical, enhance mice antioxidant capacity and improve exercise ability in fatigued mice thus play the anti-fatigue role, exercise-induced fatigue is correlated with PGC-1α.

Aged ; Diskectomy, Percutaneous ; Endoscopy ; Humans ; Intervertebral Disc Displacement/surgery* ; Lumbar Vertebrae/surgery* ; Spinal Stenosis/surgery* ; Treatment Outcome

BACKGROUNDPercutaneous coronary intervention (PCI) causes endothelial damage, resulting in an inflammatory response with elevation of markers such as high-sensitive C-reactive protein (hs-CRP) and vascular cell adhesion molecule-1 (VCAM-1), which are associated with restenosis after PCI. Evidence suggests that microRNA-126 (miR-126) plays an important role in vascular inflammation, but its correlation with PCI-mediated inflammation has not been investigated. In this study, we investigated the effect of PCI on circulating miR-126 and inflammation markers such as hs-CRP and VCAM-1.

METHODSWe enrolled 130 patients with coronary artery disease (CAD) in the Second Hospital of Jilin University from October 2015 to December 2015. Among them, 82 patients with CAD, defined as at least one major epicardial vessel with >70% stenosis who planned to undergo PCI, were divided into acute coronary syndrome (ACS) group (46 patients) and stable angina (SA) group (36 patients). Forty-eight patients confirmed by coronary angiography without PCI were used as controls. The plasmas of all patients were collected prior to PCI and at 30 min, 24 h, and 72 h after PCI. The plasma VCAM-1 and hs-CRP were detected by enzyme-linked immunosorbent assay, and the miR-126 was evaluated by quantitative reverse transcription-polymerase chain reaction.

RESULTSPlasma concentrations of hs-CRP and VCAM-1 in patients with either ACS (n = 46) or SA (n = 36) were significantly higher than in controls (n = 48) (P < 0.01) prior to PCI, and increased further at 24 h and 72 h after PCI, compared with prior PCI. Moreover, VCAM-1 was positively correlated with balloon time and pressure. In contrast, the plasma concentration of miR-126 was significantly lower in patients with CAD than in controls, and further decreased with time post-PCI. A negative correlation was observed between miR-126 and hs-CRP and VCAM-1 at 72 h after PCI.

CONCLUSIONThere was a negative correlation of miR-126 with the PCI-induced markers of inflammation such as hs-CRP and VCAM-1.

Acute Coronary Syndrome ; blood ; Angina, Stable ; blood ; C-Reactive Protein ; metabolism ; Coronary Angiography ; Coronary Artery Disease ; blood ; surgery ; Enzyme-Linked Immunosorbent Assay ; Female ; Humans ; Male ; MicroRNAs ; blood ; Middle Aged ; Percutaneous Coronary Intervention ; Reverse Transcriptase Polymerase Chain Reaction ; Vascular Cell Adhesion Molecule-1 ; blood

Objective: To observe the curative effect of non-surgical treatment under periodontal endoscope in the patients with moderate-to-severe periodontal pockets of multirooted teeth after scaling and root planning (SRP), and to expound the clinical significance of periodontal endoscope in assisting periodontal non-surgical treatment. Methods: After SRP, there were still more than 127 multirooted teeth with moderate-to-severe periodontal pockets in 24 patients with periodontitis. The above multirooted teeth received non-surgical treatment under periodontal endoscope, the control of periodontal risk factors was evaluated by the periodontal risk assessment (PRA) model; the probing depth (PD), bleeding on probing (BOP), attachment loss (AL), calculus residual rate of the patients after treatment were detected by Florida Probe system; the percentage of furcation defect was detected. Results: After 3 months of periodontal endoscope-assisted periodontal non-surgical treatment, the risk factors of the patients after treatment were reduced and the patients had good prognosis. The PD value and percentage of BOP positive sites of the teeth of the patients after treatment were significantly decreased compared with before treatment (P< 0. 01); the PD value of the near-distal median point was obviously decreased compared with the PD value of the central site (P< 0. 01); the positive rate of calculus was significantly decreased (P<0. 01); there was no significant difference in the AL (P>0. 05). The percentage of teeth with Class I furcation involvement among the multirooted teeth after treatment was significantly lower than before treatment (P<0. 05), but the percentage of teeth with Class II and above furcation involvement had no significantly difference between before and after treatment (P> 0. 05). Conclusion: The effect of periodontal endoscope-assisted non-surgical treatment for the multirooted teeth with residual moderate-to-sever periodontal pockets after SRP, and it can avoid periodontal operation to some extent.