Objective To discuss the clinical value of 64-slice CT angiography in the diagnosis of coronary artery stenosis.Methods 60 patients with suspected coronary artery disease were examined by CAG and 64SCT,then we contrasted the results,and diagnostic value of 64SCT in coronary artery stenosis was tested by CAG as the gold standard.Results The number of coronary section could clearly show in 64SCT(458) were significantly more than in the CAG(504)(x2 =20.1318,P＜0.01) ;but there was no significant difference in the diagnosis rates of coronary heart disease between the two methods (P ＞0.05) ;the sensitivity of 64SCT in coronary artery disease was 93.4％,specificity 96.0％,positive predictive value of 88.0％,and negative predictive value of 97.9％.Conclusion Compared with CAG deficiencies in the diagnosis of coronary artery stenosis,64SCT has higher value.,and it has high sensitivity,specificity,positive and negative predictive value in the diagnosis of coronary artery stenosis,and which is worthy of early screening for the disease.

Seventeen monoclonal antibodies specific for P.falciparum were tested for their ability 10 inhibit the multiplication of a partially synchronized culture of P. falciparum by parasite counts. Seven of these antibodies, ranging from culture supernatant and ascitic fluid to purified IgG, were shown to have a dose-depsndent inhibitory effect on parasite growth in vitro. At a final concentration of 0.6 mg/ml, the inhibitory capacity of purified IgG was above 90% over a 3-day culture period, greater than that of the relevant polyclonal antibodies. These monoclonal antibodies caused agglutination of free merozoites, multiple invasion of merozoites within a single erythrocyte, and/or degeneration of schi-zonts, suggesting that there may be several acting points of the inhibitory monoclonal antibodies. However, the mechanisms of action of the inhibitory antibodies varied with different monoclonal antibodies.

0.05). Monoclonal antibodies with spot-or pin-like fluorescence and honeycomb-like bright fluorescence on thc cdge of the mature parasite, recognizing surface antigens of free merozoite and segmenter, exhibited cxcellent protective activities.

The definition of environmental endocine disruptors,varieties of chemicals possible to produce endocrine disruptive effects,their possible mechanisms and adverse effects on organisms were briefly introduced in this paper.It suggested that there were many varieties of endocrine disrupting chemical existing in the human environment.The reproductive disorders reported up to date in animal maybe in human,included reduced fertility,reduced hatchability,redcued viability of offspring,impaired hormone secretion or activity and modified reproductive anatomy.Further study should be conducted and relative preventive measures should be adopted.

Objective To explore the developmental toxicity of tributyltin chloride and the effects on sex hormone in female offspring rats through maternal gestation exposure. Methods Pregnant Wistar rats were randomly divided into four groups, 4 in each group. They were treated with TBTC by gavage at the dose of 0, 1, 2.5 and 5 mg/kg bw respectively from days 12-20 of gestation. 10 female offspring rats were randomly selected from each group and killed on postnatal day 70. The liver, kidney, uterus and ovary were weighed and the organ indexes were calculated. Pathological examination for liver, kidney, uterus and ovary were performed. Follicle stimulating hormone(FSH), luteotropic hormone(LH), testosterone(T) and estradiol(E2) in serum was determined by radioimmunity method. Results Increase of body weight in 2.5, 5 mg/kg bw groups significantly decreased(P

Objective To study the effects of di-butyltin dilaurate (DBTD) exposure on pregnancy outcome in Wistar rat and evaluate it's effect on sexual development of fetuses. Methods Timed pregnancy rats were treated with corn oil or DBTD (10, 20, 30 mg/kg body weight) from days 12-19 of gestation. On gestational day (GD) 20, dams were sacrificed to investigate the pregnancy outcome. Results There was a downtrend in weight of dams on GD20 as the DBTD exposure dose increased. The weight of dam's utero significantly decreased in 30 mg/kg group. A significant decrease in fetal weights was observed in all DBTD groups, and fetal sizes in 20, 30 mg/kg groups. Exposure to DBTD from GD 12-19 resulted in a distinct increase in normalized anogenital distances in female fetuses, but no effects were seen in male ones. DBTD exposure did not result in external malformations, however, delayed ossification of fetal phalanges was observed in all DBTD treated groups. Dead fetuses and absorbed embryos were observed in 20, 30 mg/kg DBTD groups. Conclusion The results of the present paper show that DBTD exposure has some adverse effects on fetal development and may exert a masculinizing effect on female fetuses.

Objective To develop a new method to screen the potential microcystin-producing cyanobacteria in natural waters. Methods Cyanobacteria were cultured and waters samples were analyzed by polymerase chain reaction with special primers for gene of the operon mcyD which encodes a microcystin synthetase combining conserved gene 16S rRNA of alga, subsequently, whose microcystins were detected by ELISA. Results There was a unique amplified product of approximately 870 bp in microcystin-producing blue-green alga and water samples, but no target band in non-toxic strains and samples. Conclusion It is feasible to use the molecular biological method used mcyD as the microcystin molecular biomarker to screen microcystin-producing cyanobacteria in natural water.

Objective To investigate clinical and pathological features of autoimmune cholangitis (AIC) in antimitochondrial antibody (AMA) positive and negative patients.Method According to AMA presence or absence,37 primary biliary cirrhosis (PBC) patients were assigned to AMA positive group and AIC group.Clinical and pathological differences between two groups were compared.Results No differences were found in the level of liver function,liver cirrhosis,frequency of cholestasis and bile duct lesions between two groups.However,lower serum level of alkaline phosphatase,severer hepatocellular damage in pathology and higher AIH score were found in AIC group.Therefore the frequency accompanied by Sjgren′s syndrome (SS) in AIC group was significant higher.Conclusion AIC group is companied by slighter cholestasis and severer damage of hepatocytes indicating more autoimmune findings of AIC patients.AIC may have some correlation with SS in etiology and pathogenesis.

0.05). HRF couldn′t be detected in 28 ankylosing spondylitis patients. The correlation was found between the serum levels of HRF and CRP in active RA patients (r=0.331,P

Aim To explore the role of endoplasmic reticulum stress (EPR) in high fatty acid induced injury in cardiomyocytes.Methods Cardiomyocytes were exposed to different concentrations of palmitic acid (0, 0.1, 0.2, 0.4 mmol·L-1) for 24 h and different time points of high palmitic acid (0.2 mmol·L-1) (12, 24, 48 h).Cell viability was measured by CCK8, and the protein expressions of EPR such as GRP78, CHOP, PERKphos, IRE1phos, ATF6 and apoptosis related proteins such as Bcl-2 and Bax were determined by Western blot.Results When the cells were stimulated with palmitic acid (0.1~0.4 mmol·L-1) for 24 h, the cell proliferation rates of 0.2 and 0.4 mmol·L-1 groups were significantly decreased.Cardiomyocytes exposured to high palmitic acid (0.2 mmol·L-1) for 24 h showed an increase in theexpression of EPR related proteins (GRP78, CHOP, PERKphos, IRE1phos and ATF6) and Bax(P<0.05), while Bcl-2 expression was significantly reduced.Pretreatmented with EPR inhibitor pravastatin(10 mol·L-1) significantly increased high palmitic acid induced Bcl-2 expression (P<0.05) and significantly decreased high palmitic acid induced Bax expression (P<0.05).Conclusion Activated EPR might play an important role in treatment of high fatty acid induced myocardial injury.