The truncated binary exponential back-off algorithm is one of the most effective methods applied in collision resolution process of random multi-access channel. In this study, two new strategies are presented to improve the capability of the truncated binary exponential back-off algorithm. In the new strategies, the sizes of the initial window size or the operating window sizes are adjusted dynamically, which always bring a significant improvement for the self-adaptability of the original algorithm. A series of experiments are simulated and the results verify that the new strategies can make the implementation more stable and effective than the original algorithm.

Objective: To investigate the impact of stent implantation on cognitive function in patients with mild-to-moderate cognitive dysfunction associated with severe stenosis of unilateral middle cerebral artery (MCA) M1 segment. Methods: According to whether stent implantation, 28 patients with mild-to-moderate cognitive dysfunction associated with unilateral MCA M1 severe stenosis were divided into treatment group (n=13) and control group (n=15). The examinations of mini-mental state examination (MMSE) score, Montreal cognitive assessment (MoCA) score and the event-related potential P300 were performed on all patients in treatment group 1 week before stent implantation and 6, 12 months after implantation,as well as in control group during the same period. Totally 5 patients in treatment group underwent CT perfusion imaging (CTP) 1 week before stent implantation and 1 month after implantation. Cerebral blood flow (CBF) and cerebral blood volume (CBV) were measured and the ratios of CBF and CBV between the affected side and contralateral side (rCBF and rCBV) were calculated. Meanwhile, mean transit time (MTT), peak time (TTP) and the differences of between the two sides (dMTT and dTTP) were obtained, and then statistical analysis was performed. Results: There was no significant difference of MMSE and MoCA scores, nor the latency and amplitude of P300 between the two groups 1 week before stent implantation (all P>0.05). However, 6 and 12 months after implantation and the corresponding period, the differences were statistically significant between the two groups (all P<0.05). In treatment group, the differences of the scores of MMSE and MoCA were statistically significant 1 week before stent implantation, 6 and 12 months after implantation (all P<0.05). The pairwise comparison showed that the scores of MMSE and MoCA 12 months after stent implantation were higher than those 1 week before implantation (both P<0.05), and the score of MoCA 6 months after implantation was higher than that 1 week before implantation (P=0.010). There was no significant difference of the latency and amplitude of P300 between the two groups 1 week before stent implantation (both P>0.05). The latencies in treatment group were shorter than those in control group 6 and 12 months after stent implantation (both P<0.01), while the amplitude were higher than those in control group (both P<0.05). In treatment group, the latency and amplitude of P300 were statistically significant 1 week before stent implantation, 6 and 12 months after implantation (all P<0.05). The pairwise comparison showed that the latencies of 6 and 12 months after stent implantation were shortened (both P<0.01), while the amplitude increased compared with those on 1 week before implantation (both P<0.05). Compared with 6 months after stent implantation, the latency on 12 months after implantation was shortened (P=0.010). In treatment group, no matter whether there were risk factors or not, no significant difference of the scores of MMSE nor MoCA among 1 week before stent implantation, 6 and 12 months after implantation (all P>0.05). Of 5 patients underwent CTP examination in treatment group, rCBF 1 month after stent implantation was higher than that 1 week before stent implantation (P<0.01), while rCBV, dMTT and dTTP were lower than those 1 week before implantation (all P<0.05). Conclusion: Stent implantation can improve cognitive function in patients with severe stenosis of MCA M1 segment through increasing blood flow of cerebral artery. Cognitive function changes can be detected earlier by latency of P300 than by MMSE score.

Aim To investigate the effect of dipfluzine on hERG potassium currents expressed heterologously in xenopus oocytes.Methods Using Xenopus oocytes expression system,the current amplitude and kinetic characteristics of hERG were measured with the two electrode voltage-clamp technique before and after dipfluzine application.Results Dipfluzine(8 nmol?L-1~5 ?mol?L-1)concentration-dependently inhibited hERG currents;the concentration for half maximal inhibition(IC50)was 98.0 nmol?L-1.Dipfluzine-induced inhibition of hERG currents was voltage dependent at membrane potentials between-10 and 40 mV.Dipfluzine at 1 ?mol?L-1 didn't statistically shifted V1/2 of hERG currents activation.Dipfluzine at 1 ?mol?L-1 significantly decreased the activating time constants and the deactivating time constants,and enhanced hERG currents activation and deactivation.Conclusion Dipfluzine concentration-dependently inhibits hERG currents and modifies kinetic characteristics of hERG activation and deactivation,which may be correlated with its antiarrhythmic effect.

Objective To clarify the function of LIM and SH3 domain protein-1 (LASP1) and ferritin in rhBMP2-induced osteogenic differentiation of beagle bone marrow mesenchymal stem cells (BMSCs).Methods After BMSCs from 3-18-month-old C57BL/6J mice were cultured adherently for 24 hours,they were subjected to osteogenic differentiation for 7,14 and 21 days in 3 groups.BMP2 (100 μg/L) and osteogenic differentiation medium was added in the experimental group,only osteogenic differentiation medium was added in the control group,and nothing was added in the blank group.Osteoblast differentiation was determined by examining marker genes (Runx2,OSX,OCN and OPN) using qRT-PCR.The protein expression of both LASP1 and ferritin was investigated using western blotting.After LASP1 and ferritin were silenced in the cells in the experimental group after transfection of shRNA to target LASP1(m),rhBMP2-induced osteogenesis was repeated to verify the roles of LASP1 and ferritin in osteoblast differentiation.Results The qRT-PCR showed successful osteoblast differentiation in the experimental group.Western blotting verified significant down-regulation of LASP1 and up-regulation of ferritin in the experimental group.After the LASP1 gene was silenced,the expression levels of osteoblast differentiation marker genes in the experimental group were higher than those in the control group.Conclusions rhBMP2 can induce mouse BMSCs to differentiate into osteoblasts in a significant manner.Combined with our preliminary research,the present study may confirm that LASP1 and ferritin,which play an important role in regulating cytoskeleton activity and iron metabolism,are critical in the osteogenic differentiation of mouse BMSCs induced by rhBMP2.

Objective To explore the clinical technical points of the treatment of soft tissue defect of the foot and ankle with the supercharged peroneal artery perforator propeller flap,and to provide theoretical support by anatomical observation.Methods From January,2010 to February,2018,a total of 10 patients with soft tissue defect of foot and ankle were treated with supercharged peroneal artery perforator propeller flap.Cause of injury:trauma in 7 cases,wound ulcer in 1 case,and poor healing of the calcaneus incision in 2 cases.Defect site:5 cases of heel,2 cases of medial and lateral malleolus,and 3 cases of dorsum and sole.The size of flap ranged from 6.0 cm×3.0 cm to 16.0 cm×5.0 cm.All patients were followed-up at 1,3,6 months after operation,and the function recovery was judged by AOFAS Ankle Hindfood Scale at 3 months post-opertively.From November,2016 to May,2017,the anatomical basis and operative points of the supercharged peroneal artery perforator flap were summarized.Results All the 10 cases of supercharged peroneal artery perforator propeller flap survived.Two of them had local epidermal necrosis at the proximal end of the flap.After 1 to 2 weeks of dressing,they finally healed.The other 8 cases healed well.Anatomical studies showed that different planes of the supercharged peroneal artery perforator propeller flap can only reduce the compression of the double pedicles and reduce the distal necrosis rate of the flap by rotating in different rotation directions.Conclusion The supercharged peroneal artery perforator propeller flap can enhance the blood supply and venous return in the "big paddle" artery of the flap,preventing distal necrosis.

According to the requirement of the national assessment for achieving the infection control criteria, 42 villages (among them,25 villages belonged to the first stratum, and 17 villages belonged to the second stratum) in 14 counties from 5 provinces, including Hunnan, Hubei, Jiangxi, Anhui and Yunnan, were selected as sampling villages for the assessment.The results from the field assessment showed that 154 out of 9 067 people were found infected with Sckistosoma japonicum, with an average infection rate of 1.7% ranged from 0.31 % to 4.10% , and only Yongping Village from Weishan County and Tenglong Village from Eryuan County were not found any case. A total of 46 out of 3 323 head of cattle were infected with S. japonicum, with an average infection rate of 1.38% ranged from 0.26% to 3.79% , and no any infected individual detected in Nanling County. No outbreak occurred in those sampling villages. Therefore, it is indicated that the five sampling provinces have reached the national criteria on infection control of schistosomiasis.

Arabidopsis ; chemistry ; genetics ; metabolism ; Arabidopsis Proteins ; chemistry ; genetics ; metabolism ; Crystallography, X-Ray ; Intercellular Signaling Peptides and Proteins ; chemistry ; genetics ; metabolism ; Protein Conformation ; Protein-Serine-Threonine Kinases ; chemistry ; genetics ; metabolism

Cryoelectron Microscopy ; methods ; Crystallography, X-Ray ; Image Processing, Computer-Assisted ; methods ; Models, Molecular ; Molecular Conformation