Objective To evaluate the new colposcopy combined cytology thinprep pap test(TCT) in cervical screening in the diagnosis of disease. Methods 1 266 patients detected by gynecologic cervical cytology were conducted on colposcopy and liquid-based thin-layer cytology of the Joint Inspection. Results 1 266 cases in which seized 169 cases of abnormal cervical lesion detection rate of 13. 35%,abnormalities in cervical squamous intraepithelial lesions and 126 cases,accounting for 9.99%. The incidence of cervical intraepithelial lesions detected no difference between age groups was statistically significant(P＞0.05). Conclusion Thinprep cytology combined colposcopy cervical precancerous lesions was a reliable method for early diagnosis.

OBJECTIVE To investigate drug-resistance status of Klebsiella pneumoniae in county hospital and provide the reference for rational application of antibiotics.METHODS Drug-resistance was analyzed by K-B methods.The ESBLs producing strains were detected by disc confirmed test.RESULTS The detection rate of ESBLs producing K.pneumoniae was 34.7% in our hospital.Among 216 strains of K.pneumoniae,the susceptibility rates to imipenem and meropenem were all 100.0%.And the resistant rate to amikacin was 25.0%.The resistant rates to the other 9 antibiotics were all over 30.0%.CONCLUSIONS Drug-resistance status of K.pneumoniae is very serious in county hospital.We should think highly of monitoring and controlling drug-resistance of clinical strains in order to prevent breaking out and surveillance.

In allusion to the practical feature of acupuncture and Tuina,by exerting the characteristic and advantages of modern teaching technique,the author carried out internet teach-ing based on Blackboard,visualized the contents of the book by effective teaching design,such as pictures,images,cartoons,voice,videos,etc.and developed video’s controlling route for teaching quality after lessons to know about students’mastery of the knowledge of cervical spondylosis and guarantee the teaching effect.

Objective:To study whether early exposure to house dust mite will aggravate airway inflammation as well as the onset of asthma and to explore the relevant mechanism.Methods:Neonate Wistar rats were injected with HDM subcutaneously instantly after birth.After the four times management,they were established as models of asthma with OVA,and bronchoalveolus was lavaged to count percentage of eosinophils.And levels of IL-4,IFN-? and serum OVA-IgE were also detected.Results:In HDM group,the percentage of EOS,level of serum OVA-IgE and level of IL-4 were (37.07?1.10) %,(0.20?0.023) and (78.96?12.18) pg/ml,respectively,the highest among the three groups,otherwise level of IFN-?,the lowest,(93.78?2.10)pg/ml.Conclusion:Early exposure to house dust mite after birth could aggravate the level of airway inflammation in later life and increase the level of IgE,which is related with T H1/T H2 imbalance.

Objective:To find the regulations of glutamine(Gln) metabolism by tumor necrosis factor-?(TNF-?).Methods:Thirty male SD rats were assigned into three groups: A(total parenteral nutrition,TPN),B(TPN+Gln),C(TPN+ TNF-?+Gln).Besides all rats were supported by isonitrogenous and isocaloric TPN solutions for 3 days,rats in group B were supplied with Gln(in the form of alanylglutamine) for 3 days at the rate of 0.3 g/(kg?d),rats in group C were infused intravenously with TNF-? at the rate of 5 ?g/(kg?h) within the last 24 hours and also supplemented with Gln for three days at the same rate as in group B.At the last 0.5 hour,all animals were mainlined with \leucine at the dosage of 1.0 mmol/kg.TNF-? levels in plasma,concentrations of Gln in plasma and skeletal muscle were measured as the experiment ended after 72 hours,and the fractional synthesis rate(FSR) were also assessed.Results:The Gln concentrations in plasma and skeletal muscle increased markedly from group A,B to C.The levels of FSR decreased remarkably along with the sequence from group B,C to A.The statistical differences were significant(P

Objective: To explore the preventive measures for early stage pulmonary complications in old patients following thoracic surgery.Methods: We conducted psychotherapy,pulmonary infection control,training in breathing and instructions in expectorating phlegm in 127 old patients(over 70 years) undergoing thoracic surgery.Results: The patients experienced difficult expectoration on the first postoperative day because of increased sputum viscoelasticity,which was gradually improved later.Analgestic and mucokinetic measures produced intended results on the first three postoperative days,with significant improvement of sputum fluidity.And the concept of fast track surgery promoted the recovery of the patients.Conclusion: The preventive measures proved essential for the prevention of early stage pulmonary complications in old patients following thoracic surgery.

OBJECTIVE To study the infection of Pseudomonas aeruginosa after brain operation and drug-sensitivity.METHODS The system of BBLCrystalTM MIND-Crystal identification and Chinese software were used to identify P.aeruginosa.Drug-sensitivity was performed by K-B methods in accordance with NCCLS.RESULTS Totally 261 specimens were isolated from 126 patients during Mar to Dec 2005.From them 99 strains of P.aeruginosa were detected from specimens of 38 patients.There were 50 strains from phlegm,20 strains from suction in trachea,6 strains from urethral catheterization,8 strains from vein catheter,and 15 strains from wound excretion.Seventy seven strains(77.7%)were isolated during the peak period of May to August.Strains that were isolated in the same period possessed uniform drug-sensitivity results,which hinted epidemic of the same clone of P.aeruginosa in the Deparment of Cerebral Surgery.Drug-sensitivity results showed P.aeruginosa had been resistant to the common antibiotics.CONCLUSIONS Infection with P.aeruginosa after brain operation and drug resistance have already been serious,so we should think seriously of it.

Objective:To construct the standard curve for real-time PCR detecting the tissue expression level of Angptl3 in SD rat.Methods:The total RNA of the SD rat's liver was the template.We got lots of the Angptl3 partial DNA sequence by RT-PCR.The products were cloned into the pUCm-T vector,Then we diluted the solution of plasmid and used real-time PCR(SYBR GreenⅠ)to construct the standard curve.Results:The correlation coefficient of the standard curve was 0.997 6.The reaction efficiency was 0.862 3.The correlation and the amplify efficiency were good.Conclusion:It is successful to construct the standard curve for real-time PCR detecting the tissue expression level of Angptl3 in SD rat.

Objective:To compare the effect of 5 methionine-related media on inhibition of gastric cancer cells' proliferation. Methods:Gastric cancer cell line SUN-16 was cultured with L-Met,Met~(-)Hcy~(-),Met~(-)Hcy~(+),D-Met,and DL-Met media respectively.The group of L-Met medium was control.A_(490) was detected by MTT in 24,48,72,96,120 h. Results:Comparing with control group,A_(490) of SUN-16 cells significantly decreased in Met~(-)Hcy~(-),Met~(-)Hcy~(+),D-Met,DL-Met media respectively((P

Death, Sudden, Cardiac ; Humans ; Reward