Objective To evaluate the role of double stapling technique in low rectal anastomosis that can save anus.Methods 114 patients with low rectal cancer from June 1994 to January 2002 were treated by low anterior resection of the rectal carcinoma in which the double stapling technique was used,were valuated and the experience was presented.Results Operations of all the 114 patients were successful. Complication included anastomotic fistula in 4 patients (3.5%)and anastomotic stenosis in 5 patients (4.4%) and they were healed by irrigating ?draining the wound and enlarging anus respectively.Conclusion The low anterior resection of the low rectal carcinoma which can save anus becomes safe and time-saving .

Objective To investigate the suppressive effect of bone marrow stromal cells modified by CTLA4Ig-gene on lymphocyte activation. Methods The expressions of CTLA4Ig-gene at transcription level and protein level were assessed by RT-PCR, immunohistochemical techniques and Dot-ELISA. The suppressive effect of CTLA4Ig on lymphocyte activation was investigated in MLR system through MTT and ELISA which quantified IL-2 level. Results The transcription of CTLA4Ig-gene could be detected by RT-PCR in the transfected group and in the passage number two. Immunohistochemistry indicated that CTLA4Ig expression was diffusely located in the cytoplasm of BMSCs. Dot-ELISA could detect the expressed CTA4Ig in the culture medium at 12h and even in culture medium of the passage number l after transfection. MLR system showed that CTLA4Ig had significant inhibition on lymphocyte proliferation and IL-2 secretion. Conclusion Ad-CTLA4Ig could transfect BMSCs efficiently. Morever, CTLA4Ig can suppress lymphocyte activation and IL-2 production.

0.05),the content ratio of glutamate to gamma-aminobutyric acid in the hippocampus of the dependent rats was lower than that in the control group(P

Traumatic rib fractures are the most common injury in thoracic trauma. Previously，the patients with traumatic rib fractures were mostly treated non-surgically，of which 50%，especially those combined with flail chest presented chronic pain or chest wall deformities and over 30% had long-term disabilities，being unable to retain a full-time job. In the past two decades，thanks to the development of internal fixation material technology，the surgical treatment of rib fractures has achieved good outcomes. However，there are still some problems in clinical treatment，including inconsistency in surgical treatment and quality control in medical services. The current consensuses on the management of regional traumatic rib fractures published at home and abroad mainly focus on the guidance of the overall treatment decisions and plans，and relevant clinical guidelines abroad lacks progress in surgical treatment of rib fractures in recent years. Therefore，the Chinese Society of Traumatology affiliated to Chinese Medical Association and Chinese College of Trauma Surgeons affiliated to Chinese Medical Doctor Association，in conjunction with national multidisciplinary experts，formulate the Chinese Consensus for Surgical Treatment of Traumatic Rib Fractures（2021）following the principle of evidence-based medicine，scientific nature and practicality. This expert consensus puts forward some clear，applicable，and graded recommendations from aspects of preoperative imaging evaluation，surgical indications，timing of surgery，surgical methods，rib fracture sites for surgical fixation，internal fixation methods and material selections，treatment of combined injuries in rib fractures，in order to provide references for surgical treatment of traumatic rib fractures.