1.The changes of force transmission to the ulnar after gradual resection of the horizontal portion of the triangular fibrocartilage complex:an experimental study
Jifeng HUANG ; Yongnian XU ; Weidong ZHAO
Chinese Journal of Orthopaedics 2001;0(08):-
0.05). Removal of two thirds or the entire horizontal portion of the TFCC caused a statistically significant difference in the percentage of force transmitted through the ulna compared with the intact force data (t=3.4,P
2.Insulin-like growth factor 1 promotes in vitro proliferation of chondrocytes in traumatic arthritis
Guang ZHAO ; Jifeng JING ; Zhiyu ZHANG ; Jiajun ZHU ; Yan CUI
Chinese Journal of Tissue Engineering Research 2014;(2):183-186
BACKGROUND:The early damaged chondrocytes are susceptible to de-differentiate and exert unstable phenotype during the in vitro culture, thus needing some growth factors.
OBJECTIVE:To observe the promotion effect of insulin-like growth factor 1 on the in vitro proliferation of chondrocytes in adult rabbits with traumatic arthritis.
METHODS:Traumatic arthritis models of adult rabbits were established by using the modified Hulth method. After the models were successful y established, the distal femur and proximal tibia were harvested under sterile conditions, the chondrocytes were cultured. The cultured cells were divided into two groups:control group was cultured with Dulbecco’s modified Eagle’s medium containing 10%fetal bovine serum, while experimental group was cultured with Dulbecco's modified Eagle’s medium containing 100μg/L insulin-like growth factor 1. The effect of insulin-like growth factor 1 on the proliferation of chondrocytes in adult rabbits with traumatic arthritis was determined through the cytomorphology, cellcounting, and cellactivity.
RESULTS AND CONCLUSION:The chondrocytes in adult rabbits with traumatic arthritis were successful y cultured, the majority of cells were mini-cells, presenting smal fusiform, round or polygonal shape. Hematoxylin-eosin staining showed that the number of cells in experimental group was higher than that in control group. MTT assay found that the absorbance of cells in experimental group was greater than that in control group (P<0.01). Our findings indicate that, insulin-like growth factor 1 can promote the in vitro proliferation of chondrocytes in adult rabbits with traumatic arthritis.
3.Application values of multislice spiral CT in children malformation of large blood vessels outside the heart
Hongwei LIANG ; Jinling ZHANG ; Deli ZHAO ; Jifeng ZHANG ; Bailu LIU
Chinese Journal of Postgraduates of Medicine 2015;38(8):554-558
Objective To analyze the application value of multislice spiral CT in diagnosis of children malformation of large blood vessels outside the heart.Methods The diagnosis results were compared between echocardiography and multislice spiral CT in 110 children with congenital heart disease,and the children had been confirmed by operation.Congenital heart disease was divided three kinds according to the region:heart,connections between heart and large blood vessels and large blood vessels outside the heart.Results One hundred and ten children with congenital heart disease were selected,and they had 366 malformations.The accuracy rates of echocardiography and multislice spiral CT were 90.7% (332/366) and 95.6% (350/366) respectively,and there was no statistical difference (x2 =3.284,P =0.070).In 366 malformations,160 malformations were in heart region.The accuracy rates of echocardiography and multislice spiral CT were 98.1% (157/160) and 97.5% (156/160) respectively,and there was no statistical difference (x2 =0.776,P =0.378).There were 55 malformations in connections between heart and large blood vessels.The accuracy rates of echocardiography and multislice spiral CT were 94.5% (52/55) and 100.0% (55/55) respectively,and there was no statistical difference (x2 =3.083,P =0.243).There were 151 malformations in large blood vessels outside the heart.The accuracy rates of echocardiography and multislice spiral CT were 81.5%(123/151) and 92.1% (139/151) respectively,and there was statistical difference (x2 =7.377,P =0.01).Conclusion Multisltce spiral CT has great application value in diagnosis of children congenital heart disease,especially in malformation of large blood vessels outside the heart.
4.The effect of atorvastatin on the expression and activity of matrix metallproteinases in rabbit abdominal arterial atherosclerosis plaque
Yulin WANG ; Xin ZHAO ; Jifeng YAN ; Xiangjun YANG ; Zhihua LIU
Chinese Journal of Geriatrics 2003;0(09):-
Objective To investigate the effect of atorvastatin on the expression and activity of matrix metalloproteinases (MMPs) in rabbit abdominal arterial atherosclerotic plaque. Methods Eighteen male New Zealand rabbits weighing 2 kg were randomized to normal control group (n=6) and hypercholesterolemia group (n=12). The latter was given hypercholesterol diet for 2 weeks, and then catheter-induced abdominal aortic wall injury was performed. Rabbits in hypercholesterolemia and aortic injury group were randomized to model group (n=6,4 weeks of hypercholesterol diet) and atorvastatin(5 mg?kg-1?d-1 for 4 weeks)group (n=6). Finally, the levels of MMP2 and MMP9 protein and mRNA in the abdominal aortic artery were measured by immunohistochemical analysis, reverse transcription-polymerase chain reaction (RT-PCR), sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) zymography. Results The intimal-medial thickness[(0. 49?0. 072) mm vs (0.66?0.079) mm, P
5.Research for IL-2R? mimic peptide on skin allograft rejection in mice
Jifeng ZHANG ; Lei ZHAO ; Bairong DU ; Xun ZHU
Chinese Journal of Immunology 1985;0(06):-
Objective:To study the effects of IL-2R? mimic peptide(CP) on skin allograft rejection in mice.Methods:Mouse skin allograft model was employed in all groups.The bioactivities were determined by lymphocyte proliferation,one-way MLR,assay of T lymphocyte subset and the level of cytokine in splenic cell culture supernatant.Results:CP inhibited lymphocyte proliferation;decreased the number of CD4+T lymphocytes in spleen and the value of CD4+/CD8+;down-regulated the level of IL-2 and IFN in splenic cell culture supernatant,as well as prolonged the mean survival times(MST) of skin allografts.The combination of CP and CsA showed cooperativities.Conclusion:CP,as the IL-2R? mimic peptide has the suppressive activities,which can efficiently inhibit skin allograft rejection in mice.
6.Research on the phytoestrogenic effect and its mechanism of isopsoralen in T47D cells
Piwen ZHAO ; Jianzhao NIU ; Jifeng WANG ; Qingxiu HAO ; Jie YU
Chinese Pharmacological Bulletin 2003;0(09):-
Aim To study the phytoestrogenic effect and its mechanism of isopsoralen in estrogen receptor (ER) positive T47D and ER negative MDA-MB231 breast cancer cell line.Methods The proliferation rate and cell cycle distribution of T47D and MDA-MB231 cells influenced by isopsoralen were analyzed by MTT and flow cytometry assay respectively.PS2 mRNA level in T47D cells was quantified by Real-time RT-PCR assay.PS2 expression was measured by flow cytometry.Estrogen receptor antagonist ICI180,782 was employed as a tool.Results The proliferation rates and indexes of T47D cells treated with 1?10-6 mol?L-1 and 1?10-7 mol?L-1 isopsoralen were increased.These effects could be blocked by ICI182,780.Meanwhile,isopsoralen didn't show stimulative effects on proliferation rate of MDA-MB231 cells.Real-time RT-PCR and flow cytometry results showed that isopsoralen at 1?10-6 mol?L-1 and 1?10-7 mol?L-1 could induce elevation of pS2 mRNA and protein expression level in T47D cells.These effects could be partly blocked by ICI182,780.Conclusion Isopsoralen has phytoestrogenic effect and its effect is attained mainly via ER pathway.
7.Gait analysis of forty-two children with spastic cerebral palsy
Jianjun LIU ; Yingyuan HU ; Jifeng ZHAO ; Xiangju GENG
Chinese Journal of Rehabilitation Theory and Practice 2001;7(4):166-167
ObjectiveTo explore the gait analysis of children with spastic cerebral palsy and its effect for the rehabilitation of cerebral palsy. Methods42 children with spastic cerebral palsy were examined with the cinematographic method and the biomechanics platform. We obtained three groups data.They are the time of gait cycle,the force in plate and the range of joints.ResultsThere was no difference in statistics within the six type cerebral palsy(P>0.05). Conclusions Children with spastic cerebral palsy are varied in the gait analysis.It is helpful to evaluate and treat cerebral palsy.
8.Manufacture of Complementary DNA Arrays on Amino-modified Slides
Bin ZHU ; Fenxiang JIN ; Jianlong ZHAO ; Yue SUN ; Jifeng CHEN ; Xintai ZHAO ; Yuansen XU
Progress in Biochemistry and Biophysics 2001;28(1):121-124
The use of rnicroarrays of oligonucleotides or cDNA is considered to be a promising approach for DNA and RNA sequence analysis, diagnostics of genetic diseases, gene polymorphism studies and analysis of gene expression. To manufacture cDNA microarrays the samples were printed onto glass microscope slides treated with poly-L-lysine, and then the slides were processed by heat and UV light treatment to attach the cDNA sequence to the glass surface. But the immobilization efficiency of cDNA on the glass surface was low. A simple procedure for manufacture cDNA microarrays on a slide treated with 3-aminopropyltrimethoxysilane is described. The efficiency for attaching cDNA to the amino-modified slides is greater than that to the slides treated with poly-L-lysine. The cDNA microarray made by the amino-modified slides is stable for use in 80℃, 75 % humidity, 3 600Lx light, exposure in air, respectively.
9.Expression of calcium handling protein in diastolic heart failure
Ming ZHONG ; Yun ZHANG ; Wei ZHANG ; Jifeng BIAN ; Zhao GENG ; Jin ZHAO
Chinese Journal of Pathophysiology 1986;0(02):-
AIM: To elucidate molecular mechanisms underlying calcium handling protein in diastolic heart failure (DHF) from mRNA level and protein expression, including calcium adenosine triphosphatase (Ca 2+ -ATPase), phospholamban, ryanodine receptor, calsequestrin and L-type calcium channel. METHODS: The mRNA of these calcium handling genes were detected by RT-PCR, and the protein levels were analyzed by Western blot analysis. RESULTS: Compared with sham-operated rabbits, the steady-state levels of mRNA encoding the SR Ca 2+ -ATPase and cardiac L-type calcium channel were decreased significantly in rabbits with DHF, and protein level of SR Ca 2+ - ATPase was greatly reduced, whereas the mRNA and protein levels of other calcium handling protein were unchanged. CONCLUSION: L-type calcium channel and the sarcoplasmic reticular Ca 2+ -ATPase were down regulated in DHF. These changes may be a contributory factor for DHF.
10.Research on the Clinical Usage of Orthosis
Yingyuan HU ; Huisan ZHAO ; Qihe CUI ; Jifeng ZHAO ; Ming YANG ; Yanchun LI ; Weihong WU ; Huabao LU
Chinese Journal of Rehabilitation Theory and Practice 1999;5(2):66-68
This research focused on the rehabilitation effect of orthosis for cerebral palsy(CP) children.46 CP children with physical dysfunction used the orthosis as an auxiliary therapy.The gait analysis,equilibrium,and comprehensive ADL measurement had been administrated pretherapy and post-therapy.The results show that 94.25% of CP children had improved their functions.The conclusions of this study indicated that it is important to use orthosis as an auxiliary therapy for CP children.Some important items of therapy had been discussed and the experiences of making plastic AFO had been introduced.