Objective To Evaluate the status of health emergency situation of disease prevention and con-trol,and to provide the basis for scientific decision -making.Methods Using the method of cross -section investiga-tion,the present situation of public health emergency in 14 public health organizations in Xi'an was investigated,the data were analyzed by Spss18.0 and Microsoft Excel 2003.Results The emergency response system was constructed basically in Xi′an.The status of health emergency control situation was not optimistic,up to standard rate of emergency equipments was low.The emergency disposal system of public health emergencies was only 14.29% in perfect.Emer-gency team received professional training accounted for 55.63%.100% of the investigation agency made the depart-ment plans according to the higher plan,but only 64.29% of the agency can do the plan for a regular exercise and maintenance.88.32% of the team members received professional training to deal with the sudden departure time from receiving an average of 0.88 hours.28.57% counties agency had no pathogenic detection equipment,14.29% county agency waswithout physical and chemical testing equipment,and 7.14% county agency had no universal equipment.The training of epidemiology,inspection and emergency management were trained on average 1.38times a year,1.2 times and 0.63 times respectively.Conclusion The emergency disposal system of the disease control institution in Xi'an has been preliminarily formed,but in the personnel configuration and other aspects exist many deficiencies.Ef-fective measures should be adopted to strengthen construction of health emergency situation.

Objective:To explore the relationship between expressions of guococorticoid receptor(GR)? and ? mRNA in peripheral blood mononuclear cell(PBMC) of patients with polymyositis/dermatomyositis(PM/DM) and the glucocorticoid(GC) efficacy.Methods:Reverse transcription-polymerase chain reaction(RT-PCR) was used to semiquantite the expressions of GR? and ? mRNA in PBMC of 18 cases of PM/DM patients sensitive to GC,10 cases of PM/DM patients resistant to GC and 20 cases of health blood donors.The levels of cortisol in sera of PM/DM patients and of health donors were detected using radioimmunoassay.Results:There was no significant difference in the levels of seral cortisol among PM/DM patients sensitive to GC, resistant to GC and the health donors.The expressions of GR? mRNA in PBMC from PM/DM patients either sensitive to GC or resistant to GC were significantly lower those that of the normal control. Moreover,the expression of GR? mRNA in PBMC of PM/DM patients resistant to GC was significantly lower than that of patients sensitive to GC. While the expression of GR? mRNA in PBMC of PM/DM patients was significantly higher than that of the normal control,there was no significant difference of the expression of GR? mRNA between PM/DM patients sensitive and resistant to GC.Conclusion:The lower expression of GR? mRNA in PM/DM patients may be related to resistance to GC therapy,whereas there is no significant role of the expression level of GR? mRNA.

BACKGROUND: Ovariectomized animals usually are employed for the study of women osteoporosis while little is known about theenergy balance of ovariectomized animals. Many previous studies showed that soy isoflavone could decrease the hyperlipemia resulted by high-fat feed, and how about the effect of soy isoflavone on the energy metabolism in ovariectomized animals?OBJECTIVE: To explore the pathologic effect of soy extract and its active components on the energy metabolism in ovariectomized rats so as to provide adequate evidences for the primary rehabilitation and prevention of type Ⅱ diabetes mellitus, disturbance of lipid metabolism as well as hypertension and coronary atherosclerotic heart disease.DESIGN: A randomized and controlled trial with the experimental animals as subjects.SETTING: Laboratory of Cell and Biochemistry in a university.MATERIALS: Ninety Wistar rats (SFP grade, license code: scxk11-00-006) were randomized into 9 groups: normal group, sham group,model group, estrogen group, high-dose soy flavone group, low-dose soy flavone group, high-dose soy extract group, low-dose soy extract group and soy polysaccharide group, with 10 rats in each group.INTERVENTION: Except the rats in normal group and sham group,the bilateral ovaries of other rats were all removed. From one week after operation, body weight and daily food intake were detected once a week.Six weeks later, the rats were killed to calculate the forage transformation efficiency, measure body length, work out the body mass index (BMI),and separate the abdominal fat and weight.MAIN OUTCOME MEASURES: ①Effect of soy extract on the abdominal fat accumulation of ovariectomized rats. ②Effect of soy extract on food intake of ovariectomized rats. ③Effect of soy extract on forage transformation efficiency of ovariectomized rats.RESULTS: After ovariectomy, the food intake, body weight and BMI all raised, and the forage transformation efficiency increased, with abdominal fat accumulation. The changes of energy metabolism induced by ovariectomy were all weakened at different degrees in estrogen group, soy extract group and soy flavone group, while no influenced was found in soy polysaccharide group.CONCLUSION: The ovariectomized rats can be used as animal model of the climacteric fat women. The soy extract, with the effective component of flavonoid, can reduce the pathologic changes such as the increase of food intake, body weight and forage transformation efficiency induced by ovariectomy.

0.05). Conclusion The g.168_185del polymorphism of the promoter in DJ-1 gene does not a significant risk factor for PD in this population.

Epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI) is one of the most important targeted drugs for lung cancer patients carrying EGFR sensitive mutations.However,almost all patients that are effective to this treatment will eventually develop secondary resistance to EGFR-TKI.The most accepted mechanisms of resistance mainly include T790M mutation,MET amplification,PIK3CA mutation,down-regulation of PTEN expression and activation of Fas-transcription factor-κB.Recent years,many new drugs are developed to overcome this resistance.Although most of drugs are in the stages of cell or animal experiment,some new drugs get positive clinical results.

EGFR-TKI plays an important role in the treatment of non-small-cell lung cancer. However, some researchers find that there are still some patients with primary or acquired resistance to EGFR-TKI. The present known mechanisms of acquired drug resistance finally lead to the re-activation EGFR downstream signa-ling pathways. Liver X receptor agonist has inhibition function to several critical steps of EGFR downstream sig-naling pathways PI3K-Akt-NF-κB,which makes it possible to overcome the drug resistance.

Objective: To observe the influence on curative effect of aescine in curing Lumbar intervertebral disc prolapse(LIDP) before and after operation. Methods: 184 cases of patients with LIDP were divided into two groups. 96 cases in curative group began to be used 10mg of aescin by adding to infusion of iv drip bid 3 days before the operation, and contie for 6-8 days it. 88 cases in control group were only operated.Results: Curative group was superior to control group in lysis of pain, postoperative La feeling and improvement of muscle strength. The excellent and better rates in two groups are 72.91%, 91.67% and 43.18%, 85.22%. The excellent rate of curative group is higher than that of control group( P 0.05). There is no serious bad reaction of drug in curative group.Conclusion: Aescin used before and after operation is good to recovery of nervous function after LIDP operation.

Objective To establish a fluorescent quantitative polymerase chain reaction method for quantifying the ICP4 gene expression of herpes simplex virus type 2(HSV2).Methods According to the HSV2 ICP4 gene sequence,we designed and synthesized PCR primer.The purified PCR product was sequenced after connecting with pMD-18 T plasmid.According to the sequence assay results,the primer and probe of fluorescent quantitative PCR was designed and synthesized.Standard recombinant plasmid extracted from the positive bacteriumclone was used as standard substance.The plasmid as standard substance was diluted for 10 times,then PCR reaction proceeded.The sensitivity and dependability of the real-time fluorescent quantitative PCR were analyzed.Results The sequence result indicated that there was non-sense mutation of A-G and T-C.And the detection sensitivity was 101 copy.The Ct value were 14.275土0.137,17.988?0.162,22.081土0.259,25.957土0.345,29.565?0.203,33.269土0.287,37.737?0.698,respectively with 107~ 101 copies/?l.The coefficient of variability were 0.965%,0.902%,1.174%,1.329%,0.686%,0.862% and1.851%,respectively.There was a good linear function in statistics between the Ct value and the concentration gradient of standard plasmid DNA specimen.The coefficient of regression was 0.998.Conclusion The method of quantification of ICP4 gene of HSV2 with real-time fluorescent quantitative PCR is successfully established,and the method has good sensitivity and dependability,which can be used to quantitative detecting HSV2 ICP4.

Objective To investigate prevalence of associated diseases in patients with gout as well as their diagnoses and treatment. Methods Two hundreds out-patients diagnosed with gout from April to October 2008 were investigated at Peking University People's Hospital, and information collected included their general characteristics, associated diseases, diagnoses and treatment, as well as blood lipid profiles, serum creatinine, uric acid, results of routine urine tests and glomerular filtration rate estimated by MDRD formula in the past three months. Results Among patients with gout, prevalence of associated hypertension, obesity, renal calculi, coronary heart disease, cerebral infarction and diabetes were 54.5% (109/200), 23.2% (42/181), 20.0% (40/200), 12.0% (24/200), 8.0% (16/200) and 7.0% (14/200), respectively, and 53.7% (101/188) of them associated with hypertriglyceridemia, 63.7% (114/179) with impaired renal function and 15.1% (27/179) with chronic kidney disease. In acute attack of gout, 124 (62.0%) of them were treated with non-steroidal anti-inflammatory drug (NASID), 72 (36.0%) with colchicines, 12 (6.0%) with corticosteroid and 30 (15.0%) with urate-lowering drugs, respectively, and during its intermittent period, 69. 8% (81/116) of them received urate-lowering therapy with indications generally accepted internationally, but serum level of uric acid could be maintained below 0.06 g/L in only 8.6% (10/116) of them. And 73.8% (48/65) of the patients with no therapy indications also were treated with urate-lowering drugs. Conclusions The most commonly associated diseases in gout patients are hypertension, hyperlipidemia and obesity, followed by renal calculi, chronic kidney disease and coronary heart disease, and so on. At present, oral NSAID is the first choice of drugs for its acute attack. Indications for urate-lowering therapy in this hospital usually are not consistent with those by generally international acceptance, with lower therapeutic effectiveness achieved.

Objective To investigate the influence and effect of treating with low dose roxithromycin on airway inflammation in asthma who have been smoking patients .Methods Forty-six patients with mild to moderate asthma who had been smoking were assigned to group A and group B randomly .The patients in group A received inhaling salmeterol/fluticasone(50/250 μg) ,one puff bid ,2 times/day .The patients in group B treated with oral roxithromycin dispersible tablet 0 .15 g/d combining with inhaling salme-terol/fluticasone(50/250 μg) ,one puff bid ,2 times/day .The patients of two groups had been treated for 4 weeks .The lung func-tion ,cells and interleukin-8(IL-8) in induced sputum of patients in the two groups were measured before and after treatment .Results There was negative correlation between the FEV 1% and the number of eosinophile granulocyte ,neutrophil and the concentration of IL-8 in induced sputum of the patients(P<0 .01) .The number of neutrophil ,eosinophile granulocyte and the level of IL-8 of the patients in group B decreased more than those in the group A after 4 weeks of treatment(P<0 .05) .Furthermore the PEF and FEV1% of the patients in group B were ameliorated significantly than those in group A (P<0 .01) .Conclusion The treatment of roxithromycin combining with salmeterol/fluticasone on patients with asthma who smoke can not only reduce the number of eosino-phile granulocyte ,neutrophil and the level of IL-8 in induced sputum significantly ,but also ameliorate the PEF and FEV1% of them significantly .