Objective To Evaluate the status of health emergency situation of disease prevention and con-trol,and to provide the basis for scientific decision -making.Methods Using the method of cross -section investiga-tion,the present situation of public health emergency in 14 public health organizations in Xi'an was investigated,the data were analyzed by Spss18.0 and Microsoft Excel 2003.Results The emergency response system was constructed basically in Xi′an.The status of health emergency control situation was not optimistic,up to standard rate of emergency equipments was low.The emergency disposal system of public health emergencies was only 14.29% in perfect.Emer-gency team received professional training accounted for 55.63%.100% of the investigation agency made the depart-ment plans according to the higher plan,but only 64.29% of the agency can do the plan for a regular exercise and maintenance.88.32% of the team members received professional training to deal with the sudden departure time from receiving an average of 0.88 hours.28.57% counties agency had no pathogenic detection equipment,14.29% county agency waswithout physical and chemical testing equipment,and 7.14% county agency had no universal equipment.The training of epidemiology,inspection and emergency management were trained on average 1.38times a year,1.2 times and 0.63 times respectively.Conclusion The emergency disposal system of the disease control institution in Xi'an has been preliminarily formed,but in the personnel configuration and other aspects exist many deficiencies.Ef-fective measures should be adopted to strengthen construction of health emergency situation.

BACKGROUND: Ovariectomized animals usually are employed for the study of women osteoporosis while little is known about theenergy balance of ovariectomized animals. Many previous studies showed that soy isoflavone could decrease the hyperlipemia resulted by high-fat feed, and how about the effect of soy isoflavone on the energy metabolism in ovariectomized animals?OBJECTIVE: To explore the pathologic effect of soy extract and its active components on the energy metabolism in ovariectomized rats so as to provide adequate evidences for the primary rehabilitation and prevention of type Ⅱ diabetes mellitus, disturbance of lipid metabolism as well as hypertension and coronary atherosclerotic heart disease.DESIGN: A randomized and controlled trial with the experimental animals as subjects.SETTING: Laboratory of Cell and Biochemistry in a university.MATERIALS: Ninety Wistar rats (SFP grade, license code: scxk11-00-006) were randomized into 9 groups: normal group, sham group,model group, estrogen group, high-dose soy flavone group, low-dose soy flavone group, high-dose soy extract group, low-dose soy extract group and soy polysaccharide group, with 10 rats in each group.INTERVENTION: Except the rats in normal group and sham group,the bilateral ovaries of other rats were all removed. From one week after operation, body weight and daily food intake were detected once a week.Six weeks later, the rats were killed to calculate the forage transformation efficiency, measure body length, work out the body mass index (BMI),and separate the abdominal fat and weight.MAIN OUTCOME MEASURES: ①Effect of soy extract on the abdominal fat accumulation of ovariectomized rats. ②Effect of soy extract on food intake of ovariectomized rats. ③Effect of soy extract on forage transformation efficiency of ovariectomized rats.RESULTS: After ovariectomy, the food intake, body weight and BMI all raised, and the forage transformation efficiency increased, with abdominal fat accumulation. The changes of energy metabolism induced by ovariectomy were all weakened at different degrees in estrogen group, soy extract group and soy flavone group, while no influenced was found in soy polysaccharide group.CONCLUSION: The ovariectomized rats can be used as animal model of the climacteric fat women. The soy extract, with the effective component of flavonoid, can reduce the pathologic changes such as the increase of food intake, body weight and forage transformation efficiency induced by ovariectomy.

EGFR-TKI plays an important role in the treatment of non-small-cell lung cancer. However, some researchers find that there are still some patients with primary or acquired resistance to EGFR-TKI. The present known mechanisms of acquired drug resistance finally lead to the re-activation EGFR downstream signa-ling pathways. Liver X receptor agonist has inhibition function to several critical steps of EGFR downstream sig-naling pathways PI3K-Akt-NF-κB,which makes it possible to overcome the drug resistance.

Epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI) is one of the most important targeted drugs for lung cancer patients carrying EGFR sensitive mutations.However,almost all patients that are effective to this treatment will eventually develop secondary resistance to EGFR-TKI.The most accepted mechanisms of resistance mainly include T790M mutation,MET amplification,PIK3CA mutation,down-regulation of PTEN expression and activation of Fas-transcription factor-κB.Recent years,many new drugs are developed to overcome this resistance.Although most of drugs are in the stages of cell or animal experiment,some new drugs get positive clinical results.

Objective:To explore the relationship between expressions of guococorticoid receptor(GR)? and ? mRNA in peripheral blood mononuclear cell(PBMC) of patients with polymyositis/dermatomyositis(PM/DM) and the glucocorticoid(GC) efficacy.Methods:Reverse transcription-polymerase chain reaction(RT-PCR) was used to semiquantite the expressions of GR? and ? mRNA in PBMC of 18 cases of PM/DM patients sensitive to GC,10 cases of PM/DM patients resistant to GC and 20 cases of health blood donors.The levels of cortisol in sera of PM/DM patients and of health donors were detected using radioimmunoassay.Results:There was no significant difference in the levels of seral cortisol among PM/DM patients sensitive to GC, resistant to GC and the health donors.The expressions of GR? mRNA in PBMC from PM/DM patients either sensitive to GC or resistant to GC were significantly lower those that of the normal control. Moreover,the expression of GR? mRNA in PBMC of PM/DM patients resistant to GC was significantly lower than that of patients sensitive to GC. While the expression of GR? mRNA in PBMC of PM/DM patients was significantly higher than that of the normal control,there was no significant difference of the expression of GR? mRNA between PM/DM patients sensitive and resistant to GC.Conclusion:The lower expression of GR? mRNA in PM/DM patients may be related to resistance to GC therapy,whereas there is no significant role of the expression level of GR? mRNA.

0.05). Conclusion The g.168_185del polymorphism of the promoter in DJ-1 gene does not a significant risk factor for PD in this population.

Objective: To observe the influence on curative effect of aescine in curing Lumbar intervertebral disc prolapse(LIDP) before and after operation. Methods: 184 cases of patients with LIDP were divided into two groups. 96 cases in curative group began to be used 10mg of aescin by adding to infusion of iv drip bid 3 days before the operation, and contie for 6-8 days it. 88 cases in control group were only operated.Results: Curative group was superior to control group in lysis of pain, postoperative La feeling and improvement of muscle strength. The excellent and better rates in two groups are 72.91%, 91.67% and 43.18%, 85.22%. The excellent rate of curative group is higher than that of control group( P 0.05). There is no serious bad reaction of drug in curative group.Conclusion: Aescin used before and after operation is good to recovery of nervous function after LIDP operation.

Objective To investigate the effect of rocuronium on spectral entropy during induction of general anesthesia in patients of Uygur nstionality. Methods Forty ASA Ⅰ or Ⅱ patients (Uygur nationality) of both sexes, aged 20-50 yr, weighing 45-70 kg, undergoing elective surgery under general anesthesia, were divided into 2 groups ( n = 20 each): normal saline (NS) group and rocuronium group (group R). Anesthesia was induced with target-controlled infusion of propofol. The initial target plasma concentration wan net at 2 μg/ml. The concentration wan then increased by 0.5 μg/ml every 4 min until response entropy (RE) was decreased to 45 and maintained for 4 min. When the plasma concentration was equal to the effect-site concentration, iv rocuronium 0.6 mg/kg was injected in group R, while group NS received the equal volume of NS instead. Fentanyl 3 μg/kg was injected intravenously at 3 min after recuronium administration. The patients were tracheal intubated and mechanically ventilated. State entropy (SE) and RE were recorded immediately before induction (baseline, To), before rocuronium administration (T1), 2 main after rocuronium administration (T2) and at 0, 1, 2 and 3 min after intubation (T3-6). The difference between RE and SE wan calculated. Results The RE value at T3 and T4 and the difference between RE and SE at T2.5 were significantly lower in group R than in group NS ( P ＜ 0.05). Conclusion Rocuronium can decrease the RE value and degree of increase in the difference between RE and SE during induction of general anesthesia in patients of Uygur nationality, which may affect the accuracy of spectral entropy in monitoring the depth of anesthesia.

Objective To detect the expressions of vascular endothelial growth factor(VEGF) and Periostin in patients with diabetic retinopathy (DR),and to explore its chnical significance.Methods A total of 52 patients with DR (DR group),36 non-DR diabetic patients (non-DR group) and 30 healthy person (normal control group) were enrolled.The 52 cases of DR patients were divided into non-proliferative DR (PDR) group (non-PDR group,24 cases) and PDR group (28 cases).The expressions of serum VEGF and Periostin were detected with enzyme-linked immunosorbent assay analysis.Results The serum expressions of VEGF and Periostin were significantly higher in non-DR group and DR group than those in normal control group [(122.63 ±28.74),(163.58 ±42.37) mg/L vs.(91.53 ± 19.58) mg/L,(110.15 ±32.62),(146.51 ± 41.74) mg/L vs.(82.26 ± 21.17) mg/L,P ＜ 0.05 or ＜ 0.01].The serum expressions of VEGF and Periostin were significantly higher in DR group than those in non-DR group (P ＜0.05).The serum expressions of VEGF and Periostin were significantly higher in PDR group than those in non-PDR group [(174.15 ±47.31) mg/L vs.(147.66 ±38.25) mag/L,(160.31 ±46.43) mg/L vs.(132.14 ±35.62)mg/L,P ＜ 0.05].The serum expression of VEGF was positively related with Periostin (r =0.415,P ＜ 0.01).Conclusion The high-expressions of VEGF and Periostin are found in DR patients,and which maybe play a key role in the early diagnosis and assessment the progression of DR patients.

Objective To investigate the influence and effect of treating with low dose roxithromycin on airway inflammation in asthma who have been smoking patients .Methods Forty-six patients with mild to moderate asthma who had been smoking were assigned to group A and group B randomly .The patients in group A received inhaling salmeterol/fluticasone(50/250 μg) ,one puff bid ,2 times/day .The patients in group B treated with oral roxithromycin dispersible tablet 0 .15 g/d combining with inhaling salme-terol/fluticasone(50/250 μg) ,one puff bid ,2 times/day .The patients of two groups had been treated for 4 weeks .The lung func-tion ,cells and interleukin-8(IL-8) in induced sputum of patients in the two groups were measured before and after treatment .Results There was negative correlation between the FEV 1% and the number of eosinophile granulocyte ,neutrophil and the concentration of IL-8 in induced sputum of the patients(P<0 .01) .The number of neutrophil ,eosinophile granulocyte and the level of IL-8 of the patients in group B decreased more than those in the group A after 4 weeks of treatment(P<0 .05) .Furthermore the PEF and FEV1% of the patients in group B were ameliorated significantly than those in group A (P<0 .01) .Conclusion The treatment of roxithromycin combining with salmeterol/fluticasone on patients with asthma who smoke can not only reduce the number of eosino-phile granulocyte ,neutrophil and the level of IL-8 in induced sputum significantly ,but also ameliorate the PEF and FEV1% of them significantly .