Objective To evaluate the medium-term follow-up outcomes of treatment of chronic scaphoid nonunion with vascularized bone graft based on 1, 2 intercompartmental supraretinacular artery.Methods From February, 1995 to November, 2006, 13 cases of chronic scaphoid nonunion were treated with vascularized bone graft based on 1, 2 intercompartmental supraretinacular artery in our hospital. Nine cases were complicated with avascular necrosis of the proximal pole. The interval between injury and treatment averaged 22. 2 months. The nonunions were stabilized by screw or external fixator. Procedures of arthrolysis were applied and early wrist mobilization was initiated under the protection of external fixator. Radiographic finding and Mayo scoring system were used to evaluate the clinical outcomes. Results The mean time of follow-up was 82.9 months. The union time for the 13 cases averaged 11.2 weeks. At the final follow-up,there were 3 excellent, 8 good and 2 fair evaluations;the range of motion of the wrist, Mayo score, grip strength, intrascaphoid angle and scapholunate angle were significantly improved. Conclusions Chronic scaphoid nonunion can be well treated with vascularized bone graft based on 1, 2 intercompartmental supraretinacular artery. A simple arthrolysis during the procedure can produce satisfactory results. External fixation plays a critical role in early mobilization and functional restoration of the wrist.

Objective To evaluate the medium and long term follow-up results of vascularized bone grafts dorsal distal radius in treatment of chronic scaphoid nonunion. Methods A retrospective study was carried out in 13 patients with chronic scaphoid nonunion treated with vascularized bone grafts dorsal distal radius in our hospital from 1995 to 2006. Nine patients were accompanied by avascular necrosis of the proximal pole. The vascular pedicle was 1,2 intercompartmental supraretinacular artery. The nonunion was stabilized by screws or external fixators. Radiographic finding and Mayo scoring system on wrist mobilization were used to evaluate the clinical outcomes. Results The interval between injury and treatment was average 22.2 months. All patients were followed up for average 76.6 months, which showed that 12 patients obtained fracture union, with average union time of 11.2 weeks. Functional evaluation results were defined as excellent in three patients, good in eight and fair in two. The last follow-up revealed significant improvement of the range of motion of the wrist, Mayo scores, grip strength, intrascaphoid angles and scapholunate angles. Conclusions Vascularized bone grafts from dorsal distal radius has relatively surgical procedure and can achieve satisfactory results in management of scaphoid nonunion. The perioperative arthrolysis and early postoperative mobilization of the wrist under exernal fixation play critical role in restoration of wrist function.

Objective To understand the status of Clonorchis sinensis infection and its risk factors in Futian District Shen?zhen City so as to provide the evidence for formulating the strategy of prevention and control. Methods Two monitoring points were randomly selected and the permanent population aged≥3 years were investigated and their stool samples were detected for the eggs of C. sinensis by using Kato?Katz technique. Meanwhile the risk factors of infection were also investigated. Results A total of 743 subjects were examined and 7 persons were infected with C. sinensis and the infection rate was 0.94%. Most of the infected cases were focused on 20?40 and 40?60 age groups. The risk factors were eating raw or semi?cooked fish and the protec?tive factor was the use of separated cutting board for cooked or uncooked food. Conclusion The infection rate of C. sinensis is low in the population of Futian District. However the health education especially for diet health education still should be strengthened.

In order to screen African swine fever virus (ASFV) diagnostic antigen with the best enzyme linked immunosorbent assay (ELISA) reactivity. By establishing the ELISA method, the diagnostic antigen of ASFV p30 protein expressed by baculovirus-insect cell expression system as reference, we explored the antigenic properties and diagnostic potential of ASFV p35 protein expressed by prokaryotic expression system as a diagnostic antigen. The results of Western blotting and immunofluorescence show that the molecular weight of the recombinant p35 protein and p30 protein obtained was 40 kDa and 30 kDa, respectively, and these two proteins had good immuno-reactivity with ASFV positive serum. Recombinant p30 and p35 proteins were used as diagnostic antigens to establish ELISA, and the sensitivity and repeatability of these methods were tested. The results show that although the detection sensitivity of the p30-ELISA established in this study was higher than that of the p35-ELISA, the sensitivity of p35-ELISA was 95.8%, and variations in intra- and inter-assay repeatability of the two methods were less than 10%. The coincidence rate between the p35-ELISA and the imported kit was 97.2%. Results show that p35-ELISA was sensitive and stable, and could detect specific antibodies against ASFV.
African Swine Fever/diagnosis* ; African Swine Fever Virus/genetics* ; Animals ; Antibodies, Viral ; Enzyme-Linked Immunosorbent Assay ; Recombinant Proteins/genetics* ; Swine