Objective To study the chemical constituents of the Dai ethno-medicine Rhinacanthus nasutus.Methods The chemical constituents had been spearated with chromatography and their structures were determined by spectral analyses.Results Nine compounds and two mixture were isolated and identified as C26—C32 alkyl mixture(Ⅰ),?-monopalmitin(Ⅱ),palmitic acid(Ⅲ),stearic acid(Ⅳ),lignoceric acid(Ⅴ),?-sitosterol(Ⅵ),lupeol(Ⅶ),p-hydroxy benzoic acid(Ⅷ),daucosterol(Ⅸ),?-ethyl-D-idosopyranoside(Ⅹ),and mixture of KNO3 and NaNO3(Ⅺ).Conclusion Compound Ⅹ is a new artificial compound during isolation and compounds Ⅰ—Ⅴ,Ⅷ—Ⅺ are isolated from R.nasutus for the first time.

Objective To observe the toxic effects of mercuric chloride (HgCl2) on the productive function of male mice with lower dosage exposune. Methods 4 week-aged male ICR mice were randomly divided into 3 exposure groups, and control group. The 3 exposure groups were treated with doses of 0.25, 0.50, 1.00 mg/kg HgCl2 by peritoneal injection respectively, one time per 3 days, 10 times in total. After exposure to HgCl2 for 50 days, the male mice were mated with female mice non-exposed to HgCl2 in a ratio 1∶2. The pregnant rate, number of pups whelped per group, body weight of offspring, testis index, sperm count, sperm motility rate, abnormal sperm rate were observed. Results The pregnant rates were 100%, 100%, 83.33% and 66.67% for control group, 0.25 mg/kg group, 0.50 mg/kg group and 1.00 mg/kg group respectively during 1-week conception, 100%, 100%, 83.33% and 75% for above corresponding groups respectively during 3-weeks conception respectively. The pregnant rate of 1.00 mg/kg group was significantly lower than that of control during 1-week conception (P

The vectors play an important role in gene therapy by transporting the therapy genes into target tumor cells. At present there are mainly two kinds of gene vectors, including biological and non-biological vectors. Biological vectors, including virus, bacteria and stem cells, have good target activity and can efficiently delivery the genes into target tumor cells, but have potential genetic hazards.However, non-biological vectors, including liposomes and nanoparticles, can be simplely prepared with high genetic safety, but they can not transport the genes efficiently. Therefore, establishing safe and effective malignant glioma gene treatment system and finding gene vectors with good target activity become the research focus.

Objective:To explore the role of MVD, bFGF and VEGF in OMFS and the relationship between them. Methods: Immunochemistry streptavidin peroxidase method was used to detect the MVD and the bFGF, VEGF expressions in 57 cases of OMFS samples. Results: MVD, bFGF and VEGF expressions were different between OMFS and benign. The close correlations were found among the expressions of VEGF, bFGF and MVD. Conclusion: The expressions of VEGF and bFGF are associated with MVD which indicated that VEGF and bFGF may play important roles in angiogenesis.

Objective To observe the effect of paraoxonase 1 (PON1) application of the new method of arylesterase activity in patients with coronary heart disease,analysis of paraoxonase 1 (PON1) of the clinical value of arylesterase activity in the new testing method.Methods From January 2014 to January 2016 in our hospital 86 patients with coronary heart disease as the research object,and then select the healthy people at the same time to the hospital physical examination of 50 as the control group to take the research object,spectrophotometric method for the determination of coronary heart disease patients and control subjects serum PON1 arylesterase activity,PON1 arylesterase activity at the same time with statistics the different degree of coronary heart disease,PON1 arylesterase activity between patients with coronary heart disease and control group comparison study and different severity of coronary heart disease patients,the patients with coronary heart disease PON1 aromatic ester enzyme activity,age,gender,BMI,TC,total cholesterol,low density lipoprotein cholesterol LDL-C and glycerin three greases TG included in the analysis of factors of coronary heart disease multiple linear regression equation,to determine the changes of patients with coronary artery disease by PON1 arylesterase activity,to provide a reference for clinical treatment.Results The activity of PON1 in patients with coronary heart disease was significantly lower than that of the control group,and the difference was statistically significant (P<0.05).Single branch lesions in patients with PON1 arylesterase activity was significantly higher than that of double vessel lesions and three lesions were double branch lesions in patients with PON1 arylesterase activity was significantly higher than that in three patients,the differences were statistically significant (P<0.05).According to the multiple linear regression analysis showed that coronary heart disease and the patient′s age,gender,BMI,TC,LDL-C,TG and PON1 arylesterase activity (P<0.05),which was related with age,gender,BMI,TC,LDL-C and TG were positively correlated,negatively correlated with PON1 arylesterase activity.Conclusion The PON1 activity of in patients with coronary heart disease is significantly decreased,and the extent of the disease is more severe,the more obvious the decline of PON1,the activity of PON1 shows a negative correlation with coronary heart disease.

0.05),that of AR 84.21% and 50.00% (P0.05). Conclusions: The pathogenesis of SS salivary glands appears to be linked to decrease or loss of topical action of androgens.Sex hormones act on epithelial cells of salivary glands through corresponding receptors.

Objective To investigate the situation of pulmonary tuberculosis among the in-school students and to provide the data for improvement of pulmonary tuberculosis control and prevention.Methods All the pulmonary tuberculosis cases of in-school student's registration cards registered during 2008 in Hefei antituberculosis station were analyzed,and the PPD and X ray were used for disease screening.Results There were 168 student patients with pulmonary tuberculosis,physical examination was the main way to find patients.The positive of mycobacterium tuberculosis examination in tuberculosis patients was highest in junior high school.The results of PPD test showed the rate of strongly positive in close contactors was 13.8%,and four new patients were diagnosed.Conclusions Study shows the pulmonary tuberculosis epidemiological and prevention condition among students sub-population are still serious,so stricter control measures should be improved in the future.

Objective To evaluate the value of multislice spiral computed tomography angiography (MSCTA) in differential diagnosis between epithelial ovarian carcinoma (EOC) and borderline epithelial ovarian tumor (BOT).Methods The MSCTA images of 39 EOC patients and 23 BOT patients confirmed by surgical pathology were reviewed retrospectively.Main characteristics of tumor vessels were analyzed: the number of feeding arteries, the existence of dilated draining veins, whether the tumor vessels were tortuous, whether the distribution of tumor vessels were disturbed, and whether there were accompanying microaneurysms or arteriovenous malformations (AVMs).Results Two or more feeding arteries of the EOCs and BOTs were 89.7% (35/39) and 8.7% (2/23), respectively.Dilated draining veins were observed in 87.2% (34/39) of the EOCs and 4.3% (1/23) of the BOTs.The tortuosity of tumor vessels was observed in 97.4% (38/39) of the EOCs and 13.0% (3/23) of the BOTs.79.5% (31/39) of the EOCs and 8.7% (2/23) of the BOTs were complicated by microaneurysms, and 74.4% (29/39) of the EOCs and 4.3% (1/23) of the BOTs were complicated by AVMs.The characteristics of tumor vessels were significantly different between the two groups (P<0.01), with relatively high sensitivity and specificity.Conclusion MSCTA can better show the distribution, number and pattern of tumor vessels and is of great value in differential diagnosis between EOC and BOT.

Objective To explore the characteristics of chromosome karyotypes in patients with chronic myeloid leukemia (CML),and to provide help to individualized treatment.Methods The date of chromosome karyotypes of 313 patients and FISH of 45 of these patients with CML excluding Ph chromosome negative (Ph-) after treatment were collected from January 2014 to June 2015.Karyotypes were detected by R-banding.Results In the 313 cases,307 cases (98.08 ％) were Ph chromosome positive (Ph+) and 6 cases (1.92 ％) were Ph-.In the Ph+ patients,288 cases (93.81％) were classical Ph+,and 19 cases (6.19 ％) were variant rearrangements.There were 48 cases (15.34 ％) with additional chromosome changes in all patients,including 41 cases (13.10 ％) with classical Ph+ and 7 cases (2.24 ％) with variant rearrangements.The most common additional chromosome changes were in the following order:+der(22) Ph (35.42 ％),+8 (33.33 ％) and +21 (12.50 ％).The most frequent pattern of combination was +der(22) combined with +8 (16.67 ％),followed by +8 combined with +21 (10.42 ％).The proportion of pure Ph+ patients in chronic phase was higher than that of advanced phase,but proportion of classical Ph+ patients with additional chromosome changes in chronic phase was lower than that in advanced phase (x2 =1 11.55,P ＜ 0.01).The proportions of chronic phase and advanced phase patients with simple variant rearrangements were not different from those with complex variant rearrangements (P =0.582).The results of FISH in 45 cases were all positive,including 5 cases with 2 GIR1Y.Conclusion Karyotype analysis can reveal the instability of genetic and the characteristics of disease progression by identifying the evolution of Ph,which provides the basis for clinical doctors to choose suitable treatment.