Objective To study the proctective effect of heme oxygeanse-1(HO-1) on cirrhotic rats after hepatic ischemia reperfusion injury.Methods All the rats were randomly divided into 5 groups as follows: normal group(N),liver cirrhosis group(LC),sham operation group(S), ischemia reperfusion group(I/R),and I/R+hemin group.Except for N group,the other groups were injected with 40% CCl4 twice a week.After 11 weeks,the model of liver cirrhosis was formed.The segmental(70%) hepatic ischemia reperfusion operation was carried out one week later.The animals were sacrificed 6h after reperfusion.Blood was collected to measure the liver function,antioxidative ability,NF-?B and caspase-3,and HO-1 expressions were studied by immunohistochemistry method. Results Administration of hemin to induce high HO-1 expression could lessen hepatic injury,increase MnSOD enzyme level,and decrease caspase-3 and NF-?B expresssion.Conclusion HO-1 plays an important role in protecting liver cirrhosis against ischemia reperfusion injury by increasing MnSOD enzyme level and decreasing expression of caspase-3 and NF-?B.

BACKGROUND:As the potent, specific immunosuppressants emerge, the survival rate after intestinal transplantation is improved to some extent. However, the adverse effects of immunosuppressants and expensive treatment costs are not tolerable for many patients. Therefore, it is clinical y meaningful to choose traditional Chinese medicine which presents immunosuppressive effects. Artesunate has immune suppression effect, reduces acute rejection fol owing smal intestine transplantation, and improves the success rate of smal intestine transplantation.
OBJECTIVE:To observe the effect and action mechanism of artesunate in acute rejection after smal intestine transplantation in rats.
METHODS:Al ogeneic smal intestine transplantation models were established in the closed group of
Sprague-Dawley rats and Wistar rats, and then were randomly divided into three groups, syngenic transplantation group (SD→SD), al ogeneic transplantation group (Wistar→SD), and artesunate treatment group (Wistar→SD+artesunate 60 mg/kg per day, intraperitoneal injection).
RESULTS AND CONCLUSION:Rats in syngenic transplantation group survived for more than 10 days and they were al kil ed on day 10. The average survival of rats in al ogeneic transplantation group and artesunate treatment group was respectively (6.73±0.58) days and (8.50±0.74) days, with significant differences between the two groups (P<0.01). Histopathological examination showed that, there was no apparent rejection in syngenic transplantation group specimens, but mild, moderate and severe rejections in al ogeneic transplantation group on days 3, 5, 7. In treatment group, some specimens had mild rejection, but appeared relatively late to a low degree. Enzyme linked immunosorbent assay results revealed that, serum interleukin-2 and interferon-gamma expression levels in al ogeneic transplantation group were significantly higher than other two groups after surgery (P<0.01), serum interleukin-2 gene expression level in treatment group was also higher than syngenic transplantation group, but there was no significant difference (P>0.05), serum interferon-gamma expression level in treatment group was higher than syngenic transplantation group (P<0.05). Artesunate can inhibit acute rejection after rat smal intestine transplantation, and its mechanism may be related to inhibition effect on the secretion and expression of interleukin-2, interferon-gamma and other cytokines.