Objective To investigate the relationship between herpes simplex virus(HSV) and spontaneous abortion. Methods 40 aborted material and 16 post-aborted curettings were tested for the presence of HSVDNA with in situ hybridization.In addition 20 normal endometria and 20 induced abortive placentas were used as controls. Results 56.3 %(9/16)post-abortion curettings were demonstrated the presence of HSVDNA.It was higher than that of the control group( P

ObjectiveTo evaluate the relationship between high sensitivity C-reactive protein(hs-CRP) level,myocardial perfusion and cardiovascular events after percutaneous coronary intervention (PCI) for ST-segment elevation acute myocardial infarction(STEMI) patients.Methods120 cases of STEMI patients received hs-CRP detection after PCI.were selected The patients were divided into the normal hs-CRP group and elevated hs-CRP group according to the hs-CRP levels.Following up for six months,the cardiovascular events of two groups were recorded.Results(1)After PCI,the percentage of TIMI myocardial perfusion grade (TMPG) 0 ～ 1 in the elevated hs-CRP group was higher than that in the normal hs-CRP group,and the percentage of TMPG 3 in the elevated hs-CRP group was lower than that in the normal hs-CRP group ( P ＜ 0.05) ; (2) Following up for six months,the incidence of cardiovascular events in the elevated hs-CRP group was significantly higher than that in the normal hs-CRP group ( P ＜0.05 ).ConclusionFor STEMI patients after PCI,the increasing of hs-CRP indicated the poorly of myocardial perfusion;the early monitoring of hs-CRP was helpful to evaluate the prognosis of acute myocardial infarction after PCI.

ObjectiveTo observe the effect of percutaneous coronary intervention(PCI) treatment on unprotected left main coronary artery(ULMCA) lesion.Methods40 patients with ULMCA lesions treated with PCI were followed up for 6 to 20 months,adverse cardiovascular events (MACE) occurrence were observed.ResultsAll patients treated by immediate PCI success rate was 100.0％.There were no serious complications.There were no serious adverse cardiovascular events during hospitalization.Patients were followed up 6 ～ 20 (7 ± 2) months,4 weeks after operation,acute myocardial infarction in 1 case,CAG confirmed circumflex artery open subacute stent thrombosis,re-PCI cure; postoperative mobilization of 28 patients took CAG in 6 ～ 12 months,including the opening circumflex artery stent restenosis in 3 cases,one distal left anterior descending branch of the trunk opening and then stenosis,2 cases of restenosis in patients with angina symptoms,3 patients re-line PCI,no need to CABG patients.No deaths during followup.The overall incidence of cardiovascular adverse events rate was 12.5％ (5/40),restenosis rate was 10.0％ (4/40).ConclusionThe choice of ULMCA lesions after PCI was safe and feasible,with good short and long term prognosis.

BACKGROUNDTo explore a rapid and accurate method for examining cancer cell from the sputum in patients with lung cancer.

METHODSOne hundred and twenty patients with lung cancer diagnosed by operation and pathologically confirmed were enrolled in this study. Sputum sediment section and sputum smear examinations were performed and compared for diagnosis of lung cancer.

RESULTS(1) The positive rate of lung cancer cell was 71.67% (86/120) by sputum sediment section examination, however, only 31.67% (38/120) by sputum smear examination ( P < 0.001). The diagnostic rate of combination of two methods for lung cancer was 90.83% (109/120), which was significantly higher than that of single sputum sediment section examination ( P < 0.001). (2) With routine HE staining, 55 patients (55/86,63.95%) could be histologically identified by sputum sediment section examination, but only 6 patients (6/38,15.79%) by sputum smear examination ( P < 0.001). (3) In 31 patients unidentified with routine HE staining, 29 were further histologically confirmed by sputum sediment section examination with immunohistochemistry.

CONCLUSIONSCompared to sputum smear examination, sputum sediment section examination can make use of more sputum materials, show a higher sensitivity for cancer cell, and accurately identify the histological classification of tumor. It is supposed to be a good examination for lung cancer and deserved to be extended in clinical application.