Objective To observe the effects of TCM preconditioning of activating blood and dissolving stasis and myocardial ischemic preconditioning on NO/serum and HSP70/myocardium of rats with ischemia reperfusion for 24 hours by ischemia reperfusion. Methods Male Wistar rats were randomly divided into 4 groups, three groups of which were fed normally, one of which was administered with suspension of Chinese medicine. 10 days later normal rats in each group were operated by coronary artery ligation to establish myocardial ischemia model. The rats of sham-operated group were performed in the same way as myocardial ischemia rats except the coronary artery was not ligated. The rats of IR group were reperfused for 24 hours after 45 min of myocardial ischemia. The rats of TCM group were treated with suspension of Chinese medicine administration of 0.32g/100g for 10 days before the same ischemia and reperfusion as IR group. And the rats of IP group were also performed the same ischemia and reperfusion as IR group after 5 min of ischemia and 5 min of reperfusion for three times. The NO/serum of rats was observed by fluorospectrophotometry and the HSP70/myocardium by immunohistochemistry. Results The results showed that the contents of NO/serum and expression of HSP70/myocardium in the IP group and TCM group of activating blood and dissolving stasis were expressed more significantly than the IP group (P<0.05) . Conclusion The group of myocardial ischemic preconditioning for ischemia reperfusion and the TCM group of activating blood and dissolving stasis have the similar effects on the contents of NO and expression of HSP70 increasing.