1.Peroxisome proliferator-activated receptor-alpha and diabetic nephropathy
Academic Journal of Second Military Medical University 1999;0(12):-
Peroxisome proliferator-activated receptor (PPAR), a member of nuclear hormone receptor superfamily, has 3 subtypes, namely, PPAR?, PPAR?/?, and PPAR?; among them PPAR? plays an important role in adipogenesis, lipid metabolism, insulin sensitivity, inflammation, atherosclerosis, and blood pressure controlling. This article reviews the relationship between PPAR? and diabetic nephropathy.
2.ADHESION OF HEAT-KILLED BIFIDOBACTERIUM ADOLESCENTIS TO HUMAN COLORECTAL CARCINOMA CELL
Yongzhi LUN ; Min HUANG ; Jieli YUAN ; Bai KANG ;
Microbiology 1992;0(04):-
The assay investigated mainly the adhesion phenomenon of heat killed Bifidoba cterium adolescentis DM8504 to human colorectal carcinoma cell line CCL 22 9 Moreover, the assay discussed the adhesion mechanism of Bifidobacterium Resu lts found heat killed bifidobacterium would be the same ability to adhere and c o lonize to human intestinal epithelial cells in vitro as live bifidobacterium, an d their adhesion had to depend on its spent culture supernatant(SCS) The adh esin of Bifidobacterium adolescentis was possibly lipoteichoic acid(LTA)w h ich existed on the cell wall of bacteria and was secreted into the SCS LTA bou nd to the heat resistant proteins of cell surface of bacteria, and extended out from the cell surface Moreover, the bifidobacterial adhesin rece ptor of intestinal epithelial cells was possibly saccharides or glycopolymers
3.Impacts on hepatitis B virus replication by gene engineering at apical loop region of capsid protein.
Jiangyan CHEN ; Rong HUANG ; Ying TAO ; Yuan HUANG ; Yingying LUO ; Ailong HUANG ; Jieli HU
Chinese Journal of Biotechnology 2013;29(11):1663-1671
Hepatitis B virus (HBV) DNA replication takes place in the viral capsid that consists of 180 or 240 copies of HBV capsid (HBc or core) protein. The monomeric core protein contains an apical loop region that forms the spikes on the surface of viral capsid upon core dimerization and capsid assembly. To investigate the impact on HBV DNA replication through gene engineering at the spike of HBV capsid. plasmids expressing engineered HBc with linker-fused enhanced green fluorescent protein (EGFP) or shortened EGFP insertion at the spike region were constructed by Restriction Digestion and Ligation-independent Cloning (RLIC). The wildtype or mutant HBc construct was cotransfected with HBV1.1c(-), a plasmid containing 1.1 unit-length HBV genome with deficiency in HBc expression, into HEK293 cells, respectively. GFP signal was observed through a fluorescence microscope and HBV DNA replicative intermediates were assayed by Southern blotting to determine the expression and functions of different recombinants. Our results demonstrated that the RLIC method was effective to generate deletion or insertion in the apical loop region of HBc. Both HBc-EGFP recombinants with different linkers produced green fluorescence but with different subcellular distribution pattern. However, HBV DNA replication was not detected with the trans-complementation of these two HBc recombinants. In addition, other recombinants including the one only with the deletion of aa79-80 failed to support HBV replication. Taken together, our results suggest that RLIC is a robust method which can be broadly applied in gene engineering; different peptide linkers may have different influences on the functions of an engineered fusion protein; and HBc aa79-80 play a critical role for HBc to support HBV DNA replication.
Capsid Proteins
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genetics
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Cloning, Molecular
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Genetic Engineering
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methods
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Green Fluorescent Proteins
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biosynthesis
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genetics
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HEK293 Cells
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Hepatitis B Core Antigens
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biosynthesis
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genetics
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Hepatitis B virus
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genetics
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physiology
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Humans
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Mutation
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Recombinant Fusion Proteins
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biosynthesis
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genetics
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Transfection
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Virus Replication
4.Comparison of osteogenic differentiation abilities of mesenchymal stem cells from different sources of hBMSCs
YUAN Lin ; QIAN Jun ; YANG Zhengyi ; WANG Han ; GUO Wucheng ; CHENG Jieli ; SONG Jingjing ; HE Enliang ; ZHANG Yi
Journal of Prevention and Treatment for Stomatological Diseases 2017;25(9):554-559
Objective:
To compare the osteogenic differentiation abilities of human bone marrow mesenchymal stem cells (hBMSCs) from different sources, and to provide basis for choosing a new source of seed cells in bone tissue engineering.
Methods:
Jaw bone-marrow-derived mesenchymal stem cells (JMMSCs) were isolated from orthognathic surgical sites and cultured by limited dilution for single cell clone. Long bone-marrow-derived mesenchymal stem cells (BMMSCs) were obtained from bone marrow of volunteers and isolated by density gradient centrifugation method. Flow cytometry was used to detect the surface markers of both cells. Osteogenic ability was assessed by PCR and Western Blot after osteogenic differentiation for the following molecules: Runx2, COL-1 and OCN. Alizarin red staining was used for determining the ability of cell mineralization after osteogenic differentiation.
Results :
The expressions of cell surface markers CD90 and CD105 were positive in both type of cells, while CD34, CD14 and CD45 were all negative. After 21 days of osteogenic induction, JMMSCs formed significantly more mineralized nodules than BMMSCs. After 7, 14, 21 days of osteogenic induction, JMMSCs expressed more osteogenic-related molecules than BMMSCs.
Conclusion
The osteogenic differentiation capacity and mineralization ability of JMMSCs are significantly higher than BMMSCs. Jaw bone might be a more suitable source of seed cells in bone tissue engineering compared with long bone.
5.K (lysine) acetyltransferase 2A affects the osteogenic differentiation of periodontal ligament stem cells through the canonical Wnt pathway.
Guo WUCHENG ; Cheng JIELI ; Yang ZHENGYI ; Zhang YI ; He ENLIANG ; Qian JUN ; Song JINGJING ; Sun JIN ; Yuan LIN
West China Journal of Stomatology 2018;36(1):39-45
OBJECTIVE:
This study aims to investigate the mechanism of K (lysine) acetyltransferase 2A (KAT2A) regulation and control on the osteogenic differentiation of periodontal ligament stem cells (PDLSCs).
METHODS:
The expression levels of KAT2A in PDLSCs were compared from each generation of the normal (H-PDLSCs) and periodontitis tissues (P-PDLSCs). The influences of KAT2A gene interference on the osteogenic differentiation of PDLSCs were also detected. In addition, the influences of the KAT2A gene interference to the canonical Wnt pathway and ligands were detected. The upstream and down-stream relationships between KAT2A and canonical Wnt pathway were also determined.
RESULTS:
The decreased expression of KAT2A in PDLSCs from the inflammatory tissue in each generation was compared with that in PDLSCs from the healthy tissue, and the difference was statistically significant (P<0.05). When the KAT2A gene was disrupted, the osteogenesis ability of PDLSC was declined, and the difference was statistically significant (P<0.05). The canonical Wnt pathway was activated, and the antagonist Dickkopf-1 (DKK-1) was reduced. After the DKK-1 addition, the osteogenic differentiation of the disturbed PDLSCs was recovered, and KAT2A was unaffected.
CONCLUSIONS
The KAT2A expression in PDLSCs was decreased because of perio-dontitis. The classical Wnt pathway was activated to inhibit the osteogenic differentiation of the cells.
Acetyltransferases
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Cell Differentiation
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Cells, Cultured
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Histone Acetyltransferases
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metabolism
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Humans
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Lysine
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Osteogenesis
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Periodontal Ligament
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metabolism
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Periodontitis
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metabolism
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Stem Cells
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Wnt Signaling Pathway