Objective To investigate the infection status and drug resistance of mycoplasmal in genitourinary tract of patients with mycoplasma infection. Methods The integration kit was adopted to perform mycoplasma culture, identification and drug sensitivity test for species from 632 patients with mycoplasma infection. Results Of all 632 patients, 285 cases were with myeoplasma infection, in which 200 cases (31.65%) were Ureaplasma urealyticum (Uu) positive, 33 cases (5.22%) were my- eoplasam hominis (Mh) positive, and 52 cases (8. 23%) were with mixed infection of Uu and Mh. The simple Uu infection had lower drug resistance against four antibiotics such as josamyein, doxycye- line, pristinamyein and elarithromycin, 2.00%, 2.50%, 4.00% and 13.00% respectively. However, it had higher drug resistance against ofloxaein and ciprofloxacin, 70. 50% and 69. 50% respectively. The resistance rate of Mh infection as well as mixed infection of Uu and Mh was much higher than that of simple Uu infection. Conclusion The detection and drug sensitivity monitoring of mycoplasma play an important role in guiding clinical treatment and controlling the production of drug resistant strains.