Objective To investigate the clinical value of HLA-B27 testing in diagnosis of ankylosing spondylitis (AS) by flow cytometry. Methods The peripheral blood HLA-B27 was detected by flow cytometry in 75 diagnosed AS patients (AS group), 95 suspected AS patients (non-AS group) and 45 health people (control group). Results The fluorescence intensity and the positive rates of HLA-B27 in AS group were significantly higher than those in non-AS group and control group: 158.0 ±24.0 vs. 94.8 ±30.5 and 89.1 ±18.9, 93.33% (70/75) vs. 5.26% (5/95) and 4.44% (2/45), and there were statistical differences (P<0.05). The sensitivity of HLA-B27 in diagnosis of AS was 93.3% (70/75), the specificity was 94.7%(90/95), while the accuracy was 94.1%(160/170). Conclusions There is a high correlation between AS and HLA-B27. The flow cytometry assay of HLA-B27 plays an important role in the early diagnosis and differential diagnosis of AS.

Objective To explored vascular epithelium growth factor (VEGF) and transforming growth factor-β1 (TGF-β1) expression profiles in rat liver regeneration after selective portal vein embolization (SPVE).Methods The SPVE model was constructed by embolization of the left portal vein and regenerative liver tissues were collected in postoperative days 1-9.Fifty of the SD rats were divided into the SPVE group (45 rats) and control group (5 rats).The expressions of VEGF and TGF-β1 in regenerative rat liver tissues were detected with immunohistochemistry and Western blot.The relationship between VEGF/TGF-β1 and time-phased rat liver regeneration was analyzed.Results In the SPVE group compared to the control group,the expression of VEGF increased from post operative day 1 to 5,peaking at day 5.The expression of TGF-β1 increased from post operative day 3 to 7,peaking at day 7.Conclusions The results suggest that after SPVE,VEGF fluctuation may correlate with the start of hepatic regeneration,and TGF-β1 fluctuation may correlate with the termination of hepatic regeneration.

Objective To study the therapeutic effects of omeprazoie in high-dose given by continuous intravenous infusion in the treatment of stress-related mucosal injury of G-I tract in intensive care patients.Method Totally 98 intensive care patients with stress-related mucosal injury(SRMI)were enrolled from August 2006 to October 2008 Department in Intensive Care Unit(ICU)of the Provincial Hospital Affiliated to Shandong University.All the patients were randomly divided into high-dose omeprazoie group(group A)and control group(group B).In group A,omeprazoie was administrated in loading dose of 80 mg Ⅰ.Ⅴ.in 5 minutes followed by maintenance dose of 8.0 mg/h intravenous infusion for 72 hours,while in group B,omeprazoie was given in dose of 40mg/8h intravenous infusion for 72 hours.The pH value of gastric juice was determined by German Roche pH test paper every 2 to 8 hours in the patients of both groups.The coffee like or red juice in the gastrointestine decompressor was observed.At the same time,hemoglobin(HB)was detected by Automatic blood cell analyzer Sysmex XE-2100,blood urea nitrogen(BUN)was determined by Automatic Analyzer Au5400,and buffer excess(BE)was checked by GEM Premie arterial blood gas analyzer in all patients.Data were expressed as mean ± standard deviation(x-± s)and the analysis of variance was done with SPSS 12.0 software.Comparison of mean value between two groups was conducted with t-test and the ratio was calculated by using chi-square test(X2 test).The change was considered as statistically significant if P value was less than 0.05.Results Four,eight,and 24 hours after treatment with omeprazole,the pH values in patients of group A were higher than those in patients of group B(four hous:6.63 ±0.62 vs.3.14 ±0.26,P＜0.01;eight hours and 24 hours:P＜0.05 or P＜0.01).At 8 hours and 24 hours after treatment,the HB was higher,BUN and BE were lower in group A than those in group B(P＜0.03 or P＜0.01).The total rate of hemostasis of upper G-I tract bleeding in group A was higher than that in group B(95.35%vs.78.19%,P＜0.05).Conclusions For treating the intensive care patients with SRMI,the continues intravenous infusion of omeprazole inhigh dose is superior to conventional dosage.

Objective:To investigate the clinical characteristics and risk factors of acute ischemic stroke (AIS) complicated with cerebral cardiac syndrome (CCS).Methods:The clinical data of AIS patients admitted to the ICU of our hospital from January 2019 to May 2021 were collected and retrospectively analyzed. According to whether CCS occured after stroke, the patients were divided into the CCS group and control group. Binary logistic regression analysis was used to analyze the clinical characteristics of AIS combined with CCS and the risk factors for the occurrence of CCS.Results:A total of 196 patients with AIS were included, and 92 patients had CCS, of which 74 occurred within 72 h after AIS. Binary logistic regression analysis showed that old age ( OR=1.15, 95% CI: 1.01-1.23), hypertension ( OR=1.41, 95% CI: 1.26-1.58), diabetes ( OR=2.05, 95% CI: 1.86-2.24), chronic kidney disease ( OR=1.36, 95% CI: 1.22-1.48), chronic obstructive pulmonary disease ( OR=1.46, 95% CI:1.22-2.48), smoking ( OR=1.52, 95% CI:1.23-1.78), higher NISSH score ( OR=1.36, 95% CI: 1.29-1.48), brainstem infarction ( OR=2.33, 95% CI: 2.21-2.50), thalamic infarction ( OR=1.21, 95% CI: 1.17-1.28), and hyperlipidemia ( OR=1.86, 95% CI: 1.61-1.96) were associated with the occurrence of CCS after stroke. Conclusions:CCS is a common complication in patients with AIS, and mostly occurs within 72 hours after stroke. The risk of CCS is not only related to the history of hypertension, diabetes, chronic kidney disease, smoking history and hyperlipidemia, but also related to the location of infarction and the degree of nerve function injury. The changes of cardiac function in patients with AIS should be closely monitored to prevent cardiac events.

Objective To investigate correlation between genetic polymorphisms of CDHR3 (rs6967330),GSDMB (rs2305480),IL-33 (rs928413),RAD50 (rs6871536) and IL1RL1 (rs1558641) and occurrence and severity of allergic asthma in Han population.Methods Genotype and allele frequencies were compared between 516 patients and 552 controls by Chi-square test.Correlation between genotype and FEV1,total IgE was analyzed.Results Compared with the controls,the allergic patients had significantly higher allergic G of rs928413 and allergic C of rs6871536 (P ＜0.001).Besides,allergic patients were found to have significantly lower frequency of allergic A of rs1558641 (P =0.007).Compared with other genotypes,patients with rs928413 genotype GG and rs1558641 genotype GG were significantly correlated with low FEV1％ and high level of serum total IgE.Conclusion Gene IL-33,IL1R1,and RAD50 are correlated with the risk of asthma in Han population.

Objective To investigate correlation between genetic polymorphisms of CDHR3 (rs6967330),GSDMB (rs2305480),IL-33 (rs928413),RAD50 (rs6871536) and IL1RL1 (rs1558641) and occurrence and severity of allergic asthma in Han population.Methods Genotype and allele frequencies were compared between 516 patients and 552 controls by Chi-square test.Correlation between genotype and FEV1,total IgE was analyzed.Results Compared with the controls,the allergic patients had significantly higher allergic G of rs928413 and allergic C of rs6871536 (P ＜0.001).Besides,allergic patients were found to have significantly lower frequency of allergic A of rs1558641 (P =0.007).Compared with other genotypes,patients with rs928413 genotype GG and rs1558641 genotype GG were significantly correlated with low FEV1％ and high level of serum total IgE.Conclusion Gene IL-33,IL1R1,and RAD50 are correlated with the risk of asthma in Han population.

Purpose: Microtubule-associated protein 1 light chain 3B (LC3B) serves as a key component of autophagy,which is associated with the progression of carcinoma. Yet, it is still unclear whetherLC3B is also an independent risk factor for intrahepatic cholangiocarcinoma (ICC). We aimto explore the predictive value of LC3B on prognosis of ICC, and to establish a novel andavailable nomogram to predict relapse-free survival (RFS) and overall survival (OS) for thesepatients after curative-intent hepatectomy. Materials and Methods: From August 2004 to March 2017, 105 ICC patients were eligibly enrolled in the ThirdAffiliated Hospital of Sun Yat-sen University. Preoperative clinical information of enrolledpatients was collected. Expression LC3B in the ICC specimen was detected by immunohistochemistry. Results: The 5-year RFS and OS in this cohort were 15.7% and 29.6%, respectively. On multivariateCox regression analysis, independent risk factors for 5-year OS were cancer antigen 125,microvascular invasion, LC3B expression and lymph node metastasis. Except for the above4 factors, neutrophil/lymphocyte ratio and tumor differentiation were independent factorsfor 5-year RFS. The area under the curve of nomograms for OS and RFS were 0.820 and0.747, respectively. Conclusion The nomograms based on LC3B can be considered as effective models to predict postoperativesurvival for ICC patients.

Objective:To investigate the effect of acute hypervolemic hemodilution (AHH) with 6% hydroxyethyl starch 130/0.4 on pharmacodynamics of propofol during successful laryngeal mask airway (LMA) implantation.Methods:American Society of Anesthesiology physical status Ⅰ or Ⅱ patients, aged 30-60 yr, with body mass index of 18.5-25.0 kg/m 2, undergoing elective extensive total hysterectomy under general anesthesia, were divided into 2 groups: AHH group (group A) and control group (group C). In group A, 6% hydroxyethyl starch 130/0.4 was infused at a rate of 20 ml/min for AHH, and the target hematocrit was 30%.In group C, lactated Ringer′s solution was infused according to the " 4-2-1" rule to supplement physiological requirements, and anesthesia induction was performed after 10 min of stabilization.Sufentanil was administered by target-controlled infusion using Bovil pharmacokinetic model with effect-site concentration (Ce) of 0.25 ng/ml, 3 min later propofol was given by target-controlled infusion using Schnider model.The Ce of propofol in the first patient was set at 5.0 μg/ml.Each time the concentration of propofol was increased/decreased by 0.5 μg/ml according to the sequential method.LMA was inserted following 1 min equilibration between plasma concentration and Ce of propofol.The trial was terminated when 8 consecutive inflection points of failed/successful LMA insertion occurred.The EC 5, EC 50, EC 95 and 95% confidence interval (95% CI) of propofol were calculated by probit regression analysis. Results:In group A, the EC 5 (95% CI), EC 50 (95% CI) and EC 95 (95% CI) of propofol when LMA was successfully placed were 4.237 (3.090-4.514) μg/ml, 4.802 (4.500-5.078) μg/ml and 5.443 (5.125-7.304) μg/ml, respectively.In group C, the EC 5 (95% CI), EC 50 (95% CI) and EC 95 (95% CI) of propofol when LMA was successfully placed were 2.408 (1.190-2.756) μg/ml, 3.120 (2.690-3.472) μg/ml and 4.042 (3.582-7.431) μg/ml, respectively.There was significant difference in EC 5, EC 50 and EC 95 between the two groups ( P<0.01). Conclusion:AHH with 6% hydroxyethyl starch 130/0.4 can decrease the efficacy of propofol when LMA is successfully implanted.

Objective:To evaluate the efficacy and safety of less invasive surfactant administration (LISA) combined with nasal intermittent positive pressure ventilation (NIPPV) in the treatment of infants with respiratory distress syndrome (RDS).Methods:A prospective study was conducted on preterm infants of gestational age ≤34 weeks with RDS who were admitted to the Neonatal Intensive Care Unit of Xuzhou Central Hospital from October 2019 to November 2021. The infants were randomly assigned into the LISA+NIPPV group and the intubation-surfactant-extubation (INSURE) +nasal continuous positive airway pressure (NCPAP) group. In the LISA+NIPPV group, with the support of NIPPV, a Lisa tube was inserted through the vocal cords under direct vision with direct laryngoscope, and then pulmonary surfactant (PS) was infused into the lung. In the INSURE+NCPAP group, the patients were endotracheally intubated and infused with PS into the lung through endotracheal tube, then extubated and continued to receive NCPAP therapy (INSURE). The blood gas analysis at 1 h and 6 h after PS infusion, the adverse reactions during injection, clinical efficacy, bronchopulmonary dysplasia (BPD) and other related complications were compared between the two groups.Results:A total of 112 preterm infants with RDS were enrolled, including 58 in the LISA+NIPPV group and 54 in the INSURE+NCPAP group. The blood oxygen partial pressure (PaO 2) and PaO 2/FiO 2 (P/F) in the LISA+NIPPV group were significantly higher than those in the INSURE+NCPAP group at 1 h and 6 h after PS infusion, while carbon dioxide partial pressure (PaCO 2) were significantly lower than that in the INSURE+NCPAP group, and the differences were statistically significant (all P<0.05). The rate of tracheal intubation within 72 h (15.5% vs. 33.3%), the duration of non-invasive ventilation [ (7.5 ± 4.3) d vs.(9.9 ± 5.5) d ], total oxygen inhaling [ (10.5 ± 3.5) d vs.(13.3 ± 4.1) d ], failure rate of machine withdrawal (8.6% vs. 31.0% ), the times of apnea [7.0 (3.0-21.0) times vs. 15.0 (4.0-28.0) times ] and re-administration of PS (17.2% vs. 33.3%) in the LISA+NIPPV group were significantly lower than those in the INSURE+NCPAP group, and the differences were statistically significant ( P<0.05). The incidence of regurgitation in the LISA+NIPPV group was lower than that in the INSURE+NCPAP group (13.8% vs. 35.2%), and the difference was statistically significant ( P<0.05). There was no significant difference in the time needed for intubation between the two groups ( P>0.05). The occurrence of BPD in the LISA+NIPPV group was significantly lower than that in the INSURE+NCPAP group (10.3% vs. 25.9%), and there was no significant difference in other related complication between the two groups (all P>0.05). Conclusions:LISA combined with NIPPV in the treatment of preterm infants with RDS can effectively improve oxygenation, reduce carbon dioxide retention, reduce the mechanical ventilation rate, shorten the duration of noninvasive mechanical ventilation, and reduce the incidence of BPD.