Objective:To introduce an economical and pragmatic method of establishing oxygen-glucose deprivation model of hippocampal slice in neonatal rats in vitro,Methods:Hippocampal slices(400?m)from 8~10-day-old rats are transferred into an incubator with insert of Millicell membrance and incubated for 7 d,14 d,20 d and 30 d,respectively.The growing state of the hippocampal slices were observed under the convened microscope,and its ability to absorb propidiumiodide (PI) was detected under fluorescent microscope after the hippocampal slice was stained by PI,and the state of the hippocampal slices at different time after aerating Nitrogen gas was observed. Results: ① No PI labelled cells were found after 7 d and 14 d. However,15% PI positive cells were found after 30 d. ②The longer in Nitrogen gas,the more apoptosis cells. ③Intensive fluorescence was observed in the CA1,CA3 and DG areas of the hippocampal slice after aerating Nitrogen gas for 1.5h. Conclusion:The hippocampal slice can be alive for about 30 days.Stable oxygen-glucose deprivation model of the hippocampl slice in vitro was successfully established after aerating Nitrogen gas for 1.5 h.

Purpose: Repopulation is among those which causes treatment failure in cancer radiotherapy. The study focuses on a better understanding of radiation-induced repopulation for cancer cells.Materials and Methods: It was measured by BrdUrd/DNA flow cytometric analysis that the kinetic changes after various radiation dose-time effects on human nasophayngeal carcinoma cells. The surviving fraction of the clonogenic cells was also measured by limiting dilution method.Results: (1) the shortening of Tpot of overall cell population after 0～8 Gy of irradiation was closely related to the decrease of the clonogenic cells;(2) an increased DNA synthesis was suggested as a result of the increase of radiation dose and (3) in vitro ,the proliferative status of the clonogenic cells was as same as that of overall cell population 48 hours after irradiation.Conclusion: (1) the accelerated repopulation of CNE cells after irradiation is related to their self-regulation;and (2) Tpot may be used as a predictive value in clinical radiotherapy.

Objective:To introduce an economical and pragmatic method of establishing oxygen-glucose deprivation model for hippocampal slice of neonatal rats in vitro. Methods: Hippocampal slices(400?m) from 8~10-day-old rats were transferred into an incubator with insert of Millicell membrance and incubated for 7d,14d,20d and 30d respectively.Then they were observed under convened microscope to evaluate their growing state,and detected their ability to absorb propidiumiodide(PI) under fluorescent microscope after they had been stained by PI , The state of the hippocampal slices was observed at different time after aerating Nitrogen gas. Results:(1)No PI labelled cells were found after 7d and 14d.However,15% PI positive cells were found after 30d.(2)The longer in Nitrogen gas ,the more apoptosis cells.(3)Intensive fluorescence was observed in the CA1, CA3 and DG areas of the hippocampal slice after aerating Nitrogen gas for 1.5h.Conclusion:The hippocampal slice can be alive for about 30 days.Stable oxygen-glucose deprivation model of the hippocampal slice in vitro was successfully established after aerating Nitrogen gas for 1.5h.

Carcinoma ; classification ; pathology ; Consensus ; Humans ; Thymoma ; classification ; pathology ; Thymus Neoplasms ; classification ; pathology ; World Health Organization

Objective To compare the efficacy and incidence of complications of stenting alone and intratumoral injection of recombinant human adenovirus p53 combined with stent implantation in the treatment of advanced esophageal cancer patients.Methods 2 4 cases with advanced esophageal cancer,who could only eat semi liquid,liquid food or could not eat,were randomly divided into control group(A group) and treatment group (B group).12 cases in A group were treated with esophageal stent implantation,12 cases in B group were given endoscopic intratumor injection of recombinant human adenovirus p53 combined with food tract stent implantation.Results The rate of improvement of dysphasia had no significant difference in survival period:the treatment group survival time of 3.2-38 months,mean (27.0 ± 2.3) months,while the control group survival time of 2.8-27months,an average of (14.7 ± 8.0) months,the difference between the two groups was significant (t =3.521,P ＜ 0.05).The incidence rate of restenosis in treatrnent group was 8.3％,that in control group was 41.7％.There was significant difference (x2 =7.532,P ＜0.05).Conclusion Endoscopic intratumor injection of recombinant human adenovirus p53 combined with esophageal stent implantation in the treatment of advanced esophageal carcinoma can prolong the survival time of patients with esophageal stent implantation,and to reduce the incidence of restenosis.

Objective To investigate the effects of homemade tirofiban on activated-platelet and prethrombotic state in rabbit model of iliac artery injury by balloon angioplasty.Methods Forty New Zealand white rabbits,lavaged with aspirin(12 mg/kg)and clopidogrel(16mg/kg)8～12hs before the experiment,followed by abrosia,were divided into 3 groups.The iliac artery injury models were set up successfully only in 30 out of the forty rabbits.Rabbits in the pre-treatment group(n=11)received homemade tirofiban right after being lavaged and the drug was given to the treatment group(n=13)later after the iliac artery was injured by PTCA balloon.Placebo was given to the control(n=6)after the injury.Blood samples were drawn before and after the procedure for platelet aggregation,sP-selectin and 6-Keto-PGF1? analysis.Changes in iliac systolic blood pressure(SBP)and diastolic blood pressure(DBP)were monitored throughout the operation.Iliac arteriography and pathological study were carried out in some animals for analysis of the composition of thrombus.Results(1)The sP-selectin levels were different between the pre-treatment group and the treatment group(58.1?26.2 ?g/L vs 24.8?14.3 ?g/L pre-operation;53.2?40.2 ?g/L vs 53.5?27.7 ?g/L post-operation,respectively,P

Objective To study the role of Schwann cells apoptosis in the pathogenesis of experimental autoimmune neuritis(EAN) in TNF receptor Ⅰ knock out(TNFR Ⅰ-/-)mice.Methods For induction of EAN,TNFRⅠ-/- and wild type C57BL/6 mice were immunized by subcutaneous injection into the back with the peripheral nerve P0 protein peptide 180-199;clinical scores of EAN were assessed and scored immediately before immunization(day 0) and thereafter every other day until day 46 post immunization(p.i.).On day 22 p.i.,Schwann cells were collected from the two groups and cultivated in vitro.The expressions of Fas and Annexin-Ⅴ(Annexin-Ⅴ+/PI-)on Schwann cells from TNFRⅠ-/-and wild type EAN were detected by flow cytometry.Results More light clinical signs of EAN were observed in the TNFRⅠ-/-mice(1.50?0.19) than in wild type mice(1.90?0.16);the percentage of Fas expression on Schwann cells was significantly decreased in TNFRⅠ-/-mice(88.03%?1.40%)as compared with wild type mice(94.70%?1.53%)(P

OBJECTIVE:To establish the evaluation index system that is suitable for biological pharmaceutical industry cluster competitiveness,and provide reference for the evaluation of biological pharmaceutical industry cluster competitiveness in Jiangsu province. METHODS:Biological pharmaceutical industry cluster enterprises in Jiangsu province were selected as research samples. Through the questionnaires,the grey multi-index evaluation method was used to determine weight set,establish evaluation sample matrix,and evaluate and analyze 3 first-level indexes(8 second-level indexes,and 23 tertiary-level indexes),such as the innova-tion knowledge spillover,innovation network and social capital,etc. RESULTS & CONCLUSIONS:The financial indicators such as investment intensity of biological pharmaceutical industry cluster in Jiangsu province were low,and there was a shortage of funds problem;new drug patents of independent intellectual property rights were insufficient,and lacked of protection of intellectu-al property rights,there was an independent innovation motivation problem;profitability indexes such as the rate of product sales were low,there was a problem of low industrialization of scientific research;social culture score in social capital level was not high,there was a problem of strengthening soft environment such as the binding biological innovation public service platform. It is suggested to strengthen the cooperation among cluster enterprises and scientific research institutions,and improve enterprise innova-tion power;improve the mechanism of biological innovation and the level of industrialization of scientific research achievements;and actively guide the various types of biological innovation public service platform to participate in the construction of the soft en-vironment of the cluster.

Objective To evaluate the clinical effectiveness and pseudoaccommodation in the early stage after AcrySof ReSTOR multifocal intraocular lens (IOL) and monofocal IOL implantation.Methods A total of 138 patients (158 eyes) with age-related cataract underwent phacoemulsification and IOL implantation were chosen,73eyes of 60 patients with a multifocal IOL implantation as observation group and 85 eyes of 78 patients with monofocal IOL implantation as control group.The uncorrected visual acuity,best corrected visual acuity was evaluated at 1 week,1 month,and 3 months after operation,pseudoaccommodation and quality of life was evaluated at 3 months after operation.Results The patients of both observation group and control group were able to obtain a satisfactory distance vision at 1 week,1 month,and 3 months.The uncorrected intermediate visual acuity of observation group were 0.49 ± 0.19,0.72 ± 0.21,0.77 ±0.23,which of control group were 0.24 ± 0.21,0.27 ± 0.22,0.28 ± 0.24,there were statistical differences between two groups (all P ＜ 0.05).The uncorrected near visual acuity of observation group were 0.47 ± 0.20,0.70 ± 0.22,0.80 ± 0.24,which of control group were 0.21 ±0.23,0.23 ±0.19,0.26 ±0.18,there were statistical differences between two groups (all P ＜ 0.05).There was no significant difference in the uncorrected near visual acuity,best corrected distance,intermediate and near visual acuity between observation group and control group (all P ＞ 0.05).At 3 months,the distance and near pseudoaccommodation of observation group were (2.56 ± 0.82) D,(3.19 ±1.13)D,which of control group were (0.87 ±0.57)D,(1.03 ±0.59) D,there were statistical differences between two groups (all P ＜ 0.05).At 3 months,VF-14 scale score and wearing glasses as near score of observation group were 92.21 ±4.22,4.23 ±0.85,which of control group were 71.23 ± 3.96,2.01 ± 0.71,there were statistical differences between two groups (all P ＜ 0.05).Conclusion AcrySof ReSTOR multifocal intraocular lens implantation can provide a higher level of visual acuity,pseudoaccommodation and satisfaction compared with monofocal intraocular lens.

Lysyal oxidase (LOX),a cooper dependent monoamine oxidase,can stabilize the extracellular matrix and is closely related to the tumor cell differentiation,vicious transformation and invasiveness.LOX is secreted into extracellular,and it is resolved into a small fragment by sol protease,which is lysyal oxidase propeptide (LOX-PP).According to research,LOX-PP has its own structure and physiological functions,which can inhibit the growth of tumor cells.LOX-PP also plays a significant role in the generation and progression of tumor,which is likely to be a new therapeutic target.