Objective:To introduce an economical and pragmatic method of establishing oxygen-glucose deprivation model for hippocampal slice of neonatal rats in vitro. Methods: Hippocampal slices(400?m) from 8~10-day-old rats were transferred into an incubator with insert of Millicell membrance and incubated for 7d,14d,20d and 30d respectively.Then they were observed under convened microscope to evaluate their growing state,and detected their ability to absorb propidiumiodide(PI) under fluorescent microscope after they had been stained by PI , The state of the hippocampal slices was observed at different time after aerating Nitrogen gas. Results:(1)No PI labelled cells were found after 7d and 14d.However,15% PI positive cells were found after 30d.(2)The longer in Nitrogen gas ,the more apoptosis cells.(3)Intensive fluorescence was observed in the CA1, CA3 and DG areas of the hippocampal slice after aerating Nitrogen gas for 1.5h.Conclusion:The hippocampal slice can be alive for about 30 days.Stable oxygen-glucose deprivation model of the hippocampal slice in vitro was successfully established after aerating Nitrogen gas for 1.5h.

Objective:To introduce an economical and pragmatic method of establishing oxygen-glucose deprivation model of hippocampal slice in neonatal rats in vitro,Methods:Hippocampal slices(400?m)from 8~10-day-old rats are transferred into an incubator with insert of Millicell membrance and incubated for 7 d,14 d,20 d and 30 d,respectively.The growing state of the hippocampal slices were observed under the convened microscope,and its ability to absorb propidiumiodide (PI) was detected under fluorescent microscope after the hippocampal slice was stained by PI,and the state of the hippocampal slices at different time after aerating Nitrogen gas was observed. Results: ① No PI labelled cells were found after 7 d and 14 d. However,15% PI positive cells were found after 30 d. ②The longer in Nitrogen gas,the more apoptosis cells. ③Intensive fluorescence was observed in the CA1,CA3 and DG areas of the hippocampal slice after aerating Nitrogen gas for 1.5h. Conclusion:The hippocampal slice can be alive for about 30 days.Stable oxygen-glucose deprivation model of the hippocampl slice in vitro was successfully established after aerating Nitrogen gas for 1.5 h.

Purpose: Repopulation is among those which causes treatment failure in cancer radiotherapy. The study focuses on a better understanding of radiation-induced repopulation for cancer cells.Materials and Methods: It was measured by BrdUrd/DNA flow cytometric analysis that the kinetic changes after various radiation dose-time effects on human nasophayngeal carcinoma cells. The surviving fraction of the clonogenic cells was also measured by limiting dilution method.Results: (1) the shortening of Tpot of overall cell population after 0～8 Gy of irradiation was closely related to the decrease of the clonogenic cells;(2) an increased DNA synthesis was suggested as a result of the increase of radiation dose and (3) in vitro ,the proliferative status of the clonogenic cells was as same as that of overall cell population 48 hours after irradiation.Conclusion: (1) the accelerated repopulation of CNE cells after irradiation is related to their self-regulation;and (2) Tpot may be used as a predictive value in clinical radiotherapy.

Carcinoma ; classification ; pathology ; Consensus ; Humans ; Thymoma ; classification ; pathology ; Thymus Neoplasms ; classification ; pathology ; World Health Organization

Lysyal oxidase (LOX),a cooper dependent monoamine oxidase,can stabilize the extracellular matrix and is closely related to the tumor cell differentiation,vicious transformation and invasiveness.LOX is secreted into extracellular,and it is resolved into a small fragment by sol protease,which is lysyal oxidase propeptide (LOX-PP).According to research,LOX-PP has its own structure and physiological functions,which can inhibit the growth of tumor cells.LOX-PP also plays a significant role in the generation and progression of tumor,which is likely to be a new therapeutic target.

Objective To investigate the effects of homemade tirofiban on activated-platelet and prethrombotic state in rabbit model of iliac artery injury by balloon angioplasty.Methods Forty New Zealand white rabbits,lavaged with aspirin(12 mg/kg)and clopidogrel(16mg/kg)8～12hs before the experiment,followed by abrosia,were divided into 3 groups.The iliac artery injury models were set up successfully only in 30 out of the forty rabbits.Rabbits in the pre-treatment group(n=11)received homemade tirofiban right after being lavaged and the drug was given to the treatment group(n=13)later after the iliac artery was injured by PTCA balloon.Placebo was given to the control(n=6)after the injury.Blood samples were drawn before and after the procedure for platelet aggregation,sP-selectin and 6-Keto-PGF1? analysis.Changes in iliac systolic blood pressure(SBP)and diastolic blood pressure(DBP)were monitored throughout the operation.Iliac arteriography and pathological study were carried out in some animals for analysis of the composition of thrombus.Results(1)The sP-selectin levels were different between the pre-treatment group and the treatment group(58.1?26.2 ?g/L vs 24.8?14.3 ?g/L pre-operation;53.2?40.2 ?g/L vs 53.5?27.7 ?g/L post-operation,respectively,P

Objective To study the role of Schwann cells apoptosis in the pathogenesis of experimental autoimmune neuritis(EAN) in TNF receptor Ⅰ knock out(TNFR Ⅰ-/-)mice.Methods For induction of EAN,TNFRⅠ-/- and wild type C57BL/6 mice were immunized by subcutaneous injection into the back with the peripheral nerve P0 protein peptide 180-199;clinical scores of EAN were assessed and scored immediately before immunization(day 0) and thereafter every other day until day 46 post immunization(p.i.).On day 22 p.i.,Schwann cells were collected from the two groups and cultivated in vitro.The expressions of Fas and Annexin-Ⅴ(Annexin-Ⅴ+/PI-)on Schwann cells from TNFRⅠ-/-and wild type EAN were detected by flow cytometry.Results More light clinical signs of EAN were observed in the TNFRⅠ-/-mice(1.50?0.19) than in wild type mice(1.90?0.16);the percentage of Fas expression on Schwann cells was significantly decreased in TNFRⅠ-/-mice(88.03%?1.40%)as compared with wild type mice(94.70%?1.53%)(P

Aiming at the technical characteristics and transforming difficulties for patents in medical health industry,this paper makes proposition on establishing a service system for patent transformation in healthcare system and accelerating the transformation and clinical application of medical technologies through the enhancement of technical service platform construction,strengthening of professional staff capabilities,and the improvement of patent values assessment.

This study aimed to explore clinical features of multiple myeloma in patients over 80 years old. Methods: We retrospectively analyzed 11 cases of multiple myeloma over 80 years old, which were diagnosed from 2000 to 2013 in our hospital. Results:The mean age was 83.5 years. All patients had more than two complicated diseases, and the performance status was poor in the majority of the patients. Ten cases received individualized treatments. The results showed that three patients had progressed, one patient achieved complete remission, three patients achieved partial remission, and four patients achieved minor remission. Median survival time was 28 months. The causes of death included six progressed myelomas, three pneumonias, and two acute myocardial infarctions. Conclusion: Patients aged 80 years old with myeloma are often at a late stage. Treatment should be individualized based on patient status. Survival time was evidently prolonged in very elderly patients with MM after individualized treatments, particularly in patients in stagesⅠandⅡ.

AIM:To observe the effects of edaravone on high glucose-induced apoptosis of SH-SY5Y cells and its potential mechanism .METHODS:The SH-SY5Y cells were cultured in the DMEM medium with 100 mmol/L glucose and 100μmol/L edaravone for 24 h.The viability of the SH-SY5Y cells was detected by MTT assay .The levels of ROS in the cells were determined by DCFH-DA fluorescent probing .The apoptotic rates of the cells were analyzed by flow cytome-try.The protein expression of Bax and Bcl-2 in the cells were detected by Western blot .The expression levels of micro-RNA-25 (miR-25) were determined by real-time PCR.To further clarify the target sites of edaravone on inhibiting apopto-sis induced by high glucose , miR-25 inhibitor was applied to the SH-SY5Y cells and the activity of caspase-3 was meas-ured.RESULTS:Compared with control group , the cell viability was decreased significantly in model group , and the ROS level was increased significantly .The protein expression of Bax was up-regulated significantly , while the expression levels of Bcl-2 and miR-25 were significantly down-regulated .Compared with model group , the cell viability was increased signifi-cantly in edaravone group .The ROS level was decreased significantly .Meanwhile, the expression of Bax was down-regula-ted, while the expression of Bcl-2 and miR-25 was up-regulated with statistical significance .The caspase-3 activity of the cells incubated with 100 mmol/L glucose and miR-25 inhibitor was increased .However, no alteration of caspase-3 activity with edaravone added simultaneously was observed .CONCLUSION: Edaravone inhibits the apoptosis of SH-SY5Y cells induced by high glucose with the potential target site of miR-25.