Purpose: Repopulation is among those which causes treatment failure in cancer radiotherapy. The study focuses on a better understanding of radiation-induced repopulation for cancer cells.Materials and Methods: It was measured by BrdUrd/DNA flow cytometric analysis that the kinetic changes after various radiation dose-time effects on human nasophayngeal carcinoma cells. The surviving fraction of the clonogenic cells was also measured by limiting dilution method.Results: (1) the shortening of Tpot of overall cell population after 0～8 Gy of irradiation was closely related to the decrease of the clonogenic cells;(2) an increased DNA synthesis was suggested as a result of the increase of radiation dose and (3) in vitro ,the proliferative status of the clonogenic cells was as same as that of overall cell population 48 hours after irradiation.Conclusion: (1) the accelerated repopulation of CNE cells after irradiation is related to their self-regulation;and (2) Tpot may be used as a predictive value in clinical radiotherapy.

Objective:To introduce an economical and pragmatic method of establishing oxygen-glucose deprivation model for hippocampal slice of neonatal rats in vitro. Methods: Hippocampal slices(400?m) from 8~10-day-old rats were transferred into an incubator with insert of Millicell membrance and incubated for 7d,14d,20d and 30d respectively.Then they were observed under convened microscope to evaluate their growing state,and detected their ability to absorb propidiumiodide(PI) under fluorescent microscope after they had been stained by PI , The state of the hippocampal slices was observed at different time after aerating Nitrogen gas. Results:(1)No PI labelled cells were found after 7d and 14d.However,15% PI positive cells were found after 30d.(2)The longer in Nitrogen gas ,the more apoptosis cells.(3)Intensive fluorescence was observed in the CA1, CA3 and DG areas of the hippocampal slice after aerating Nitrogen gas for 1.5h.Conclusion:The hippocampal slice can be alive for about 30 days.Stable oxygen-glucose deprivation model of the hippocampal slice in vitro was successfully established after aerating Nitrogen gas for 1.5h.

Objective:To introduce an economical and pragmatic method of establishing oxygen-glucose deprivation model of hippocampal slice in neonatal rats in vitro,Methods:Hippocampal slices(400?m)from 8~10-day-old rats are transferred into an incubator with insert of Millicell membrance and incubated for 7 d,14 d,20 d and 30 d,respectively.The growing state of the hippocampal slices were observed under the convened microscope,and its ability to absorb propidiumiodide (PI) was detected under fluorescent microscope after the hippocampal slice was stained by PI,and the state of the hippocampal slices at different time after aerating Nitrogen gas was observed. Results: ① No PI labelled cells were found after 7 d and 14 d. However,15% PI positive cells were found after 30 d. ②The longer in Nitrogen gas,the more apoptosis cells. ③Intensive fluorescence was observed in the CA1,CA3 and DG areas of the hippocampal slice after aerating Nitrogen gas for 1.5h. Conclusion:The hippocampal slice can be alive for about 30 days.Stable oxygen-glucose deprivation model of the hippocampl slice in vitro was successfully established after aerating Nitrogen gas for 1.5 h.

Lysyal oxidase (LOX),a cooper dependent monoamine oxidase,can stabilize the extracellular matrix and is closely related to the tumor cell differentiation,vicious transformation and invasiveness.LOX is secreted into extracellular,and it is resolved into a small fragment by sol protease,which is lysyal oxidase propeptide (LOX-PP).According to research,LOX-PP has its own structure and physiological functions,which can inhibit the growth of tumor cells.LOX-PP also plays a significant role in the generation and progression of tumor,which is likely to be a new therapeutic target.

AIM:To observe the effects of edaravone on high glucose-induced apoptosis of SH-SY5Y cells and its potential mechanism .METHODS:The SH-SY5Y cells were cultured in the DMEM medium with 100 mmol/L glucose and 100μmol/L edaravone for 24 h.The viability of the SH-SY5Y cells was detected by MTT assay .The levels of ROS in the cells were determined by DCFH-DA fluorescent probing .The apoptotic rates of the cells were analyzed by flow cytome-try.The protein expression of Bax and Bcl-2 in the cells were detected by Western blot .The expression levels of micro-RNA-25 (miR-25) were determined by real-time PCR.To further clarify the target sites of edaravone on inhibiting apopto-sis induced by high glucose , miR-25 inhibitor was applied to the SH-SY5Y cells and the activity of caspase-3 was meas-ured.RESULTS:Compared with control group , the cell viability was decreased significantly in model group , and the ROS level was increased significantly .The protein expression of Bax was up-regulated significantly , while the expression levels of Bcl-2 and miR-25 were significantly down-regulated .Compared with model group , the cell viability was increased signifi-cantly in edaravone group .The ROS level was decreased significantly .Meanwhile, the expression of Bax was down-regula-ted, while the expression of Bcl-2 and miR-25 was up-regulated with statistical significance .The caspase-3 activity of the cells incubated with 100 mmol/L glucose and miR-25 inhibitor was increased .However, no alteration of caspase-3 activity with edaravone added simultaneously was observed .CONCLUSION: Edaravone inhibits the apoptosis of SH-SY5Y cells induced by high glucose with the potential target site of miR-25.

Objective To compare the efficacy and incidence of complications of stenting alone and intratumoral injection of recombinant human adenovirus p53 combined with stent implantation in the treatment of advanced esophageal cancer patients.Methods 2 4 cases with advanced esophageal cancer,who could only eat semi liquid,liquid food or could not eat,were randomly divided into control group(A group) and treatment group (B group).12 cases in A group were treated with esophageal stent implantation,12 cases in B group were given endoscopic intratumor injection of recombinant human adenovirus p53 combined with food tract stent implantation.Results The rate of improvement of dysphasia had no significant difference in survival period:the treatment group survival time of 3.2-38 months,mean (27.0 ± 2.3) months,while the control group survival time of 2.8-27months,an average of (14.7 ± 8.0) months,the difference between the two groups was significant (t =3.521,P ＜ 0.05).The incidence rate of restenosis in treatrnent group was 8.3％,that in control group was 41.7％.There was significant difference (x2 =7.532,P ＜0.05).Conclusion Endoscopic intratumor injection of recombinant human adenovirus p53 combined with esophageal stent implantation in the treatment of advanced esophageal carcinoma can prolong the survival time of patients with esophageal stent implantation,and to reduce the incidence of restenosis.

Aiming at the technical characteristics and transforming difficulties for patents in medical health industry,this paper makes proposition on establishing a service system for patent transformation in healthcare system and accelerating the transformation and clinical application of medical technologies through the enhancement of technical service platform construction,strengthening of professional staff capabilities,and the improvement of patent values assessment.

This study aimed to explore clinical features of multiple myeloma in patients over 80 years old. Methods: We retrospectively analyzed 11 cases of multiple myeloma over 80 years old, which were diagnosed from 2000 to 2013 in our hospital. Results:The mean age was 83.5 years. All patients had more than two complicated diseases, and the performance status was poor in the majority of the patients. Ten cases received individualized treatments. The results showed that three patients had progressed, one patient achieved complete remission, three patients achieved partial remission, and four patients achieved minor remission. Median survival time was 28 months. The causes of death included six progressed myelomas, three pneumonias, and two acute myocardial infarctions. Conclusion: Patients aged 80 years old with myeloma are often at a late stage. Treatment should be individualized based on patient status. Survival time was evidently prolonged in very elderly patients with MM after individualized treatments, particularly in patients in stagesⅠandⅡ.

Objective: To observe the effect of puncturing Juliao (GB 29) in treating carpal joint sprain. Methods: 99 cases were randomly divided into 3 groups, treatment, control 1 and control 2 groups, 33 cases for each group. Cases in treatment group were treated by acupuncture on Juliao (GB 29); patients in control 1 group were treated by acupuncture at Sanyinjiao (SP 6), and patients in control 2 group were treated by local application. The clinical effect was compared among three groups after the treatment. Results: The effective rate in the treatment group is 96.9%, which is over and above 84.8% in control 1 group and 75.8% in control 2 group (both P＜0.05). Conclusion: Acupuncture at Juliao (GB 29) has a precise curative effect in treating sprain of carpal joint. It is more effective than acupuncture at Sanyinjiao (SP 6) and local application.

Carcinoma ; classification ; pathology ; Consensus ; Humans ; Thymoma ; classification ; pathology ; Thymus Neoplasms ; classification ; pathology ; World Health Organization