OBJECTIVE:To study the preparation of fluconazole eyedrops as well as its quality control.METHODS:The content of fluconazole in eyedrops was determined with HPLC.The quality control of eyedrops has done in accordance with ChP.RESULTS:The average recovery was100.4%,RSD=0.54%(n=5).CONCLUSION:The formula of this preparation is reasonable with simple and convenient technology.Moreover,its quality control is easy to handle.

This paper reviewed the existing literatures of executive function in obsessive -compulsive disorder (OCD) patients, and critically examined four neurocognitive tests in the recent ten years' studies, including stroop task, Wisconsin Card Sorting Test(WCST),London tower, and Trail-making test. In addition, we discuss the problems occurred in the past experiments, and put forward suggestions for further studies.

Objective: To develop the Chinese mood adjective check list (CMACL) and study the validation of this scale. Methods: The CMACL was filled by 1010 subjects aged 21.0 ? 7.4, 507 males and 464 females (39 lost gender information), which were randomly split into two samples after balancing the gender for exploratory factor analysis (EFA) and confirmatory factor analysis (CFA). 232 middle school students divided into five groups according to their grades were engaged in the test-retest study at the first to fourth and sixth weeks. 34 volunteers finished the CMACL and self-rating depression scale (SDS) and 30 college students joined the validation study to test the correlation between the CMACL and state trait anxiety inventory. Results: A four-factor model was found by EFA and confirmed by CFA, which has the following dimensions: Fidget(F)?Happy and excited(HE), Pain and sad(PS),Angry and hate(AH). 30 items were also left in the CMACL after the modification(GFI=0.902,AGFI=0.883,NFI=0.884,TLI=0.931,CFI=0.937,RMSEA=0.046). Reliabilities of the four factors are higher than 0.80. Validation studies showed: (1) SDS total score is significantly positive-correlated with F (P

Objective: to develop the peptic ulcer (PU) symptoms self rating scale (PUSRS) and study the validation of this scale. Methods: The PUSRS was filled by 151 PU patients, 118 males and 30 females (3 lost gender information, aged 38.61?14.99), which were recruited from 18 gastroenterology departments. 30 PU patients were engaged in the test-retest study. 40 PU patients and 30 gastritis patients joined the discriminate validation study. Results: Two factors, pain factor (PF) and dyspepsia factor(DF), were found by exploratory factor analysis. Reliabilities of the two factors are 0.786 (PF) and 0.753(DF). Validation studies showed that PU patients have significantly higher PUSRS total score (P

The selective toxicity of the mitochondria-specific cationic fluorescent dye rhodamine 123 (Rh-123) on HeLa cells in culture was studied. In this report, we demonstrate that with continuous exposure, Rh-123 markedly inhibited the growth of HeLa cells but had little effect on normal human kidney fibroblasts. With continuously exposed to Rh-123, the growth rate, colony forming ability, and mitotic index of HeLa cells were decreased. The mechanism of toxicity of Rh-123 on HeLa ceils was investigated by EM and enzyme cytochemistry stain. The mitochondria of carcinoma cells were the main targets for the inhibitory action of Rh-123, since they selectively accumulated the dye. At the dosage of Rh-123 which was toxic to HeLa cells, the structure and function of mitochondria were disrupted, as the mitochondriarelated enzymes, i. e., ATPase, LDH, and SDH were inhibited. The possible mechanism of the action of Rh-123 on HeLa cells is briefly discussed.

Objective To study the curative effect of major aphthous ulcer using povidone-iodine,H_2O_2 asso- ciated with levamisole liniment.Methods The RAU patients were divided into 2 groups randomly.The patients of curative group were instructed to use povidone-iodine,H_2O_2 and levamisole liniment;The control group were in- structed to take metronidazole,antibiotics,compound vitamin B and vitamin C.Then the patients were observed peri- odically.Results The success rate of curative group was 85 % and the control group was 55 %.The difference had statistical significance(P＜0.01).Conclusion Treatment of major aphthous ulcer using povidone-iodine,H_2O_2 and levamisole liniment is effective.

Objective To analyze the expression levels of matrix metallo proteinase-2 (MMP-2) and matrix metallo proteinase-9 (MMP-9) in serum of patients with esophageal cancer,and to analyze the interrelations among MMP-2,MMP-9 and clinicopathologic characteristics of esophageal cancer.Methods The levels of serum MMP-2,MMP-9 from 60 patients with esophageal cancer were detected by enzyme linked immunosorbent assay.The interrelations among MMP-2,MMP-9 and clinicopathologic characteristics of esophageal cancer including age,gender,histological type,whether or not lymph nodes metastasis and TNM staging were analyzed.Results The levels of MMP-2 and MMP-9 in control group were 61.31 ng/ml and 87.67 ng/ml,the levels of MMP-2 and MMP-9 in patients with esophageal cancer were 121.66 ng/ml and 169.73 ng/ml.The levels of MMP-2 and MMP-9 were significantly higher in patients with esophageal cancer than that of general population (t =3.015,P =0.007; t =2.037,P =0.04).The level of MMP-2 was significantly associated with whether or not lymph node metastasis (t =2.150,P =0.04) and clinical staging (t =2.186,P=0.03).The level of MMP-9 was significantly associated with whether or not lymph node metastasis (t =2.390,P =0.02) and clinical staging (t =2.149,P =0.03).The levels of MMP-2 and MMP-9 have no reference to age,gender and histological type (all P＞0.05).Conclusion MMP-2 and MMP-9 may correlate with tumor invasion and metastasis in patients with esophageal cancer.

Objective:To detect the expression of cyclin B 1 in human ameloblastoma(AB). Methods:Expression of cyclin B1 was detected in 73 cases of AB (32 primary, 33 recurrent and 8 malignant cases), 19 of odontogenic keratocyst (OKC) and 7 of normal oral mucosa by streptovidin-biotin method. Results:The ratio of positive nuclear expression of cyclin B 1 in normal oral mucosa, OKC and AB was 28.6%(2/7), 36.8%(7/19) and 63.0%(46/73) respectively (P

Objective: To develop the Padua Inventory(PI) for the use of Chinese college students;explore the structure and examine the sex difference in non-clinical sample in China.Methods: The PI was filled by 1300 college subjects,including 680 males and 542 females(78 gender missing),aged 19.6?2.2,for exploratory factor analysis(EFA).The second sample has 30 college students from Peking University for test-retest study.Results: The results of EFA has four factors: impaired control over mental activities/doubting,worries of losing control over motor activities,contamination,and checking.Reliabilities of four factors are 0.94,0.85,0.86,0.83 for each and 0.96 for the total scores.The test-retest correlations of the four factors are 0.77,0.87,0.85,0.84 for each.Females scored significantly higher in factor 3(P=.000) while males scoring significantly higher in factor 4(P=.017).Conclusion: The results suggest that PI has eligible psychometric reliability,but needs further study for the validity in China.

Objective:To investigate the expressions of epidermal growth factor receptor(EGFR) and C-erbB-2 in two salivary adenoid cystic carcinoma cell lines with different metastatic potential. Methods:Salivary adenoid cystic carcinoma SACC-83 cell line and its lung metastatic cell line SACC-LM were cultured in vitro, their cytoplasm and membrane samples were extracted, and the expressions of EGFR and C-erbB-2 were detected by Western blot, the results were quantitatively analyzed by FluorChem V2.0 software.Results:EGFR expression level in cell membrane was higher than that in cytoplasm(P0.05) in both cell lines.Conclusion:Higher EGFR expression in cytoplasm and higher C-erbB-2 expression may be related to the metastatic potential of SACC-LM cells.