OBJECTIVE: To study the preventive and therapeutic effect of Weitong injection(WTI) on the postoperative intestinal adhesion of rats.METHODS: Forty SD rats were randomly divided into five groups: blank control group,model control group,Dexamethasone group and high-dose WTI group and low-dose WTI group.Postoperative intestinal adhesion model was established in all the rats except those in the blank control group.On the operative day,the blank control group and the model control group received Normal saline(1 mL?kg-1);the Dexamethasone group received Dexamethasone(5 mg?kg-1),and WTI group received WTI(4 mg?kg-1 and 2 mg?kg-1,respectively) all via vena caudalis for 7 consecutive days.On day 8,laparotomy was performed on the rats for the evaluation of the degree of intestinal adhesion and the tissue of cecum was collected for the determination of the content of hydroxyproline(Hyp) and the pathological section test.RESULTS: The degree of postoperative intestinal adhesion of rats was attenuated by WTI(either high dose or low dose) or Dexamethasone.Furthermore,WTI significantly reduced the Hyp concentration in cecum tissue of rats with intestinal adhesion(P

Objective To explore the risk factors analysis of patients with hemorrhagic stroke in Zhongshan district of Dalian city in order to providing the Corresponding intervention measures.Methods From Oct 2010 to Aug 2013,183 cases of patients with hemorrhagic stroke(age ＞ 60 years old) and 158 cases healthy physical people in Zhongshan Hospital Affiliated to Dalian University were served as observation group and control group.Clinical information and life habit of two groups were recorded,the height and weight were measured,body mess index (BMI) was calculated,blood lipid level and plasma fiber protein level were detected.Results The rate of male,age ＞70 years,BMI index ≥25 kg/m2,smoking,history of hypertension,TG ≥ 1.7 mmol/L,TC ≥ 5.7 mmol/L,fibrinogen ＜ 4.0 g/L in observation group were 68.85 ％ (126/183),65.03％ (119/183),61.75％ (113/183),77.05％ (141/183),32.24％ (59/183),51.37％ (94/183),62.30％ (114/183),68.31％ (125/183) respectively,higher than those of control group (51.90％ (82/158),44.94％ (71/158),39.87％ (63/158),17.72％ (28/158),18.99％ (30/158),29.11％ (46/158),36.08％ (57/158),82.91％ (131/158),the differences were statistically significant (x2 =8.725,27.167,12.742,95.064,5.825,14.056,18.478,7.314,P ＜ 0.05).However,there were no significant differences in terms of diabetes history,drinking,high density lipoprotein-cholesterol level and low density lipoprotein-cholesterol between two groups.Conclusion Hemorrhagic stroke is closely related to many factors.Sex,age ＞ 70 years,smoking,obesity,history of hypertension,high cholesterin (TC ≥ 5.7 mmol/L) are independent risk factors of patients with hemorrhagic stroke in elderly.Through the control target population's blood pressure and blood lipids,rational diet,appropriate exercise,quit smoking and alcohol,adjust the good point of view,we can prevent the occurrence old hemorrhagic stroke.

After the reading behaviors ( reading altitude, reading frequency, reading habits and reading types) of 141 students in medical school were investigated and analyzed, the efficiencies of literature retrieval teaching in classroom were summarized, and the future direction was proposed.

Objective To recognize normal sinus rhythm (NSR), ventricular tachycardia (VT) and ventricular fibrillation (VF) from each other accurately and promptly. Methods A nonlinear descriptor based on multi-scale analysis, Hurst index, as a feature to quantify the nonlinear dynamics behavior of the ECG signal, was quoted in this paper. Results The nonlinear technique, Hurst index was examined and evaluated with ECG signals extracted from MIT-BIH Arrhythmia Database, MIT-BIH Malignant Ventricular Ectopy Database, and CU Ventricular Tachyarrhythmia Database under a specific moving-window length. The experiment showed good performance of this nonlinear descriptor. When the window length was 5 s long, the recognition accuracy for each of NSR, VT and VF was 100%. Besides, the computing speed was much faster than the speed obtained with a traditional non-linear technique, the complexity measure algorithm. Conclusion The Hurst index has a strong potential for life-threatening ventricular arrhythmia recognition in clinical applications.

OBJECTIVE:To reduce dispensing errors in Pharmacy intravenous admixture service(PIVAS),improve compound-ing quality of transfusion and guarantee the safety and rationality of clinic transfusion. METHODS:The case tracking method was applied to analyze each link of the work in PIVAS to find the reasons of configuration errors and control risk;and then the system tracking method was applied to analyze the hidden safety risk and the crack of quality from the inside of the system and work flow. Countermeasures and supporting continuous improvement were established to evaluate the effect of management before and after 6 months. RESULTS:Tracer methodology(TM)was adopted to find out the weakness in the key link and work flow of the cause of the configuration errors,the relative work pattern and work environment was improved,the relative system was formulated and completed,strength of rewards and punishment was increased. Through the implementation of the countermeasures,the number of dispensing errors was reduced from 81 to 38,and the error rate was reduced from 0.035% to 0.011%(P<0.01). CONCLU-SIONS:TM can be adopted in the key link and work flow of the cause of the configuration errors,aim at formulate and strictly carry out relative countermeasures to reduce errors in PIVAS,and promote the safety of drug use.

Objective To investigate the clinical effectiveness of pelvic floor reconstruction surgery with Prolift system and rou‐tine transvaginal surgery in treating pelvic organ prolapse (POP) .Methods We analyzed an retrospective study of 64 patients with POP patients from June 2009 to March 2011 .All patients were divided into two groups ,32 cases of the reconstruction group were treated with pelvic floor reconstruction surgery with Prolift system ,as reconstruction group ,the other 32 cases in routine treatment group with routine transvaginal surgery ,which inclouded transvaginal hysterectomy ,repair of anterior and posterior vaginal wall ,as routine treatment group ,the pre operative ,peri operative data and follow up results were compared between the two groups and to evaluate the outcomes of the two groups after surgery .Results The age ,body mass index ,menopause age ,pregnant times and the degree of uterus prolapse were no significant difference between the two groups (P>0 .05) .The operation of all the patients were successful .No patient were infected after surgery ,no vascular injury ,or urinary system injury ,or rectal injury occurred .Reconstruc‐tion group showed more significant in the amounts of blood loss ,average operation time ,anal exsufflation time ,mean of highest postoperative body temperature ,the urinary canal indwelling time and the postoperative hospitalization time compared with routine treatment group(P<0 .05) .The follow up rate was 93 .8% (30/32)in reconstruction group ,1 patient (3 .1% )experienced recur‐rence ,1 patient(3 .1% )experienced mesh erosion ,1 patient(3 .1% ) experienced new urinary tract symptoms ,of 19 patients who were sexually active ,1 patient suffered from algopareunia and 2 from sexual intercourse discomfort respectively .The follow up rate was 90 .6% (29/32)in routine treatment group ,4 patients(12 .5% )experienced new urinary tract symptoms ,8 patients(25% )expe‐rienced recurrence ,of 16 patients who were sexually active ,3 patients suffered from algopareunia and 5 from sexual intercourse dis‐comfort .The objective cure rates at 3 and 6 months after operation was no significant difference between the two groups (P>0 .05) ,but the objective cure rate at 12 months after operation was significantly different between the two groups (P<0 .05) .The objective curative rate in reconstruction group was significantly higher than that in routine treatment group .Conclusion Pelvic floor reconstruction surgery with Prolift system is a safe and effective methods of minimally invasive surgery ,patients had better regain integrity of anatomical structure and functions of pelvic floor in short term for POP when compared with routine transvaginal sur‐gery ,but its long term study is still needed .

Objective To explore the influence of Lipo PGE1 on endothelin (ET-1) and interleukin (IL-6) in exhaled breath condensate(EBC) of patients with chronic obstructive pulmonary disease (COPD) and pulmonary hypertension(PH) and its clinical significance.Methods A total of 40 cases of patients with COPD and PH were randomly divided into thecontrol group and the treatment group,20 cases in each group.The control group was administered with the conventional treatments such as anti-infection,bronchodilator,antiasthma,expectorant and oxygen therapy;the treatment group was administered with Lipo PGE1 (10 μg/d,iv,for 10 days) besides the conventional treatments.ET-1 and IL-6 in EBC,pulmonary artery systolic pressure(PASP),arterial blood PaO2 and PaCO2,lung function FEV1/FVC,FEV1%pred in both groups were assayedbefore and after the treatment.Results There were no statistical difference between the two groups in the ET-1 and IL-6 in EBC,PASP,arterial blood PaO2 and PaCO2,FEV1/FVC,FEV1% pred before the treatment.After the treatment,the ET-1,IL-6 in EBC,PASP and arterial blood PaCO2 of the treatment group were lower than thoseof the control group (P<0.05);the arterial blood PaO2,FEV1/FVC,FEV1% pred of the treatment group were higher than those of the control group (P<0.05);The levels of ET-1 and IL-6 in EBC wereboth positivelycorrelated with PASP in the two groups.Conclusion Lipo PGE1 can reduce the levels of ET-1 and IL-6 in EBC of patients with COPD and PH.ET-1 and IL-6 may become a curative effect judgment index of COPD and PH,which has a certain clinical significance.

Objective To explore the clinical features and long-term prognosis of juvenile idiopathic arthritis (JIA)in children, as well as particular quality of life associated with the clinical features and therapeutic options. Methods Seventy patients from August 1997 to August 2007 were retrospectively reviewed and survey was conducted using a questionnaire indicating quality of life(CHAQ questionnaires and CHQ questionnaire, adjusted appropriately) either by phone,letter or out-patient follow-up approach.The manner of correlation and logistic regression was used to analyze quality of life associated with the clinical features and therapeutic options. Results (l)The majority (74.3%) of patients were diagnosed as systemic onset JIA. The Cronbach Alpha coefficient of questionnaire is 0.9599, with a higher inernal consistency reliability. The scales validity also had the higher construction. Sixty nine cases with 76.8 percent of the score showed good quality of life. Quality of life in children less than 5 years old was significantly different from those more than 5-year old( P < 0.05). The rank sum test of single-use NSAIDs group and NSAIDs adding other medicine group suggested that the two groups had differences in quality of life( P = 0.026) .The correlation coefficient between the variables of time from onset to formal treatment and quality of life scores was 0.329( P < 0.05), and "walking"as the dependent variable for the regression analysis showed regression coefficient was 0.15( P = 0.016). The variable grip had the significant relationship with the "disease onset to regular treatment, the initial number of joint involvement" respectively. Conclusion Systemic oneset JIA is the most common type of JIA. Most of JIA patients have a good prognosis.The major factors which may cause the decline in the quality of life are age, duration without therapy after the disease onset,and the compliance to medication.To achieve a better prognosis,more aggressive therapy may be needed and individulized.

Objective To analyse subtyping of iscbemic stroke by the criteria of Trial of Org 10 172 in Acute Stroke Treatment (TOAST), its distribution, and relationship between TOAST subtypes, its risk factors and recurrence. Methods Clinical data of 441 patients of ischemic stroke were collected prospectively during October 1, 2006 to September 30, 2007. All the patients were classified by TOAST criteria into five major subtypes and followed-up for one year since its first episode. Dates of its recurrence and death due to recurrence were recorded. Results All the 441 patients with ischemic stroke could be subtyped etiologically according to TOAST criteria as follows: etiology undetermined in 42.3 percent, small-vessel occlusion in 30.9 percent, large-artery atherosclerosis in 17.3 percent, cardioembolism in 9.3 percent, and others in 0.2 percent, without statistically significant difference in its gender and age distributions. Recurrence rate of iscbemic stroke in one year since its first episode for varied subtypes was 33.3 percent for cardioembolism, 14. 6 percent for etiology undetermined, 13.7 percent for large-artery atherosclerosis and 6.9 percent for small-vessel occlusion, respectively. There was no significant difference in risk factors for varied subtypes of iscbemic stroke, including history of smoking, hypertension, diabetes, cardiac disease, and serum levels of total cholesterol, low-density lipoprotein cholesterol, fihrinogen and glucose. Conclusions Varied subtypes of iscbemic stroke had different risk factors and recurrence rate by TOAST criteria, which can be used as an etiologic classification for its secondary prevention.

Aim Background tumor necrosis factor-alpha (TNF-?) is produced by monocyte-macrophages and adipocytes. It may provide the link between inflammation and atherosclerosis. The aim of this study was to evaluate the effect of fenofibrate on serum TNF-? concentration and TNF-? secretion by adipocytes from hypercholesterolemia rabbits.Methods Ten male New Zealand white rabbits were fed with high-cholesterol diet for 8 weeks, and were randomly divided into two groups: ①high cholesterol group: maintained cholesterol diet for 4 wks; ② fenofibrate group: the same cholesterol diet supplemented with fenofibrate (30 mg?kg?d -1) for 4 weeks. And control group was fed with normal diet for 12 weeks. Subcutaneous adipose was collected for adipocytes culture. TNF-? concentrations in serum and adipocytes culture supernatant were measured by ELISA. Adipocytes were harvested for semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) studies. Results :Rabbits fed with high-cholesterol diet showed higher serum levels of total cholesterol, low density lipoprotein cholesterol than those fed with normal diet (P