Objective To reveal the trend of change incidence of hepatitis A(HAV)in Shandong province and explore dynamic analysis methods.Method Dynamic analysis was made on data of hepatitis A in Shandong province from 1990 to 2006.Results The reported incidence of hepatitis A patients dropped from 53.97/100,000 to 1.14/100,000 in Shandong province from 1990 to 2006,which showed a marked downward trend;the groups under the age of 10 dropped from 55.60% to 5.12%,the average age of incidence moved from 14.40 years old to 40.19 years old.Conclusion The incidence of hepatitis A has been changed from prevalent to sporadic in Shandong province,and there was a further downward trend;the age of the high-risk population has been shifted to older age group;the sex ratio of incidence between males and females in the population above 10 years of age had an expansion trend,the age distribution curve differences between males and females was also expanded.

Objective:To establish an in vitro model for the development of mouse spermatogenic cellsinto sperm by using the immunodefective mouse as the incubator.Methods:Tissue grafting was per-formed using testis from 1-2 days old Kun-ming mice as donor tissue and immunodefective mice as recipi-ents;the expression of TESK1 mRNA in grafts was determined by RT-PCR and the spermatogenesis fur-ther observed with histological analysis of grafts.Results:Molecular biological and histological analysesshowed that grafts post-grafting not only expressed TESK1 mRNA as in normal mouse testis,but also ex-hibited similarities in the structure of seminiferous tubules and component of spermatogenic cells,inclu-ding sperms.Conclusion:Spermatogenic cells heterotopically grafted in vitro could continuously growand complete spermatogenesis and finally develop into sperm.

OBJECTIVE: To evaluate the effect of butorphanol on postoperative catheter-related bladder discomfort(CRBD).METHODS: Sixty male cases(ASA grade Ⅰ～Ⅱ) undergoing epigastric surgery were randomly assigned to receive Butorphanol injection(n=30,trial group) or same volume of normal saline(n=30,control group) 45 min before the withdrawal of tracheal catheter.The prophylactic and analgesic effect of butorphanol on CRBD as well the adverse drug reactions such as nausea & vomiting,and dry mouth were observed.RESULTS: Both the incidence and severity of CRBD were lower in the trial group than in control group at each time point(P

Objective To optimize the solid-phase extraction method by comparison of the extraction recovery of docetaxel plas-ma samples.Methods After extracted by different solid-phase cartridges with water,acetonitrile,and different content methanol e-lution,the plasma samples were analyzed on an Phenomenex LUNA C18 column(4.6 mm×250 mm,5μm)with mehhanol:acetoni-trile:water(42∶26∶32,v/v/v)as the mobile phase.From the recovery achieved,the best solid phase cartridge was found.Results This method consists of using 60%-80% methanol for the elution.Among the three kinds of solid-phase being tested,Waters Oasis HLB cartridge was found to be the best one.Conclusion The average extraction recovery of the Waters Oasis HLB cartrid-ges was between 95%-105%,and it can be used in the analysis of docetaxel in plasma samples.

Humans ; Hypoxia-Ischemia, Brain ; diagnosis ; therapy ; Infant, Newborn ; Infant, Premature

Objective To investigate and analyze the effect of two different doses of iopamidol enhancement on hepatic CT. Methods In Lishui people's hospital from January 2014 to August 201680 patients who accept hepatic enhancement scan were randomly divided into two groups, the study group and the control group, 40 cases in each group. The control group were received liver CT scan with iopamidol as contrast agent, the concentration was 300 g/L, the dosage was 1.5mL/kg, the concentration and dosage in the study group was respectively 300g/L and 1.2mL/kg. The enhancement effect after scanning in the two groups were observed and compared. Results The imaging quality in arterial phase and in portal phase in the study group were better than those in the control group (P<0.05); The adverse reactions in the study group were more than those in the control group, the difference between the two groups was statistically significant(P<0.05). Conclusion In liver CT scanning process, iopamidol as the contrast agent, the enhancement effect of 1.5mL/kg of iopamidol is better than 1.2mL/kg of iopamidol, but the efficiency scanning effect is improved at will also increase allergies and other adverse reactions..

Asphyxia Neonatorum ; complications ; Humans ; Hypothermia, Induced ; methods ; trends ; Hypoxia-Ischemia, Brain ; diagnosis ; therapy ; Infant, Newborn ; Neurologic Examination

Multiagent chemotherapy has significantly improved survival for childhood ALL.However,the relapse rate with current therapies remains at 20% to 30%.Numerous studies have proved that the minimal residual disease(MRD)is associated with relapse rate.The major methods for the detection of MRD include polymerase chain reaction(PCR)and flow cytometry(FCM).The measurement of MRD in childhood acute lymphoblastic leukemia offers the promise of individualized,risk-stratified treatment and the prediction for post-transplant outcome.

Objective:To investigate the effect of Luteolin on the degranulation function of RBL-2H3 mast cells and its mechanism of action.Methods:DNP-BSA-IgE was used to establish the sensitized cell model in RBL-2H3 cells.Cell viability was exa mined by MTT assay at different concentrations (5,15,25 μmol/L) of Luteolin pretreated RBL-2H3 cells.The effect of Luteolin on β-hexosa minidase(β-hex) and TNF-αwas evaluated by ELISA.The change of Ca2+influx was detected by Flou-4AM calcium ion fluorescent probe.The expression of AKT and P-AKT were detected by Western blot.Results:The sensitized cell model was established successfully .Cell viability had not significantly changes stimulated by different concentrations of Luteolin ,and a significantly inhibition role showed the release of β-hex and TNF-αfrom RBL-2H3 cells.The concentration of intracellular Ca 2+ was significantly decreased.However,the Western blot results showed that the level of Phosphorylation AKT was decreased .Conclusion: Luteolin suppress RBL-2H3 cell degranulation in dose dependence ,which may via impacting the Ca2+influx and the activity of AKT.

Esophageal cancer is a highly aggressive malignant tumor of the digestive tract. Despite the continuous development of sur-gical techniques and multidisciplinary treatments, the prognosis and overall survival of esophageal cancer patients is still poor. In re-cent years, the development of new molecular targeted therapy has become well advanced. Drugs that target tumor-related signal pathways and block the transmission of downstream signals can inhibit the growth and metastasis of tumor cells. Currently, esopha-geal cancer-targeted drugs are rarely approved officially. HER-2 inhibitor represents these types of drugs because of its positive expres-sion of metastatic adenocarcinoma. However, studies on the preparation of new drugs are not extensive. In this paper, the research progress of a new targeted therapy for metastatic esophageal cancer is reviewed.