Objective To reveal the trend of change incidence of hepatitis A(HAV)in Shandong province and explore dynamic analysis methods.Method Dynamic analysis was made on data of hepatitis A in Shandong province from 1990 to 2006.Results The reported incidence of hepatitis A patients dropped from 53.97/100,000 to 1.14/100,000 in Shandong province from 1990 to 2006,which showed a marked downward trend;the groups under the age of 10 dropped from 55.60% to 5.12%,the average age of incidence moved from 14.40 years old to 40.19 years old.Conclusion The incidence of hepatitis A has been changed from prevalent to sporadic in Shandong province,and there was a further downward trend;the age of the high-risk population has been shifted to older age group;the sex ratio of incidence between males and females in the population above 10 years of age had an expansion trend,the age distribution curve differences between males and females was also expanded.

OBJECTIVE: To evaluate the effect of butorphanol on postoperative catheter-related bladder discomfort(CRBD).METHODS: Sixty male cases(ASA grade Ⅰ～Ⅱ) undergoing epigastric surgery were randomly assigned to receive Butorphanol injection(n=30,trial group) or same volume of normal saline(n=30,control group) 45 min before the withdrawal of tracheal catheter.The prophylactic and analgesic effect of butorphanol on CRBD as well the adverse drug reactions such as nausea & vomiting,and dry mouth were observed.RESULTS: Both the incidence and severity of CRBD were lower in the trial group than in control group at each time point(P

Objective:To establish an in vitro model for the development of mouse spermatogenic cellsinto sperm by using the immunodefective mouse as the incubator.Methods:Tissue grafting was per-formed using testis from 1-2 days old Kun-ming mice as donor tissue and immunodefective mice as recipi-ents;the expression of TESK1 mRNA in grafts was determined by RT-PCR and the spermatogenesis fur-ther observed with histological analysis of grafts.Results:Molecular biological and histological analysesshowed that grafts post-grafting not only expressed TESK1 mRNA as in normal mouse testis,but also ex-hibited similarities in the structure of seminiferous tubules and component of spermatogenic cells,inclu-ding sperms.Conclusion:Spermatogenic cells heterotopically grafted in vitro could continuously growand complete spermatogenesis and finally develop into sperm.

Objective To optimize the solid-phase extraction method by comparison of the extraction recovery of docetaxel plas-ma samples.Methods After extracted by different solid-phase cartridges with water,acetonitrile,and different content methanol e-lution,the plasma samples were analyzed on an Phenomenex LUNA C18 column(4.6 mm×250 mm,5μm)with mehhanol:acetoni-trile:water(42∶26∶32,v/v/v)as the mobile phase.From the recovery achieved,the best solid phase cartridge was found.Results This method consists of using 60%-80% methanol for the elution.Among the three kinds of solid-phase being tested,Waters Oasis HLB cartridge was found to be the best one.Conclusion The average extraction recovery of the Waters Oasis HLB cartrid-ges was between 95%-105%,and it can be used in the analysis of docetaxel in plasma samples.

Humans ; Hypoxia-Ischemia, Brain ; diagnosis ; therapy ; Infant, Newborn ; Infant, Premature

Asphyxia Neonatorum ; therapy ; Humans ; Infant, Newborn ; Infant, Newborn, Diseases ; Neonatology ; methods ; Resuscitation ; methods

Asphyxia Neonatorum ; complications ; Humans ; Hypothermia, Induced ; methods ; trends ; Hypoxia-Ischemia, Brain ; diagnosis ; therapy ; Infant, Newborn ; Neurologic Examination

Observing the clinical curative effect of orthopedics lotion to treat traumatic ankle arthritis, the outpatient department of orthopedics in Guang'anmen Hospital collected 60 cases who were diagnosed as traumatic ankle arthritis. The cases who already met the inclusion criteria, were randomly divided into the treatment group (30 cases) and control group (30 cases). Thirty patients in treatment group were received fumigation treatment with orthopedics lotion; 30 patients in control group were treated by intra-articular injection of sodium hyaluronate. After 5 weeks treatment, the effects on the both groups would be observed and analysed. Baird-Jackson scoring system was used to assess the overall curative effect. Visual analogue scales (VAS) was used to assess analgesic effect. BJ scores of pre-treatment and post-treatment in both the treatment group and the control group were compared, P <0O. 01, significantly different; and the BJ scores after treatment of two groups were compared by paired t-test, the t value is 1. 76, there was no significant difference between two groups. Both of the treatments were effective, but there was no significant difference in overall curative effect between the two kinds of treatment. The VAS scores after treatment of two groups were compared by paired t-test, the t value was 2. 282, and there was a significant difference between two groups (P <0. 05). The analgesic effect shown in treatment group was better than the control group. The results suggest that: orthopedics lotion is a effective way to treat traumatic ankle arthritis, and it has significant effect on analgesic.
Adult ; Ankle Injuries ; complications ; Arthritis ; complications ; therapy ; Female ; Humans ; Male ; Medicine, Chinese Traditional ; methods ; Middle Aged ; Orthopedics ; methods ; Treatment Outcome

Pancreatic ductal adenocarcinoma is the most common pancreatic neoplasm characterized by latent morbidit,poor prognosis,high mortality rate and limited choice of treatment.Quitealot studies focused on its pathogenesis,and showed molecular genetic alterations,which derived of genetic and environmental factors and played an important role in tumorigenesis.Recently,more and more findings laid particular emphasis on the changes of gene molecule and some were confirmed in vitro and in vivo.In this paper,we made a review and summarized the marked molecular changes and signalings of the four major pathways to understand their functions in PDAC.

Non-coding RNA (ncRNA) is a class of RNA not coding the protein and plays a significant role in the process of growth and development of diseases.Barrett''s esophagus (BE) is considered as the precancerous lesion of esophageal adenocarcinoma.Several studies showed that ncRNA has the potential value in diagnosis, treatment and designing of targeting drugs.This article reviewed the role of ncRNA, especially microRNA, long non-coding RNA and circular RNA in the development and carcinogenic process of BE.