OBJECTIVE:To discuss clinical pharmacists' role in clinical drug treatment. METHODS: Case study was performed retrospectively on clinical pharmacists' involving in clinical drug treatment. RESULTS & CONCLUSION: Hospital pharmacists' participating in drug treatment had achieved remarkable outcome. Clinical pharmacists have to cooperate with medical staff in clinical drug treatment so as to bring their due role into full play.

Objective To analyze the irrational prescriptions, improvement measures and improvement effect in our hospital in 2016. Methods 7200 outpatient and emergency prescriptions of our hospital in 2016 were randomly selected to study. 3600 prescriptions from January 2016 to June were selected as pre intervention group, and 3600 prescriptions from July 2016 to December were selected as post intervention group,First of all, the prescription of the pre intervention group was reviewed, according to the results of the review and literature review to determine the intervention measures. The types of prescription drugs, average amount of prescription, rate of antibiotic use and unreasonable prescription rate of two groups were observed. Results Types of prescription drugs(2.27 kinds vs. 1.93 kinds), average amount of prescription(171.74 yuan vs. 152.86 yuan), rate of antibiotic use(34.39% vs. 25.47%) and unreasonable prescription rate (9.36% vs. 2.14%)of post intervention group were significantly lower than those of pre intervention group(P<0.05). Conclusion The prescription comment and targeted intervention can reduce the incidence of irrational drug use and promote rational drug use in clinic, which has important clinical value.

Objective To study the relationship between urinary heparin and human bladder transitional cell carcinoma(BTCC) Methods Reverse polarity capillary electrophonesis(RPCE)in a phosphate buffer was used to determine the value of urinary heparin in 19 patients with BTCC and in 9 normal individuals. Results The value of urinary heparin was (2.37?1.06)?g/L in 9 normal subjects,(1.11?0.45)?g/L in 9 patients with Ta～T 1 BTCC and (0.41?0.13)?g/L in 10 patients with T 2～T 4 BTCC. Conclusions Falling of urinary heparin in BTCC plays an important role in the occurrence and invasiveness of BTCC.

Objective To observe the role of preoperative single administration of zenapax in the prevention of acute rejection after kidney transplantation, and evaluate its safety. Methods A total of 80 renal transplant recipients during the same period were divided into treatment and control groups, each group containing 40 patients. There were no difference between the two groups in treatment regime except the preoperative single administration of zenapax in the treatment group. Results Acute rejection rate was significantly lower in the treatment group(7.5%) than the control group(25%) within 6 months after kidney transplantation(P

Objective To investigate the effect and mechanism of serotonin (5-HT) and secretin(SEC) on afferent discharge of vagus in rats. Methods Spontaneous afferent discharge of subdiaphragmatic vagus nerve was recorded in urethane anesthetized rats. The effects of 5-HT, 5-HT3 receptor antagonist and 5-HT+SEC on the discharge of subdiaphragmatic vagus nerve were investigated by intravenous injection of different dosage of 5-HT(3, 10, 30 ?g/kg), Granisetron (1 mg/kg) and 5-HT+SEC. Results Intravenous 5-HT caused obvious exciting effect on spontaneous afferent discharge of subdiaphragmatic vagus nerve. The 5-HT3 receptor antagonist significantly inhibited the effects of 5-HT. 5-HT+SEC could augment the effect of 5-HT on spontaneous discharge. Conclusion The exciting effect of 5-HT on afferent discharge of subdiaphragmatic vagus nerve is possibly mediated by 5-HT3 receptor related to vagus nerve afference. Secretin can augment the exciting effect of 5-HT on spontaneous discharge of vagus afferent nerve.

Objective To explore the effects of a microbubble agent (SonoVue) on radiofrequency ablation(RFA).Methods Twenty rabbits underwent RFA with or without SonoVue on two areas per rabbit liver.SonoVue were injected in bolus intravenously in 0.1 ml/kg.The morphology of hyperechogenic area were observed by conventional ultrasonography.The size of RFA lesions and ablation time were measured.The correlation was analyzed between hyperechogenic area and ablation size.Results Microbubbles didn't change the size of RFA lesions.However,with the addition of SonoVue to ablation,the morphology of hyperechogenic area was showed more regular and stable,and hyperechogenic area and ablation size were well correlated (r =0.849,P =0.000).Besides,the ablation time was much shorter (P ＜ 0.05).Conclusions The addition of SonoVue to ablation improve the prediction of ablated areas and reduce the ablation time,therefore it might be useful to prevent the unfavorable ablation of adjacent organs and vessels.

Objective To construct the multiple antigenic peptide (MAP) gene and E. coli ex-pression system, based on the out membrane protein OmpL1, LipL21 and LipL32 from Leptospira interro-gans, and better understanding of the immunological activity of the recombinant protein. Methods Using MI3KE display and Western blot, the advantage epitopes of OmpL1, LipL21 and LipL32 were identified and used to synthesize a new gene, then its prokaryotic expression system was constructed. The expression of re-combinant protein was determined by SDS-PAGE. The immunity activity of the recombinant protein was iden-tified by Western blot and ELISA. Results The synthetic gene was effectively expressed in E. coli and mainly presented in soluble form. The expression protein could react with the antileptospirosis antibodies in rabbit and human sera, which contained different serogroups. Conclusion The recombinant MAP gene of leptospires was successfully constructedand and expressed in E. coli. The recombinant protein had a good immune activity, and could cross-reacted with antileptospirosis antibodies from different serogroups.

OBJECTIVE:To investigate the cognition of medical staff's cognition on clinical pharmaceutical care.METHODS:A total of 105 questionnaires collected from the doctors and nurses were subjected to an aggregate analysis in terms of their ultimate educational background,professional title,working lifetime,cognition on clinical pharmaceutical care etc.RESULTS & CONCLUSIONS:Doctors and nurses had positive attitude toward clinical pharmacy care and it is greatly demanded in drug use.Clinical pharmaceutical staff should keep improving their expertise level and strengthen cooperation with medical staff so as to facilitate the development of clinical pharmaceutical care.

Abstract: Molecular genetic map is a fundamental organizational tool for genomic research. However, a genetic linkage map for Bupleurum chinense DC. has not been developed. In this study, with the theory of pseudo-testcross, 96 F1 plants from an intraspecific cross of B. chinense were used as mapping populations. Twenty eight ISSR (inter-simple sequence repeat) primers and 44 SSR (simple sequence repeat) primers were used to detect the polymorphisms between the parental plants, and of them, 28 ISSRs and 14 SSRs were selected to analyze the F1 populations. The map consisted of 13 linkage groups which included 80 (72 ISSRs and 8 SSRs) loci, and covered 2 633.9 cM with an average density of 33.4 cM. All 13 linkage groups consisted of 2-31 loci ranging in length from 15.4-1295.7 cM. This map will provide a basis for studies on gene mapping, map-based cloning and maker-assisted selection of important traits in B. chinense.

Objective To evaluate the differentiation diagnostic value of acoustic radiation force impulse imaging (ARFI) technique to the benign and malignant breast lesions .Methods A total of 87 breast masses in 84 patients were selected and per‐formed the routine ultrasound ,virtual touch imaging(VTI) and virtual touch quantification(VTQ) examination .The shear wave ve‐locity was obtained .The average optical density of the tumor was obtained by processing the VTI images by using the image J soft‐ware .The pathological results served as the gold standard and the ROC curve was drawn .Finally ,the significance of ARFI was e‐valuated .Results All 87 pathologically verified breast tumors included 31 malignant lesions and 56 benign lesions .In malignant le‐sions ,there were 21 lesions with VTQ value≥3 .49(threshold value) and 10 lesions with VTQ value<3 .49 .In benign lesions ,there were 3 lesions with VTQ value≥3 .49 and 53 lesions with VTQ value<3 .49 .In malignant lesions ,there were 28 lesions with the mean optical density value≥202 .25(threshold value) and 3 lesions with the mean optical density value<202 .25 .In benign lesions , there were 6 lesions with the mean optical density value≥202 .25 and 50 lesions with the mean optical density value<202 .25 .There were statistically significant differences in the shear wave velocity measured by VTQ and average optical density measured by VTI between malignant and benign lesions(P=0 .000) .The sensitivity ,specificity ,positive predictive value ,negative predictive value ,ac‐curacy rate and Youden index of conventional ultrasound were 96 .8% ,67 .8% ,62 .5% ,97 .4% ,78 .2% ,0 .645 ,respectively ;which for ARFI were 93 .5% ,98 .2% ,96 .7% ,96 .5% ,96 .6% and 0 .917 ,respectively .Conclusion The ARFI is superior to the conven‐tional ultrasound in the specificity and accuracy rate in diagnosing breast tumor .