Over the past few decades,biomarkers have become increasingly utilized as non-invasive tools in the early diagnosis and management of various clinical conditions.In perinatal medicine,the improved survival of infants who are at high risk for adverse neurologic outcomes has increased the demand for the discovery of biomarkers in detecting and predicting the prognosis of in term and preterm newborns with neonatal brain injury.Recognization potential brain damage early is crucial because clinical feature and monitoring parameters may be of no avail and therapeutic window for pharmacological intervention is very limited.Therefore,the measurement of biochemical markers of brain damage is eagerly awaited in clinical practice to detect high risk infants.These biomarkers could allow clinicians to intervene at the early stages of disease,and to monitor the efficacy of those interventions.None,however,were studied extensively enough to warrant routine clinical use.The present paper is aimed at investigating the role of the main biomarkers such as activing A,S100B,glial fibrillary acidic protein,adrenomedullin,neuron-specific enolase,interleukin-6,hemeoxygenase-1,transforming growth factor β,uric acid,nucleated red blood cells,currently studied in infants with neonatal brain injury.

Objective:To construct an eukaryotic expression vector harboring human papillomavirus 16E7(HPV16E7),and to study the effect of HPV16E7 on the biological character of human laryngeal carcinoma cell line HEp-2.Methods: The full sequence of human papillomavirus 16E7 gene was amplified from the plasmid of pET28a-HPV16E7 and was then cloned into pcDNA3.1/myc-His(-)B.The new plasmid,pcDNA3.1/myc-His-HPV16E7,was identified by sequencing and restriction digestion and was introduced into the laryngeal carcinoma cell line HEp-2 by electroporation;the morphology of the transfected cells was observed by the phase contrast microscope and the cell cycle was studied by flow cytometry.Western blot analysis was used to detect the expression of HPV16E7 proteins.Results: The plasmid,pcDNA3.1/myc-His-HPV16E7,was successfully constructed and was introduced into HEp-2 cells.The transfected cells proliferated actively with a prolonged S phase,and HPV16E7 proteins were detected in them.Conclusion: HPV16E7 can increase the tumorigenetic activity of HEp-2 cells.The construction of recombinant pcDNA3.1/myc-His-HPV16E7 paves a way for further study of laryngeal carcinoma.

It's analyzed why AIDS can't be controlled and prevented efficiently in China in this paper. Some prposals are also put forward.

OBJECTIVE:To provide references for the standardization of the management and use of narcotics drug(ND)and psychotropic substances(PS) in medical institutions. METHODS: The problem existing in the management and use of ND and PS in 102 municipal medical institutions sampled from three counties and five districts of Lanzhou were analyzed from aspects of supervision & administration agency for hygiene, medical institution, physicians and pharmacists. Meanwhile, suggestions for the solution of the problem were put forward. RESULTS & CONCLUSIONS: The problem existing in the management and use of ND and PS manifested as lack of training and examination for physicians and pharmacists in medical institutions, the concerned personnel’s inadequate understanding of regulations and old-fashioned expertise, inappropriate management, conservative in clinical medication, and irrational in the structure of drug use much as in the private medical institutions and township health centers. Measures should be taken to tackle the problem from the origin so as to standardize the use and management of ND and PS in medical institutions.

Objective To discuss dyadic fallopian tube of supermini underbelly cut windowing transplanting tiny simple skill wound method.Methods Choose 25 example mesenchymes headquarters or the gorge headquarter blocks nature barrenness(8 examples)and mesenchyme headquarter or gorge headquarter ectopic pregnancy(17 examples),adopt supermini underbelly 2.5 ~ 3.5 cm cuts to do dyadic fallopian tube of windowing transplant a skill,and transplant skill 15 examples action with the tradition fallopian tube contrasting.Results 25 example patients transplant 29 strip fallopian tube together,operation time shares 68.3 ?22.9 minutes,operation bleeding very few 35.8 +/-28.6 average mL,the skill queen is in hospital time 5.4 +/-0.8 days,no complication happens.Skill last 25 routine fallopian tube exchange the liquid experiment,17(68.0%)examples smooths,7(28.0%)examples exchanges but no free,1(4.0%)regular clausura.Conclusion That the supermini cut of underbelly and the dyadic fallopian tube of windowing transplanting skill is simple and microinvasive,which makes it worth extensive appeication.

The validity of moral education is the emphases and difficulty that current research and academic studies focus on.Taken the concept of validity as the basic point,this article analyzes the deep-seated reason why the medical ethics education loses its validity and discusses the rules to realize the validity of the medical ethics education from the aspects that the medical ethics education should return to real life,adapt to psychology laws and penetrate through the clinical stage.

Objective To make the detection results of the different automated hematology analyzers in laboratory to be reliable and comparable.Methods With the Beckman Coulter LH750 after calibration as the reference instrument,the fresh whole blood was extracted from the patient and divided into 3 equal parts after mixing evenly.The first part was determined the target value in the LH750 instrument;the second part was used to calibrate the HMX and ABX PENTRA 60 instruments and the third part served as a calibration validation.After calibration,the fresh whole blood of patient was performed the detection for conducting the accuracy evaluation.Results After calibration,the detection results of RBC,HGB,WBC,HCT,MCV and PLT by each instrument were largely improved and the error of the results was less than 1/3 of CLIA′88 requirements.Conclusion Adopting fresh whole blood for calibrating the hematology analyzer is feasible.

Objective To explore the effect of positive acceleration (+Gz) on immune barrier of intestinal mucosa in rats. Methods Thirty two male SD rats were randomly divided into 4 groups (8 each): Group A (control group), Group B (+5Gz group), Group C (+10Gz group) and Group D (repeated exposure group). The animal centrifuge was used to simulate the exposure of acceleration. Group A was no disposed. +5Gz group and +10Gz group were subjected to centrifugal force of +5Gz and +10Gz respectively for 5min; repeated exposure group was continuously exposed to 1.5min under +5Gz value, 2min under +10Gz value and 1.5min under +5Gz. All groups were exposed to the respective acceleration once daily for 5 days. The damage of intestinal mucosa was observed by light microscopy after the experiment was finished, and the content of sIgA in intestinal mucosa was detected by ELISA. Results Except for group A, intestinal mucosal injury was observed in the other three groups. Group D was shown as the most serious one, followed by group C and group B. Compared with group A, the level of sIgA was significantly lower in other three groups (P<0.05). The level of sIgA in group C was significantly lower than that in group B (P<0.05) and higher than that in group D (P<0.05). Conclusion +Gz exposure can result in intestinal injury and weaken the function of immune barrier of intestinal mucosa in rats.

Objective To study the image quality control system to ensure that equipment meet clinical needs.Methods It was scanning the Catphan504 phantom with models of high quality head,standard dose head and pelvis,we could get the results of CT numbers linearity,uniformity,spatial resolution,contrast resolution.Using T test to compare different scanning technique results.Results The standard dose head scanning technique was better than the pelvis scanning technique in CT numbers linearity test,and gets the best result in uniformity test.The result of CT numbers uniformity was higher in the standard dose head scanning than the high quality head and the pelvis scanning (9.7 ±3.9 vs.17.9 ±5.3,P =0.00 and 9.5 ± 4.0 vs.31.1 ± 5.7,P =0.00).The result of contrast resolution was higher in the pelvis scanning than the high quality head and the pelvis scanning (5.6 ± 0.1 vs.1.3 ± 0.5,P =0.00 and 6.0 ± 1.0 vs.1.3 ± 0.5,P =0.00).The result of spatial linear distance was very accurate,the range was 4.98 -5.06 cm.Conclusions The results of spatial linearity test are stable and accuracy,but CT numbers linearity and uniformity test are affected by the scanning technique significantly for device.To spatial resolution test and contrast resolution test,we need to set the standard and tolerance according to each linear accelerator specialty.

Objective The purpose of this study is to compare the most comfortable levels and neural re-sponse imaging threshold between children of cochlear implants with large vestibular aqueduct syndrome and normal inner ears. Methods Thirty-eight implanted children participated in this study and were divided into two groups. Group A consisted of 32 patients with normal inner ears and group B 6 patients with radiographically proven large vestibular aqueduct syndrome. All of the patients were the recipients of the cochlear implants by Advanced Bionics. The initial time for the first programming session was approximately one month after surgery. The psychophysical tests included M- level tests. Programming techniques used in the test were suitable for the age of patients. Sound-Wave 1.4 software was used to test tNRI. Results Using the rank sum test, M-level and tNRI of electrode 3,7,11 and 15 did not differ significantly between group A and B(P>0. 05). Conclusion The parameters of mapping are not significantly different between the implanted children with large vestibular aqueduct syndrome and normal inner ear. The management and procedures of mapping used to the subjects with normal inner ear can be applied to the children with large vestibular aqueduct syndrome.