Objective To explore the optimal formula of vascular endothelial growth factor,angiopoietin and basic fibroblast growth factor on autologous fat angiogenesis and tissue proliferation.Methods Wistar rat mesenteric fat in 9 groups trimmed into particles were added a 6μl complex solution of the three factors in the formula of 1 ∶ 0 ∶ 0,0 ∶ 1 ∶ 0,0 ∶ 0 ∶ 1,1/2 ∶ 1/2 ∶ 0,0 ∶ 1/2 ∶ 1/2,1/2 ∶ 0 ∶ 1/2,1/3 ∶ 1/3 ∶ 1/3,2/3 ∶ 1/6 ∶ 1/6,1/6 ∶ 2/3 ∶ 1/6,then injected in each rat subcutaneously.15 weeks later the transplantated fat was removed,to observe the fat cell appearance and counting the number of capillaries in the graft.Statistical genetic algorithm was used to set up a Scheffer egression model to optimize fat angiogenesis number.Results In one growth factor group fat vacuoles was uneven size;in two growth factors group,fat cell size tended to be homogeneous and in the three growth factor group,fat cell size was uniformity;the highest angiogenesis rate was 12.092,when the formula of VEGF165,Ang-1 and b-FGF was 0.380∶0.280:0.340.Conclusions Formula of VEGF165,Ang-1,bFGF at 0.380 ∶ 0.280 ∶ 0.340 could improve blood vessels density and increase implanted fat survival.

Objective To study the expression level of interleukin (IL)-22 in the peripheral blood of patients with ankylosing spondylitis and its clinical significance.Methods The mRNA level of IL-22 in the peripheral blood was measured by real-time quantitative polymerase chain reaction,and the plasma level of IL-22 was measured by enzyme linked immunosorbent assay in 30 patients with ankylosing spondylitis and 30 normal controls.Results The mRNA level of IL-22 in the peripheral blood and the plasma level of IL-22 in patients with ankylosing spondylitis were statistically higher than those in normal controls [1.82 ± 0.79 vs.1.31 ± 0.63 and (47.08 ± 17.25) ng/L vs.(30.47 ± 11.24) ng/L,P ＜ 0.05].Conclusion The level of IL-22 in the peripheral blood of patients with ankylosing spondylitis increases,and IL-22 may participate in the occurrence of ankylosing spondylitis.

Objective To observe the clinical effect of acupuncture on elderly depression. Methods A total of 63 patients were designated into a treatment group and a control group by using a random number table. Acupuncture on Ge-shu point and Shu-points of Five-Zangs were administrated for the treatment group, and Fluoxetine was used for the control group at a dosage of 20 mg, once daily. The treatment of both groups lasted for 6 weeks. Results The curative effect of acupuncture was sound on relieving depression and the scores of HAMD in the treatment group exhibited a decreasing trend. There was no statically significant difference between the two groups on the total effective rate and the cure rate. Conclusion It has a good anti-depression result to needle Shu-points of Five-Zangs.

Objective The study combined multiple local flaps to repair small or medium-sized area of facial skin and soft tissue defects,and to explore the advantages of the local flap collaboration repair.Methods Following the partition repair principle and depending on the wound size and location of these defects,the appropriate local flap was selected to cover the wound.Therefore,the incision was hidden and conformed to the face natural contour or skin wrinkles.Results 77 patients were treated in this group,74 cases got phase] healing and the other 3 got phase Ⅱ healing; all were followed-up for 1 to 8 months.The repair flap and surrounding skin had good similarity in terms of color,texture and contour,the incisions were hidden and the scars were not obvious; no tumor had recurred.The face contour and visual effects were good,and the aesthetic results were satisfactory.Conclusions The combined application of several different local skin flap to repair facial trauma defects,can not only reduce the overall difficulty and risk,reduce surgical complications,but also improve the repair quality,to maximize the recovery of facial aesthetic appearance.

Objective To explore the effect of the surgical technique of jejunum fistula and conventional indwelling nasogastric tube with total gastrectomy. Methods The clinical data of 23 advanced gastric cancer patients with total gastrectomy were retrospetively analyzed. Among 23 cases, 17 received jejunum fistual and 6 received indwelling nasogastric. The time to flatus and the ratio of postoperative complications including throat ache, nausea,pneumonia, anastomotic leak were compared. Results Compared with A group, the incidence of disadvantage spit sputum, dyssomnia, throat ache and nausea in B group reduced significantly, the time to flatus was advanced in B group. And other postoperative complications had no difference between two groups. Conclusion Jejunum fistula was rational, safe and effective in patients with total gastrectomy

The medical staff's ability to apply medical statistics is deficient at present.In order to bring up eligible students and improve the teaching quality of medical statistics,the author has advanced his thoughts and opinions on teaching contents,teaching ways and examination ways.

Objective To investigate whether the bone harvested from Cloward discectomy with trephine could substitute for iliac-crest bone grafts in arthrodesis procedure. Methods We reviewed the 30 patients with cervical spondylotic myelopathy involving a single level, which had been managed with anterior trephination discectomy and arthrodesis by the bone chips harvested from within the trephine. The bone chips harvested with trephine were then trimed and implanted erectedly similar to Robinson arthrodesis procedure. All cases had been followed-up for an average of 4.75 years. The latest results were analyzed according to JOA score system and recovery rate. The fusion outcome were assessed by anteroposterior and flexion and extension lateral radiographs of the cervical spine. Results At the latest followed-up examination, clinical results were excellent in fifteen patients(50％ ), good in eleven(36.7％ ), fair in three(10％ ) and poor in one (3.3％ ). X-ray showed solid fusion in all, and no dislodgment of the grafts. However, the cervical curves had a little loss. There was no significant bone grafts subsidence. Conclusion In cervical anterior decompression the bone chips obtained in trephination could substitute for other type of bone grafts for arthrodesis. The postoperative outcomes were not affected by the loss of the cervical curves.

0.05).Conclusions Immunologic function alteration of MPP of infants is showed mainly that Th1 immunologic response is raised,Th2 immunologic response is abated,the cellular factor are changed horizontally,but the immunology index and cellular factor are recovered quickly.There are no relations between MPP of infant and PHF-AH genotype,but there is a downward trend in activity of PHF-AH in acute stage.

Objective The present study was designed to investigate the efficiency of simvastatin therapy for experimental pul‐monary hypertension (PH) in rat ,and the effects on the number and function of circulating endothelial progenitor cells (EPC) . Methods Twenty four Sprague Dawley rats were divided into 3 groups randomly :model group ,treatment group and control group , 8 rats in each group .Rats were treated with a single subcutaneous injection of monocrotaline to induce PH (PBS used as control) . The rats in the experimental group were administrated with simvastatin 3 days following subcutaneous injection of monocrotaline .In the 21st day ,the number of circulating EPC ,the right ventricle systolic pressure of rat ,pulmonary vascular structural changes and the quantity of cultured EPC were measured .At the same time ,EPC in each group were cultured in vitro ,then the ability of prolif‐eration and function were analyzed and compared .Results The number of circulating EPC in model group was decreased signifi‐cantly compared to both control and model groups (P< 0 .01) .Compared with model group ,simvastatin treatment markedly de‐creased the RVSP and the ratio of media thickness to eternal diameter (P<0 .01) ,but the ratio of vessel area to total arterial area (VA/TAA) was definitively increased(P<0 .01) .After 7 days of culture in vitro ,both the output of EPC and the ability of prolif‐eration ,conglutination and migration of EPC in treatment group were up -regulated compared with those in model group (P<0 .01) .Conclusion This study confirmed that simvastatin effectively treat experimental PH through improving quantity and func‐tion of circulating EPC .