Objective To explore the experience of nursing the patients with hypoparathyroidism.Methods Five pregnant patients with hypoparathyroidism hospitalized in our hospital during January 2009 to December 2015 were included in the study.The nursing intervention included performance of health care during pregnancy,childbirth and postpartum nursing.Results The thyroid function of the 5 patients became normal after medication and delicate care.All underwent cesarean resection and all the infants were normal in terms of thyroid function.Conclusions Pregnancy combined with hypoparathyroidism is of high risk and harmful for mothers and neonates.So they should be under management of high risk pregnancy.The prenatal,intrapartum and postpartum nursing measures should be implemented to ensure the safety of delivery.

With the rapid development of high science and technology,new teaching methods related to computer and electronic message techniques,have been applied to the recent medical education.Classical pathological teaching model has been gradually replaced by the modern way.This paper reports our teaching reformed practice by using the new technology and form the current educational theory.

0. 05). but there was a significant difference between the treatment group and the Chinese patent medicine control group (P

Objective To establish the blood glucose fluctuation diabetic rat model by subcutaneous injecting insulin. Method The male Sprague-Dawley (SD) rats were randomly assigned into two groups, normal group ( n =20) and model group ( n =40). Normal group rats were fed with normal diet, model group rats were fed with a high-sucrose-high-fat diet. After 6 weeks, a low dose of streptozotocin ( STZ ,30mg/kg) was injected to induce hyperglycemia in model group rats. And then the model group rats were ran-dom divided into two subgroups, diabetic group ( n = 20) and blood glucose fluctuation group ( n = 20). Blood glucose fluctuation group rats were induced by subcutaneous injecting insulin twice daily. All rats were executed after intervening for 3 months, metabolic cages were used to collect 24-hour urine one week before all rats were executed. Results The average blood glucose levels (MBG), the standard deviation of daily aver-age blood glucose (SDBG) and the largest amplitude of glycemic excursions (LAGE) were significant differ-ent (P<0. 05) among normal group, diabetic group and blood glucose fluctuation group. The 24 hours, pro-teinuria of diabetic group and blood glucose fluctuation group was higher than that in normal group[( 100. 38±7. 99)mg/24h vs (18. 13±1.41)mg/24h, (123. 71±11.23) mg/24h vs (18.13 ± 1.41 ) mg/24h, P <0.01] , and blood glucose fluctuation group was higher than diabetic group[(123. 71 ± 11.23 )mg/24h vs(100.38±7.99)mg/24h, P <0.05] . Conclusions The blood glucose fluctuation diabetic rats model was successfully induced by the method of subcutaneous insulin injection in diabetic rats twice daily.

Bronchopulmonary Dysplasia ; etiology ; pathology ; prevention & control ; Humans ; Infant, Newborn ; Infant, Premature ; Inflammation ; complications ; Lung ; pathology ; Respiration, Artificial ; adverse effects

Adult ; Aged ; Aged, 80 and over ; Blood Viscosity ; drug effects ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Hemorheology ; drug effects ; Humans ; Male ; Middle Aged ; Phytotherapy ; Pulmonary Heart Disease ; blood ; drug therapy

Objective To compare three methods for culture of fetal cortical neurons of SD rats and find out the suitable culture conditions of fetal cortical neurons in vitro.Methods The cortex of 16-18-day embryonic rat was used for culture in this study.Mechanical dissociation, trypsin digestion and papain digestion were applied respectively to the neuron culture.The morphological characteristics of neuronal cells at different time points were observed and neuron purity was identified by immunofluorescence staining assay.Results High purity of the fetal rat cortical neurons was successfully achieved by all the three culture methods, and each had distinct morphological characteristics at different time points.The purity of neurons was 96.28%, 95.63%and 97.34%, respectively, with no significant differences among the three groups (P>0.05).Conclusions The three culture methods are improved in our study.Stable neurons with high purity can be obtained by all the three methods respectively, and each of these methods has distinct characteristics.

Objective To analyze the clinical features and treatment and prognosis of Paget's diseases.Method 7 cases of Paget's disease of the extramammary have been encountered. Results 3 patients with groin matastases were died within 5 years, the patients without groin matastases were all surrived.Conclusions Surgery is the treatment of first choice for Paget's disease. Prognosis of patient with groin matastases is very poor.

Objective To monitor the release of amino acids of the whole retina during and after experimental glaucoma by increasing the intraocular pressure (IOP). Methods Experimental glaucoma was induced in one of the two eyes of rabbits by increasing IOP at 120 mm Hg for 45 min under infusion of saline in anterior chamber;then the pressure was released and the needle inserted into the anterior chamber was removed,this state was maintained for another 45 min.Every 15 min during the experiment 5 rabbits were killed and experimental eyes were enucleated.Aliquots (20 ?l) of the retinal extracts (see below) were mixed with ophthaldialdehyde reagent and analysed for amino acid content by the HPLC method of Wangwei, using a 150 mm?4.6 mm,5 ?m C18 column. Results A large increase in the release of glutamate,but not of the other three amino acids monitored,occurred during initial experimental ocular hypertension.It reached peak value of (111.73?17.46) 10 -5 mmol/g at 15 min of hypertension.15 min after release of intraocular pressure,again,immediately large and specific increase in the concentration of glutamate was reached to (102.96?51.91) 10 -5 mmol/g.In eyes subjected to paracentesis of anterior chamber,no difference was found between experimental eyes and controls. Conclusion These results suggest that glutamate is triggered by increasing the IOP,and it releases not only during the period of experimental ocular hypertension,but also afterwards.

[Objective]To investigate the expression and the clinical significance of macrophage migration inhibitory factor (MIF)in the serum and lung tissues of the patients with non-small cell lung cancer(NSCLC).[Methods]Eighty-eight eligible inpatients were confirmed by pathology of lung tissues ,including 66 patients with NSCLC and 22 patients with benign lung lesions. ELISA was performed to measure serum concentration of MIF of these patients ,which was compared with 30 healthy individuals. Meanwhile,immunohistochemistry(IHC)was performed to examine the expression of MIF in the lung tissues of the two groups. MIF expression level was compared between two groups and among different subgroups of NSCLC. The correlationship between serum MIF level and high expression rate in lung tissues was also analyzed. All the data were analyzed by SPSS17.0.[Results]The serum concentration of MIF in NSCLC group was significantly higher than that in healthy control(14.79 ng/mL vs 10.69 ng/mL,P=0.001), and was slightly higher but not significantly different from benign lung lesions group(14.79 ng/mL vs 13.68 ng/mL,P=0.580). The comparison among subgroups of NSCLC showed that the serum MIF level was not only correlated with the histological grade and clini?cal stage of the cancer,but also correlated with the gender and smoking history of the host. Immunohistochemistry showed that the MIF high expression rate in the lung tissues of NSCLC was markedly higher than that of benign lung lesions group(30.3%vs 4.5%, P=0.014). Among the subgroups of NSCLC,IHC showed MIF high expression rate was correlated with the histopathologic types and clinical stage of the cancer. Simultaneously ,the serum MIF level showed a positive correlation with MIF expression rate in the lung tissues in all patients and NSCLC group(P<0.05).[Conclusion]MIF was strongly related to the clinicopathological characteristics of NSCLC. It could be helpful for the diagnosis and clinical evaluation of NSCLC.