Animals ; Toxins, Biological ; chemistry ; physiology

B-Lymphocytes ; Graft vs Host Disease ; Hematopoietic Stem Cell Transplantation ; Humans

Objective To investigate the signal transduction of bFGF to regulate the colorectal carci- noma cell growth via PI3K/PKB pathway.Methods MTT assay was used to determine the suppressive effect of bFGF on the growth rate of LoVo cell;(?-~(32)P)ATP incorporation assay was used to detect the increased activity of PKB in LoVo cell;the RT-PCR technique was used to test the expression of cyclin A;Western Blot analysis was used to detect the expressions of PKB and cyclin A protein.Results When the bFGF worked on the LoVo cell at various times,it was found that the PKB activity of the LoVo cell was increased by the(?-~(32)P) ATP incorporation assay.By preprocessing by the depressor-LY294002 and bFGF,the activity of PKB was sig- nificantly reduced(P

Objective To evaluate modified Soave procedure for the treatment of Hirschsprung's disease for the neonate and infant. Methods Seventeen cases of short and sigmoid types of Hirschsprung's disease were treated by transanal modified Soave procedure. The incision was made 1cm posterioly and 2-3 cm anterioly above the dental line; the dissection progressed from the submucosa layer up to the perineal reflex into the pelvic cavity, and the rectum was pulled through. "V" shap muscular cuff was excised on the posterior wall. The proximal colon was anastomosed to the anal mucosa. Results Mean operative time was 160 min ? 45 min, mean blood loss was 45 ml ?35 ml. No enterocolitis, anastomotic leak, perianal infection and anastomotic stenosis occurred. All patients recovered with normal defecation, and no complication was observed from a follow-up of more than 4 mos. Conclusion Modified Soave procedure is safe and effective for the treatment of Hirschsprung's disease in the neonate and infant.

Adult ; Aged ; Antineoplastic Combined Chemotherapy Protocols ; administration & dosage ; adverse effects ; Carcinoma, Non-Small-Cell Lung ; drug therapy ; pathology ; Cisplatin ; administration & dosage ; adverse effects ; Deoxycytidine ; administration & dosage ; adverse effects ; analogs & derivatives ; Drug Chronotherapy ; Female ; Humans ; Leukopenia ; chemically induced ; Lung Neoplasms ; drug therapy ; pathology ; Male ; Middle Aged ; Nausea ; chemically induced ; Neoplasm Staging ; Neutropenia ; chemically induced ; Remission Induction ; Taxoids ; administration & dosage ; adverse effects

Blast Injuries ; Deafness ; etiology ; Humans

OBJECTIVE:To provide reference for standardizing dispensing and use of intravenous drug. METHODS:266 piec-es of intravenous drug instructions were collected in PIVAS of our hospital,and then the labeling information were statistically ana-lyzed,including dispensing method,diluted or dissolved solvent before preparation and storage time after preparation. RESULTS:Among 266 intravenous drug instructions,there were 45 kinds of finished product infusion varieties and 221 kinds of drugs needing to be prepared. Among these,there were only 30 pieces of drug instructions with labeling dispensing method,accounting for 13.57%;195 pieces of drug instructions stated solvent for dilution and 22 stated solvent for dissolution before preparation,account-ing for 88.24% and 19.13%;69 pieces of drug instructions stated storage time,accounting for 31.22%. CONCLUSIONS:The la-bel information of dispensing method and storage time in intravenous drug instructions is markedly insufficient or not clear. Drug ad-ministration departments are suggested to strengthen the instruction standardization management. Meanwhile,manufacturers should specify the relevant information in intravenous drug instructions.

Objective To observe the clinical efficacy of auricular point sticking plus basic nursing for constipation of excessive syndrome in acute stage of stroke.Method Ninety patients with constipation of excessive syndrome in acute stage of stroke were randomized into a treatment group and a control group, 45 cases in each group. The treatment group was intervened by auricular point sticking plus basic nursing, while the control group was by basic nursing alone. The major symptom scores were observed before intervention and after 2-treatment courses, and the clinical efficacies were compared between the two groups.Result Respectively after 1 and 2 treatment courses, there were significant differences in comparing the major symptom scores between the two groups (P<0.05). The total effective rates were respectively 60.0% and 67.5% in the treatment group respectively after 1 and 2 treatment courses, versus 37.5% and 45.0% in the control group, and the differences were statistically significant (P<0.05). Conclusion Auricular point sticking is an effective approach in treating constipation of excessive syndrome in acute stage of stroke.

Objective To investigate the effects of different concentrations of ketamine on the differentiation of human T helper (Th) cells.Methods Twenty ASA Ⅰ patients (aged 20-60 years) undergoing elective operation under general anesthesia were enrolled in this study.Peripheral venous blood samples were taken before anesthesia.Peripheral blood mononuclear cells were isolated and divided randomly into three groups (n =20 each):being incubated in the presence of 0.9％ NaCl (group C),2.5 μg/ml ketamine (group K1) and 25.0 μg/ml ketamine (group K2),respectively,for 24 hours,and were then stimulated with phytohaemagglutinin for 48 hours,respectively.The percentages of Th1 and Th2 cells were detected by four-color fluorescence flow cytometry.The Th1/Th2 ratio was calculated.The levels of interleukin-2 (IL-2),IL-4,IL-6,IL-10,immunoreactive fibronectin-γ (IFN-γ) and tumor necrosis factor-alpha (TNF-α) in the supernatant were determined by cytometric bead array.Results There was no significant difference in the levels of IL-2,IL-4,IL-6,IL-10,IFN-γ and TNF-α in the supernatant,the percentages of Th1 and Th2 cells and the ratio of Th1/Th2 among groups C,K1 and K2(P＞ 0.05).Conclusion The sedative and anesthetic concentrations of ketamine exert no effect on the differentiation of human Th cells in vitro.

Objective To investigate whether intraoperative hypotension was associated with postoperative delirium in older patients undergoing laparoscopic surgery with prospective cohort study.Methods Study subjects were patients,＞ 65 years of age,undergoing laparoscopic surgery,who were enrolled in an ongoing prospective observational study of the pathophysiology of postoperative delirium.Intraoperative blood pressure was measured,and predefined criteria were used to define hypotension.Delirium was measured by the Confusion Assessment Method on the first two postoperative days.Data were analyzed with t-test,two-sample proportion test,and ordered logistic regression muhivariable model,including correction for multiple comparison.Results Data from 213 patients with a mean age of 73.6 years (SD 6.1) were studied.Of these 63 (29.6％) developed delirium on day 1 and 61 (28.6％) on day 2.Relative hypotension (decreases by 20％,30％,or 40％) or absolute hypotension [mean arterial pressure (MAP) ＜ 50mmHg] were not significantly associated with postoperative delirium,nor was the duration of hypotension (MAP ＜ 50 mmHg).Conversely,intraoperative blood pressure variance was significantly associated with postoperative delirium.Conclusions These results showed that increased blood pressure fluctuation,not absolute or relative hypotension,was predictive of postoperative delirium.