Rapid economic development in China gives rise to a growing awareness of patients'rights, resulting in more disputes in medical malpractice between patients and hospitals and deteriorating the once harmonious doctor-patient relationship. The paper discussed the content, influencing factors of a harmonious relationship from both macro and micro perspectives. It focused on the role of governments in building and safeguarding a harmonious relationship, proposing the government should endeavor to create a favorable social environment while the hospital should enhance its management to safeguard a smooth medical process.

To introduce HUANG Hai-long's clinical experience of applying insect drugs on treatment of 8 cases of trifacial neuralgia,facial nerve paralysis,lacouna cerebral embolism,sciatica,acne,xerosis cutis and pruritus symptoms of old people,cirrhosis,refractory amenorrhea.The paper reflects the reliabibity and adaptability of insect drug in treating different diseases with the same syndrome,and the flexible application of insect drugs by HUANG Hai-long.

Though the clinical observation of 413 cases of epidemic hemorrhagic fever(EHF) by clinical epidemiological method,we have concluded that the main TCM etiological factors of EHF are damp,toxic heat,blood stasis and deficiency, and the determinant pathogenesis of EHF is the integration of interior and exterior pathogenic factors;the syndrome characteristic of EHF is that the cold,heat,deficiency and excess were intermingled,and the moist heat blood stasis syndrome should be given priority to;its major pathological location is initial YANG and triple warmer;so the therapeutic principle should be the integration of cold-warm syndrome and exterior and interior and paying attention to smoothing the triple warmer all the time.

China ; Humans ; Organ Transplantation ; legislation & jurisprudence ; Tissue and Organ Procurement ; legislation & jurisprudence

BACKGROUND: AdiponecUn (apM1) has been a new target for gene therapy of ischemic diseases; apM1 gene cloning is the key of successful apM1 gene therapy. There are two main gene cloning methods: directional cloning and T-A cloning method. Directional cloning method is complicated, while the T-A cloning method is relatively simpler, with higher successful rate. OBJECTIVE: T-A cloning of apM1 gene coding region was applied to verify the sequence in comparison with GenBank. DESIGN, TIME AND SETTING: The gene verification experiment was performed at the laboratories of Fujian Hypertension Research Institute which is in The First Affiliated Hospital of Fujian Medical University and Academy of Integrative Medicine which is in Fujian University of Traditional Chinese Medicine, from June 2006 to December 2008. MATERIALS: Adipose tissue sample of omental fat pad was obtained from a surgical patient in the First Affiliated Hospital of Fujian Medical University. Trizol produced by Invitrogen company; M-,-MLV, Gel Extract Kit produced by PROMEGA company; Taq enzyme produced by TIANGEN company; Restriction Endonucleases BamH Ⅰ and Sal Ⅰ, pMD18-T Vector produced by TAKARAcompany were used in this study.METHODS: Total mRNA was extracted from human greater omentum adipose tissue. The coding region of human apM1 (hapM1) gene was amplified by RT-PCR. The coding region of hapM1 gene was cloned into pMD18-T vector .The recombinant plasmid was identified with restriction enzyme digestion analysis and nucleotide sequencing. MAIN OUTCOME MEASURES: The electrophorasis verification of the recombinant plasmids coding target gene using double enzyme digestion, comparison of the similarity between the sequences of the plasmids and hapM1 in GenBank. RESULTS: Sense and antisense coding region of hapM1 gene were cloned. The sequencing results showed that the sequences of the cloned DNA were completely identical to that of hapM1 in GenBank.CONCLUSION: The coding region of hapM1 gene was successfully cloned.

Aim To investigate the expression of the lentiviral vector carrying a human apM1(hapM1) gene in 293 T cells and its bioactivity.Methods The CDS region of hapM1 gene was subcloned into the lentiviral vector from a cloning vector,and the transfer plasmid(PNL-hapM1-IRES2-EGFP)of the lentiviral vector was constructed.The three plasmids of the lentiviral vector: PNL-hapM1-IRES2-EGFP,HELPER,and VSVG were cotransfected to 293 T cells to produce the recombinant lentivirus;293 T cells were infected by the recombinant lentivirus and were identified with fluorescent microscope,RT-PCR and western blot.Inhibitory effect of the recombinant hapM1 on proliferation of vascular smooth muscle cells(VSMCs) was identified.Results The sequencing results showed that the sequence of the subcloned hapM1 gene was completely identical to that reported in GeneBank.The virus titre of the recombinant hapM1 lentivirus was 2.0?105 TU?L-1.After the recombinant lentivirus infected 293 T cells,RT-PCR and Western blot results showed that the expression of hapM1 in the hapM1-293 T group could be measured.The recombinant hapM1 inhibited the proliferation of VSMCs.Conclusions The recombinant lentivirus carrying a human apM1 gene was constructed successfully.The recombinant hapM1 could be expressed in 293 T cells and had its bioactivity.

Objective To evaluate the curative effect of reoperation on postoperative recurrent hepatocellular carcinoma (HCC). Method From Jan.1995 to Dec. 1998, 18 recurrent HCC patients underwent 27 sessions of reoperation in our hospital. Result The mean interval between first and second operation among 18 patients was 39?14 months, and for the 9 cases, in which cancer recurred after second surgery, the mean interval between the second and the third operations was 21?4.5 months (P

China ; Humans ; Liver Transplantation ; ethics ; legislation & jurisprudence ; Tissue Donors ; Tissue and Organ Procurement ; ethics ; legislation & jurisprudence

Hepacivirus ; Hepatitis C ; genetics ; Humans ; MicroRNAs ; genetics

Immune thrombocytopenia (ITP) is the most common hemorrhagic disease in childhood,and immunological abnormality is the most common cause of this disease.Humoral immune abnormalities are the major cause of ITP,involving platelet,platelet membrane surface glycoproteins associated antibodies,impaired Breg cells and upregulation of platelet Toll like receptors.Cellular immune abnormalities include the imbalance of Th1/Th2 cells results in increased inhibitory cytokines [interleukin (IL)-2,interferon-α,tumor necrosis factor-β],IL-17 and Th17 cells in patients with chronic ITP are higher than those in control group,and Treg decline is also common seen in ITP.Increased expression of apoptosis related genes Bak and Bax are also the cause of chronic ITP.Helicobacter pylori is an important cause of chronic ITP,which can be eased in some cases but not effective in new diagnosed cases.Vaccination can lead to ITP,repeated vaccines are applied usually in 3-6 months after the onset.