Objective To characterize the molecular genotypes and quantitative serological biomarker, and to explore their clinical significance in patients with chronic hepatitis B ( CHB) . Methods A total of 210 CHB patients were enrolled and HBV DNA loading, HBV molecular genotypes, quantitative serological biomarker, and ALT were measured. Patients were grouped according to the natural phases of HBV infection:HBeAg (+) immune tolerance, immune clearance, HBeAg (-) low replicative and reactivation phase. The correlation was analyzed between molecular genotypes and serological biomarkers, HBsAg and other biomarkers. Results Subgenotypes B2 with high level of HBV DNA and C2 with middle level of HBV DNA were found to be most prevalent. The positive rates were 59. 0%(B2) and 25. 2%(C2). Patients genotype with B showing more potent viral activity. Subgenotype B2 was sinificantly corelated with HB-sAg, HbeAg, and ALT levels(P < 0. 01), but there was no statistical difference for subgenotype C2. There was statistical difference be-tween positive rates of subgenotypes(P>0. 05), but HBsAg level was found to be significantly correlated with HBV DNA, HBeAg, ALT levels during some phases of CHB. Conclusion Molecular genotyping and quantitative serological biomarkers detection might be helpful for earlier prediction of the long-term disease outcomes in patients with CHB.

Objective:To explore therapeutic effect of noninvasive ventilator on advanced aged patients with acute de- compensated chronic heart failure.Methods:A total of 196 advanced aged patients with acute decompensated chron- ic heart failure hospitalized in our department were randomly and equally divided into routine treatment group and noninvasive ventilator group (received auxiliary treatment with noninvasive ventilator based on routine treatment group).Total effective rate,respiratory rate (RR),heart rate (HR),mean arterial pressure (MAP),partial pres- sure of oxygen in artery (PaO2 )and left ventricular ejection fraction (LVEF)were observed and compared between two groups before and after treatment. Results:Compared with routine treatment group, total effective rate (73.47% vs.90.82%)significantly rose in noninvasive ventilator group,P=0.007. There were no significant difference in above each indexes between two groups before treatment (P>0.05).Compared with before treat- ment,there were significant reductions in RR,HR and MAP,and significant rise in PaO2 and LVEF in both groups after treatment,P<0.05 or<0.01;compared with routine treatment group after treatment,there were significant reductions in RR [(27±6)times/min vs.(19±3)times/min],HR [(109±10)beats/min vs.(73±8)beats/min] and MAP [(110.5±9.0)mmHg vs.(81.6±8.0)mmHg],and significant rise in PaO2 [(77±8)mmHg vs.(98 ±9)mmHg]and LVEF [(41.9±5.4)% vs.(52.7±5.5)%]in noninvasive ventilator group,P<0.05 or<0.01. Conclusion:Noninvasive ventilator treatment based on routine therapy can effectively relieve symptoms of heart fail-ure,improve RR,HR,MAP,PaO2 ,LVEF and cardiac function in advanced aged patients with acute decompensat- ed chronic heart failure,which is worth extending.

BACKGROUND:When peri-implantitis occurs, the levels of interleukin-17 in the peri-implant sulcular fluid and soft tissues are significantly increased, and there is a positive correlation with the depth of the peri-implant probing. However, because of the dual effects of bone absorption and protection, the function of interleukin-17 in peri-implantitis remains controversial, which should be further studied. OBJECTIVE:To retrospectively analyze the correlation between peri-implantitis and interleukin-17. METHODS: The first author searched Wanfang, CNKI and Medline databases from 1991 to 2014 by using key words of “dental implants, peri-implantitis, periodontitis, Th17 cels, interleukin-17, cytokines” in Chinese or English. The functions of bone destruction and protection of interleukin-17 in peri-implantitis were reviewed, and interleukin-17 expression and correlation with the peri-implantitis were detected. RESULTS AND CONCLUSION:According to the inclusion and exclusion criteria, 46 articles from 241 articles are selected. The results show that, noninvasive detection of the variation of interleukin-17 levels in peri-implant sulcular fluid or blood is conducive to the monitoring of early diagnosis and treatment of peri-implantitis. At present, most researches show the relationship between interleukin-17 and peri-implantitis, and there is a positive correlation between interleukin-17 and peri-implant probing depth. But other researches suggest that interleukin-17 acts as protector for bones, and there is no statistic significance in the level of interleukin-17 receptor gene in chronic peri-odontitis and healthy subjects. Therefore, the correlation between peri-implantitis and interleukin-17 remains to be further studied.

Objective To study the relationship between severity of coronary artery lesion and serum level of apolipoprotein A5 (ApoA5). Methods The level of serum total cholesterol (TC), triglyceride (TG), high-den-sity lipoprotein cholesterol (HDL-C ), low density lipoprotein cholesterol (LDL-C ), apolipoprotein A1 ( APOA1), apolipoprotein B (APOB) ,Lipoprotein(a) and uric acid(UA) were examined in 114 patients with coronary heart disease(CHD) and 40 healthy control subjects;Enzyme-linked immunosorbent assay (ELISA) methods was used to determine APOA5. The eoronay heart disease patients were divided into tree groups by the severity of coronary artery lesion: that is one, two and three vessel lesion. Results Compared with control group, APOA5, ApoA1, HDL-C lev-el of CHD groups were lower(P <0.01 or P<0.05) ,TG ,LP(a)and UA were higher(P <0.01 or P <0.05) ,the difference of TC, LDL-C and APOB were not significant (P>0.05 ). In the subgroups of CHD patients, The serum APOA5 concentrations were signficant different between the CHD patients and control group( F=18.605 ,P<0.01 ). Along with the severity of coronary artery,the level of ApoA5 concentrations had a lower trend. The level of ApoA5 was negatively correlated with serum TG level ( r=-0.208, P=0.040) and LP (a) ( r=-0.088, P <0.001). The level of APOA5 had a positive correlation with the serum HDL-C (r= 0.241, P = 0.016). Conclusion There is negative correlation between severity of coronary artery lesion and serum level of ApoA5. The decrease of ApoA5 maybe a risk factor.

Objective: To explore the therapeutic effect of early rehabilitation on aged patients with acute myocardial infarction (AMI) complicated heart failure (HF). Methods: According to number table method, a total of 168 AMI cases were randomly divided into early rehabilitation group (n=84, received early rehabilitation protocol) and routine treatment group (n=84, received traditional treatment). Incidence rate of complication, bicycling test and walking test result were compared between two groups at the end of rehabilitation program. Results: There were no significant difference in angina pectoris after myocardial infarction, re-infarction, dead cases during hospitalization and left ventricular ejection fraction （LVEF）etc. between two groups (P>0.05 all). Compared with routine treatment group, there were significant rise in completion rates of bicycling test (77.6% vs. 95.0%) and walking test (65.4% vs. 95.8%) at the end of program in early rehabilitation group, P<0.01 both. Incidence rates of complications caused by long-term lying in bed in early rehabilitation group were significantly lower than those of routine treatment group (P<0.05 or P<0.01). Conclusion: It’s safe and beneficial to perform early rehabilitation protocol in aged patients with acute myocardial infarction complicated heart failure (35%

More people focus on the cultivation quality of graduate students.The performance of moral character is a very important aspect in all-round performance of college students.It is a definite option to develop moral character in the course of performance education.Scientific quality is also very important.

Objective This paper is mainly to discuss accuracy and clinical application value of MDCT double-period enhanced scanning with low -tension water enteroclysis for colon cancer preoperative TNM stag-ing.Methods Sixty-two colon cancer patients with complete images and pathological data were selected in our hospital from January 2012 to May 2013 .We retrospectively analyzed CT image changes of the tumor location ,the extent of tumor invasion,the surrounding fat space,lymph node metastasis and distant metastasis.We compared them with postoperative pathology to prove the accuracy of MDCT double -period enhanced scanning with low -tension water enteroclysis.Results The results showed that its accuracy rate reached to 90.32%(56/62)in co-lon cancer preoperative Stage T,80.64%(50/62)in Stage N,and 100%(62/62)in Stage M respectively.Con-clusions MDCT double-period enhanced scanning with low -tension water enteroclysis can accurately display the site of colon cancer and determine the scope of tumor invasion ,lymph node metastasis and distant metastasis , and give more precise diagnosis of colon cancer and preoperative staging assessments .In conclusion , it can be used as the preferable method of preoperative examination in the colon cancer .

Objective To investigate the process of apoptosis in lungs and liver induced by crushing hindlimbs of rat, and study the mechanismof crush injury. Methods The rat experimental model of hindlimbs crush injury was established. The cell apoptosis in lungs and liver was detected by TUNEL assay, and the expression of Bax, Bcl-2 and caspase-3 apoptin was examined by immunohistochemistry. Results C ompared with the control group, the partial muscle injury of rat’s hindlimbs was more serious with more apoptosis observed in lungs and liver (P<0.05). The expression of Bax was up-regulated and Bcl-2 was down-regulated, whereas caspase-3 expression was activated (P<0.05). Conclusion The cell apoptosis has increased significantly in lungs and liver after crush injury of hindlimbs in rat. The corre-lation factor released during tissue injury may mediate apoptosis process.

Objective To detect the expressions of BMPR Ⅰa of BMPs/BMPR/Smads signaling transduction pathway in human brain glioma ,to research the role of its in tumorigenesis and progression of brain glioma and its correlation to clinical pathology . Methods The mRNA and protein expressions of BMPR Ⅰa was detected in 20 normal brain tissues and 40 samples with human glioma by RT-PCR and SABC immunohistochemical methods ,repectively ,and analyzed the correlation with the patient′s age ,gender and pathological grade .Results Compared with normal brain tissues ,BMPR Ⅰa mRNA and protein expressions in human glioma were reduced significantly ,especially in Ⅲ and Ⅳ stage tumor tissues .The difference was not related with the patient′s age and gender .Conclusion In progression of glioma ,BMPR Ⅰa may play a role in restraining ,and has nothing to do with age and sex .

PurposeTo evaluate the effect of right ventricular septum (RVS) and right ventricular apex (RVA) pacing on the left atrial systolic function in patients with atrioventricular block by using real-time three-dimensional echocardiography (RT-3DE).Materials and Methods Fifty-one patients with atrioventricular block who were candidates for implanted atrioventricular sequential pacemaker were randomly divided into RVS group (n=31) and RVA group (n=20). The minimum left atrium volume (LAVmin), maximum left atrium volume (LAVmax), and left atrium volume before contraction (LAVprep) were measured by RT-3DE at pre-operation, the 1st month, 3rd month, 6th month, and 12th month after pacemaker implantation. Accordingly, the left atrial total ejection fraction (LATEF) and the left atrial active ejection fraction (LAAEF) were calculated.Results The LAVmin at the 3rd month, 6th month, 12th month after implantation were significantly lower than that at the 1st month and pre-operation in both groups (RVA group:t=2.97 and 2.74,P<0.05; RVS group:t=3.24 and 2.86,P<0.05). LAVprep at the 6th month, 12th month in RVA group, and LAVprep at the 3rd month, 6th month, 12th month in RVS group reduced when compared with that of pre-operation (RVA group:t=3.20,P<0.05; RVS group:t=2.71,P<0.05). LATEF and LAAEF in both groups at the 3rd month, 6th month, 12th month increased when compared with that of pre-operation (RVA group:t=2.87 and 9.68,P<0.05; RVS group:t=3.56 and 8.22,P<0.05). The LATEF and LAAEF in RVS group at the 6th month and 12th month after implantation were significantly larger than that in RVA group at the same time (t=2.90, 5.22, 3.03 and 3.55, P<0.05).Conclusion Atrioventricular sequential pacing is helpful to recovering the left atrial systolic function in patients with atrioventricular block. Compared with RVA pacing, RVS pacing is able to increase LATEF and LAAEF more greatly.