Humans ; Hypoxia-Ischemia, Brain ; diagnosis ; therapy ; Infant, Newborn ; Infant, Premature

Cardiovascular Diseases ; psychology ; Humans ; Life Style

Objective To establish a thin layer chromatography scanning method for the determination of cinnamaldehyde content in Qianzheng Plaster.Method Cinnamaldehyde was extracted from Qianzheng Plaster by distillation method.Silica G was used as the thin-layer plate;petroleum ether (60～90℃) and acetic ethyl acetate (17 ∶3)served as the mobile phase and the scanning wavelength was 269 nm.Results A good linearity was obtained in the range of 0.81?g～4.05 ?g (r=0.9993).The mean recovery rate was 95.23 %and RSD=1.39 %.Conclusion This method is accurate and sensitive with good reproducibility.

Constipation ; diagnosis ; therapy ; Humans ; Medicine, Chinese Traditional ; Rectum ; physiopathology ; Syndrome ; Touch

Brachytherapy has become an important treatment for soft tissue sarcoma by its unique radio-biology and radiation physics characteristics, the treatment time is divided in accordance with preoperative brachytherapy, intraoperative brachytherapy, postoperative brachytherapy, brachytherapy alone, which in im-proving the rate of limb preservation and local control rate sarcoma has played an important role. However, the timing of treatment, treatment modalities, the choice of implantation dose is still in dispute.

ObjectiveTo observe the influence of triamcinolone acetonide (TA) on the expression of pigment epithelium-derived factor (PEDF) of human retinal pigment epithelial (RPE) cells. Methods Cultured human RPE cells (4th - 6th generations) were treated with four different concentrations of TA (40, 400, 4× 103 and 4× 104 μg/L) for three different periods (12 or 24 or 48 hours), the levels of PEDF protein in the cell culture supernatant and cell lysates were determined by Western blot. After the initial experiment, RPE cells were treated with or without tumor necrosis factor-α (TNF-α, 20 ng/ml) for 24 hours, followed by TA (400 μg/L) treatment. The levels of PEDF and phospho-p38 mitogen activated protein kinase (p-p38MAPK) protein expression in cell culture supernatant and cell lysates were measured by Western blot. Results TA-treated RPE cells had higher PEDF expression, and 400 μg/L TA group had the highest effect (F= 16.98, P＜0. 05). 400μg/L TA treatment for one, six or 24 hours, with or without TNF-α pretreatment, could all promote the PEDF expression and inhibit the p-p38MAPK protein expression (F= 16.87, 10.28; P＜0. 01). TNF-α pretreatment alone could inhibit PEDF protein expression and promote p-p38MAPK protein expression (F= 16.87, 10. 28; P＜0. 01). Conclusions TA can up-regulate the expression of PEDF, and down-regulate the expression of p-p38MAPK in the cultured human RPE cells.

Asphyxia Neonatorum ; therapy ; Humans ; Infant, Newborn ; Infant, Newborn, Diseases ; Neonatology ; methods ; Resuscitation ; methods

Objective:To study the expression of integrin ?_5,?_3 in eutopic endometrium and ectopic endometrium of endometriosis. Methods:Reverse transcription polymerase chain reaction(RT-PCR) method was utilized to detect and quantitate integrin ?_5,?_3 mRNA expression levels in eutopic and ectopic endometrial tissues from 18 patients with endometriosis and the endometrial tissues from 18 non-endometriosis women.Serum concentration of estradiol(E_2),progesterone(P) and CA125 were measured by radioimmunoassay.AFS-r marking was used to describe the state of pelvic cavity during operation. Results:Various levels of positive expression of integrin subunits ?_5,?_3 were shown in both eutopic and ectopic endometrial tissues of endometriosis and the endometrium of non-endometriosis.The expressions of integrin ?_5 mRNA in eutopic endometrium,ectopic endometrium of endometriosis group and endometrium of control group were as 0.773?0.113,1.077?0.032,0.924?0.120,respectively.(Among) the groups,the differences were statistically significant(P0.05).Both CA125 level and AFS-r were positively correlated with integrin ?_5,?_3(P

Objective To establish a rapid ,sensitive direct method for detecting Escherichia coli (E .coli) in the positive blood culture bottles by using the loop‐mediated isothermal amplification (LAMP) .Methods Based on the lacZ (accession number :M74750) gene sequence of E .coli in the gene sequence GenBank by the National Institutes of Health (NIH) ,4 specific primers (2 inner primers and outer primers) were designed ,the lacZ gene was amplified and the amplification reaction was optimized .The sen‐sitivity and specificity of this method were verified and its comparison with the traditional culture method was conducted .Results Sixty‐eight strains of E .coli were detected in 300 positive blood culture bottles by adopting the LAMP method .The designed prim‐ers had good sensitivity and good specificity for the amplification of E .coli .The sensitivity and specificity of LAMP all reached 100% compared with the traditional method ,but the traditional method needed 3 d to get the results ,while LAMP only needed 1 h . Conclusion LAMP is extremely rapid for detecting E .coli with low cost ,strong specificity and high sensitivity and is suitable for clinical application .

Objective To investigate the application value of prescription examination in the management of antimicrobial agents in hospital.Methods The prescriptions of antibacterials were examined,the irrational use of drugs was counted,and the application value of drug prescription examination in the management of antibacterial drugs in hospital was discussed.Results A total of 488 960 prescriptions were examined,including 133 534 prescriptions for antimicrobial agents,and 27.31％ for antimicrobial agents.Unqualified antibiotics use prescription in 60 086,unqualified rate was 45.00％,among them not standard prescription in 108,occupied 0.18％,medication unsuitable prescription in 59 907,occupied 99.70％,exceed prescription in 71,occupied 0.12％.Conclusion There is an irrational use of antibiotics in outpatient service,and the examination of antibacterial prescription should be strengthened so as to improve the level of rational use of antibiotics in hospital.