Objective To observe the efficacy of comprehensive strategies in treating diabetic foot.Methods Thirty five cases with diabetic foot were treated by comprehensive strategies,including antiinfection,strict blood sugar control,vasodilatation,improvement of microcirculation,never nutrition,debridement and supportive care,etc.Results Among the 35 patients with diabetic foot,24 were recovered satisfactorily,7 cases were recovered with improvement,2 cases underwent amputation and 2 cases gave up further treatment.Conclusion Diabetic foot can be treated by comprehensive measures satisfactorily,with improved cure rate and low amputation rate.

The serum suppressive activity on PHA-induced lymphocyte transformation of healthy individuals was evaluated in 76 serum samples from 55 patients with systemic lupus erythematousus(SLE).The serum suppressive activity seemed well correlated with the activity of SLE. The highest levels of suppressive rate (55.02+29.62％) and positive rate(88.33％)were found in patients with active disease. Once disease improved, both suppressive rate and positive rate were reduced (22.17?51.28％ and 51.85％ respectively), when the patients entered into stationary stage, the serum suppressive activity were not observed. The correlation between suppressive activity and lymphocyte transformation test, E-rosett inhibition factor, the levels of serum CIC, IgG, IgA, IgM. C3 and ANA titers were analysed. Stepwise discriminant analysis indicated that the serum suppressive activity was more closely related to disease activity than other immunological indexes. The nature of suppressive factor was discussed.

Henoch-Schonlein purpura(HSP) is one of the most common blood capillary allergic disorder in children, and its pathogenesis has not been well explained. The studies show that the main mechanism of HSP is the abnormality of humoral immunity, and the role of IgA is emphaszed. Meanwhile, many agents such as changes in T cell function,participation of cytokine and mediators of intlammation, suscepti-bility gene and other factors also play an important role in the incidence of HSP. The pathogenesis of HSP can be better understood through extensive analysis of the immunology factors,genetic factors,and so on.

Brachytherapy has become an important treatment for soft tissue sarcoma by its unique radio-biology and radiation physics characteristics, the treatment time is divided in accordance with preoperative brachytherapy, intraoperative brachytherapy, postoperative brachytherapy, brachytherapy alone, which in im-proving the rate of limb preservation and local control rate sarcoma has played an important role. However, the timing of treatment, treatment modalities, the choice of implantation dose is still in dispute.

Recent studies about stem cells show that the formation of portal vein tumor thrombus(PVTT)is closely relative to the metastasis of liver cancer stem cells induced by portal vein microenvironment.Liver cancer stem cells first detach from the primary tumor,extravasate to extracellular matrix,stimulate angiogenesis or intravasate and migrate through the systemic circulation,evade host defense mechanisms,migrate to specific blood vessel sites through chemo-taxis and attach to blood vessel wall,then extravasate through blood vessel and home to specific microenvironment and to form PVTT.

Objective To observe the effect of wheel running exercise on subventricular zone neurogenesis and the neural function in rats post cerebral ischemia. Methods 48 adult male Sprague-Dawley rats were induced cortical infarcts with left middle artery occlusion and were housed in either standard (control group, n=24) or wheel running (exercise group, n=24). They were assessed with neurological severi-ty scores (NSS), and the expression of Ki67 was determined with immunofluorescence, 3, 7, 14 and 21 days after training. Results Com-pared with the control group, the number of Ki67-labeled cells in subventricular significantly increased in the exercise group 14 and 21 days after ischemia (P<0.05), and the NSS decreased since 7 days after ischemia (P<0.05). Conclusion Wheel running may promote the neuro-genesis in subventricular of adult rats after cerebral infarction, which may associate with the recovery of neural function.

Objective To prepare the specific monoclonal antibodies(mAb) of Mycoplasma pneumonia(MP) and to establish a colloidal gold rapid detection method of MP by using mAb .Methods The nasopharyngeal swab samples were collected from chil‐dren patients with acute respiratory tract infection ,separated and cultured and performed the MP identification ,MP antigen was pu‐rified ,mAb of MP was prepared ,then the MP colloidal gold test strip was established by using MP mAb .The throat swab sample was collected from the patients with suspected MP infection and detected by using the fluorescent quantitative PCR and colloidal gold test strip .The detection results were observed and statistically analyzed .Results MP strain was successfully isolated and inac‐tivated ,the MP antigen was purified .The mouse was immunized by using this antigen ,19 strians of mAb against MP were prepared by using the hybridoma technique .Among them ,2 strains of mAb with high titer and good specificity(MP‐5 and MP‐19) were se‐lected as the raw materials for preparing the MP colloidal gold rapid test strip .The lowest detection limit was 20 ng .The clinical samples were detected by using the MP colloidal gold rapid test strip and fluorescent quantitative PCR .The results showed that the sensitivity of MP colloidal gold rapid test strip by using this established method was 88 .2% and its specificity was 82 .6% .Conclu‐sion specific mAbs against MP is prepared and the colloidal gold rapid detection method is preliminarily established which provides the help for rapid diagnosis in the patients with MP infection .

Objective: To compare the ocular parameters of acute angle closure glaucoma (AACG) and chronic angle closure glaucoma (CACG). Methods: Totally 106 patients with primary angle closure glaucoma were recruited: 58 patients with AACG and 48 with CACG. All patients were divided into 3 groups: AACG attack eyes group, AACG uninvolved fellow eyes group and CACG group and underwent the same ophthalmic examinations, comprising optometry, keratometry, and A-scan ultrasonography. The lens/axial length factor (LAF) and relative lens position (RLP) were calculated. Results: The AACG attack eyes had a significant shallow anterior chamber depth, thick lens, short axial length and larger LAF. hTere tended to be a reduction in the percentage of LAF>0.20 in AACG attack eyes, CACG eyes and AACG uninvolved fellow eyes, though there were no statistically signiifcant difference in all groups (P>0.05). Conclusion: The eyes with AACG attack have a more crowded anterior chamber structure compared with uninvolved fellow eyes and eyes with CACG.

Lung cancer is the leading cause of morbidity and mortality of malignant tumor.Surgery,radiotherapy and chemotherapy remain the primary means of treatment for lung cancer,while the discoveries of molecular targets and targeted agents for lung cancer are making revolutionary changes and will almost certainly propel progress in this field into the foreseeable future.Biomarker-based individualized comprehensive treatment strategy is expected to benefit the lung cancer patients' survival.Meanwhile,chemotherapy remains the major treatment of advanced non-small cell lung cancer,which requires implementing the "three combinations" dialectical therapy principles,that is,systemic with local,conventional with personalized,eliminating with supporting.