1.Application of the Serum Protein Changes and it′s Protein Electrophoretic Analysis in Patients with Fever of Unknown Origin.
Jicheng XING ; Yuerong ZHU ; Hong QIU
Journal of Medical Research 2006;0(02):-
Objective To study the diagnostic value of detecting pre-albumin,albumin and protein electrophoresis in the serum of patients with fever of unknown origin.Methods Serum pre-albumin,albumin and protein electrophoresis were detected in 86 patients with fever of unknown origin and 26 controls.Results The level of albumin and pre-albumin in fever of unknown origin group was lower than that of control group(P
2. Application of chromosome microarray analysis in neonatal birth defects
Jian LU ; Xing LI ; Weiwei HUANG ; Yi LI ; Jicheng WANG
Chinese Journal of Applied Clinical Pediatrics 2019;34(16):1231-1233
Objective:
To explore the application of chromosomal microarray analysis (CMA) in neonatal birth defects and further determine the frequency of chromosome imbalances in neonates with birth defects.
Methods:
Retrospective analysis was performed in 121 neonates with specific features, such as distinctive facial features, congenital heart disease, congenital malformation, neonatal decreased responsive, hypotonia, seizures and others at the Department of Neonatology, Guangdong Women and Children Hospital from May 2016 to November 2017.All the cases were analyzed by using chromosomal microarray analysis (CMA).
Results:
A total of 23 (19.0%) patients were identified with pathogenic CNVs, 3 patients (2.5%) with uncertain clinical significant CNVs.There were 5 patients (4.1%)with chromosome numerical abnormalities, 12 patients (9.9%)with microdeletion/microduplication syndrome, 6 patients (5.0%) with chromosome deletion or duplication.All groups whose incidence was sorted from high to low were facial characters(30.3%), congenital malformation(21.6%), neonatal decreased responsive(9.1%) and other indications(6.7%).
Conclusions
CMA is a valuable clinical diagnostic tool that allows precise identification of chromosome imbalances as the cause of birth defects in neonates.CMA can detect many more clinically relevant genomic abnormalities than conventional cytogenetic study and assist the clinician in diagnosis, early neonatal intervention, and genetic counseling.
3.Regulation of miR140-5p for paraoxonase 1 expression in HepG2 cells and its clinical application
Jiaxing LIU ; Bing WANG ; Jicheng XING ; Yujie HE ; Aizhen YANG ; Hong QIU
Chinese Journal of Clinical Laboratory Science 2019;37(2):137-141
Objective:
To investigate the effects of miRNA on the expression of paraoxonase 1 (PON1) and its clinical application in the patients with nonalcoholic steatohepatitis (NASH).
Methods:
Bioinformatics methods were used to analyze and predict PON1 related regulation on miRNA. PON1 luciferase reporter gene vectors were constructed and the activity of dual luciferase was analyzed. The up/down-regulated levels of miRNA in HepG2 cells of different groups were detected by real-time fluorescence quantitative PCR (qRT-PCR), and the levels of PON1 protein in HepG2 cells were detected by western blot. The levels of miR140-5p in the serum of healthy people and NASH patients were also analyzed by qRT-PCR.
Results:
According to the prediction of TargetScan database, miR140-5p may bind complementarily to the end of PON13′-UTR. The analysis for the activity of dual luciferase reporter gene showed that miR-140-5p mimic significantly downregulated the fluorescence of wild type PON1 vector (P<0.01). The results of qRT-PCR demonstrated that miR-140-5p mimic group showed high overexpression (P<0.01) compared with the normal cell control group and the negative mimic control group, while miR-140-5p inhibitor group appeared corresponding low expression (P<0.05). western blot results suggested that the transfection of miR140-5p mimic significantly down-regulated the expression of PON1 (P<0.01) while miR140-5p inhibitor up-regulated this expression (P<0.01). Compared with the healthy control group, the level of miR140-5p was decreased in the serum of NASH patients, and the difference was statistically significant (P<0.01).
Conclusion
miR140-5p may be involved in the progression of nonalcoholic steatohepatitis through regulation for the posttranscriptional gene expression of PON1.
4. Current status of blood lipids in people with hypertension and diabetes in Henan province
Xinyun LIU ; Wenlu XING ; Jicheng JIANG ; Chuanyu GAO ; You ZHANG ; Shan WANG ; Gang ZHOU ; Lianxin DU ; Chengqi WU ; Gang HOU ; Yuming WANG
Chinese Journal of Cardiology 2019;47(5):360-366
Objective:
To investigate the blood lipid levels and prevalence of dyslipidemia in people with hypertension and diabetes in Henan province.
Methods:
From April 2016 to April 2017, multi-stage cluster sampling was adopted to investigate 71 285 local residents aged between 35 and 75 from 6 districts and counties in Henan province including Zhongmu county of Zhengzhou city, Huojia county of Xinxiang city, Hualong district of Puyang city, Qi county of Hebi city, Xigong district of Luoyang city, and Wugang city of Pingdingshan city. Blood samples were collected. According to the diagnostic criteria of hypertension and diabetes, the study population was divided into control group (