Objective:To construct a replicating adenovirus vector CNHK600-p53 carrying p53 gene and investigate its effect on the chemosensitivity of hepatocarcinoma cell line.Methods: Chemotherapy of hepatocarcinoma cells BEL-7404 were carried out using Fluorouracil,Mitomycin,Epirubicin,CNHK600-p53,CNHK600-p53 + Fluorouracil,CNHK600-p53 + Mitomycin,and CNHK600-p53 + Epirubicin,separately.MTT assay was used to evaluate the killing effects after therapy.Results: The inhibition rate on BEL-7404 cells was(47?3)% when using 100 ?g/ml 5-Fu,was(20?4)% when using 1 ?g/ml MMC,and was(73?2)% when using 2.5 ?g/ml EPI.The inhibition rates of BEL-740 cells in CNHK600-p53(MOI=10) + Fluorouracil(100 ?g/ml),CNHK600-p53(MOI=10) + Mitomycin(1 ?g/ml),and CNHK600-p53(MOI=10)+ Epirubicin(2.5 ?g/ml) groups were(59?4)%,(44?4)% and(86?2)%,respectively(P

Objective To summarize and analyze the surgical therapies for liver and gastric multiple primary cancers. Methods The clinical data of 14 patients with liver and gastric carcinomas surgically treated in our hospital from January 2004 to January 2008 were retrospectively analyzed.Results Of the 14 patients, 12 underwent simultaneous resection of liver and gastric carcinomas,1 resection of the gastric carcinoma 2 months after the liver surgery and 1 removal of liver cancer found 2 years after the surgery for antral adenocarcinoma. The median survival time of these patients was 23 months. The 1-and 3-year survival rate was 78.6% and 35.7%, respectively. Conclusion Due to different pathological characteristics, the therapies of liver and gastric multiple primary cancers are completely different from that of the recurring and metastatic carcinoma. Both tumors can be treated by radical resection and the effect is similar to single cancer. Positive treatment is crucial for long-term survival of the patients.

Objective To construct a new replicating adenovirus vector CNHK600-p53 carrying anti-tumor gene p53 and investigate its effect on hepatocarcinoma cell line PLC/PRF5. Methods The methylthiazolyl tetrazolium assay (MTT) was used to observe the killing effect of Fluorouracil, Mitomycin and Epirubicin alone or in combination with adenovirus CNHK600-p53 on PLC/PRF5. Results When 5-Fu at concentration of 400?g/ml, the inhibition rate was (65?4. 2) % ; with MMC at 1?g/ml, the rate was (41?1.9)%; and it was (65?1.8)% when EPI at concentration of 10?g/ml. With PLC/PRF5 infected by adenovirus CNHK600-p53 ( MOI = 0. 625) , the inhibition rate was significantly increased to (89?5. 3)%,(60?2.3)% and (75?1.5)% respectively; When MOI was 0.3125, 0.625, 1.25, the inhibition rate in CNHK600-p53 group and Ad-p53 group was (27?2. 5)% , (30?3. 7)% , (61?4. 3)% and(4?2.7)%, (5?3.5)%, (16?4.5)% respectively. Conclusion For hepatocarcinoma cell line PLC/PRF5 the effect of CNHK600-p53 is stronger than Ad-p53.