[Objective]To design a new kind of instrumentation specially to correct scoliosis and kyphoscoliosis.[Method]Through posterior transpedicular and vertebrae,wedged osteotomy was continually performed until anterior substantia compacta of vertebrae.[Result]More than(1 200) patients with scoliosis or kyphoscoliosis were treated with WVOI.The highest ostentomied vertebrae was thoracic 9 and 4 levels were performed for 1 case(mean 2 levels).The average time of osteotomy on one vertebrae was 30 min with 100 ml blood loss.No spine and spinal root injury was detected during the operating procedure.[Conclusion]Osteotomy with WVOI reduced operating time,blood loss,keep spine and spinal root safer during the operating procedure.It also made osteotomical plate fit well and prompted quick ossification.WVOI is an efficient instrumentation to correct kyphosis and kyphoscoliosis.

[Objective]To study the effect of improved universal pedicle screw systems which combined locking pedicle screws with sliding pedicle screws to correct the spinal deformities in growth period through posterior incision.[Method]From June 2000 to June 2006,209 spinal deformities in growth period were treated(106 males and 103 females),including 92 idiopathic scoliosis,72 congenital scoliosis,26 Scheuermanns diseases and 19 other kinds of spinal deformities.The age of patients ranged from 8 to 16 years(average 13.22 years).Locking pedicle screws were placed into pedicles of apical vertebra and the 3 to 5 adjacent vertebrae.Sliding pedicle screws were putted into pedicles of cephalic and caudal vertebrae.Double cross-links and rods were fixed and locked tightly.Sliding cross-links themselves could cannula prolonged to fit for the transverse development of vertebrae.[Result]Totally 209 cases with preoperative Cobbs angle 45 degree to 110 degree(average 68 degree)were instrumented with improved USS systems and 96 cases were followed up for 26～60 month.The length of spines were prolonged 4 cm to 15 cm with average 7 cm.With 26 months to 60 months follow-up,the whole spine growth was 230.4 cm with average 2.4 cm respectively.Rods broken were found in 6 patients and no other cmplication was detected.[Conclusion]Improved USS system which combined locking pedicle screws with sliding pedicle screws had the three dimensional power and intensity of traditional 3D instrumentations.Sliding pedicle screws and cross-links permitted longitudinal growth of the vertebrae and transverse development pedicles.

[Objective]To investigate the prevalence rate of adolescent scoliosis in Heilongjiang province. [Methods]A total of 17 525 students(aged 7 ~15 years old) from 28 primary and secondary schools were screened for scoliosis from April 2007 to May 2009.Physical examination,corrugated photography and X-ray were used for the screening,prevalence rate was calculated and correspondent treatment was given to patients.[Results]The result of first check-positive was 911(5.20%),with male 448(4.92%),female 463(5.44%).The second check-positive was 413(2.36),with male 181(2.00%),female 232(2.73%),387 in them received the third check.In the third check,335 patients were found the Cobb's angle ≥10?,with the prevalence rate of 1.91%(male 147,1.63%,female 188,2.21%).[Conclusion]Adolescent scoliosis can be early found,early diagnosed,prevented and treated by general survey.

[Objective]To evaluate the role of three radiographic ways in patients undergoing spinal three-dimensional correction and fusion for adolescent idiopathic scoliosis.[Method]Sixty-three cases of adolescent idiopathic scoliosis(AIS)with 79 structural curves were studied.Preoperative X-ray of standing,gravity-suspendig traction,supine bending and fulcrum bending were compared with postoperative standing X-ray one week after surgery,Cobbs angle of all X-ray were measured and statistical significance was evaluated.[Result]In structural thoracic group and lumbar group the mean Cobbs angle were 40?and 21?in gravity-suspendig traction X-ray,41?and 23?in supine bending X-ray,35?and 19?in fulcrum bending X-ray,36?and 18?in postoperative standing X-ray respectively.In severe group and moderate group the mean Cobbs angle were 52?and 23?in gravity-suspendig traction X-ray,53?and 24?in supine bending X-ray,47?and 20?in fulcrum bending X-ray,44?and 19?in postoperative standing X-ray respectively.In rigid group and flexible group the mean Cobbs angle were 51?and 22?in gravity-suspendig traction X-ray,52?and 22?in supine bending X-ray,48?and 18?in fulcrum bending X-ray,45?and 17?in postoperative standing X-ray respectively.In anterior approach group and posterior approach group the mean Cobbs angle were 47?and 15?in gravity-suspendig traction X-ray,49?and 16?in supine bending X-ray,43?and 11?in fulcrum bending X-ray,42?and 10?in postoperative standing X-ray respectively.All above Cobbs angle were mean numerical value,the mean follow-up period was 1.5 years(range:1~3 years).[Conclusion]The fulcrum bending X-ray are more predicting of correctability of AIS patients than the gravity-suspendig traction and supine bending X-ray.It can provide reference for chosing anterior or posterior approach and chosing fusion levels.

Objective To explore the recent and distant effects of early active training after the operation for lumber intervertebral disc herniation.Method 79 patients after the operation for mono segmental lumber intervertebral disc herniation had been divided into early active training group and routine control group randomly, and accepted training, regular re examination, and follow up of 1～ 6 years respedtively.Results The early active training group had better recent and distant objective effect, and more patients (97.6％ ) were satisfied with the operational effects.Conclusions The early active training after the operation for lumber intervertebral disc herniation is positive significant for operational effects.

Objective To investigate the effects of cefoprazone/sulbactam(CPZ/Sul)and dexamethasone(DXM) on serum IL-8,IL-10 in SD rats with severe pneumonia due to Klebsiella pneumoniae.Methods A rat model of severe pneumonia was induced by intratra- cheal instillation of Klebsiella pneumoniae with a dose of 3.6×108CFU(0.3ml).60 male SD rats were random divided into control group,model group,cefoprazone/sulbactam(CF-L/Sul)group and cefoprazone/sulbactam plus dexamethasone(DXM+CPZ/Sul)group.15 rats in each group were then divided into three subgroups and 5rats in each subgroup were examined at the 6th,8th and 10th day,respectively.Arterial gas analysis.total WBC and PMN cell counts in BALF were examined.ELISA method was used to detect the level of IL-8 and IL-10 in serum.RESULTSIn model group,PaO2 were progressively decreased while total WBC and PMN cell counts in BALF were markedly increased.In CPZ/Sul group,all parameters were alleviated,but there were no significant difference of serum IL-8 and IL-10 between model group and CPZ/Sul group.In DXM+CPZ/SUl group,all parameters were significantly alleviated including serum IL-8 and IL-10.Conclu sions Severe pneumonia of rat due to Klebsiella pneumoniae was characterized by significant elevation of serum IL-8 and IL-10,which suggested the imbalance of inflammatory/anti-inflammatory mediators during the course of severe pneumonia.DXM codd markedly decrease the production of inflammatory mediators and slightly decrease the production of anti-inflammatory mediators,which could restore the balance and contribute to the relief of severe pneumonia.

Objective To analyze the data of Human Immunodeficiency Virus (HIV ) screening in Qinnan District of Qinzhou from 2013 to 2015 ,and to provide scientific evidences for the prevention and control of Acquired Immune Deficiency Syndrome (AIDS) .Methods HIV antibody data which was collected from the AIDS laboratory between 2013 and 2015 in Qinnan District of Qinzhou were analyzed .Results Of 3 955 cases of the HIV antibody detecting in the AIDS laboratory from 2013 to 2015 ,200 cases were HIV antibody positive ,and the positive rate was 5 .06% which was decreased compared with previously .The HIV antibody positive rate of man was 9 .26% ,which was higher than women .Among the HIV positive people ,the rate of the ages older than 50 was 25 .36% which was the highest compared to the other age groups .The positive rate of farmers was 30 .83% and the rate of di‐vorced or widowed was 30 .86% and the rate of the illiterate was 16 .08% .Conclusion Man ,farmers ,the age older than 50 years old ,the divorced or widowed and the low degree of education groups are the focus groups of health education and prevention of AIDS .If we can find HIV infectors earlier ,we can control the epidemic of AIDS and formulate the prevention and control strategies better .

Objective To investigate the expression and significance of syntaxin 8 (STX8) in non-small cell lung cancer (NSCLC),and analyze the relationship between STX8 expression and clinicopathological features of NSCLC.Methods Seventy samples of NSCLC and 70 samples of pericancerous tissues were collected for immunohistochemistry staining,and another 10 samples of NSCLC and pericancerous tissues were used for RNA and protein extraction,qRT-PCR was performed to detect the expression of STX8 mRNA,and Western blot assay was adopted to detect the expression of STX8 protein.The relationship between STX8 expression and clinicopathological features of NSCLC was analyzed.Results Results of qRT-PCR showed that the expression of STX8 mRNA was significantly higher in NSCLC tissues than that in the pericancerous tissues (3.962 5±0.487 3 vs.0.538 2±0.097 5,t=21.797,P＜0.01).Immunohistochemistry results showed that the high expression rate of STX8 was significantly higher in NSCLC tissues than that in the pericancerous tissues [71.43％ (50/70) vs.38.57％ (27/70),x2=15.267,P＜0.01].Western blot results showed that the expression of STX8 protein was significantly higher in NSCLC tissues than that in the pericancerous tissues (2.496 1±0.362 5 vs.0.340 2±0.119 1,t=17.876,P＜0.01).The high expression rate of STX8 was significantly different in different histological types of NSCLC (P ＜0.05).The high expression rate of STX8 was higher in squamous cell carcinoma and adenocarcinoma than that in adenosquamous carcinoma,and the high expression rate of STX8 was not observed in large cell carcinoma.There were no significant differences in expressions of STX8 in NSCLC patients with different gender,ages,tumor diameters,TNM stages and with or without lymph node metastasis (P＞0.05).Conclusion A high expression of STX8 in NSCLC tissues may be correlated with the occurrence and development of NSCLC.

BACKGROUND:Standing bending, supine bending, lateral pressing, pronation compression films and traction radiography are indeed effective to predict the correction outcome of surgeries by Harrington's method and Lugue's method,however, they are discontented for the correction of three-dimensional pedicle screw of vertebral arch.OBJECTIVE: To evaluate the role of preoperative X-ray films of gravity-suspending traction, supine bending and fulcrum bending in patients undergoing spinal three-dimensional correction and fusion for adolescent idiopathic scoliosis (AIS).DESIGN: Contrast observation.SETTING: Department of Orthopaedics, the 211 Hospital of Chinese PLA (Military Orthopaedics Center).PARTICIPANTS: From January 2003 to December 2005, 63 cases aged 10-21 years were recruited from Department of Orthopaedics in the 211 Hospital of Chinese PLA, including 18 males and 45 females. They were diagnosed as AIS by clinical examinations and X-ray film, with Cobb angle≥40°. Informed consents were obtained from all the subjects.METHODS: All of the 63 AIS cases with 79 structural curves were studied by preoperative X-ray films of standing,gravity-suspending traction, supine bending and fulcrum bending, which were compared with standing X-ray films one week postoperatively.MAIN OUTCOME MEASURES: Cobb angles of preoperative fulcrum bending film and postoperative standing film.RESULTS: All of the 63 cases were involved in the result analysis. There was no significant difference in the Cobb angle between preoperative fulcrum bending X-ray film and postoperative standing X-ray film (P ＞ 0.05). Gravity-suspending traction and supine bending films were significantly different from postoperative standing film (P ＜ 0.05).CONCLUSION: The fulcrum bending X-ray films are superior to gravity-suspending traction and supine bending films in predicting the postoperative correction of AIS patients.

BACKGROUND: Locking pedicle screw system is commonly used in clinic, but it often suppresses spinal longitudinal growth of adolescent at growth phase. Thus, a pedicle screw system that can reduce even avoid the inhibition to spinal growth is needed. OBJECTIVE: To compare the biomechanical performance of sliding instrumentation of pedicle screw system and traditional locking pedicle screw system. DESIGN: Comparative observation. SETTING: Department of Orthopedics, Xinqiao Hospital of Third Military Medical University of Chinese PLA, and Department of Orthopedics, the 211 Hospital of Chinese PLA. MATERIALS: The experiment was performed at Department of Material Science, Harbin Institute of Technology on June 29th, 2007. Self-designed sliding pedicle screw system was made of Ti alloy by Wujin No. 3 Medical Instrument Factory Co., Ltd., Jiangsu Province. It consisted of sliding pedicle screw, orthopaedic rod and transversal coupling device. Twelve samples of fresh porcine spine were selected, and muscles attached on vertebral bodies of TrL5 were removed carefully but integrity of main ligament and precessus articularis posterior was retained. METHODS: The samples were randomly divided into sliding system group and locking system group with 6 samples in each group. Partial vertebral plate and surrounding ligaments of T12as well as bilateral facet joints between T11-12 and T12-L1 were removed to induce spinal destabilization, then sliding pedicle screw system and locking pedicle screw system were respectively fixed onto T10, T12, and L2 vertebral bodies of two groups. The samples then were fixed into fixture, and put onto INSTAON-4505 axial compressor. The strain gauge was connected with YJ-31 static electricity resistance strain gauge instrument human to simulate human spinal load, and the center of gravity was loaded to induce forward flexion, backward extension, lateral flexion and axial construction. Load of 100, 200, 300, 400 and 500 N was given gradually, and displacement of T12 was measured under different loads. MAIN OUTCOME MEASURES: ①Changns in principal stress and displacement under forward flexion, backward extension, lateral flexion and axial construction; ②Spinal fixation intensity and rigidity. RESULTS: No statistical difference was detected in main straining, displacement of apical vertebrae and intensity of fixation between sliding system group and locking system group under forward flexion, backward extension, lateral flexion and axial construction (P > 0.05). CONCLUSION: Sliding pedicle screw system has identical biomechanical stability as locking system. Furthermore, in sliding pedicle screw system, the screw and rod are coupled by sliding pattern, which extend along with spinal growth. It can be used to treat scoliosis at growth phase.