Objective To explore the role of Th17/IL-17 in the pathogenesis of asthma in children.Methods Thirty asthma children (asthma group) and 20 healthy volunteers (control group) were selected as our subjects.Percentage of Th17 cell in peripheral blood mononuclear cell (PBMC) of asthma children was detected by flow cytometry,the level of IL-17 and IgE in plasma were examined by enzyme linked immunosorbent assay.Results Proportion of Th17 cells in PBMC of children in asthma group was (1.83 ± 1.01) ％,significantly higher than that in control group ((1.02 ± 0.49) ％ ; t =3.896 ; P ＜ 0.01).The IL-17 level in control group was (19.11 ± 3.23) ng/L,significant lower than that in asthma group ((34.23 ± 4.88) ng/L;t =6.261 ;P ＜ 0.01)).The Ig E level in asthma group was (399.4 ± 45.1) ng/L,significantly higher than in control group((58.2 ± 19.7) ng/L;t =7.244,P ＜0.01).The level of IL-17 in plasma had a postive relationship with the proportion of Th17 cells(r =0.882,P ＜0.01),but not relationship was seen with the level of IgE (r =0.375,P ＜ 0.05).Conclusion Th1 7 cell is associated with asthma development of children,and the reason may due to probably aggravating asthma condition through increasing inflammation secreting IL-17.

Language is an unique complex psychological activity of human beings.Language barrier is a decline of effective activities and interactions in supporting the cognitive process of language behavior.Cognitive function is affected to a certain degree in patients with post-stroke aphasia.Therefore,accurately identifying these cognitive impairments contribute to the treatment and rehabilitation of the patients with aphasia.The assessment of the severity of cognitive impairment needs to use the cognitive assessment scales.This article reviews the cognitive assessment scales that are applicable for patients with post-stroke aphasia.

Objective To develop the Non-Language-Based Cognitive Assessment (NLCA) applicable to patients with aphasia and to validate the reliability and validity of NLCA.Methods Seventy-three normal subjects and 32 patients with mild cognitive impairment were evaluated by the NLCA and the Mini-Mental State Examination.Forty subjects were randomly selected from the normal subject samples were assessed with the NLCA,the Auditory Verbal Learning Test,the Rey-Osterrieth Complex Figure Test (Form A),the Stroop Color-Word Interference Test,the Raven's Standard Progressive Matrices (Part A),and the WAIS Digit Span Test.Results The NLCA had high inter-rater agreement (Cronbach's α coefficient 0.836),reliability among the assessors 0.895-0.953,test-retest reliability 0.863-0.952 at at a 2-6 week interval.The years of education was significantly correlated with NLCA (r =0.852,r ＜ 0.01).When the Montreal Cognitive Assessment was used as diagnostic criteria,the area under the receiver operating characteristic curve was 0.899 (95％ confidence interval 0.827-0.972).When cutoff at 70,the NLCA had had high sensitivity,specificity,positive predictive value and negative predictive value in the identification of patients with mild cognitive impairment.Conclusions The NLCA has good reliability and validity.It is an effective cognitive function assessment that meets the basic requirements of the neuropsychological tests.

Objective To analyze the characteristics of the non-language-based cognitive function in patients with aphasia after stroke.Methods Thirty patients of the first infarction with aphasia and thirty stroke patients without aphasia were recruited.The aphasia deficits in patients were evaluated using the Aphasia Battery of Chinese(ABC).The non-language-based cognitive assessment scale (NLCA) was applied to analyze the cognitive function in the two groups.The stroke aphasic depression questionnaire hospital version (SADQ-H)was applied to analyze the depression in the two groups.Results The score in NLCA of the aphasia group was significantly lower than that of control group((50.01± 14.01)vs(66.13±5.95),P＜0.01).There was high correlation between the total and sub-scores of NLCA and ABC in both groups(P＜0.01,P＜0.05).The score in SADQ-H of the aphasia group was significantly higher than that of control group((21.83±7.37)vs(16.13±5.84),P＜0.01).The total score for the aphasia patients shown by the SADQ-H was negatively related with the total score of the NLCA(r=-0.468,P＜0.05).Conclusion There is non-language-based cognitive dysfunction in patients with aphasia after stroke,and the severity of depression affects cognitive function.

Objective To investigate the expressions of TK1 (thymidine kinase 1) in PHC (primary hepatic carcinoma). Methods TK1 and AFP in serum of 33 cases of PHC (primary hepatic carcinoma), 38 cases of hepatic cirrhosis,36 cases of hepatitis and 35 cases of healthy people were detected by means of Western blot—enhanced chemiluminecence and electrochemiluminescence. Results The difference of TK1 level in PHC group indicated significance when compared with that in hepatic cirrhosis group , hepatitis group and control group (U value was 436.4, 352.1, 163.6, respectively, all P < 0.01). TK1 level in patients with PHC was related with differentiation (χ2 = 7.476,P < 0.05) and TNM stage (χ2 = 7.227,P < 0.05),but not with sex, age, tumor diameter, number of tumors, vascular invasion, lymph node metastasis (P > 0.05). Kaplan-Meier curve analysis revealed that PHC patients with TK1≤ 2.0 pmol/L had a significantly shortened overall survival when compared with those with TK1 > 2.0 pmol/L (χ2 = 3.954,P < 0.05). Multivariable Cox regression analysis indicated that the level of TK1 and TNM stage were the independent risk factors for patients with PHC (all P <0.05). Conclusions The detection of TK1 has a certain clinical value in the diagnosis, monitoring and evaluation of the prognosis of the PHC.

Objective To study the diagnostic value of common inflammatory markers in patients with infectious diseases. Methods One hundred sepsis patients, 100 viral infection patients,100 pulmonary tuberculosis patients and 100 gonorrhea patients were analyzed retrospectively. The contents of procalcitonin (PCT), C-reactive protein (CRP), haptoglobin (HP), ceruloplasmin (CER), α1-acid glycoprotein (α1-AAG), α1-antitrypsin (α1-AAT), white blood cell count (WBC) and erythrocyte sedimetation rate (ESR) were measured. The receiver operating characteristic curve (ROC curve), sensitivity, specificity, positive predictive value, negative predictive value, Youden's index,positive and negative likelihood ratios and total coincidence rate were calculated respectively. Results The area under the ROC curve, sensitivity, specificity, Youden's index and positive likelihood ratios,positive predictive value and total coincidence rate of PCT in sepsis patients were 0. 895, 0.84, 0.92,0.76, 10.50, 0.91 and 0.88, respectively, which were superior to CRP, HP, CER, α1-AAG, α1-AAT, WBC and ESR. Conclusions PCT is a better inflammatory reactive parameter than other parameters currently applied in practice and may serve as a rapid and sensitive test in the early stage of severe bacterial infections.

Objective To investigate the effects of preoperative selective gastric arterial chemoembolization on the tumor vasculature in patients with gastric carcinoma.Methods 40 patients with gastric cancer were divided into two groups :(1)gastric arterial influsion(GAI)group;and (2)gastric arterial chemoembolization(GAE)group.The peripheral vein plasma tumor necrosis factor(TNF ?) and thrombomodulin(TM)were determined before radiologic intervention(RI),and1d,3d after R1;and the gastric vein plasma TNF ? and TM were also determined during operation.The curative surgical resection of gastric cancer was performed between 7 and 10 days after treatment.Stomach histological alterations were observed postoperatively.Results Compared with GAI group ,the plasma TNF ? levels increased markedly at all the time points,and gastric vein plasma TM levels decreased siginificantly in GAE group (P

OBJECTIVETo analyze the neurolinguistic features of a Chinese patient with pure alexia in acute and convalescent stages.

METHODSWe assessed the reading and writing abilities of the patient with the Aphasia Battery of Chinese (ABC), the reading examination of Chinese characters (1999, Lin) and the Chinese agraphia battery (CAB).

RESULTSIn the ABC examination in the acute phase, the patient performed well in oral expression and comprehension, and the prominent linguistic abnormalities were alexia and merging agraphia; in the convalescent phase, the recovery of alexia was better than that of agraphia. In reading examination of Chinese characters, shape errors were the main reading disorders in the acute phase with a few semantic errors, regularization errors and mistakes in pronunciation, but only shape errors reappeared in the recovery period. CAB examination showed impairment of writing for pictures and dictation abilities in the recovery period but recovery of other writing abilities. The writing disorder was manifested as aphasic agraphia, with obvious dysorthography and lexical errors; the patient was capable of spontaneous writing only after spontaneous speech, and was able to read the written words.

CONCLUSIONThe linguistic components of the Chinese patient with pure alexia showed different patterns of damage and recovery, suggesting the difference in their respective neuropsychological pathways.

Objective To explore the clinical characteristics and related factors of cognitive impairment in elderly post-stroke aphasia (PSA) patients.Methods Eighty-two patients after stroke,admitted to our hospitals between April 2013 and November 2014,were enrolled in this study,including 62 patients with aphasia.Based on the criteria for age segmentation from World population ageing 2009 [6],these patients were divided into 3 groups with "age=60 years":PSA patients older than 60 years as experimental group (n=30),PSA patients younger than 60 years as control group Ⅰ (n=32),and non-aphasia patients older than 60 years as control group Ⅱ (n=20).The clinical data,NIHSS scores and MRI/CT results of these patients were recorded;and the assessments of language ability,post-stroke depression (PSD) and nonlinguistic cognition were performed;classification of aphasia severity of the patients was performed by Boston diagnostic aphasia examination (BDAE).Multivariate regression analysis was performed to examine relative factors of cognitive impairment in PSA patients.Results (1) Nonlinguistic cognitive scores were significantly correlated with BDAE scores (β=0.637,P=0.000),age(β=-0.392,P=0.000),SADQ-H scores(β=-0.176,P=0.035) and cortical lesions(β=-0.150,P=0.049).(2) As compared with patients of control group Ⅰ,patients of experimental group were more likely showed deficits in most nonlinguistic cognitive domains except for abstract reasoning;and the cognitive function scale scores were significantly decreased (P＜0.05);patients of experimental group had significantly increased number of nonlinguistic cognitive domains and significantly decreased cognitive function scale scores,except for visual perception and construction scores as compared with patients of control group Ⅱ (P＜0.05).(3) The prevalence of abstract reasoning impairment was significantly lower as compared with that of visual memory,attention and executive functioning in patients of experimental group (P＜0.05).(4) The frequency and severity of impaired cognitive domains in the experiment group were significantly higher than those in the control group Ⅰ and Ⅱ (P＜0.05).Conclusions Nonlinguistic cognitive impairment is extremely common in elderly PSA patients.The aphasia severity and age are most associated with cognitive impairment in PSA patients,post-stroke depression and cortical lesions also have an effect on cognitive scores.

Objective:To investigate the distribution and morphological characteristics of brain magnetic resonance imaging (MRI) lesions in patients with myelin oligodendrocyte glycoprotein (MOG) antibody related demyelinating diseases and aquaporin-4 (AQP4) antibody positive neuromyelitis optica spectrum disorders (NMOSD) and their clinical value in early diagnosis.Methods:A total of 35 patients with MOG antibody related demyelinating diseases [20 males and 15 females; aged 31 (25, 43) years] and 36 patients with AQP4 antibody positive NMOSD [3 males and 33 females; aged 42 (29, 54) years] were collected retrospectively from September 2018 to June 2021 in Chenzhou First People′s Hospital and the Affiliated Hospital of Qingdao University which were classified as MOG group and AQP4 positive group respectively. All patients underwent routine cranial MRI scanning before treatment and the location, shape and quantity of intracranial lesions were recorded. Wilcoxon rank sum test was used to compare the number of different types of lesions between the two groups. Logistic regression analysis was used to evaluate the significance of different lesions for the two diseases.Results:There were 7 types of lesions with significant differences in different parts and shapes. Stepwise Logistic regression showed that cortical and juxtacortical lesions ( OR=21.91, 95% CI 3.09-61.69, P<0.05) and infratentorial peripheral white matter lesions ( OR=10.48, 95% CI 2.00-18.89, P<0.05) were the most important risk factors in the MOG group. The incidence of cortical and juxtacortical lesions in the MOG group was 51.4% (18/35), which was higher than that in the AQP4 positive group (2.8%, 1/36; χ2=19.02, P<0.01). The incidence of infratentorial peripheral white matter lesions in the MOG group was 31.4% (11/35), which was higher than that in the AQP4 positive group (5.6%, 2/36; χ2=6.31, P<0.05). Receiver operating characteristic (ROC) curve showed that peripheral lesions [including 6 types of lesions such as supratentorial soft meningitis, cortical encephalitis, cortical and juxtacortical lesions, infratentorial soft meningitis, infratentorial soft meningeal demyelination and infratentorial peripheral lesions, area under curve (AUC)=0.93] were more important than cortical and juxtacortical lesions (AUC=0.75) and central lesions (supratentorial paraventricular white matter lesions, diencephalon, infratentorial paraventricular lesions,AUC=0.64), which had higher diagnostic efficiency. Conclusions:The incidence of intracranial lesions in MOG antibody related demyelinating disease was higher than that in AQP4 positive NMOSD, and the distribution and morphology of intracranial MRI lesions in the two diseases had their characteristic manifestations. Identifying the distribution patterns of peripheral lesions (distributed along pia mater) and central lesions (distributed along ependyma) had a certain reference significance for distinguishing the two groups of diseases.