Objective To compare the dose distribution between three-dimensional conformal radiotherapy(3D-CRT) and intensity-modulated radiotherapy (IMRT) in treating locally advanced pancreatic cancer,and report the efficacy of IMRT combined with regional chemotherapy using gemcitabine (GEM).Methods Ten patients with locally advanced pancreatic cancer were enrolled in this study.3D-CRT and IMRT plans were designed for each patient.The dose distributions of target volume and normal tissues were analyzed using the dose volume histogram (DVH).Twenty-five locally advanced pancreatic cancers patients who were treated by IMRT combined with regional chemotherapy using gemcitabine (combined group) were retrospective analyzed,as well as 25 hospitalized patients of the same period who were treated by regional chemotherapy using gemcitabine alone (chemotherapy alone group).The therapeutic efficacy and adverse events were compared between two groups.Results IMRT plans decrease the mean dose and volume of duodenum,liver,stomach,both kidney and small bowel that received highdose irradiation.The 1-,2-year survival rate of the combined group and chemotherapy alone group was 60％,28％ and 36％,12％.The median survival time of two groups was 15 and 10 months,respectively (x2 =4.16,P ＜0.05).The total response rate of the combined group and the chemotherapy alone group was 64％ and 32％,respectively (x2 =5.13,P ＜ 0.05).The upper gastrointestinal side-effect rate of the combined group was higher than that of the chemotherapy alone group(Z =-2.354,P ＜ 0.05).There was no statistic significance in hematologic toxicity,liver and renal functional damage between the two groups.Conclusions Compared with 3D-CRT plan,IMRT plan could reduce the dose of organ at risks.IMRT combined with regional chemotherapy using gemcitabine could significantly improve the survival rate of patients with locally advanced pancreatic cancer with mild adverse events.

Objective To explore the correlation between apparent diffusion coefficient(ADC)and neonatal behavioral neurological assessment(NBNA) of newborns with hypoxic ischemic encephalopathy (HIE),and to evaluate ADC in the early diagnosis of HIE from the imaging perspective. Method One hundred and nine neonates aged 0—7 days with HIE underwent conventional MRI and DWI. According to HIE grading standards, there were 43 neonates in the mild group, 38 in the moderate group, 28 in the severe group, and meanwhile 24 normal neonates with the same ages were selected as the control group. All cases were assessed with NBNA, and ADC values of bilateral frontal white matter, parietal white matter, periventricular white matter, posterior limb of the internal capsule(PLIC), ventral lateral nucleus of thalamus, lenticular nucleus, splenium of the corpus callosum, brainstem were measured. ADC values of different groups were compared by analysis of variance, and the correlations between ADC values of all ROIs and NBNA were analyzed by Pearson correlation. Results Except lenticular nucleus and the brainstem, ADC values of frontal white matter, parietal white matter, periventricular white matter and PLIC, ventral lateral nucleus of thalamus, splenium of the corpus callosum were decreased in the mild, moderate, severe group. In the mild, moderate, and severe group, the ADC values of frontal deep white matter were(1.82± 0.33)× 10-3,(1.77 ± 0.34)× 10-3 and(1.62 ± 0.31)× 10-3 mm2/s,while they were(1.81 ± 0.34)× 10-3,(1.79 ± 0.27)× 10-3 and(1.72 ± 0.31)× 10-3 mm2/s for the parietal deep white matter,(1.27 ± 0.15)× 10-3,(1.23 ± 0.12)× 10-3 and(1.15 ± 0.17)× 10-3 mm2/s for the periventricular white matter,(1.08 ± 0.09)× 10-3,(0.97 ± 0.07)×10-3 and(0.84±0.06)×10-3 mm2/s for the PLIC,(1.13±0.07)×10-3 ,(1.08±0.13)×10-3 and(1.00± 0.13)× 10-3 mm2/s for the ventral lateral nucleus of thalamus,(1.27 ± 0.22)× 10-3,(1.18 ± 0.16)× 10-3 and (1.00 ± 0.23)× 10-3 mm2/s for the splenium of the corpus callosum. There were statistically significant differences between the 3 groups (F=61.27,16.27, 23.26, 72.70, 26.73, 66.09,all P<0.05). In the mild, modreate and severe group, NBNA were(36.8 ± 1.4) in the mild group,(33.5 ± 1.6) in the moderate,and (29.3 ± 2.6) in the severe group. There was positive correlation between ADC values of frontal white matter, parietal white matter, periventricular white matter and PLIC, ventral lateral nucleus of thalamus, splenium of the corpus callosum and NBNA scores(r=0.60,0.49,0.54,0.67,0.56,0.65,all P<0.05). Conclusions There are correlations between ADC values of the related ROIs of HIE and NBNA scores. Combining two aspects might diagnose the brain injury of HIE more accurately and objectively.

Objective To explore the relationship between the relevant factors and prostate cancer among Hui and Han populations.Methods The study involved 267 prostate cancer patients as cases ( 214 cases from Han population and 53 cases from Hui population ) and 534 prostatic hyperplasia patients as controls ( 428 cases from Han population and 106 cases from Hui population ) . All the patients were collected from the General Hospital of Ningxia Medical University during January of 2007 to September of 2013.The level of fasting blood glucose ( FBG) , total cholesterol ( TC) , triglyceride ( TG) , total prostate specific antigen (T-PSA), free prostate specific antigen (F-PSA) and free/total prostate specific antigen(F/T-PSA) were collected from the clinical medical records of the patients.Data were analyzed by the conditional logistic regression method, and attributable risk proportion ( ARP ) was calculated.Results In Hui population, the risk of prostate cancer for drinkers was 20.48 times higher than the non-drinkers (35.8%(19/53) to 5.7%(6/106), OR=20.48, 95%CI: 4.95-84.66) .The high level of F-PSA significantly increased the risk of prostate cancer for Hui group ( 83.0%( 44/53 ) to 55.7%( 59/106 ) , OR=4.27, 95%CI:1.18-15.43).In contrast, the high TG level decreased the risk of prostate cancer for Hui group (18.9%(10/53) to 20.8%(22/106), OR=0.24, 95%CI:0.07-0.83).In Han population, the risk of prostate cancer for smokers was 1.89 times higher than the non-smokers (55.1%(118/214) to 39.7%(170/428), OR=1.89,95%CI:1.28-2.78).Either high level of T-PSA or F-PSA increased the risk of prostate cancer for Han group (86.4%(185/214) to 53.7%(230/428),OR=2.34,95%CI:1.22-4.52;85.5%(183/214) to 56.1%(240/428), OR=2.43, 95%CI:1.29-4.59).However, the high TG level or high ratio of F/T-PSA decreased the risk of prostate cancer ( 15.4%( 33/214 ) to 18.7%( 80/428), OR=0.59, 95%CI:0.36-0.98;53.3%(114/214) to 73.4%(314/428), OR=0.53, 95%CI:0.36-0.78).The APRs of drinking and high level of F-PSA in Han populations were 66.6%,62.4%in Hui populations ,and the APRs of smoking and high level of T-PSA, high level of F-PSA were 33.8%,71.3%, 67.3% in Han populations.Conclusion Both drinking and high level of F-PSA might be the risky factors of prostate cancer while the high TG level might be protective factor for Hui group. However, for Han population, smoking, high T-PSA level, and high F-PSA level might be risky factors for prostate cancer while the high TG level and high ratio of F/T-PSA might be protective factors.In summary, the clinical relevant factors of prostate cancer may play different roles between Hui and Han populations in Ningxia region.

Objective To explore the relationship between the relevant factors and prostate cancer among Hui and Han populations.Methods The study involved 267 prostate cancer patients as cases ( 214 cases from Han population and 53 cases from Hui population ) and 534 prostatic hyperplasia patients as controls ( 428 cases from Han population and 106 cases from Hui population ) . All the patients were collected from the General Hospital of Ningxia Medical University during January of 2007 to September of 2013.The level of fasting blood glucose ( FBG) , total cholesterol ( TC) , triglyceride ( TG) , total prostate specific antigen (T-PSA), free prostate specific antigen (F-PSA) and free/total prostate specific antigen(F/T-PSA) were collected from the clinical medical records of the patients.Data were analyzed by the conditional logistic regression method, and attributable risk proportion ( ARP ) was calculated.Results In Hui population, the risk of prostate cancer for drinkers was 20.48 times higher than the non-drinkers (35.8%(19/53) to 5.7%(6/106), OR=20.48, 95%CI: 4.95-84.66) .The high level of F-PSA significantly increased the risk of prostate cancer for Hui group ( 83.0%( 44/53 ) to 55.7%( 59/106 ) , OR=4.27, 95%CI:1.18-15.43).In contrast, the high TG level decreased the risk of prostate cancer for Hui group (18.9%(10/53) to 20.8%(22/106), OR=0.24, 95%CI:0.07-0.83).In Han population, the risk of prostate cancer for smokers was 1.89 times higher than the non-smokers (55.1%(118/214) to 39.7%(170/428), OR=1.89,95%CI:1.28-2.78).Either high level of T-PSA or F-PSA increased the risk of prostate cancer for Han group (86.4%(185/214) to 53.7%(230/428),OR=2.34,95%CI:1.22-4.52;85.5%(183/214) to 56.1%(240/428), OR=2.43, 95%CI:1.29-4.59).However, the high TG level or high ratio of F/T-PSA decreased the risk of prostate cancer ( 15.4%( 33/214 ) to 18.7%( 80/428), OR=0.59, 95%CI:0.36-0.98;53.3%(114/214) to 73.4%(314/428), OR=0.53, 95%CI:0.36-0.78).The APRs of drinking and high level of F-PSA in Han populations were 66.6%,62.4%in Hui populations ,and the APRs of smoking and high level of T-PSA, high level of F-PSA were 33.8%,71.3%, 67.3% in Han populations.Conclusion Both drinking and high level of F-PSA might be the risky factors of prostate cancer while the high TG level might be protective factor for Hui group. However, for Han population, smoking, high T-PSA level, and high F-PSA level might be risky factors for prostate cancer while the high TG level and high ratio of F/T-PSA might be protective factors.In summary, the clinical relevant factors of prostate cancer may play different roles between Hui and Han populations in Ningxia region.

Objective:To evaluate the effect of activation of splenic plasmacytoid dendritic cells (pDCs) on myocardial ischemia-reperfusion (I/R) injury in mice.Methods:The experiment was performed in two parts. Animal experiment Thirty-six SPF healthy male C57BL/6J mice, aged 10 weeks, weighing 22-27 g, were assigned to 3 groups ( n=12 each) using a random number table method: sham operation group (Sham group), myocardial ischemia group (MI group) and myocardial I/R group (MI/R group). The myocardial ischemia was induced by occluding the left anterior descending coronary artery for 40 min in MI group, while the model of myocardial I/R was established by occlusion of the left anterior descending coronary artery for 40 min followed by 1-h reperfusion in MI/R group. Following successful preparation of the model, 3 animals from each group were randomly selected, and their hearts were removed for determination of myocardial infarct size through a combination of TTC and methylene blue double staining. Another 3 animals from each group were randomly selected, and their hearts were removed for examination of pathological changes of myocardial tissues using HE staining. Blood samples were collected from the abdominal aorta of 6 mice left in each group for determination of plasma interferon alpha (IFN-α) concentrations by enzyme-linked immunosorbent assay. Then the animals were sacrificed and hearts were harvested for collection of cardiac perfusate (CP). Cell experiment Twelve SPF healthy male C57BL/6J mice, aged 10 weeks, weighing 22-27 g, were selected and the splenic pDCs were isolated using anti-mPDCA-1 MicroBeads according to the manufacturer′s instructions (with a positivity rate of >85% for the isolated cells). The cells were divided into 4 groups: group pDCs stimulated by CP in Sham group (pDCs+ CP-Sham group), group pDCs stimulated by CP in MI group (pDCs+ CP-MI group), group pDCs stimulated by CP in MI/R group (pDCs+ CP-MI/R group) and pDCs stimulated by PBS group (pDCs+ PBS group). The CP in Sham, MI and MI/R groups and PBS were used to induce and culture pDCs for 8 h. Flow cytometry was employed to detect the expression of CD45 and co-stimulatory molecules CD80, CD86 and Major Histocompatibility Complex Ⅱ (MHC Ⅱ) on the surface of pDCs. The levels of IFN-α in the cell culture supernatant were determined using enzyme-linked immunosorbent assay. Results:Animal experiments Compared with Sham group and MI group, the percentage of myocardial infarct size was significantly increased, the concentrations of plasma IFN-α were increased ( P<0.05), and cardiomyocytes displayed evident vacuolar degeneration, severe myocardial fiber rupture, and infiltration of a substantial number of inflammatory cells in MI/R group. There was no significant difference in each parameter between Sham group and MI group ( P>0.05). Cell experiment Compared with pDCs+ CP-Sham group, the expression of CD80, CD86 and MHCⅡ was significantly up-regulated in pDCs+ CP-MI group ( P<0.05), and no significant change was found in the aforementioned parameters in pDCs+ CP-MI/R group ( P>0.05). The expression of aforementioned parameters was significantly up-regulated in pDCs+ CP-MI group as compared with pDCs+ CP-MI/R group ( P<0.05). Compared with pDCs+ CP-Sham group and pDCs+ CP-MI/R group, the concentrations of IFN-α in the cell culture supernatant were significantly increased in pDCs+ CP-MI group ( P<0.05). There was no statistically significant difference in the concentrations of IFN-α between pDCs+ CP-MI/R group and pDCs+ CP-Sham group ( P>0.05). Conclusions:The mechanism underlying myocardial I/R injury may be related to activation of splenic pDCs leading to the production of IFN-α following myocardial ischemia in mice.

OBJECTIVETo explore the relationship between the relevant factors and prostate cancer among Hui and Han populations.

METHODSThe study involved 267 prostate cancer patients as cases (214 cases from Han population and 53 cases from Hui population) and 534 prostatic hyperplasia patients as controls (428 cases from Han population and 106 cases from Hui population). All the patients were collected from the General Hospital of Ningxia Medical University during January of 2007 to September of 2013. The level of fasting blood glucose (FBG), total cholesterol (TC), triglyceride (TG), total prostate specific antigen (T-PSA), free prostate specific antigen (F-PSA) and free/total prostate specific antigen (F/T-PSA) were collected from the clinical medical records of the patients. Data were analyzed by the conditional logistic regression method, and attributable risk proportion(ARP) was calculated.

RESULTSIn Hui population, the risk of prostate cancer for drinkers was 20.48 times higher than the non-drinkers (35.8% (19/53) to 5.7% (6/106), OR = 20.48, 95% CI: 4.95-84.66). The high level of F-PSA significantly increased the risk of prostate cancer for Hui group (83.0% (44/53) to 55.7% (59/106), OR = 4.27, 95%CI: 1.18-15.43). In contrast, the high TG level decreased the risk of prostate cancer for Hui group (18.9% (10/53) to 20.8% (22/106), OR = 0.24, 95% CI: 0.07-0.83). In Han population, the risk of prostate cancer for smokers was 1.89 times higher than the non-smokers (55.1% (118/214) to 39.7% (170/428), OR = 1.89, 95% CI: 1.28-2.78). Either high level of T-PSA or F-PSA increased the risk of prostate cancer for Han group (86.4% (185/214) to 53.7% (230/428),OR = 2.34, 95%CI:1.22-4.52;85.5% (183/214) to 56.1% (240/428), OR = 2.43, 95% CI:1.29-4.59). However, the high TG level or high ratio of F/T-PSA decreased the risk of prostate cancer (15.4% (33/214) to 18.7% (80/428), OR = 0.59, 95% CI:0.36-0.98; 53.3% (114/214) to 73.4% (314/428), OR = 0.53, 95% CI:0.36-0.78). The APRs of drinking and high level of F-PSA in Han populations were 66.6%, 62.4% in Hui populations , and the APRs of smoking and high level of T-PSA, high level of F-PSA were 33.8%, 71.3%, 67.3% in Han populations.

CONCLUSIONBoth drinking and high level of F-PSA might be the risky factors of prostate cancer while the high TG level might be protective factor for Hui group. However, for Han population, smoking, high T-PSA level, and high F-PSA level might be risky factors for prostate cancer while the high TG level and high ratio of F/T-PSA might be protective factors. In summary, the clinical relevant factors of prostate cancer may play different roles between Hui and Han populations in Ningxia region.

Alcoholic Beverages ; Biopsy ; Case-Control Studies ; China ; Humans ; Male ; Prostate-Specific Antigen ; Prostatic Neoplasms ; ethnology ; Risk Factors ; Smoking ; Triglycerides

OBJECTIVE To evaluate the effectiveness and safety of Panlongqi tablets in the treatment of fractures based on real-world research. METHODS From September 2021 to September 2023， fracture patients admitted to 33 medical institutions were collected retrospectively. Patients who received conventional treatment were divided into control group （n＝3 750）， and patients who received combination of Panlongqi tablets on the basis of conventional treatment were divided into observation group （n＝ 3 706）. Self-reported indicators of patients were collected through telephone follow-up at 0， 4， 7 and 14 days after treatment. The improvement values of pain score， swelling score and health utility value， as well as effective rate and adverse drug reactions were compared between 2 groups. The propensity matching score （PSM） method was adopted to perform baseline matching on patient’s age， gender， fracture site， fracture severity， surgical type， type of hospital， and other indicators. Statistical analysis was performed on each therapeutic effect indicator. RESULTS After PSM， a total of 6 425 patients were included， of which 3 055 were in the observation group and 3 370 were in the control group. After 14 days of treatment， the observation group showed significant improvement in pain score （4.768 vs. 4.353）， swelling fangshiyuan2008@126.com grading score （2.979 vs. 2.391）， and life quality utility value （0.430 vs. 0.363）， as well as effective rate （87.20% vs.75.99%） compared to the control group （P＜0.05）. The results of subgroup analyses conducted by gender， age， hospital type， and fracture site were consistent with the aforementioned results. In terms of safety， the observation group had no serious adverse reactions， with a total of 29 cases of mild adverse reactions such as dizziness， stomach pain， and allergies， with an incidence rate of 0.78%. CONCLUSIONS Panlongqi tablets combined with conventional treatment are significantly better than conventional treatment in improving pain， swelling， quality of life， and effective rate in patients with fractures， and have good safety.