1.MicroRNA and tumor metastasis
Cancer Research and Clinic 2009;21(1):65-67
MicroRNA (miRNA) is a kind of small non-protein coding RNA and can negatively regulate the expression of target genes at the transcriptional level. In tumors miRNA can act as oncogenes and tumor suppressor genes, and its deregulation in tumors may be a possible reason for tumor progression. Several studies have established the potential usefulness of miRNA as therapeutic targets for tumor metastasis.
2.Small interfering RNA in gastrointestinal cancer
Feng JU ; Junbo ZUO ; Jiazeng XIA
Journal of International Oncology 2012;39(7):538-540
SiRNA comes from double-stranded RNA,which is processed into a small molecular fragment by Dicer.21-25nt siRNA,as the key effector molecules to the RNAi process,can inhibit gene expression with high specificity and high efficiency in mammalian cells.Currently,RNAi has been widely applied in a variety of cancer.RNAi has many active research explorations of the tumor development,metastasis and treatment in gastrointestinal cancer.
3.Expression of Ezrin and AnnexinⅡin gallbladder carcinoma
Yigang CHEN ; Jiazeng XIA ; Su QU
The Journal of Practical Medicine 2016;32(19):3147-3150
Objective To investigate the expression levels of Ezrin and AnnexinⅡ in gallbladder carci-noma and their association with clinicopathologic parameters and metastasis potential. Methods The tissue mi-croarray consisted of 59 gallbladder carcinoma tissues and 6 normal gallbladder tissues were examined for the ex-pression of Ezrin and AnnexinⅡusing immunohistochemistry technique. The expression of Ezrin and AnnexinⅡin 20 cases of fresh gallbladder carcinoma and 6 cases of normal gallbladder were measured with western blot. Results The expression of Ezrin and AnnexinⅡ were higher in the gallbladder cancer than those in the normal gallbladder tissue. The positive rate of Ezrin and AnnexinⅡ were 47.5% and 50.8% respectively. The expression of Ezrin was significantly correlated with live metastasis , lymph node metastasis and Nevin stages. The expression of AnnexinⅡwas significantly correlated with live metastasis , differentiation levels and Nevin stages. The expres-sion of Ezrin was correlated with AnnexinⅡ. Results of western blot suggested that Ezrin and Annexin II were highly expressed in gallbladder carcinoma tissues. The high expression of Ezrin and Annexin is closely related with liver invasion. Conclusion Measurement of the expression of Annexin and Ezrin II have important clinical significances to evaluate the malignant biological behavior of gallbladder carcinoma.
4. Application value of ω-3 fish oil fat emulsion in the parenteral nutritional support treatment following radical gastrectomy for gastric cancer
Ting SHAN ; Yigang CHEN ; Bo HONG ; Chen GU ; Hong ZHOU ; Jiazeng XIA
Chinese Journal of Digestive Surgery 2019;18(10):960-965
Objective:
To investigate the application value of ω-3 fish oil fat emulsion in the parenteral nutritional support treatment following radical gastrectomy for gastric cancer.
Methods:
The retrospective cohort study was conducted. The clinical data of 60 patients who underwent radical gastrectomy for gastric cancer in Nanjing Medical University Affiliated Wuxi Second Hospital between January 2018 and December 2018 were collected. There were 37 males and 23 females, aged from 28 to 78 years, with an average age of 64 years. Thirty patients who received parenteral nutrition containing 100 mL of ω-3 fish oil fat emulsion after radical gastrectomy and 30 patients who received parenteral nutrition containing routine fat emulsion after radical gastrectomy were allocated into experimental group and control group, respectively. Observation indicators: (1) nutritional indicators in the perioperative period; (2) inflammatory indicators in the perioperative period; (3) immune indicators in the perioperative period; (4) postoperative complications. Measurement data with normal distribution were represented as
5.The efficacy and safety of morinidazole combined with appendectomy in treating purulent or gangrenous appendicitis: a randomized, controlled, double-blind, multi-center clinical trial
Yun TANG ; Mingqing TONG ; Hao YU ; Yanping LUO ; Mingzhang LI ; Yongkuan CAO ; Mingfang QIN ; Lie WANG ; Xiaoqiang WANG ; Bo PENG ; Yong YANG ; Shuguang HAN ; Chungen XING ; Bing CAI ; Jianming HUANG ; Jiazeng XIA ; Bainan LYU ; Liang XU ; Jilin YI ; Dechun LI ; Guoqing LIAO ; Xiaofeng ZHEN ; Daogui YANG ; Zhongcheng HUANG ; Haibo WANG
Chinese Journal of General Surgery 2017;32(8):678-682
Objective To assess the efficacy and safety of morinidazole combined with appendectomy in treating purulent or gangrenous appendicitis.Methods Double-blind randomized controlled multicenter clinical trial was designed and conducted.Totally 437 patients were included,219 in the control group and 218 in the experimental group.Cases of purulent or gangrenous appendicitis were enrolled and assigned to each of the two groups.The control group received ornidazole injection for 5 to 7 days while the experimental group received morinidazole injection.Both groups underwent appendectomy.Clinical response,micrombiological outcomes,overall response were evaluated.Adverse events and side effects were recorded.Results No significant difference was observed between the two groups regarding the clinical healing rate at 5-10 days after medicine withdrawal,anaerobia clearance and overall healing rates.Adverse events occurred in 140 patients (32.1%).Incidence of adverse events in the control group and the experimental group was 34.7% and 29.4%,respectively (P > 0.05).The overall incidence of side effects was 15.1% (66 cases).Side effects were less seen in the experimental group compared with that in the control group (11.5% vs.18.7%,P < 0.05).The most frequent side effects were aminotransferase rising,thrombocytosis,nausea,vomiting and electrocardiographic abnormality.Conclusions The effect of morinidazole plus operation was comparable with ornidazole in treating purulent or gangrenous appendicitis.The safety of morinidazole is better than ornidazole.