1.TLC Identification and Total Flavonoids Determination in Herb Sarcandra from Different Producing Areas
Jiayun TONG ; Wei LI ; Chaomei PAN
Traditional Chinese Drug Research & Clinical Pharmacology 1993;0(04):-
Objective To explore the whole quality control method for lactones and flavonoids in the raw material of Herb Sarcandra.Methods TLC identification of ethyl acetate extracts and spectrophotometry determination of total flavonoids had been conducted.Results Chemical compositions extracted by ethyl acetate and total flavonoids content varied greatly in the raw materials of Herb Sarcandra from different producing areas.Conclusion TLC identification combined with total flavonoids determination can be used for the quality control of the medicinal material of Herb Sarcandra.
2.Histocytological Observation of Somatic Embryogenesis in In-Vitro Cultured Dendrobium candidum wall. Ex Lindl.
Chaomei PAN ; Jiayun TONG ; Danxia LIU ; Aiqun HU ; Shuqing YANG
Journal of Guangzhou University of Traditional Chinese Medicine 2001;0(01):-
Objective To observe the somatic embryogenesis in in-vitro cultured Dendrobium candidum wall.Ex Lindl.(DCWL),and to supply evidence for its rapid propagation and germplasm preservation.Methods Protocorm-like bodies and callus of DCWL subcultured for 30 days was used as the explants,N6 was used as the basic culture with phytohormone added,and fungal extracts as the elicitor.Protocorm-like bodies and callus of DCWL were used for induction culture.Results Somatic embryogenesis in in-vitro cultured DCWL derived from the epithelial cells or inner cells of callus of DCWL.Conclusion A large amount of buds can be obtained by the induction and culture of protocorm-like bodies and callus of DCWL.
3.Quality Evaluation of Cortex Ilicis Rotundae Medicinal Materials
Yanqin OUYANG ; Ping YAN ; Zhuojun CHEN ; Wenhua LIN ; Jiayun TONG ; Ruoting ZHAN
Journal of Guangzhou University of Traditional Chinese Medicine 2015;32(6):1075-1081,1152
Objective To investigate and evaluate the quality of Cortex Ilicis Rotundae medicinal materials in Chinese herbal medicine market. Methods Twenty-two batches of Cortex Ilicis Rotundae commercial medicinal materials were identified and analyzed by characteristic identification, microscopic identification, thin layer chromatography ( TLC) and extractives determination, and the contents of syringin and pedunculoside were detected by high performance liquid chromatography (HPLC) according to the Chinese Pharmacopoeia published in 2010. And then the quality of the medicinal material of Cortex Ilicis Rotundae was evaluated comprehensively. Results Of the 22 batches of Cortex Ilicis Rotundae medicinal materials, 2 batches were adulterated with fake Cortex Ilicis Rotundae, one batch was inferior and was mixed with counterfeits, and the quality of 12 batches was also poor. Conclusion At present, the quality of Cortex Ilicis Rotundae medicinal materials in Chinese herbal medicine market varies greatly, and adulterants and the inferior are common.
4.Identifying potential anti-COVID-19 pharmacological components of traditional Chinese medicine Lianhuaqingwen capsule based on human exposure and ACE2 biochromatography screening.
Xiaofei CHEN ; Yunlong WU ; Chun CHEN ; Yanqiu GU ; Chunyan ZHU ; Suping WANG ; Jiayun CHEN ; Lei ZHANG ; Lei LV ; Guoqing ZHANG ; Yongfang YUAN ; Yifeng CHAI ; Mingshe ZHU ; Caisheng WU
Acta Pharmaceutica Sinica B 2021;11(1):222-236
Lianhuaqingwen (LHQW) capsule, a herb medicine product, has been clinically proved to be effective in coronavirus disease 2019 (COVID-19) pneumonia treatment. However, human exposure to LHQW components and their pharmacological effects remain largely unknown. Hence, this study aimed to determine human exposure to LHQW components and their anti-COVID-19 pharmacological activities. Analysis of LHQW component profiles in human plasma and urine after repeated therapeutic dosing was conducted using a combination of HRMS and an untargeted data-mining approach, leading to detection of 132 LHQW prototype and metabolite components, which were absorbed