Objective]To summarize Professor Wang’s clinical thoughts and experience of herb pairs in treating“cough caused by wind”. [Methods]To collect and analyse the theory of Wang’s experience of herb pairs in treating“cough caused by wind”by studying from him for a long time. [Results] Professor considered that“cough caused by wind”is a kind of disease characterized by nature of “wind”, which is mainly cough, with wind syndrome unique performance. It is similar to airway hyperresponsiveness diseases,including modern medicine on the infection cough, cough variant asthma, gastroesophageal reflux cough, upper airway cough syndrome, eosinophilic bronchitis, allergic cough, etc, which should be treated by the way of “dispelling wind ventilating lung and relieving cough ”. According to different wind cough sydromes, choose different herb couples.“Feixingcao-Zicao-Xiancao”,“Jiegeng-Shegan”,“Zhipipaye -Zhiziwan -Zhidonghua”,“Zhimahuang -Suye”,“Xinreng -Zhebei”,“Shengbaishao -Shenggancao”are the most commonly used herb pairs in practice.[Conclusion] Professor’s experience of herb pairs in treating“cough caused by wind”is well worth learning for it always gets outstanding effect.

Objective To investigate the distribution and clinical significance of D‐Dimer positive patients .Methods 1 003 D‐di‐mer positive patients were enrolled in the study ,which were measured by latex enhanced immune turbidimetry .Results The total positive rate of ICU ,cardiology ,respiratory medicine ,orthopedics ,general surgery ,liver disease ,neurosurgery ,obstetrics and gyne‐cology ,oncology departments was 44 .1% .The numbers of D‐dimer positive patients with diffuse intravascular coagulation ,deep vein thrombosis ,pulmonary embolism ,heart cerebrovascular disease ,liver disease ,malignant tumor were 86 ,34 ,26 ,24 ,18 and 12 , respectively .Conclusion The determination of plasma D‐dimer could be used in thrombotic disease prevention and monitoring .

From recent collection of 40 male adult cadavers from Chungking, the authors caIculated the coefficient of correlation between the average lengths of long bones of both sides and the stature measured from the cadavers. The figures range from 0.719～0.849, which denote a rather high degree of correlation. Hence, 10 regression formulae were computed. After a thorough analysis of the equations, it indicates that the relationship between stature and long bones of the lower limb reveals a higher coefficient of correlation and a lower standard error of estimation than that from the upper limb. Moreover, the reconstruction of stature from the sum of the lengths of femur and tibia (or fibula) appears still more reliable than that from the femur alone. The regression formulae obtained had been compared with the only data thus far found on the North China sample (Stevenson, 1929),it exhibits that all regression lines of both sources parallel with each other (Chungking lines being placed a little bit lower), with the exception of the two humerus lines which intersect. Therefore, whether regression formulae computed from this paper can be expected to give an approximation to reality in different districts of China or not demands further checking over with figures derived from the stature estimate made in different localities by the same method.

Objective To observe the clinical effects of treating acute upper gastrointestinal bleeding(AUGIB) with freezing Zidi mixture lavage. Method 183 UGIB patients from the First Affiliated Hospital to Guanzhou University of TCM in August 2013 were randomly recruited into a control group with 91cases and a treatment group with 92 cases. The treatment group was treated with intragastric administration of freezing Zidi mixture, while the control group was treated with intragastric administration of cryohydrate and norepinephrine. The changes of clinical effects, blood pressure, hemoglobin, blood urea nitrogen, pulse and dark stool were observed and the bad emotions as anxiety, depression and fear were evaluated. Results ① On therapeutic effect, the total effective rate was 96.7% and 85.7% in the treatment group and the control group respectively, with statistical difference(χ2=2.943,P<0.05). ② On the time of symptom disappearance:the disappearing time of vomiting, dark stools and occult blood test in the treatment group was obviously shorter than the control group after the treatment(t=6.628、14.052、21.706,P<0.05 or 0.01). ③ On body symptom changes: after the treatment, the systolic pressure, heart rate, hemoglobin, and blood urea nitrogen [(114.3±12.1)mmHg,(78.6±11.4)/min,(98.1±10.5)g/L,(5.9±1.1)mmol/L] in the treatment group were all obviously improved than those [(106.3±11.2)mmHg, (88.7±12.1)/min,(88.2±11.7)g/L,(6.9±1.4)mmol/L] in the control group;body temperature returned to normal in both groups, and there was no statistical difference score was either in or between groups(P>0.05). ④ On changes of anxiety, depression and fear:the treatment group [the(42.5±9.4), (41.6±9.7), (26.3±5.4)for each]was obviously improved after the treatment than the control group[the score was(51.2±10.1),(50.4±11.5),(30.4±6.9)for each],P<0.05. Conclusion Freezing Zidi mixture lavage can obviously elevate systolic pressure, decrease heart rate, enhance hemoglobin, decrease blood urea nitrogen, relieve body stress reaction and other clinical symptoms. Its effects were better than intragastric administration of cryohydrate and norepinephrine in treating AUGIB.

0.05) in the negative group. However, there was a significant increase in each parameter except Rc in the positive group (P

Objective To explore the differences in mental health self-help status of empty-nesters between urban and rural areas for providing the basis and strategies in mental health self-help and promoting development of the self-help potential.Methods Inner Mongolia Chifeng district,441 cases of urban and rural empty-nesters was valuated with the mental health questionnaire.Results Urban empty-nesters were higher than the ratio of rural empty-nester on the aspects of residential satisfaction,participating in activities,talking about their problems in families,communicating health informations and seeking professional help,x2=8.193,15.930,6.500,13.089,18.857,P ＜ 0.05.Urban empty-nesters were lower than the ratio of rural empty-nester on the aspects of feeling helpless and hiding bad mood,x2=9.930,14.873,P ＜ 0.05.The awareness on mental health knowledge was in low level for both urban and rural areas(17.11％,32/187 and 9.55％,19/199).Conclusions Both urban and rural empty-nesters were in bad conditions of mental health self-help ability,especially for rural empty-nesters.The awareness rate of knowledge on mental health is in highly need.

Objective To establish a new method for monitoring aspirin response by platelet activated-release experiment.Methods The platelets in whole blood were activated by ARA,and the MPC was measured by hematology analyzer.Blood samples were drawn from five healthy volunteers for measuring MPC,LTAARA and platelet membrane CD62P expression.Blood samples were mixed thoroughly right after venipuncture. The concentration of ARA (0,0. 5,1.0,1.5,5.0 and 10. 0 mmol/L) and the time for platelet activation (5,10,20,30,40,50,60,70,80 and 90 min in 37℃ water bathe) were optimized to evaluate the stability (0,1,2 and 3 h after venipuncture) and reproducibility (MPC, LTAARA and platelet membrane CD62p were measured ten times and the CV was calculated). Platelets were mixed with acetylsalicylic acid at different concentrations in vitro in order to verify the validity for monitoring aspirin response. The percentage of CD62p positive platelets after activated by ARA was measured using flow cytometer with CD61-PerCP and CD62p-PE antibodies. The correlation between MPC and CD62P was determined. 100 patients without taking or stop taking aspirin more than 7 days and 93 patients who took aspirin at least 7 days were enrolled. Duplicate measurements of platelet function were performed using the change of MPC (ARA 0. 5 mmol/L) and LTA (ARA 0. 5 mmol/L) among two patient groups to evaluate the accuracy of the new method. Results Platelcts were completely activated by ARA at final concentration of 0. 5 mmol/L. MPC negatively correlated with platelet membrane CD62p(r=-0. 755,P<0. O1 ). MPC was stable for 30 minutes in 37 ℃ water bathe after ARA activation. The result of MPC was consistent at room temperature within 3 hours after blood collection. This method had good reproducibility. CV in batch using fresh whole blood was 1.35% and CV between batches using commercial control whole blood were 0. 71% and 0. 74%. With the concentration of acetylsalicylic acid increased (0-6. 95 μmol/L), MPC increased as CD62P decreased, which showed negative correlation (r=-0. 765 ,P <0. 01 ). The difference of MPC before and after ARA activation (ΔMPC) and MPC variance ratio of the group taking aspirin were ( 8. 2±8. 6) g/L and ( 3.4±3.6) %, and they were (37.4±10. 3 ) g/L and ( 15.7±4.0) % in control group, respectively.ΔMPC and MPC variance ratio showed significant difference between the two groups ( t=21. 522, 22. 409, all P < 0. 01 ). Area under the ROC curve for MPC variance ratio was 0. 992 with sensitivity of 96. 8% and specificity of 99.0% for monitoring the aspirin response using the cut-off of 8. 7%. MPC variance ratio had good correlation with LTAARA (r = 0. 720, P < 0. O1 ). Conclusions A new method for monitoring aspirin response by platelet activated-release experiment is established. It may replace LTAARA for routine clinical examination.

Objective To investigate the changes of T lymphocyte subsets of severe acute respiratory syndrome(SARS) patients during acute phase and recovery stage and study its clinical significance in early diagnosis of SARS.Methods T lymphocyte subsets of 40 patients of SARS and 22 cases of SARS recovery stage were detected by flow cytometry.Results Compared with normal group, T lymphocyte, Th and Ts cell counts of SARS patients decreased obviously( P =0.013, P 0.05). Conclusion The cellular immunity of SARS patients were badly damaged during acute phase. Their T lymphocyte, Th and Ts cell counts decreased obviously. This could be a guideline of early diagnosis of SARS. The counts of T lymphocyte subsets of SARS patients can return to normal after they were cured.

Objective To research on a whole blood control material for lymphocyte subset counting by flow cytometry(FCM).Methods To detect lymphocyte subset in whole blood with different preservers by flow cytometric multi-color analysis.Results whole blood control material for lymphocyte subset counting by FCM was prepared.In 2-8℃ refrigerator, the light scatter and CD45 of leukocytes of whole blood control were stable in 72 days. The cluster of lymphocyte, monocyte, neutrophil in plot were separated easily from debris. The lymphocyte subset of whole blood control can be counted by FSC/SSC or CD45/SSC gating. The variation of lymphocyte subset count was less in different preserving day. The coefficient of variation (CV) of lymphocyte subset count was less than 6.5% in our laboratory and less than 13% in external quality assessment among 56 laboratories in China.Conclusion The whole blood control prepared by us can be used for internal quality control and external quality assessment in lymphocyte subset counting by FCM, it is very important signification to ensure the quality and accuracy of lymphocyte subset count.

Objective To study the action of aspirin, ticlopidine and cilostazol in inhibiting platelet activation in vitro. Methods Three kinds of anti platelet drugs, which inhibited the expression of fibrinogen receptor (FIB R) and P selectin (CD62P) on activated platelets surface in vitro, were analyzed by tri color flow cytometry. Results Three kinds of anti platelet drugs inhibited the expression of FIB R and CD62P on ADP activating platelet membrane surface differently. Cilostazol could inhibit the expression of FIB R and CD62P of the platelets markedly. Ticlopidine could inhibit the FIB R expression markedly, but CD62P indistinctly. Aspirin could not inhibit the expression of either FIB R and CD62P on ADP activating platelets. Conclusion Cilostazol is a strong anti platelet drug as compared with aspirin and ticlopidine. It could not only inhibit FIB R expression of activated platelets, which can prevent platelet aggregation, but also decrease the release reaction and procoagulative activities of activated platelets.