AIM:To investigate whether trichostatin A ( TSA) , a new revulsant ,can induce mouse mesenchy-mal stem cells to differentiate into insulin-secreting cells and to explore the appropriate concentration of TSA .METHODS:The mesenchymal stem cell line from C57BL/6 mice was cultured in vitro and divided into 5 groups before treated with dif-ferent concentrations of TSA , ( group A:DMSO;group B~E:treated with 25 nmol/L, 50 nmol/L, 100 nmol/L and 200 nmol/L of TSA, respectively).After exposed to different cultured media for 10 d during the 2 stages, the cells were detec-ted by the following methods:the insulin-secreting cells in each group were identified by dithizone staining and the results were calculated with immunohistochemical half quantitative analysis .The insulin secreted by insulin-secreting cells in each group was identified by immunofluorescence , and the mean fluorescence intensity of insulin was compared .The content of insulin in each group was quantified by ELISA .The appropriate concentration of TSA was determined according to the above results .RESULTS:TSA treatment for 10 d promoted the mouse bone marrow mesenchymal stem cells to differenti-ate into insulin-secreting cells which produced insulin .The immunohistochemistry and immunofluorescence imaging analysis of insulin-secreting cells showed that the insulin staining positive area , positive ratio , total density of insulin expression and mean fluorescence intensity of insulin in group B were significantly higher than those in the other TSA -treated groups .When the concentrations of TSA gradually increased , the content of insulin reduced accordingly .The content of insulin in group B was significantly higher than that in the other TSA-treated groups .CONCLUSION:TSA treatment for 10 d promotes bone marrow mesenchymal stem cells from C57BL/6 mice to differentiate into insulin-secreting cells and the appropriate concen-tration of TSA is 25 nmol/L.

0.05). The BHR distribution was slight 31.9%, mild 43.5%, moderate 24.6%, no severe BHR were found in this study, showing less correlation between the percentage of predicted FEV 1 and the PD 20FEV 1-His in the elderly (r=0.277, P

Objective: To investigate the extravasate sponging and hemostatic property of chitosan hemostatic sponge on the wound of otic vein of rabbit, and its healing effect on the scald of rabbit skin, for clinical application. Methods: Lacerating the otic vein of rabbit with bistoury and promptly pressing the wound with chitosan hemostatic sponge, gelatin sponge or gauze, respectively, the hemostatic effect was observed by uncovering each 30 seconds. Spreading chitosan hemostatic sponge, gelatin sponge or gauze, respectively, on the scalds of rabbit skin, the decrustation (pulling off scab) was observed within 10 days. Results: Comparing with the group of gelatin sponge, the group of chitosan hemostatic sponge showed significant differences in bleeding time and wound healing (P

Objective To observe the frequency of interleukin-6(IL-6) gene -572C/G polymorphism and its relationship with myocardial infarction(MI) and serum lipids in Chinese Hans. Methods IL-6/gene-572C/G polymorphism was genotyped in 232 MI patients and 260 healthy adults by PCR-RFLP method. Results There were IL-6/gene-572 CC、CG and GG genotype. -572GG genotype and G allele were more frequent in patients than those in controls(P

Objective To compare the efficacy of gradient and swim-up semen preparation techniques on pregnancy rates in couples undergoing intrauterine insemination (IUI) cycles with human menopausal gonadotropin (HMG) stimulation. Methods Five hundred and seventy one cycles were devided into the swim up or the gradient technique groups for sperm preperation. The clinical pregnancy rates per cycle were evaluated. Results The clinical pregnancy rates per cycle were significantly higher in the gradient group (17.8%) than that in the swim up group (11.4%)(P < 0.05). In the subgroup of unexplained subfertile couples, the gradient group also revealed significantly higher clinical pregnancy rates per cycle (33.7%) than that in the swim up group (20.3%)(P<0.05). In couples with mild male factor subfertility, the gradient group also revealed significantly higher clinical pregnancy rates per cycle (11.6%) than that in the swim up group (7.6%)(P < 0.05). Conclusion The gradient technique significantly improves clinical outcome in IUI cycles of unexplained subfertile and male subfertile couples.

Objective To study and evaluate the MRI findings and their diagnostic value for breast carcinoma.Methods Preoperative contrast-enhanced MR imaging and mammography were performed in 12 patients who underwent surgery and were histopathologically proved with breast carcinoma.MR imaging findings were compared with clearly mammography.Results Twenty-one breast lesions in 12 cases were detected with MR imaging.Seventeen of the twenty-one lesions were detected with mammography.Contrast-enhanced MR imaging was superior to mammography to demonstrate lesions in these dense breasts.It could clearly indicate the extension of posterior breast carcinoma into the pectoralis major muscle and the metastases of parasternal lymphatic node,while it was difficult to evaluate with mammography.The "rim enhancement sign" might be valuable to diagnose breast carcinoma.Conclusion The sensitivity and accuracy of MR imaging is superior to that of mammography.Contrast-enhanced imaging of the breast is helpful not only in early diagnosis of breast carcinoma but also in staging breast carcinoma and planning therapy.

Objective To analyze the possible effects of mitochondrial DNA mutation on noise-induced hearing loss.Methods 265 young workers with noise exposure were examined.The average pure tone thresholds at 500,1 000,and 2 000 Hz for 25 voluntary persons were noise-induced hearing loss by nature and selected as the study group.27 voluntary persons with no speech frequency hearing loss were chosen as the control group.DNA was extracted from peripheral blood leukocytes.Polymerase chain reaction(PCR) was used to detect the mtDNA4977 deletion in both groups.Results The detection rate of mtDNA4977 deletion in the study group was 20%(5/25) and it was 0(0/27) for the control group.Conclusion MtDNA4977 deletion in the study group was significantly higher than that of the control group,indicating that mtDNA4977 deletion may play an important role in the development of noise-induced hearing loss.

Objective To investigate the diagnostic value of ultrasound elastography hardness score and area ratio to identify benign and malignant lesions.Methods 90 patients with breast lumps were selected and divided into the malignant and non -malignant group according to surgical pathology diagnosis,they were 40 cases (a total of 45 tumors)and 50 cases (a total of 57 tumors),two groups of patients were detected in ultrasound elastography,anal-ysis of imaging hardness ratings and area ratio and other information,to investigate the clinical diagnostic value. Results The ultrasound elastography of 50 cases with benign tumor were 0 -2 point,7 cases were 3 -4 point, 7 cases with malignant tumors were 0 -2 point,38 cases were 3 -4 point,the difference between the two groups were statistically significant (χ2 =28.55,P <0.05);The average area ratio of benign tumor was (1.01 ±0.27),malignant tumors was (2.28 ±1.68),the difference between the two groups was statistically significant(t =9.22,P =0.001);the sensitivity,specificity,accuracy of elastography hardness rating in diagnosis of malignant breast tumors were 87.72%,84.44%,86.27%,the area ratio method were 85.96%,86.67%,86.27%,joint inspection of the two groups were 96.49%,95.55%,96.08%,joint inspection had obvious advantages,the difference was statistically sig-nificant(χ2 =16.24,13.58,P <0.05).Conclusion Ultrasound elastography hardness rating combined area ratio has a higher accuracy rate for differentiating benign and malignant breast tumors.

This study aimed at comparing the United States, Britain, Australia, the Netherlands and China’s national performance evaluation, and sum up the experience to provide a theoretical basis for the China’s development of hospital performance evaluation system. The study found that China needs to consider the patient's perspective, to establish a fixed third-party performance evaluation agencies, establish an effective incentive mechanism and feedback mechanism and combine a variety of assessment methods in the development of hospital performance evaluation index system.

Objective To explore the effect of different factors on strengthening the tissue culture seedlings and roots of Dendranthema morifolium ( Ramat) Tzvel. cv. Gongju, so as to provide experimental basis for establishing an in-vitro rapid propagation system of Dendranthema morifolium. Methods The experiment took Dendranthema morifolium ( Ramat) Tzvel. cv. Gongju tissue culture seedling as the studying object. By using tissue culture technology, different additives were added into the MS medium. Results In the seedling-strengthening culture medium, 150 mL/L of macro-element was beneficial to the growth of tissue culture seedlings of Dendranthema morifolium ( Ramat) Tzvel. cv. Gongju. The culture medium with 100 g/L of coconut milk added could obviously promote the growth of Dendranthema morifolium ( Ramat) Tzvel. cv. Gongju tissue culture seedlings. And activated carbon added to the rooting culture medium could induce the formation of tissue culture root system of Dendranthema morifolium ( Ramat) Tzvel. cv. The addition of α-naphthaleneacetic acid ( NAA) and indolebutyric acid ( IBA) at certain concentrations could promote the amount and the growth of the roots. Conclusion The tissue culture seedlings of Dendranthema morifolium ( Ramat) Tzvel. cv. Gongju are boosted by increasing the amount of macro-element, reducing the concentration of agar in the culture medium and adding coconut milk and growth hormones to the culture medium. The optimal rooting culture medium is a compound of 1/2MS, NAA 0.05 mg/L and IBA 0.05 mg/L.