Female rats were divided into three groups: 1) LI group, receiving low iodine diet and distilled water; 2) NI group being fed with the same food as LI group and distilled water supplemented with potassium iodide; and 3) N group,taking usual diet and tap water. T4 5'-deiodinase, T, and T4 content in whole brain homogenate and serum T, and T4 level of the young animals delivered by the above-mentioned rats were analyzed. Deiodinase activity was expressed in terms'of T3→T4 conversion rate (CR). The results showed that the average CR in rat brain homogenates of LI group was significantly higher than those of the other two groups, it is 2.73 times of that in N group. There were no significant differences among the three groups in serum T3 concentration and T3 content of brain homogenate. But serum T4 concentration of LI group was only 10.9% of that in N group and brain homogenate T4 level was also lower, being only 38.5% of the latter. It suggested that T4 5'-deiodinase in the immature rat brain is more effective than in adult one on modulating thyroid hormone function in brain. Thus, the increased activity of brain T4 5'-deiodinase may compensate for the decreased T4 pool, maintaining a normal brain T3 content in congenital iodine-deficient rat.

The authors have applied the Omaha system in six different researches conducted in Hong Kong and mainland China in the last ten years. These researches have been conducted using different client groups,specialty nurses,and in the hospital,clinic and community settings. The Omaha system was found to be instrumental in serving as a conceptual framework for study,designing the intervention protocol,identifying clients' health problems,defining nursing interventions and evaluating research outcomes. The Omaha system is a valuable tool for research and practice and is worth exploring further for its application in mainland China,particularly in the areas of community nursing and transitional care.

Objective:To study the phenotypes of T cell in patients with Hashimoto thyrioditis (HT) and to analyze its relationship with the impairment of follicles, so as to provide evidence for clinical diagnosis and treatment. Methods: Totally 136 HT specimens were obtained by fine needle aspiration biopsy (FNAB) and were divided morphologically(L, P, O type). The serum parameters (TPOAb, TGAb, TSH) were tested, and 20 cases with nontoxic goiter were taken as control. Immuno-histochemical assay was performed with 60 specimens and FCM (Flow cytometry ) was used to study 23 specimens. The T cell infiltration and the distribution of CD3+ . CD4+ . and CD8+ T cells were compared between all types of HT tissues. The correlationship between the atrophic degree of follicle with T lymphocytes infiltration and phenotype was analyzed. Results: It was found that L type (n = 58) and P type (n = 28) had more serious follicle atrophy than that of O type (n = 20) (P

Aim: To establish a method for the determination of pantoprazole sodium and its related substances. Methods: A column packed with octadecylsiance bonded silica gel (250 mm × 4. 6 mm, 5 pjn) was used. The 0. 01 mol/L monopotassium phosphate solution( adjusted with phosphoric acid to pH 7. 0) -acetonitrile were adopted as the mobile phase, a gradient elution was programmed as follows: 0→30 min(90:10-60:40), 30→45 min(60: 40→15: 85); the detection wavelength was 289 nm; the column temperature was 40 ℃; the flow rate was 1. 0 mL/min. Results: Pantoprazole sodium, the intermediates and its related substances could be well separated. A good linear relationship was obtained over the range of 6. 96-48. 72 μg/mL( r =0. 999 9). The limit detection and quantisation of pantoprazole sodium were 8.51 ng and 17.0 ng, respectively. Conclusion: This method can be applied to control the related substances of pantoprazole sodium and determine pantoprazole sodi-um.

[Abstract ] Objective To understand the infection status of hepatitis B among population of health examination in Chongqing ,and to analyze the modes of hepatitis B outcomes .Methods The 30170 cases of serological results of hepatitis B (HB‐sAg① ,HBsAb② ,HBeAg③ ,HBeAb④ and HBcAb⑤ ) among the individuals of health examination in our hospital from June to December 2013 were performed the retrospective analysis .Results The positive rate of HBsAg among the population of health ex‐amination aged 0-96 years old in Chongqing was 8 .02% (2 419/30 170) ,and the differences in the positive rates among different age groups and between different genders had statistical significance (χ2 =188 .045 ,67 .813 ,P<0 .01) .The infection rate of HBV was 28 .64% (8 641/30 170) ,and the difference in the infection rates among different age groups and between different genders also had statistical significance(χ2 =202 .347 ,36 .171 ,P<0 .01) .30 170 cases of hepatitis B detection results of 5 indexes were conclu‐ded into 14 kinds of modes .As the two common modes of healthy population ,all negative and single HBsAb positive ,their percenta‐ges were 25 .76% (7 772/30 170)and 45 .60% (13 757/30 170)respectively .As the common modes of HBV infection ,“① ④ ⑤”posi‐tive ,“① ③ ⑤” positive and“① ⑤” positive ,their percentages were 6 .18% (1 863/30 170) ,0 .59% (177/30 170) and 1 .21% (366/30 170) respectively .Conclusion The positive rate of HBsAg and the infection rate of HBV among population of health examina‐tion in Chongqing are close to or lower than the national average levels .Analyzing the different combination modes can reflect the different situation of HBV infection and has the guidance significance for the diagnosis and prognosis of the disease .

Objective To explore the impact of enteral nutrition (EN)support on patients with advanced cancer.Methods 83 cases with advanced cancer were selected,the patients were randomly divided into two groups, 42 cases in the observation group,41 cases in the control group.The observation group was given EN support com-bined with parenteral nutrition (PN)support,while the control group was only given the PN support.Immune function CD +4 ,CD +4 /CD +8 ,IgA,IgG,nutrition indicators [serum albumin (ALB),transferrin (TF)and 24h urea nitrogen (24hUP)]before and 10d after surgery as well as complications were compared.Results The differences of CD +4 , CD +4 /CD +8 ,IgA,IgG,ALB,TF and 24h UP between the two groups before surgery were not statistically significant (P >0.05);10d after surgery,CD +4 ,CD +4 /CD +8 ,IgA,IgG levels in the control group were (52.4 ±4.4)%,(1.8 ± 0.3)%,(2.7 ±0.3)g/L and (11.4 ±2.1)g/L,which in the observation group were (55.9 ±5.6)%,(2.1 ± 0.5)%,(2.9 ±0.5)g/L and (12.9 ±2.3)g/L,CD +4 /CD +8 ,IgA,IgG were significantly higher in the observation group(t =2.408,2.521,2.332,2.359,all P <0.05);10d after surgery,ALB,TF and 24h UP in observation group were (36.6 ±2.3)g/L,(2.9 ±0.2)g/L,and (0.7 ±0.3)g/L,which were (32.4 ±1.5)g/L,(2.2 ±0.1)g/L,and (0.4 ±0.1)g/L in the control group,the differences were statistically significant (t =7.493,15.34,4.648,P <0.05).The incidence rate of complications in the observation group was 9.5% (4 /42),which in the control group was 26.8%(11 /41),the difference was statistically significant (χ2 =4.196,P <0.05).Conclusion EN support for patients with advanced cancer is benefit for immune function improvment and nutrition improvement,and can reduce the risk of complications.

Objective To study the effect of Zhuang'er Granules(ZG) on cellular immune function of mice.Methods The immunodepression mice models were induced by injection of hydrocortisone.Then the lymphocyte transformation rate was examined by morphological test and phagocyte phagocytosis by in vitro method after 7-day intragastric administration of the drugs.Results Compared with the negative control group,ZG could significantly improve the lymphocyte transformation rate and phagocyte phagocytosis in the normal mice and immunodepression mice(P

Objective To summarize the X-ray and CT manifestations of dialysis- related osteoarthropathy. Methods The study consisted of 20 cases with long-term dialysis patients. The X-ray and CT scanning of their shoulder, knee, hip, wrist and cervical vertebrae were performed respectively. The X-ray and CT manifestations of dialysis- related osteoarthropathy were observed. Results The roentgenographic and CT features included:universal osteoporosis(n=20), bone resorption(n=17), bone sclerosis(n=15), calcification(n=19) . Conclusion X-ray and CT are of significance in diagnosing bone resorption and calcification.

Aim To evaluate whether the combination of polypeptide AP25 and docetaxel is more efficient in treating experimental breast cancer,than either reagent used alone,and to offer suggestions for clinical use. Methods An experimental breast carcinoma model was set up to investigate the anti-tumor effects of AP25 and docetaxel combination.The Q value was caluculat-ed by Guinness rules and the anti-tumor effects of the combination of polypeptide AP25 and docetaxel were e-valuated.Results The treatment by the combination of polypeptide AP25 and docetaxel showed a better tumor inhibition rate.The combination of AP25 20 mg ·kg -1 and docetaxel 10 mg·kg -1 significantly inhibi-ted the tumor growth with 0.85 1.15,showing a synergistic effect.Conclusions The combination of AP25 and docetaxel can significantly in-hibit the tumor growth with a synergistic effect and de-crease the dose of chemotherapy.