Female rats were divided into three groups: 1) LI group, receiving low iodine diet and distilled water; 2) NI group being fed with the same food as LI group and distilled water supplemented with potassium iodide; and 3) N group,taking usual diet and tap water. T4 5'-deiodinase, T, and T4 content in whole brain homogenate and serum T, and T4 level of the young animals delivered by the above-mentioned rats were analyzed. Deiodinase activity was expressed in terms'of T3→T4 conversion rate (CR). The results showed that the average CR in rat brain homogenates of LI group was significantly higher than those of the other two groups, it is 2.73 times of that in N group. There were no significant differences among the three groups in serum T3 concentration and T3 content of brain homogenate. But serum T4 concentration of LI group was only 10.9% of that in N group and brain homogenate T4 level was also lower, being only 38.5% of the latter. It suggested that T4 5'-deiodinase in the immature rat brain is more effective than in adult one on modulating thyroid hormone function in brain. Thus, the increased activity of brain T4 5'-deiodinase may compensate for the decreased T4 pool, maintaining a normal brain T3 content in congenital iodine-deficient rat.

The authors have applied the Omaha system in six different researches conducted in Hong Kong and mainland China in the last ten years. These researches have been conducted using different client groups,specialty nurses,and in the hospital,clinic and community settings. The Omaha system was found to be instrumental in serving as a conceptual framework for study,designing the intervention protocol,identifying clients' health problems,defining nursing interventions and evaluating research outcomes. The Omaha system is a valuable tool for research and practice and is worth exploring further for its application in mainland China,particularly in the areas of community nursing and transitional care.

Aim: To establish a method for the determination of pantoprazole sodium and its related substances. Methods: A column packed with octadecylsiance bonded silica gel (250 mm × 4. 6 mm, 5 pjn) was used. The 0. 01 mol/L monopotassium phosphate solution( adjusted with phosphoric acid to pH 7. 0) -acetonitrile were adopted as the mobile phase, a gradient elution was programmed as follows: 0→30 min(90:10-60:40), 30→45 min(60: 40→15: 85); the detection wavelength was 289 nm; the column temperature was 40 ℃; the flow rate was 1. 0 mL/min. Results: Pantoprazole sodium, the intermediates and its related substances could be well separated. A good linear relationship was obtained over the range of 6. 96-48. 72 μg/mL( r =0. 999 9). The limit detection and quantisation of pantoprazole sodium were 8.51 ng and 17.0 ng, respectively. Conclusion: This method can be applied to control the related substances of pantoprazole sodium and determine pantoprazole sodi-um.

[Abstract ] Objective To understand the infection status of hepatitis B among population of health examination in Chongqing ,and to analyze the modes of hepatitis B outcomes .Methods The 30170 cases of serological results of hepatitis B (HB‐sAg① ,HBsAb② ,HBeAg③ ,HBeAb④ and HBcAb⑤ ) among the individuals of health examination in our hospital from June to December 2013 were performed the retrospective analysis .Results The positive rate of HBsAg among the population of health ex‐amination aged 0-96 years old in Chongqing was 8 .02% (2 419/30 170) ,and the differences in the positive rates among different age groups and between different genders had statistical significance (χ2 =188 .045 ,67 .813 ,P<0 .01) .The infection rate of HBV was 28 .64% (8 641/30 170) ,and the difference in the infection rates among different age groups and between different genders also had statistical significance(χ2 =202 .347 ,36 .171 ,P<0 .01) .30 170 cases of hepatitis B detection results of 5 indexes were conclu‐ded into 14 kinds of modes .As the two common modes of healthy population ,all negative and single HBsAb positive ,their percenta‐ges were 25 .76% (7 772/30 170)and 45 .60% (13 757/30 170)respectively .As the common modes of HBV infection ,“① ④ ⑤”posi‐tive ,“① ③ ⑤” positive and“① ⑤” positive ,their percentages were 6 .18% (1 863/30 170) ,0 .59% (177/30 170) and 1 .21% (366/30 170) respectively .Conclusion The positive rate of HBsAg and the infection rate of HBV among population of health examina‐tion in Chongqing are close to or lower than the national average levels .Analyzing the different combination modes can reflect the different situation of HBV infection and has the guidance significance for the diagnosis and prognosis of the disease .

Objective:To study the phenotypes of T cell in patients with Hashimoto thyrioditis (HT) and to analyze its relationship with the impairment of follicles, so as to provide evidence for clinical diagnosis and treatment. Methods: Totally 136 HT specimens were obtained by fine needle aspiration biopsy (FNAB) and were divided morphologically(L, P, O type). The serum parameters (TPOAb, TGAb, TSH) were tested, and 20 cases with nontoxic goiter were taken as control. Immuno-histochemical assay was performed with 60 specimens and FCM (Flow cytometry ) was used to study 23 specimens. The T cell infiltration and the distribution of CD3+ . CD4+ . and CD8+ T cells were compared between all types of HT tissues. The correlationship between the atrophic degree of follicle with T lymphocytes infiltration and phenotype was analyzed. Results: It was found that L type (n = 58) and P type (n = 28) had more serious follicle atrophy than that of O type (n = 20) (P

This paper is to report our study in which the differences between prosthetic restoration and surgical reconstruction using traditional clasp retention technology were analyzed based on three-dimensional finite element methods in our laboratory. Firstly, the maxillary unilateral defect model was developed using medical image processing software MIMICS. Secondly, the prosthesis was generated by mirroring technology. The clasp was designed according to the methods raised by Aramany. Then, the stress distribution of maxilla was calculated by simulating occlusion. According to the results, after osseointegration of surgical reconstruction, stresses of unaffected abutments were reduced significantly, and less stress of junction occurred near zygoma of affected side, which were all less than stresses of prosthesis restoration. Thus, removing the clasp of surgical reconstruction increased the stresses of unaffected abutments. The stress trends of maxillary components were different between prosthetic restoration and surgical reconstruction. Surgical reconstruction is better than prosthesis restoration in protection of the abutments. Clasp can alleviate the occlusal burden of maxilla. Varieties of retentive technologies can be considered in prosthesis restoration. The surgical reconstruction is more conducive to rehabilitate unilateral maxilla biomechanically in clinic.
Biomechanical Phenomena ; Finite Element Analysis ; Humans ; Image Processing, Computer-Assisted ; Mandibular Reconstruction ; methods ; Maxilla ; surgery ; Maxillofacial Prosthesis ; Osseointegration ; Zygoma

Objective To study the effect of Zhuang'er Granules(ZG) on cellular immune function of mice.Methods The immunodepression mice models were induced by injection of hydrocortisone.Then the lymphocyte transformation rate was examined by morphological test and phagocyte phagocytosis by in vitro method after 7-day intragastric administration of the drugs.Results Compared with the negative control group,ZG could significantly improve the lymphocyte transformation rate and phagocyte phagocytosis in the normal mice and immunodepression mice(P

Lobaplatin is the third generation platinum anticancer drug,and has been listed for the clinical treatment of advanced breast cancer,small cell lung cancer and chronic myeloid leukemia.Studies have shown that lobaplatin as the latest third generation platinum anticancer drug,has good water solubility,broadspectrum anti-tumor,strong anti-tumor activity,no cross-resistance with other platinum drugs,low toxicity and many other features.Lobaplatin has shown a clear advantage.

Objective:To investigate the relationship between the gene polymorphism cluster of differentiation 14 (CD14)-159C/T (rs2569190),and interleukin-8 (IL-8)-251A/ T (rs4073)and the susceptibility of necrotizing enterocolitis (NEC),to clarify the influencing factors of susceptibility of NEC and to provide genetics theory basis for the research on the pathogenesis of NEC. Methods:Total 28 newborns with NEC and 41 newborns without NEC were selected.The amplification of peripheral blood DNA was conducted by PCR.The genotypic and allelic frequencies of CD14-159C/T and IL-8-251A/T of the patients were detected by Sanger DNA sequencing method. The relationship between them and the susceptibility of NEC was studied.Results:The distribution of genotypic frequencies of CD14-159C/T and IL-8-251A/T was consistent with Hardy-Weinberg equilibrium (P >0.05).There were no significant differences of the allelic and genotypic frequencies of CD14-159C/T,or genotypic frequencies of IL-8-251A/T between two groups (P >0.05).While in NEC group,the T allelic frequency of IL-8-251A/T site was higher than that in control group (χ2 = 4.184, P = 0.041, OR = 2.14, 95% CI: 1.03 - 4.46 ). Conclusion:The polymorphism of CD14-159C/T is irrelevant to the pathogeny of NEC,but T allelic frequency of IL-8-251A/T site might be related to the susceptibility of NEC.So T allele in IL-8-251A/T may be one of the danger factors of NEC.