Objective To study the distribution and drug resistance of clinical Providencia stuartii isolates and provide the basis data for clinical therapy.Methods A data analysis for the specimen source,the distribution of departments with infection and the antibiotic susceptibility results to 76 isolates was conducted in retrospectively. Results Among 76 strains were mainly from sputum (71.1%),wound secretions (10.5%) and blood (6.6%);the proportion of these strains from ICU was 40.8%,from respiratory department was 9.2%and from general surgery was 7.9%;The bacteria's drug resistance was more serious,multi drug resistant (MDR) strains accounted for 65.8%,pan drug resistant ( PDR) strains accounted for 26.3%,the mortality of patients with PDR strains infection was 45.0%,which was higher than 5.8%of no PDR strains infection.A low resistance rate of antibiotics were imipenem,fluoro-quinolones andamikacin,et al,and the rate of imipenem resistant was lowest(31.5%).Conclusion The multidrug resistance phenomenon of Providencia stuartii is serious, the first choice for the treatment is imipenem, the second choice was fluoroquinolones or amino glycopeptides antibiotics,the hospital infection caused by the bacterium infection in monitoring should be strengthened.

Urinary microquantitative albumin (UALb), immunoglobulin G (UIgG) ?2-microglo-bulin (U?2m), and Tamm-Horsfall protein (THP) were measured in 36 chronic B hepatitis (CHB) and 23 liver cirrhosis (LC) patients with radioimmunoassay. 109 healthy normal subjects served as controls. The results showed that the excretion of UALb was significantly increased in 17 patients. Out of 17 patients,the excretion of UIgG in 5 and U?2m in 16 were increased and the excretion of THP in 9 patients were decreased. Compared with normal controls there was a significant difference (P

OBJECTIVETo investigate the effect of local administration of recombinant adenovirus of human adiponectin (hAPN) Ad-hAPN-EGFP on tibial defect repair of SD rats.

METHODSTibial defect (2 mm) models of 36 SD rats (72 sides) were randomly divided into three groups (A, B, and C; n=24). The three groups were injected with Ad-hAPN-EGFP, Ad-EGFP, and normal saline, respectively, during operation and the day after operation. A week after the operation, the expression of hAPN and osteogenesis-related factors were detected by real-time polymerase chain reaction. Three weeks after operation, the tibias were examined by micro-computed tomography, hematoxylin-eosin staining, and Masson staining to evaluate the restoration of bone defects.

RESULTS1) The hAPN expression was detected in group A but not in groups B and C. Osteogenesis-related factors expression of group A was significantly higher than that of the other groups (P<0.05). 2) Osteogenesis (including bone mineral density, relative bone volume, trabecular number and trabecular thickness) in group A were more evident than those in groups B and C (P<0.05). No significant differences were found between groups B and C (P>0.05).

CONCLUSIONLocal administration of recombinant adenovirus Ad-hAPN-EGFP may be an effective strategy to improve the restoration of bone defects in vivo.

Adenoviridae ; Adiponectin ; Animals ; Bone Density ; Bone and Bones ; Humans ; Osteogenesis ; Rats ; Rats, Sprague-Dawley ; Real-Time Polymerase Chain Reaction ; X-Ray Microtomography

Abstract In recent years, the legal status of school based sexuality education in China is becoming more and more clearly defined, so that the content is becoming more and more comprehensive. It will be improved and optimized under the guidance and support of newly launched policies. At the initial stage of development, school based sexuality education will face certain challenges, including the need of raising national awareness of sexuality education and establishing correct sexual values, researches and development of supporting teaching resources on school based sexuality education, including curriculum of teaching material of sexuality education, teacher training, and teaching effectiveness assessment. This paper will analyze the challenges of school based sexuality education in China, in order to explore the most suitable ways of implementing school based sexuality education in China.

Objective: To understand the current situation of school based sexuality education, attitudes and evaluations of teachers and students toward sexuality education, so as to provide evidence for promoting the development of school based sexuality education. Methods: From September to October 2021, 2 140 valid data from students in basic education and 2 146 from kindergarten and primary and secondary school teachers were collected by online questionnaire. The data were described and statistically analyzed, and the influencing factors of teachers willingness to teach sexuality education courses were explored by logistic regression analysis. Results: About 52.06% of the students had not received sexuality education in school, and 47.53% of the teachers did not carry out sexuality education in the school. Sexuality education in schools mainly focuses on reproductive health and physical development. About 48.93% of the teachers thought that the implementation of sexuality education in schools was not good, and the proportion of high school students who thought sexuality education in schools were "very bad" (32.87%) or "not very good" ( 21.23 %) was the highest. The teachers who undertook sexuality education were mainly mental health teachers (55.51% reported by teachers, 43.96% reported by students). About 71.02% of teachers believe that the best way to carry out sexuality education classes was to set up sexuality education curriculum. Students and teachers reported the need for sexuality education, and more than 50% of primary school students and high school students thought that sexuality education were "very needed" and "somewhat needed". About 51.77 % of teachers considered sexuality education "very urgent". All increased recognition of sexuality education, confidence in answering sex questions, external support for sexuality education, and acceptance of sexuality education training increased the likelihood of teachers teaching sexuality education ( OR =1.02, 1.95, 1.03, 3.53, P <0.05). Conclusion The current situation of school based sexuality education is not optimistic, the gap between sexuality education and students needs is wide, and the abilities of sexuality education teachers are insufficient.

Objective: To investigate the experience, attitude and practice of sexuality education for teachers, so as to provide evidence for promoting sexuality education training for teachers and teaching ability improvement. Methods: From September to October 2021, 2 146 valid data of kindergarten and primary and secondary school teachers across the country were collected. Associateds factors of teachers sexuality education behavior were explored by Logistic regression analysis. Results: Family and school based sexuality education experiences were insufficient among teachers (22.51%, 46.55%), and the proportions of female teachers who had received sexuality education (20.44%, 41.67%) were significantly higher than that of male teachers (2.05%, 4.90%). Currently, 47.53 % of the kindergartens and primary and secondary schools from participating teachers didn t carry out sex education, which mainly focused in the field of reproductive health and physical development. Kindergartens and primary and secondary schools often integrated sex education into other disciplines (64.48%, 47.83%). Teachers acceptance of family and school based sexuality education, students willingness to communicate on sexual topics with teachers, teachers confidence in answering sexual questions were positively correlated with teachers teaching activities of sexuality education ( OR=1.59, 1.37, 1.67, 1.67, P < 0.01 ). Conclusions Teachers teaching ability of sexuality education is insufficient, and school based sexuality education couldn t meet students developmental needs. Providing sexuality education training to teachers and enhancing their confidence in answering sexual questions are helpful to facilitate sexuality education teaching activities.

Abstract In recent years, school-based sexuality education was attached with great importance by more and more countries and educators. However, in China, the implementation and promotion of school-based sexuality education is still facing a number of challenges, including course/class hours, teaching materials, training of teachers as well as the collaboration among schools, families and communities, etc. This paper aims to learn from advanced experience of sexuality education in Macau, in order to promote the development of school-based sexuality education in Mainland China.

Abstract School-based sexuality education continues to face problems and challenges, such as schools are unable to carry out their main role in regards to the provision of sexuality education, a shortage of teachers specialized in teaching this subject and the absence of a separate and dedicated curriculum that focuses on comprehensive sexuality education teaching content. In order to promote school-based sexuality education, it is necessary to promote a comprehensive curriculum that enhances content relevance, while continuously improving and optimizing national policies on sexuality education by focusing on more accurate policy making, refining implementation methods, and strengthening the synergistic effect of school-based sexuality education policies.

Objective: To study the effect of p-phenylenediamine (PPD) on liver and kidney function in occupational exposed workers. Methods: Workers in a hair dye production enterprise which used p-phenylenediamine as a raw material for production were selected as the main research population. Then we conducted a questionnaire survey on the basic conditions of workers and conducted occupational health checkups on general health status, liver and kidney function. Occupational health examination assessment results were tested in Taizhou Cancer Hospital. All data was built using EpiData 3.1 software, and statistical analysis was performed using software SPSS 20.0. Results: The liver function indicators including direct bilirubin, prealbumin, total protein, and white protein, globulin, aspartate aminotransferase, glutamyl transpeptidase, and total bilirubin in the workers exposed to high concentration of PPD were at high normal values, and these indicators were significantly different from low PPD concentration group (P<0.05) . The serum creatinine and serum uric acid in the renal function index were significantly higher in workers exposed to PPD than in workers exposed to low concentrations and in the control group (P<0.05) . Conclusion Occupational exposed to PPD may have a hazard to the workers’ liver and kidney function. Long-term occupational exposure to PPD may lead to increased cumulative exposure of workers, which may cause potential chronic liver and kidney damage in occupationally exposed populations.

Objective: To understand the current situation of sexual knowledge, attitudes and behaviors of young children in kindergarten, based on the framework of comprehensive sexuality education. Methods: Totally 354 children between the ages of 3-6 from two kindergartens in Changping District, Beijing, participated in one-to-one interviews. There were 164 girls and 190 boys. Results: In the dimension of physical development, no child knew the scientific name of reproductive organs. In the dimension of social emotion, 44.07% of children could express love to their friends, and 40.68% of children could express their refusal when they were unwilling. In the dimension of sexual health, well-being and sexual rights，81.92% of children knew that they couldn t touch other people s genitals, and 54.80% of children could at least name one way to protect themselves when facing sexual abuse. In the dimension of gender equality, 33.62% and 28.53%of children had gender stereotypes in toy selection and career choice, 66.38% of children thought that boys are as smart as girls. In the dimension of pregnancy and birth，78.53% of children knew where babies come from and 26.27% of children think parenthood doesn t directly lead to happiness. Sexual knowledge, attitudes and behaviors of children had statistically significant difference in different grades（P<0.05). Conclusion Children already have good understanding about pregnancy, childbirth and prevention of sexual abuse. In the future, sexuality education in school and family need to promote physical cognition and gender equality education.