Objective To assess the effectiveness of Exenatide on non‐alcoholic fatty liver disease(NAFLD) with diabetes mellitus by evaluating fatty liver index(FLI) variation .Methods One hundred and two NAFLD patients with type 2 diabetes melli‐tus were enrolled and divided into two groups .One group were treated with Exenatide and another with Oral antidiabetic agents . The data of BMI ,blood fat ,HbA1c ,ALT ,AST ,GGT ,FLI and so on were collected at enrollment and the end point of 24 weeks treatment .The effectiveness of Exenatide were analysed by Kolmogorov‐Smirnov and Pearson correlation coefficient (r) analysis . Results The indicators of metabolic disorder ,the parameters of liver biochemistry and fatty liver disease (FLI) were significantly improved (P<0 .05) after 24 weeks treatment by Exenatide .While only BMI showed significant decrease after 24 weeks treatment with Oral antidiabetic agents(P<0 .05) .The rest of the parameters in oral antidiabetic agants group showed no significant differ‐ence (P>0 .05) .Conclusion Exenatide has a positive effect on non‐alcoholic fatty liver disease treatment who with type 2 diabetes mellitus ,especially for liver fat accumulation .

Inflammatory bowel disease (IBD) is a group of chronic, non-specific inflammatory intestinal diseases.Patients with IBD predispose to the development of colitis-associated colon cancer (CACC).Homocysteine is an important intermediate metabolite in methionine cycle, increased level of homocysteine is closely correlated with the development and progression of IBD and CACC.This paper reviewed the advances in study on correlation of homocysteine with IBD and CACC.

Objective To explore the difference of DISC1 gene polymorphism between schizophrenia patients and normal subjects,as well as the association of gene polymorphism with mRNA expression.methods 40 schizophrenia patients and 40 normal subjects were recruited randomly from southern region of Fujian,China.DISC1 mRNA level was assessed by RT-PCR and the genotype was evaluated by sequencing with the amplified PCR products from peripheral blood DNA.Result srs6675281 locus only found CC type,other types were not found.According to the genotyping Result ,the rs821616 locus has AA,AT and TT three types,but the genotype and allele frequency between the two groups were not significantly different (Genotype:x2=0.923,P=0.63;Allele:A>T,x2=0.656,P=0.418).As far as rs11122319 locus,AA,AG and GG three types were found in this study,there was no significant difference between patients and normal controls (Genotype:x2=3.922,P=0.141;Allele:A>G,x2=0.184,P=0.668).Subjects were divided into AA,AG,GG three types based on rs1417584 locus genotyping,however the genotypes and alleles of this locus between the two groups were significant difference (Genotype:x2=6.631,P=0.042;Allele:A>G,x2=4.592,P=0.032),and the DISC1 mRNA expression that corresponding to the genotype AG in patients and normal subjects was significantly different (t=3.916,P=0.004).Conclusion Based on these findings,rs1417584 locus may be implicated the expression and regulation of DISC1 gene,may be a pathogenic factor of schizophrenia and the genotype AG may be corresponding to a higher risk.

Objective To explore the changed rules and the diagnositic methods of the cardisc functions after chest impact trauma(CIT).Methods The medals of moderate to severity CIT were established using BIM-Ⅱ Bio-impactor in 20 rabbits.The cardiac functions were examined with cardiac catheterization,single photon-emission computed tomography(SPECT)and the Doppler echocardiography at pre end post 1h,4h,8h and 24h after CIT.Results The cardiac functions were changed significantly after CIT.The expressions of the right ventricular dysfunctions mainly were systolic dysfuction while the left ventricular dysfunctions mainly were diastolic dysfunction after CIT.Conclustion All the cardiac catheterization、SPECT and the Doppler echocardiography are beneficial to the diagnosis of cardiac dysfunction afte CIT.The SPEGT is more exactitude and the Doppler echocardiography is more cheaper compared with non-invasive approaches.

The history of automated blood cell analysis and current situation regarding the peripheral blood smear review following automated complete blood count(CBC) and leukocyte differential count(LDC) was introduced. Principles and procedure of determining criteria for blood smear review in clinical laboratory was elucidated in combination with 41 consensus rules for the review of automated CBC and LDC proposed by the International Consensus Group for blood smear review.

Objective To explore the changes and the significance of left ventricular functions after blunt chest trauma(BCT)in rabbits.Methods 36 rabbit models of BCT with BIM-Ⅱ Bio-impactor in were used to observe the changes of left ventricular functions and free calcium,free calmodulin and total calmodulin were detected at pre-injury,the 2nd,4th,8th,12th and 24th hour after injury.Results The cardiac function were impaired.The systolic functions of left ventricle were impaired and recovered during 4-12 hour after BCT.The diastolic functions of left ventricle were impaired but not recovered 24h after BCT.The free calcium and total calmodulin in myocardial cells were increased from the 4th hour,reached,peak at the 8th hour post-BCT(P＜0.01),and then decreased,but were still higher at the 24th hour post-BCT than that of pre-BCT(P＜0.05).The free calmodulin in myocardial cells was low and reached peak at the 8th hour after BCT(P＜0.01).There is remarkable positive correlation between free calcium in myocardial cells and LVEDP and dp/dtmax with cardiac function(P＜0.05,P＜0.01).Conclusion The cardiac function is obviously changed after BCT,especially the diastolic functions of left ventricle.The hish concentration of free calcium in myocardial ceils may be one of the reasons of cardiac dysfunction after BCT.

Adult ; Antineoplastic Agents ; adverse effects ; Chromosome Aberrations ; chemically induced ; DNA Damage ; Female ; Humans ; Micronucleus Tests ; Nursing Staff, Hospital ; Occupational Exposure

Objective; To investigate the associations between the levels of human plasma anti-gliadin antibodies and occurrence of schizophrenia in the patients with different ages in Chinese Han population, and to clarify the effects of human plasma anti-gliadin antibodies in the occurrence of schizophrenia. Methods; The plasma samples were collected from 313 schizophrenia patients (schizophrenia group) and 408 healthy controls (healthy control group) in Chinese Han population. The levels of human anti-gliadin IgA and IgG antibodies of the subjects in various groups were detected by enzyme-linked immnosorbent assay (ELISA) method. The levels of human plasma anti-gliadin IgA and IgG antibodies in the subjects in schizophrenia group and healthy control group were analyzed by Pearson's correlation analysis and compared by Mann-Whitney U test; receiver operating characteristic (ROC) curve analysis was performed. Results; The level of plasma anti-gliadin IgA antibody showed a significantly positive correlation with the age and age ranges of the subjects (r= 0. 177, P<0. 01; r= 0. 171, P<0. 01); the level of plasma anti-gliadin IgG antibody showed significantly negative correlation with the age and age ranges of the subjects (r=- 0.104, P<0. 01; r= - 0.127, P<0. 01). Compared with healthy control group, the levels of plasma antigliadin IgA antibodies of the patients aged 20-30 years old and 50-60 years old in schizophrenia group were increased (Z= - 3. 746, P<0. 01; Z= 2. 186, P<0. 05). Compared with healthy control group, the level of plasma anti-gliadin IgG antibody of the patients aged 20-30 years old in schizophrenia group was increased, but the difference was not statistically significant (P>0. 05). The ROC curve analysis results showed that the area under ROC curve (AUC) of anti-gliadin IgA and IgG antibodies were 0.573 (SE = 0. 022, 95% Cl: 0.53-0.62) and 0. 520 (SE=0. 022, 95%CI; 0. 48-0. 56). The sensitivities of anti-gliadin IgA and IgG antibodies were 13. 7% and 12. 8% when the specificity was 92. 2%. Conclusion; The increasing of plasma anti-gliadin antibodies level is associated with the occurrence of schizophrenia in the patients with different age ranges in Chinese Han population, and the human plasma anti-gliadin antibodies maybe play an important role in the young schizophrenia patients.

Aim To investigate the effect of hesperidin on human lung cancer cell A549 and the possible mechanism.Methods The cell apoptosis and necrosis of A549 treated with hesperidin were measured by the Hoechst 33342/PI fluorescent dye based on microfluidic chip technology.Cell cycle and apoptosis rate were evaluated by flow cytometry(FCM).The expressions of the related genes were detected through the real-time fluorescent quantitative PCR technology(RT-PCR) including VEGF, PI3K and PTEN.The protein expressions of Bcl-2, Cyclin B1, PI3K, Akt and PTEN were detected by Western blot after hesperidin intervention.Results The proliferation of A549 cells was significantly inhibited by hesperidin in a dose-dependent manner.FCM results showed that hesperidin could not only influence the G0/G1 phase and S phase, but also promote the apoptosis of lung cancer cells.Meanwhile, the apoptosis and necrosis rate was increased from(6.7±0.6)% to(27.9±1.1)% compared with that of control group(P<0.05).From the level of molecular, the gene expressions of VEGF and PI3K were decreased, while the PTEN was increased after hesperidin stimulation.Western blot results showed that the expression of protein Bcl-2, Cyclin B1 and Akt were decreased, which all showed close relationship with cell apoptosis, cell cycle and PI3K-Akt signaling pathway.The expression of PI3K was increased, but the change of PTEN was not statistically significant compared with that of control group.Conclusion Hesperidin induces lung cancer cell apoptosis through PI3K-Akt signaling pathway, which blocks cancer cell division and destroys the balance of related protein expression.

To meet the requirement of curriculum design about medical imaging field to undergraduate in medical college,the course of medical imaging radiation protection was developed by compiling a new textbook,establishing curriculum planning,implementing and evaluating teaching system.The teaching system of “one aim and two follows” was set up by use of three stages and three cycle feedbacks research and the course construction was promoted and developed by its teaching assessment,summary in time and the innovation of the teaching method.