Objective:To observe effects of Yiqi Yangyin Jiedu Fang on serum vascular endothelial growth factor(VEGF) and Immunologic function in the patient of non-small cell lung cancer(NSCLC).Methods:Fifty-one cases of NSCLC were randomly divided into a Chinese medicine group,a chemotherapy group and a chemotherapy plus Chinese medicine group.The therapeutic effect and changes of clinical symptoms,physical strength,immunologic indexes and serum VEGF before and after treatment were investigated in all groups.Results:Tumor stable rate in Chinese medicine group and chemotherapy plus Chinese medicine group after treatment was higher than that in chemotherapy group with no significant difference,but improvement of clinical symptoms and physical strength in Chinese medicine group and chemotherapy plus Chinese medicine group after treatment was significantly superior to that in chemotherapy group(both P

Objective To understand the endemic situation of schistosomiasis after its transmission interruption in Baoying County,so as to provide the evidence for adjusting the prevention and control strategy. Methods The data of schistosomiasis surveillance in Baoying County from 1988 to 2015 were collected and retrospectively analyzed. Results The schistosomiasis surveillance has been established since it was interrupted in Baoying County in 1987. The total surveyed area with Oncomelania hupensis snails was 21 269.95 hm2,but no living snails were found. The number of schistosomiasis examinations of residents was 163 196 person?times,and 270 person?times were positive in serum test,26 person?times were positive in stool test. No positive cases were found since 1997. The number of schistosomiasis tests of livestock was 2 180 cattle?times but no infection was found. Conclusion No schistosome infected snails or livestock are founded in Baoying County for last 28 years,and no patients are founded for last 11 years. The effect of schistosomiasis control is steady.

In view of the deficiencies of both Qi and Yin in most cases of this tumor, Jinfukang oral solution (JFK). a remedy mainly for benefiting Qi and nourishing -yin. was formulated. 96 cases were treated with one case of CR. 8 cases PR, 52 NC. the rate of PR + NC being 63. 5%. Another 52 cases were treated with chemotherapy +JFK. with 11 cases of PR, 26 cases NC, the rate of PR + NC being 71. 2%. While 25 cases were treated by chemotherapy alone, the cases of PR was 4; NC 26. the rate of PR + NC being 60. 0%, This demonstrated that the effect of JFK and JFK + chemotherapy were all superior to effect of chemotherapy alone. For 1 - and 2 - year survival rates, the JFK group was 67. 3% and 66. 7% respectively, whereas that of JFK + chemotherapy. 67. 3% and 66. 7%. For the chemotherapy group , 1 - year survival rate was 40. 3%, with no survivals within 2 years. For symptom improvement, body weight gaining, and health condition (KPS evaluation) after treatment, the JFK and JFK + chemotherapy groups were, again, superior to the chemotherapy group. The Immunity indices and blood picture in JFK group after treatment were markedly improved as compared with those before treatment, but, those in the chemotherapy group were decreased, while those of the JFK + chemotherapy group remained unchanged.

Immunosenescence is a biological process featured by the structural and functional decline of immunity in ageing people,which favors the carcinogenesis by rendering the body's immune surveillance and tumor escape.Decreasing clearance of tumor cells by macrophages and natural killer cell reflects the immunosenescence of innate immune,also,the tumor antigen presentation ability of dendritic cell is decreased.While,the decline of the CD8 + T cells cytotoxic effect and the secretion of tumor growth factor accounts for the immunosenescence of adaptive immunity.Intervention of immunosenescence may produce the novelty ideas of prevention and treatment of cancer.

Objective To explore the effects of DNMT3b on the expression and methylation sta-tus of the promoter region of DLC-1 in human hepatocellular carcinoma cell line. Methods The SMMC-7721 cell line was divided into 2 groups. The cell line in the experimental group was transfect-ed with DNMT3b siRNA, while that in the control group was transfected with control siRNA. West-ern blot was used to detect the expression of DNMT3b and DLC-1 and MSP was employed to examine the methylation status of the promoter region of DLC-1. Results The expression of DNMT3b was significantly higher in the experimental group than in the control group, while the expression of DLC-1 was just opposite. There was no significant difference in the methylation status of the promoter re-gion of DLC-1 between the 2 groups and both were methylated. Conelnsion The inhibition of expression of DNMT3b by siRNA method can enhance the expression level of DLC-1, and the methylation status of the promoter region of DLC-1 does not change at the same time. When affecting the expression of DLC-1, DN-MT3b might not play the role of methyhransferase, but can act as a transcriptional regulatory factor.

The distribution of somatostatin (SRIF) in substantia gelatinosa of the rat spinal cord was studied by means of immuno-electron microscopy. The ultrastructural features showed that nerve terminals containing SRIF take part in forming presynap- tie elements of axe-somatic, axe-dendritic and axo-axonic synapses. The immune- reactive products locatl at the external membrane of mitochondria, around the small clear synaptie vesicles and in the large granular vesicles. Most of synaptic vesicles are round or ovoid in shape. Only a few of them are flattened. Based on the ultrastructural characteristics mentioned above and related experimental results the authors believe that SRIF in substantia gelatinosa of the rat spinal cord is probably involved as a neurotransmitter instead of neuromodulator.

Objective To explore the expression and significance of transforming growth factor-?(TGF-?),epidermal growth factor receptor (EGFR) and proliferation-related antigen Ki-67 in nephroblastoma. Methods Immunohistochemistry method was used to detect the expression of TGF-?,EGFR and Ki-67 in 8 cases of normal kidney,35 cases of nephroblastoma and 14 cases of the tissues adjacent to nephroblastoma.The differences of their expression were compared among the 3 kinds of tissues and different pathologic characteristics of nephroblastoma. Results The expression rates of TGF-?,EGFR and Ki-67 in nephroblastoma were 51.4%(18/35),62.9%(22/35) and 68.6%(24/35),respectively,which were significantly higher than those in the tissues adjacent to nephroblastoma and the normal kidney;the differences were significant (P0.05);but positive relationship was found between the expression and clinical stages (P

Objective To study the effects of histone deacertylase inhibitor (TSA) on promoter methylation and expression of E-cadherin gene in a hepatocellular carcinoma cell line SMMC7721. Methods Hepatocellular carcinoma cell line SMMC-7721 was treated with TSA (300 nm/L), MTT method was used to investigate the growth inhibition ratio, TUNNL was conducted to measure the apoptosis ratio, methylation-specific PCR (MSP) was employed to detect changes in the CpG island methylation of E-cad promoter region, Western blot technique was used to detect the expression of E-cad gene and DNMT3b before and after TSA treatment, respectively. Results TSA decreases the SMMC-7721 cell viability and induces apoptosis, the growth inhibition ratio was 21.85% compared with control group. The apoptosis ratio of control group was (4.69±0.56)% ,the apoptosis ratio of TSA treatment group was (14.94±0.91)%. The apoptosis ratio of TSA treatment group was significantly higher than that of control group(P = 0.000). Before treated with TSA, the CpG island of E-cad promoter region was methylated, and the expression of E-cad was negative. TSA treatment induces demethylation of the CpG island in E-cad promoter region, causes the re-expression of E-cad. TSA reduces the expression of DNMT3b. Conclusions TSA decreases the SMMC-7721 cell viability and induces apoptosis, reverses the methylation status of E-cad promoter region, and resumes E-cad gene expression. TSA may induce demethylation through down-regulating the expression of DNMT3b.

Objective To explore the joints and tendons characteristic features of high frequency ultrasound in asymptomatic hyperuricemia(AH) and to assess its value in AH diagnosis.Methods One hundred asymptomatic individuals with hyperuricemia (AH group) and 75 normouricemic subjects (control group) were examined by high frequency and color Doppler ultrasound.The joints,tendons and soft tissue of the knees,ankles and first metatarsal-phalangeal joints in two groups were examined.The correlations of serum urate(SU) level with double contour sign,bone erosion and articular cavity effusion were analysed.Results Hyperechoic deposition on the sulface of the articular hyaline cartilage (double contour sign) were found in the knees (35),ankles (17) and the first metatarsophalangeal joints (MTPJs,48) from the asymptomatic individuals with hyperuricemia,in contrast to none in the control group (P ＜0.05).Similar results were found in two groups of the bone erosion (30,25,20 vs 4,6,3,P ＜0.05),the effusion (28,36,41 vs 6,8,9,P ＜0.05) and the hyperechoic spots (19,18,22 vs 0,0,0,P ＜0.05).Patellar enthesopathy (4.5％ vs 0.6％,P ＜0.05) as well as achilles enthesopathy(8％ vs 0,P ＜0.05) and achilles tophi (6％ vs 0.6％,P ＜ 0.05) were more frequent in asymptomatic hyperuricemic than that of normouricemic individuals.No correlations between SU concentration and the presence of the double contour sign,the bone erosion and effusion were found (P ＞0.05).Conclusions There were some similar specific sonographic findings of gouty arthritis in the asymptomatic individuals with hyperuricemia.Also,these findings support high frequency ultrasound as a useful tool to detect anatomical damage in the joints,synovial tissue and tendons of asymptomatic individuals with hyperuricemia.

Objective To assess the prediction value of nonbronchial systemic arterial supply in hemoptysis patients with non-enhanced CT. Methods Fifty-six consecutive patients with hemoptysis underwent non-enhanced CT. Thickness of pleural adjacent to parenchymal lesion larger than 3 mm was regarded as index of nonbronchial systemic arterial supply. Conventional angiography was used as the standard of reference. CT findings were compared with those of conventional angiography. The sensitivity, specificity, and accuracy of CT for predicting nonbronchial systemic arterial supply were assessed. Results The sensitivity, specificity and accuracy of CT for predicting nonbronchial systemic arterial supply were 72.73%, 95.00% and 91.11%, respectively. Sensitivity was higher when nonbronchial systemic arterial supply located in superolateral and posterolateral lung, and lower in anteromedial and inferior lung. Specificity and accuracy were high for predicting nonbronchial systemic arterial supply in every locations. Conclusion Non-enhanced CT can predict nonbronchial systemic arterial supply in patients with hemoptysis, which is helpful for selecting angiography and embolization.