This study compares and analyzes the similarities and differences between National Health Strategy in America and Healthy China 2030 from three aspects, namely the strategic goals, the main contents, and the leading health indicators.Considering China's social and economic environment, this paper proposes the following suggestions: 1) to emphasize the relevance, dynamics and continuity of health strategy;2) to focus on key population on the basis of full coverage;3) to identify the focus of each phase of implementation;and 4) to highly think of the establishment of systems, including information system, operational supervision system and evaluation index system, etc.These suggestions are meant to provide inspiration to the introduction and further development of the China's national health strategy.

Objective To analyze the equity of the allocation of the health human resources in Chongqing from 1997 to 2012 , and to provide the basic information for regional health planning .Methods The statistical description ,Gini Coefficient and Theil in-dex are used to analyze the previous allocation and trends of the health human resources and the equity in Chongqing during the 16 years based on the distribution of demography and geography .Results The number of the health human resources grew rapidly from 1997 to 2012 .The Gini coefficients of health professionals and medical practitioners (and assistants) are under 0 .3 based on the distribution of demography ,while registered nurses′are fluctuation in 0 .4 .However ,from the distribution of geography ,the Gi-ni coefficient of health professionals ,medical practitioners (and assistants) and registered nurses are above 0 .5 .The Gini coefficient of the one hour economic circle is higher than two wings areas .The trends about the totle Theil index are consistent with the Gini coefficient .Based on the distribution of demography ,the Theil index between regions is higher than the one within the region .On the contrary ,the Theil index between regions is below the one within the region by the geographic distribution .Conclusion Com-pared to the national ,less total health human resources are reserved in Chongqing .Distribution by demographics about health human resources are more equitably than the one by geographic distribution .The variances between the economic circles contribute more to the total Theil index than those within the economic circles .The government′s leading role should be strengthen ,and enhancing the regional health planning .

Objective:To establish a paired organoid culture system for primary pancreatic cancer lesions and liver metastatic lesions in mice, and to investigate their morphological and biological behaviors.Methods:C57BL/6 mice aged 6 to 8 weeks were selected. Pancreatic cancer PANC02 cells carrying luciferase were injected into the pancreatic tail. After monitoring the formation of liver metastases using an in vivo imaging system, mice were sacrificed, and paired primary pancreatic cancer lesions and liver metastatic lesions were extracted and cultured in an in vitro organoid culture system. The formation process of organoids was observed under an inverted phase-contrast microscope. Hematoxylin and eosin staining was used to examine the morphological structure of the organoids. The expression of epithelial cell marker CK19 and proliferation marker Ki67 in the organoids was detected by immunohistochemistry and immunofluorescence staining. The expression of invasion markers N-cadherin, E-cadherin, vimentin, snail, and MMP9 was assessed by Western blotting and immunohistochemistry. The drug sensitivity of organoids to gemcitabine was evaluated using the CellTiter-Glo ? 3D Cell Viability Assay, and the half-maximal inhibitory concentration (IC 50) of the organoids was calculated. Results:A spontaneous liver metastasis model of pancreatic cancer in mice was successfully established, along with a paired organoid culture system for primary pancreatic cancer lesions and liver metastatic lesions. The organoids grew in a spherical shape and could be passaged up to 10 generations in vitro. Both liver metastatic lesion organoids and primary pancreatic cancer lesion organoids exhibited lumen-like structures, expressed the epithelial cell marker CK19, and the proliferation marker Ki67, with a significantly higher positive ratio of Ki67 in the liver metastatic lesions compared to the primary pancreatic cancer lesions. The expression levels of invasion markers N-cadherin, vimentin, snail, and MMP9 were significantly higher in liver metastatic organoids than in primary pancreatic cancer organoids, whereas the expression level of E-cadherin was significantly lower in liver metastatic organoids. The IC 50 value of gemcitabine for liver metastatic organoids was 165.0 nM, higher than the 108.5 nM for primary pancreatic cancer organoids. Conclusions:A stable, passagable organoids of primary pancreatic cancer lesions and liver metastatic lesions in mice were successfully established.

This paper reviewed international documents of disability statistics. The results showed that all disability measurements had been adopted International Classification of Functioning, Disability and Health (ICF) terminology and approach. Disability statistics in US tailored to ICF framework and classification. Disability statistics in China had partially adopted ICF approach.