Objective To investigate serum leptin levels in maintenance hemodialysis (MHD) patients with chronic renal failure and the relationship between serum leptin levels and nutriture.Methods After hemodialysis,the brachial triceps skin fold was determined in MHD patients,then counted the body mass index(BMI) Serum leptin levels of 55 MHD patients and 40 normal controls were measured by radioimmunossay (RIA) .Simultaneously, other biochemical parameters;BUN, creatinine, serum albumin, triglyceride, cholesterol ,HLD,LDL etc also were mensured.Results ⑴Serum leptin levels in MHD patients were significantly higher than that in normal controls(P

Objective The goal of the present experimental study is to investigate effects on the injection of a-dult rabbit autologous skeletal myoblasts into the thyroarytenoid(TA)muscle after recurrent laryngeal nerve (RLN) damage and traumatic vocal cords .Methods 20 adult New Zealand white rabbits were used for the establishment of an animal model simulating the cut -off of the left recurrent laryngeal nerve and the left vocal cord trauma scar for-mation after traumatic damage .Then from the rabbits ,the sternocleidomastoid muscle cells were cultured ,separa-ted and expanded .After 12 weeks ,the 20 animals were randomly divided into group A ,group B with 10 in each group .Group A(myoblast injection group) in the left vocal cord thyroarytenoid muscle injection of myoblasts 0 .3 ml ,the right vocal cord without any treatment served as normal controls ;Group B(saline injection control group) in the left vocal cord thyroarytenoid muscle was injected with normal saline ,the right vocal cord without any treatment as normal controls ,respectively ,were observed after 12 weeks of bilateral thyroarytenoid muscle fiber diameters and vocal cord volumes .Results After 12 weeks ,Group A's left thyroarytenoid muscle injection of bilateral thyroaryten-oid muscle fiber diameter and the vocal volume size was not significantly different (P>0 .05) ,thyroarytenoid muscle fiber diameter and the vocal cord volume on Group B's injected side was significantly smaller than Group A (P<0 .05) .Conclusion The thyroarytenoid muscle injection of autologous myoblasts can effectively treat recurrent la-ryngeal nerve (RLN) injury and traumatic vocal cords .

Objective:To evaluate the short-term economic effects of four kinds of premixed insulin in newly diagnosed type 2 dia-betes mellitus. Methods:A total of 120 newly diagnosed patients with type 2 diabetes mellitus were divided into four groups according to the kind of premixed insulin, group A was treated with insulin aspart 30 injection, group B was treated with insulin lispro 25 injec-tion, group C was treated with isophane protamine biosynthetic human insulin injection and group D was treated with protamine zinc re-combinant human insulin injection. The course of treatment was three months. The therapy efficacy was assessed by the remission rate in three months. The short-term economic effect was evaluated by the cost-minimization analysis method. Results:The remission rate of group A, B, C and D respectively was 48. 39%, 48. 28%, 51. 61% and 51. 72% without significant difference (P>0. 05). The average cost per person of the four groups was 1195. 52, 1202. 41, 1220. 69 and 1258. 84 yuan, and the average medicine cost per person was 750. 52, 689. 41, 754. 69 and 764. 34 yuan, respectively. There was no significant difference in cost among the four groups (P >0. 05). Conclusion:All the four kinds of premixed insulin can be used for the starting treatment with the similar total cost, and in relative terms, aspart 30 injection and insulin lispro 25 injection are better for the initial treatment of diabetes.

Since the fecal bacteria transplantation (FMT) had been exist for a long time, it attracted more attention in recent years because this therapy could adjust the bacteria disturbance rapidly in clinical settings. Recent studies demonstrated that fecal microbiota transplantation (FMT) has great clinical value in the treatment of Clostridium difficile infection, inflammatory bowel disease, Intractable functional constipation and other diseases. But the FMT is not as perfect as we expect, so we did this article to review the history and terminology of FMT, and summarized the progress in nursing methods of FMT.

BACKGROUND: Nerve growth factor is secreted and synthetized by a variety of cells, such as inflammatory calls and repairing calls, its biological effects are diverse and closely related to the process of wound repair, but its mechanism is not yet clear.OBJECTIVE: To observe the influence of nerve growth factor on the biological characteristics of scar fibroblasts.METHODS: Eight clinical surgical resection specimens, including 5 face and neck hyperplastic scar or keloid specimens, did not receive any treatment; three were prepuce specimens following circumcision (normal tissue). By use of tissue block method, the scar and normal skin fibroblasts were cultured, followed by digestion passage. The scar tissue and normal tissue flbroblasts at 3-6passages in the logarithmic phase were seeded in 96-well plate and divided into the experimental group (scar flbroblest group) and the control group (normal skin fibroblasts group), with two parallel holes in each group were added with 3,33, 0.33 mg/L nerve growth factor, 50 μL. Inverted microscope was used to observe fibroblast morphology. At 24, 48, 72 hours after culture, the absorbanca value was measured using MTT. Fibroblast DNA content and cell apoptosis were determined by flow cytometry.RESULTS AND CONCLUSION: The fibroblasts were adherent cells, the scar and normal skin tissues were shown to cell free out of tissue block and gradual expansion at 4-6 days after incubation. Compared with normal skin fibroblasts, the pathological scar fibroblasts became larger, irregular shape and arrangement. MTT results showed that nerve growth factor could promote the normal and hypertrophic scar fibroblasts growth, which becomes more apparent. Flow cytometry results showed that by adding nerve growth factor, the percentage of scar fibroblasts at proliferating S-G_2-M phase was higher than that in the control;group; with a Iower level of apoptosis. It is indicated that nerve growth factor plays an obviously promoting role on normal and scar skin fibroblasts growth and proliferation, especially on the scar skin.

Objective To explore the mechanism of cytokines for the scars,and to study the effect of platelet derived growth factor(PDGF)on the biological behavior of fibroblasts in scars.Methods Fibroblasts of scars and normal skins were cultured in vitro.The results were observed and analyzed by light inverted microscopy(LM),and 3-(4,5-dimethyl-thiazol-2-yl)-2,5 ciphenyl tetrazolium bromide (MTT)assay.The effects of PDGF on the biological behaviors of fibroblasts of scars were also determined. Results In vitro study,using LM,FCM and MTT assay,showed that proliferation of fibroblasts were inereased significantly when PDGF was added to the cultures,as compared to the control groups.Conclusions PDGF can increase fibroblast proliferation.These results demonstrate that PDGF is beneficial for wound healing at early stage.

AIM: To study the prognositic value of PTEN and Her-2 expression in primary breast cancer.METHODS: 81 breast cancer specimens with 15 years follow-up were obtained from 1989 to 2004.Immunohistochemical methods were used to detect the expression of PTEN and Her-2 in 81 paraffin-embedded specimens.The correlation between expression of PTEN and clinipathological factors was discussed with the Chi-square test.The survival rate analysis results were calculated with Kaplan-meier method.Long-rank test and Cox model by SPSS 10.0 software.RESULTS:(1) PTEN expression significantly affects 5-year and 10-year survival rate of breast cancer(P

With the increasingly strengthening of economic globalization and higher education internationalization,medical imageology as an important intersecting science in life sciences has more and more international communion and cooperation.In China many hospitals and medical colleges require large quantities of medical imageology professionals,and it is very necessary and important to develop the bilingual education in medical imageology,though a series of problems appear in the process.

The developing situation of "big image" makes the medical imaging become an increasingly huge and complicated system.It offers higher request to the medical imaging teaching.The internship of last term is always influenced by attending the graduate student's entrance examination and looking for job,the effect of teaching can't be assured,and it especially important to do well in field trip,thus it can reduce or avoid the phenomenon of "practice after graduate".

With development of medical imaging,especial the wide application of CT,MRI and so on,traditional education method can not meet the needs of the intern-clinical students.Fairly satisfying teaching effects has been achieved for the effective combination of traditional teaching methods with net-work educational method.