Ischemia and reperfusion of rat hearts were produced by Langendorff per-fusion technique. The energy tranduction of the mitochondria isolated from the rat heartswas studied. The initial rate of proton ejection and eldectron transfering were measured.There was no significant difference of H~+/2_e ratio between the ischemic group and thecontrol group which was reduced only in the reperfusion group. The longer was the is-chemic period before reperfuaion, the smaller would be the H~+/2_e ratio. The lowering ofH~+/2_e ratio occurred earlier than that of the ADP/O ratio. RCR and ADP/O in shortperiod ischcmia group and reperfusion group were both much higher than that of the con-trol group.

Objective To study the interaction of genetic polymorphisms of CYP2E1 and environmental hazard factors in gastric cancer. Methods 1∶1 case-control study was carried out in Nanjing area, using PCR-RFLP technology to determined the genetic polymorphisms of CYP2E1 and epidemiological data about environmental exposure of 121 cases and controls were collected. Interaction indexes (?) were calculated to determine the type of gene-environment interaction. Results After confounding adjusted, the result showed that interaction existed in genetic polymorphisms of CYP2E1 RsaⅠand the family history of digestive system disease, smoking, pickled food, irregular diet habit, interaction indexes (?) value were 1.855, 2.626, 1.736 and 1.714 respectively. A low exposure-gene effect interaction was found in CYP2E1 RsaⅠgenotype and the frequency of pickled food consumed, while a high exposure-gene effect interaction exist in CYP2E1 RsaⅠgenotype and irregular diet habit. Conclusion Genetic and environmental hazard factors co-effect the development of gastric cancer, genetic polymorphisms of CYP2E1 and environmental risk factors show an interaction in gastric cancer.

Objective To investigate serum levels of β-human chorionic gonadotrophin (β-HCG) and progesterone (P) in early pregnant women,and their relation to luteal maintenance therapy for early pregnancy protection.Methods One hundred and thirty five infertility women treated in Department of Gynecology and Obstetrics of Beijing Chaoyang Hospital from January 2007 to December 2011.Total 150pregnancy cycles,including 84 with intrauterine insemination (IUI) and 66 with natural conception,were divided into two groups:normal intrauterine pregnancy group (group A,n =80) and early pregnancy loss group (group B,n =70).Medical history,ultrasonic findings,serum female hormone,P and β-HCG levels at early pregnancy stage were analyzed.Results The age of group A and group B was (30.0 ± 3.9) years and(30.7 ± 4.9) years,respectively (P ＞ 0.05).The follicle-stimulating hormone/luteinizing hormone in group A and group B was 1.57 ±0.96 and 1.56 ± 1.08 ; the estradiol levels in two groups were (152 ±66) pmol/L and (147 ± 69) pmol/L,respectively (both P ＞ 0.05).There were no differences in dominant follicles and endometrial thickness between groups A and B (P ＞ 0.05).Ovulation promotion and luteal support treatments were adopted in both groups:50％ (40/80) of cycles in group A received ovulation promotion,73％ (58/80) of cycles received luteal support,while 44％ (31/70) and 76％ (53/70) received in groups B,respectively.The levels of serum progesterone in group A during 14-21 d,22-27 d and ≥ 28 d after ovulation were higher than those in group B at each time points (P ＜ 0.0l).There were no significant differences in serum progesterone levels between women with luteal support treatment and those without luteal support treatment in both groups (P ＞ 0.05).Conclusion Dynamic monitoring of serum progesterone level in early pregnancy may be used as an auxiliary index for prediction of pregnancy outcome,but it may not be an indicator for luteal maintenance therapy.

Objective: To explore the troubleshooting of medical equipment and ensure its function and performance in daily work in hospital. Methods: According to the fault degree, equipment important degree, time and funds the difference, we should adopt different repair method. Results:Medical equipment fault is varied. The repair method depends on the repair time, repair funds for various reasons, we should according to the specific situation, weigh the advantages and disadvantages before making a decision. Conclusion:Medical equipment repair involves many factors. Leadership should be aware of the engineering and technical personnel in the hospital daily work, improve the level of engineering and technical personnel, in order to safeguard the medical equipment intact rate and meet needs.

Objective To evaluate the relationship between thyroid autoantibodies (ATA)and pregnancy outcome of infertility women after assisted reproductive technology (ART). Methods Relevant studies were identified by searching PubMed, Cochrane library, CNKI and CBM. Results 7 studies were appropriate for meta-analyses and total 4165 patiens were included. Meta analysis shows that the pregnancy rate difference between ATA positive patients and controls have no significance, but the miscarriage rate is greater significant between them. In the meta-analyses, the presence of thyroid antibodies was associated with an increased risk of miscarriage (OR 1.85, 95%CI 1.32~2.59), compared with the absence of thyroid antibodies. Conclusion Pregnant women with thyroid antibodies have an increased risk of complications, especially miscarriage. Future research,should focus on the treatment and thyroid disease on pregnancy outcome.

Objective To investigate the value of bronchoscopy in the etiologic diagnosis and therapy of right middle lobe atelectasis. Methods Clinical data of 45 cases of right middle lobe atelectasis under fiberoptic bronchoscopy from January 2012 to February 2016 were analyze retrospectively. Results 28 cases (62.2 %) were determined inflammation, 9 cases (20.0 %) of tumor, 4 cases (8.9 %) of tuberculosis, 1 case (2.2%) of foreign body, 3 cases (6.7 %) unexplained. After treatment, 30 cases (66.7 %) were cured, 8 cases (17.8 %) improved while 7 cases (15.5 %) invalid. Conclusions The bronchoscopy is a critical technology for diagnosis and therapy of right middle lobe atelectasis.

OBJECTIVE:To observe the stability of fleroxacin injection. METHODS: The contents of fleroxacin injection and its degradation product were determined by HPLC method,and the effects of light, heat, pH value and halogen ion on the stability of fleroxacin injection was also studied. RESULT: The average recovery rate of the fleroxacin was 100.4%(RSD=0.87%); the color of fleroxacin injection deepened and the content of the degradation product increased if its pH value was lower or if the illuminating or heating time became longer; the sediments were separated out if met with halogen ion. CONCLUSION: The fleroxacin injection was unstable and easy to decompose under light and heat, especially under the light; the content is more likely to be decomposed if the pH value is lower;fleroxacin injection can not to be applied together with the halogen ion.

The incidence in 7768 cases of acute craniocerebral injuries was analysed in this article.The analysis showed that there were more cases of acute craniocerebral injuries seen during the period from June to November,especially from June to August.The main causes of injury from June to August were trips and falls (41.7%) and those from September to November were traffic accidents (48.7%).Improvements in rescue orga- nization and measures in prevention during the period of high incidence were sugge sted.

Objective To explore the antimicrobial resistance of Moraxella Catarrhalis in children with respiratory infection.Methods Eleven strains of Moraxella Catarrhalis were isolated from nasopharyngeal secretion in 145 children with community-acquired respiratory tract infection in Beijing Friendship Hospital from 2004 to 2006.Segregated stocks were isolated from upper respiratory infection in 5 cases,bronchitis in 3 cases and pneumonia in 3 cases.Agar dilution method was used to determine minimal inhibition concentrations (MIC),including 8 kinds of antibiotics,and ?-lactamase was detected.WHONET 5 and SPSS 11.5 software were used to analyze data.Results Ten of the 11 strains were ?-lactamase positive.The rates of resistance to ampicillin,cefuroxime and erythromycin were 81.8%,63.6% and 18.2% respectively,however,all the strains were susceptible to ceftriaxone.MIC90 of penicillin and cefradine was 32.0 mg/L and 8.0 mg/L respectively.MIC90 of roxithromycin and azithromycin was 2.0 mg/L and 0.25 mg/L respectively.Conclusions Moraxella Catarrhalis is an opportunistic pathogen.The ?-lactamase positive rate of Moraxella Catarrhalis from children is high,and there is also a high resistance percen-tages of Moraxella Catarrhalis to penicillin,ampicillin,first and second generation cephalosporins.Moraxella Catarrhalis is susceptible to Cefuroxime.

Chronic inflammatory demyelinating polyneuropathy (CIDP) is one of the acquired autoimmune peripheral neuropathy with various therapeutical methods. This article reviewed the therapeutical advances in CIDP.