Childhood asthma and obesity are significant public health problems all over the world.In the past decades,the prevalence of both diseases has increased considerably.Because both asthma and obesity appear to have their beginnings in early childhood,common exposures that predispose individuals to both conditions may explain how they are associated.These exposures include common genetic predictors,prenatal exposure to specific nutrients and overall maternal nutrition, colonization patterns of the neonatal and infant gut,birth weight and infant weight gain,and sedentary behaviors in early life.

Objective To investigate the freatment outcome of internal fixation of the metacarpal and phalanx fracture using the pure fitanium mini plate.Methods 42 cases(78 lesions) of metacarpal and phalanx fracture were involved.Internal fixation with custom made pur titanium mini plate was done.Results Postoperative follow up rauged from 3 to 14 months and the fracture was healed in 42 cases.The bone union of roantgenographic eridence was 6 to 12 weeks.According to the functional evaluation of TAM ascles the excellent and good rate was 85.7%.Conclusion The AO mini-plate in treatment of the metacarpal and phalax fracture has a firm fixation.It is an optimal strategy for metarcarpal and phalanx fracture.

Objective To study the chemical constituents in the seeds of Euphorbia lathyris.MethodsCompounds were isolated by methods of column chromatography(silica gel,including reversed phase),Sephadex,and recrystallization.On the basis of spectroscopic methods including IR,MS,NMR,and X-ray,structures of compounds were confirmed.Results Twenty-two multi-type compounds were isolated from ethanol extract in the seeds of E.lathyris.Their structures were identified as 5,15-O-diacetyl-3-O-phenyl-6(17)-epoxylathyrol(1),5,15-O-diacetyl-3,7-O-dibenzoyl-7-hydroxylathyrol(2),5,15-O-diacetyl-3-O-benzoyl-lathyrol(3),20-O-hexadecanoyl-ingenol(4),3-O-hexadecanoyl-ingenol(5),15,17-O-diacetyl-3-O-cinnamoyl-17-hydroxyjolkinol(6),5,15,17-O-triacetyl-3-O-benzoyl-17-hydroxyisolathyrol(7),5,15-O-diacetyl-3-O-nicotinoyl-lathyrol(8),5,15-O-diacetyl-3-O-benzoyl-7-O-nicotinoyl-7-hydroxy-lathyrol(9),ingenol(10),lathyrol(11),esculetin(12),?-sitosterol(13),benzene-1,2,3-triol(14),palmiticacid(15),2,3-dihydroxypropyl icosanoate(16), 2,3-dihydroxypropyl oleate(17),2,3,4-trihydroxybutyl hexadec-3-enoate(18),aurantianide acetate(19),benzoic acid(20),p-hydroxybenzoic acid(21),oleic acid(22).Conclusion Among these,compounds 10,11,1419 are obtained from this plant for the first time and compounds 1-3 are the main diterpenes.

Objective To explore the apoptosis of K562/G01 cells induced by triptolide through MDM2/p53 signaling pathway. Methods K562/G01 cell line was treated with different concentrations of triptolide. MTT was used to detect the cell proliferation inhibition rate. FCM was used to determine the apoptosis rate changes in 12 h and 24 h. The mRNA expression levels of bcr-abl, XIAP, MDM2, p53 were detected by real-time quantitative PCR. Results After treatment by 10, 20, 40, 80, 100 nmol/L TP in 12, 24, 48 h, the viability of K562/G01 cells was inhibited in time-dose dependence manner. K562/G01 cells was treated by 20 nmol/L, 40 nmol/L TP after 12 h, 24 h, the cell apoptosis rate was rising with drug concentration and time. The bcr-abl, XIAP, MDM2 mRNA expression was down-regulated and p53 mRNA expression was up-regulated by TP. Conclusion TP can inhibit the growth of K562/G01 cell line and induce apoptosis through XIAP-MDM2-p53 signaling pathway.

BACKGROUND: Osteogenic growth peptide (OGP) is a newly discovered growth factor to promote osteogenesis, the effect on proliferation and differentiation of bone marrow stromal cells (MSCs) arouses more and more attention.OBJECTIVE: To elucidate the effect of OGP on proliferation and differentiation of MSCs in vitro.DESIGN, TIME AND SETTING: A randomized COntrol experiment was performed at the Key Laboratory for Oral Biomedical Engineering by the State Ministry of Education from February to June 2006.MATERIALS: A 6-month-old male Sprague-Dawley rat was used for the extraction of MSCs; and OGP was provided by Sigma Company, USA.METHODS: MSCs were cultured individually in a-MEM medium containing various COncentrations of OGP (10-11 mol/L,10-10 mol/L, 10-9 mol/L, 10-8 mol/L and 10-7 mol/L) and fetal bovine serum. The cells in the control group were cultured with α-MEM medium containing FBS alone.MAIN OUTCOME MEASURES: Identification of MSCs; cell proliferation using MTT method; alkaline phosphatase (ALP) activity using enzyme-linked immunosorbent assay; core binding factorα1 (Cbfa1) mRNA expression using RT-PCR.RESULTS: The addition of OGP resulted in an increase of cell proliferation (10-9mol/L OGP, P < 0.05), ALP activity (10-8 mol/LOGP, P < 0.05) and Cbfal mRNA expression (P < 0.05).CONCLUSION: OGP stimulates proliferation and sequential differentiation of MSCs via inducing Cbfal mRNA expression.

BACKGROUND: Microspheric injectable scaffold has a perspective in cartilage tissue engineering; however, it is still limited by in vivo hard forming and microsphere transmigration.OBJECTIVE: To investigate the feasibility of self-assembly scaffolds by attaching negatively charged calcium alginate microspheres and positively charged chitosan microspheres by electrostatic force. METHODS: The calcium alginate and chitosan microspheres were prepared by emulsion-internal gelation and spray drying technique, respectively. The characterizations of the microspheres were determined by means of scanning electron microscopy, optical microscopy and zeta potential analysis methods. Self-assembly scaffolds were fabricated by mixing the aqueous suspensions of the microspheres with oppositely charged surfaces. The phenomenon of electrostatic attachment was characterized by optical microscopy and scanning electron microscopy, and the elastic compress modulus of the scaffolds was also investigated.RESULTS AND CONCLUSION: The average diameter of the calcium alginate microspheres was 52.5 μm, and the chitosan one was 4.1 μm, respectively. The zeta-potential of the calcium alginate microspheres was 23.5 mv, and the chitosan one was +9.8 mv, respectively. The microspheres were spherical and smooth. The small size chitosan microspheres could attach to the surface of the calcium alginate microspheres and anchor the calcium alginate microspheres together. The elastic compress modulus increased with the increase of solid content of the microspheres, but decreased with the increase of the ionic strength. The elastic compress modulus increased firstly and then decreased with the increase of the mass ratio of m (CHI):m (ALG), and it showed the highest elastic compress modulus when m (CHI):m (ALG) was 2:1. The positively charged chitosan microspheres could attach to the negatively calcium alginate microspheres to form a self-assembly scaffold.

ABSTRACT:Objective To investigate the regulation of adenovirus type 36 infection on precursor protein particles progranulin expression in Uygur obese patients.Methods Based on the diagnostic criteria of obesity,the samples were divided into obese group and non-obese group.Serum neutralization test was used to detect the antibody of Ad36.The progranulin mRNA expressions in abdominal omental and subcutaneous adipose tissues were detected by real-time quantitative PCR.ELISA method was used to determine serum progranulin protein levels. CD68 protein expression of macrophage was detected by immunohistochemistry. Results ① In the Uygur population of our study,compared with that in the non-obese group (38/1 1 7,32.5%),the number of obese patients (54/1 1 5,47.0%)infected with Ad36 was significantly higher than that in non-obese group (P <0.05 ).② Serum progranulin was significantly increased in the Ad36-infected obese group (408.45±1 56.92)than in non-obese group (326.1 1±1 58.60)(P <0.05).The mRNA expression of progranulin did not differ between the two groups.③ The macrophage infiltration was significantly higher in the Ad36-infected obese group (14 730.1 6 ± 2 227.39 )than in non-obese group (10 786.50 ± 2 772.80 )(P < 0.05 ).Conclusion Ad36 infection may be associated with the occurrence of obesity in Uygur population,and adenovirus type 36 infection may regulate the expression of serum progranulin at the protein level.

Objective To compare clinical biochemical and pathological characters among antimitochondrial antibody-negative primary biliary cirrhosis (AMA PBC),AMA positive PBC (AMA+ PBC)and autoimmune hepatitis (AIH),and try to find serum biomarkers in the diagnosis of AMA-PBC.Methods From January 2005 to May 2013,23 patients with AMA-PBC,102 patients with AMA+ PBC and 55 patients with AIH were collected.Clinical features of patients were observed.Biochemical indexes (total bilirubin (TBil),direct bilirubin (DBil),alanine aminotransferase (ALT),aspartate transaminase (AST),alkaline phosphatase (ALP),γ-glutamyltransferase (GGT) and globulin) were tested.Immunological indexes immunoglobulin ((Ig)G,IgM and IgA) were also measured.Antinuclear amibody,anti smooth muscle antibody (SMA) and AMA were detected by indirect immunofluorescence.Soluble acidic phosphorylation nucleoprotein antibody and anti-nuclear membrane glycoprotein antibody were detected by Western blotting.Non parameter test was for non normal distributed measurement data and grade data comparison.The cut off value,sensitivity and specificity of IgM in the diagnosis of PBC were calculated with receiver operating characteristic (ROC) curve.The sensitivity and specificity of soluble acidic phosphorylation nucleoprotein antibody and anti-nuclear membrane glycoprotein antibody in the diagnosis of PBC were analyzed with fourfold table.Results The rates of fatigue and jaundice of AMA-PBC group (39.1％,9/23 ; 43.5 ％,10/23) were both higher than those of AIH group (16.4 ％,9/55; 14.5 ％,8/55),and the differences were statistically significant (x2 =4.735 and 7.648,both P＜ 0.05).The levels of ALP and GGT of AMA PBC group were higher than those of AIH group,and the levels of ALT and AST were lower than those of AIH group (Z=-4.577,-4.257,-2.820 and -2.055,all P＜0.05).The level of IgM of AMA PBC group (417(270,610) mg/L) was higher than that of AIH group (97(69,195) mg/L),however lower than that of AMA+ PBC group (546(419,704) mg/L),and the differences were statistically significant (Z=-4.362 and-0.210,both P＜0.05).The level of IgG of AMA PBC group was lower than that of AIH group (Z=-2.202,P＜0.05).The positive rates of antinuclear antibody and SMA of AMA PBC group (95.7％ (22/23),8.7％ (2/23)) were both higher than those of AMA+ PBC group (33.3％(34/102),1.0％(1/102)),and the differences were statistically significant (x2 =29.474 and 4.769,both P＜0.05).The positive rates of soluble acidic phosphorylation nucleoprotein antibody and anti nuclear membrane glycoprotein antibody of AMA PBC group (60.9％,14/23; 30.4％,7/23) were both higher than those of AIH group (2.0％,1/55; 0),and the differences were statistically significant (x2=36.409 and 24.329,both P＜0.05).The karyotype of antinuclear antibody of AMA PBC and AMA+ PBC group mainly was granules pattern (60.9 ％,14/23;75.5％,77/102),however of AIH group was mainly homogeneous pattern (38.2％,21/55).If the cut off value of IgM was 277 mg/L,the sensitivity and specificity of IgM in the diagnosis of PBC was 72.2％ and 94.4 ％.The sensitivity and specificity of soluble acidic phosphorylation nucleoprotein antibody in the diagnosis of PBC was 24.1％ and 97.2％.The sensitivity and specificity of anti-nuclear membrane glycoprotein antibody was 35.2 ％ and 100.0 ％.The sensitivity and specificity of any one of anti-nuclear membrane glycoprotein antibody positive,soluble acidic phosphorylation nucleoprotein antibody positive or IgM＞277 mg/L in the diagnosis of PBC was 88.9％ and 94.4％.Conclusions AMA-PBC and AMA+ PBC have similar clinical manifestations and serum biochemical test results.Antinuclear antibody and/or SMA positive is the feature of AMA-PBC and which should be distinguished with AIH.The sensitivity and specificity of any one of the followiags:anti nuclear membrane glycoprotein antibody positive,soluble acidic phosphorylation nucleoprotein antibody positive or IgM＞277 mg/L is high in the diagnosis of PBC.

OBJECTIVE:To observe the efficacy and safety of domestic cefepime in the treatment of patients with acute exacerbation of chronic obstructive pulmonary diseases (AECOPD) complicated with respiratory failure. METHODS:A total of ninety-six patients with AECOPD complicated with respiratory failure were randomly assigned to receive cefepime 2.0 g bid iv gtt (Group A,n=48) or cefoperazone/sulbactam (Group B,n=48) at a dose of 2.0 g bid iv gtt for 7～14 day. RESULTS:The effective rate was 82% for Group A versus 62% for Group B(P0.05). CONCLUSION:The efficacy of cefepime is higher than that of cefoperazone/sulbactam in the treatment of patients with AECOPD complicated with respiratory failure,but both had good safety.

OBJECTIVE:To evaluate the cough-relieving and sputum-eliminating efficacy and safety of Guaifenesin Pseudoephedrine Hydrochloride-Codeine Phosphate Oral Solution.METHODS:By a randomized control study,a total of 60 patients characterized by nonproductive cough andlittle sputum were randomly assigned to receive Guaifenesin Pseudoephedrine Hydrochloride-Codeine Phosphate Oral Solution(Trial Group)or Ambroxol Hydrochloride Oral Solution(Control Group)l0 mL tid for 5～7 d.RESULTS:Both groups had a significant improvement in symptoms including cough and expectoration after treatment(P0.05).CONCLUSION:Guaifenesin Pseudoephedrine Hydrochloride-Codeine Phosphate Oral Solution is more effective in relieving cough than Ambroxol Oral Solution,and it has certain effect in eliminating phlegm.In addition,it is safe and has little adverse reaction.