Objective Distortion product otoaconstic emission (DPOAE) was compared withauditory brain－stem response (ABR) in the early diagnosis of gentamicin (GM) ototoxicity. Whetherimpaired hearing could be improved with the glutathione treatment after its withdrawl of injection.Methods40 guinea pigs with normal hearing were randomly divided into 4 groups, 10 in DPOAEgroup, 12 in ABR I group (the usage of GM was stopped when abnormal shift presented in ABR), 12 inABR Ⅱ (group glutathione was injected for 5 after the usage of GM had been stopped), 6 in controlgroup. The guinea pigs in experimental group were injected with GM 100mg/kg per day, while those inthe control group were injected with saline. The DPOAE and ABR were monitored to observe the ampli-tude and the threshold of wave IV. The morphological changes of hair cells were also observed after injec-tion of GM had been stopped for 2 weeks. ResultsIt was averaged at 7d after the injection of GMwhen the changes presented in DPOAE group, while 10d in ABR group. The threshold shift increasedafter the injection had been stopped for 2 weeks in ABR Ⅰ group ( P <0.01) ,while there was no obvi-ous shift in ABR Ⅱ group. The morphological and functional changes of the hair cells were almost thesame. Conclusion GM ototoxicity could be more sensitively observed by DPOAE than ABR. Glu-tathione could prevent further ototoxic effect of GM to the cochlear.

Objective To study the cumulative radiation dose of multiple trauma patients during their hospitalization and to analyze the dose influece factors.Methods The DLP for CT and DR were retrospectively collected from the patients during June,2009 and April,2011 at a university affiliated hospital.The cumulative radiation doses were calculated by summing typical effective doses of the anatomic regions scanned.Results The cumulative radiation doses of 113 patients were collected.The maximum,minimum and the mean values of cumulative effective doses were 153.3,16.48 mSv and(52.3 ± 26.6) mSv.Conclusions Multiple trauma patients have high cumulative radiation exposure.Therefore,the management of cumulative radiation doses should be enhanced.To establish the individualized radiation exposure archives will be helpful for the clinicians and technicians to make decision wheather to image again and how to select the imaging parameters.

Objective To investigate the expression of extracellular signal-regulated kinase 1/2(ERK1/2) and transforming growth factor ?1(TGF-?1) in heart of spontaneous hypertensive rat.Methods The expression of ERK1/2 and TGF-?1 was determined by immunohistochemistry and Western blot.Results In arteriole of the heart of 8,16,and 20 week-old SHR,the rate of positive ERK1/2 staining at the endothelium(15.38%,76.97% and 72.72%,respectively) and at the vascular smooth muscle cell(VSMC) of 16 and 20 week-old SHR(5.49% and 6.83%,respectively)were significantly higher than that of control group(P

Objective:To study the sleep conditions, the prevalence of insomnia and the treatment problems in Jinuo People.Methods: 126 Jinuo People were investigated with “questionnaire of sleep conditions" which was designed for this study. Results: Sleep duration of Jinuo people decrease as they get old. The rate of siesta in people under 30-year-old was significantly higher than that of over 30-year-old. Among 126 Jinuo people,43 suffered from insomnia (34.12%). Psychiatric diseases(41.86%)and alcohol dependence(30.23%) were the two most common causes of insomnia. Among the patients with insomnia, 11(25.58%) sought for treatment. Conclusion: Compared with the general population, the prevalence of insomnia in Jinuo people was relatively higher. In view of the sampling bias and small sample, this results need to be validated by further study.

Objective To identify the factors related to the influence of medical research papers. Methods The influence of 1146 research papers by the hospital in the period 1996-2003 and its relationship with such factors as the backgrounds of the authors and the quality of the papers were studied u-sing regression analysis. Results Nine factors, including the impact factor, the status of the paper (being or not being one based on fund-supported research or representing research achievements), and the status of the author (being or not being chosen for talent cultivation and undertaking or not undertaking research projects), were significantly correlated with the influence of the papers. Conclusion In order to enhance the influence of medical research papers, it is necessary for research' management departments not only to encourage research personnel to publish papers in journals with high impact factors but also to step up efforts to better personnel cultivation, degree education, and project and achievement management so as to set up a quality and distinguished research contingent.

Objective To apply the IQM quality data of the GEM 3000 blood gas analyzer to evaluate the instrument performance and to compared it with the traditional evaluation method .Methods The precision and accuracy were calculated by using the IQM data ,the external quality control substance was adopted to verify the main performance indexes of the blood gas analyzer .Results The precision of PH ,PCO2 and PO2 calculated by using the IQM data was 0 .02% ,2 .16% and 0 .63% ,which by the traditional e-valuation method was 0 .07% ,1 .68% and 1 .28% respectively ,the accuracy of PH ,PCO2 and PO2 calculated by using the IQM data was 0 .00% ,1 .15% and 0 .49% ,which by the traditional evaluation method was 0 .11% ,2 .91% and 1 .07% respectively .Conclu-sion The intelligent quality management can provide objective and reliable indicators for the performance evaluation of the blood gas analyzer ,and compared with the traditional performance evaluation scheme which is more simple and convenient to operate .

Objective To study the antagonism of taurine-zinc coordination compound (TZC) to the adverse effect of mercury (Hg) on the neurons in the cerebral cortex. Methods 32 male Wistar rats aged 21days were randomly divided into 4 groups, one control group (fed on distilled water), three experimental groups fed on HgCl2 (0.06 g/L), HgCl2+0.23 g/L TZC and HgCl2+0.46 g/L TZC respectively, treated for one month. The NOS activity in the cerebral cortex neurons was determined by NADPH-d histochemistry. Results NADPH-d positive neurons increased in HgCl2 group (P

OBJECTIVE To study the mechanisms of imipenem resistance in Pseudomonas aeruginosa.METHODS PCR method was used to detect and analyze P.aeruginosa imipenem-resistance associated IMP gene and VIM gene of metallo-?-lactamases and outer membrane protein D2(OprD_2) gene.RESULTS All 35(imipenem)-resistant P.aeruginosa strains were negative for IMP gene and VIM gene of metallo-?-lactamases;and for OprD_2 gene;but 23S rRNA gene of all 35 P.aeruginosa strains was positive.CONCLUSIONS The study suggested that in(Xiangfan) area,Hubei Province P.aeruginosa doesn′t produce metallo-?-(lactamases),but in genetics it is(identified) that the loss of outermembrane protein D2(OprD_2) gene is the(essential) mechanisms of imipenem(resistance) in P.aeruginosa in Xiangfan area,Hubei Province.

Objective To study the relationship of different histological types and degrees of infiltration of gastric adenocarcinoma on lymph node metastasis.Methods The clinical data of 1 615 cases of gastric adenocarcinoma were analyzed retrospectively,and the rate and number of lymph node metastasis in different histological types and with different depths of invasion were statistically analyzed.Results There were 46 227 lymph nodes collected from 1615 cases,with an average of 28.62 per case. Lymph node metastasis was found in 1046(64.77%) cases of gastric cancer and 10 173(22.01%) lymph nodes. Different TNM stages showed different rates of metastasis: T1(M)2.78%,T1(SM)13.61%,T2 55.59%,and T3 84.84% respectively,while the metastatic rate for histolgically well-differentiated carcinoma was 50.25%,and for low-differentiated earcinoma was 78.95%,and both had statistically significant difference(P

Objective To explore the effects of H.pylori and crude extracted proteins secreted by H.pylori(broth culture filtrate protein,BCF-P)on acid secretion from isolated rabbit parietal cells.Methods Parietal cells from rabbit gastric mucosa were isolated and enriched with digestion and elutriation.H.pylori(NCTC 11637,CagA+ VacA+)were grown in liquid broth culture and BCF-P was precipitated with ammonium sulfate.The vacuolation activity of BCF-P was evaluated with neutral red dye uptake test in HeLa cell.Isolated parietal cells were incubated with H.pylori(bacteria/cell=100∶1)for 2 h and 16 h,or BCF-P(100μg/ml)for 1 h and 12 h.Acid secretion from parietal cells was studied using 14C-aminopyrine(14C-AP)accumulation indirectly and H+-K+ ATPase α subunit mRNA expression was assessed using RT-PCR.Results (1)BCF-P containing vacuolating cytotoxin(VacA)with vacuolation activity on HeLa cells had positive result on neutral red uptake test.(2)The basal expression of H+-K+ ATPase α subunit mRNA could be detected in isolated parietal cells and 14C-AP accumulation was significantly increased in response to the stimulation of histamine with different concentrations for 30 min(P＜0.05).These results indicated that the isolated parietal cells retain relative intact acid secretion function.(3)The histamine(1.0×104 mol/L)stimulated acid secretion was inhibited sustainedly in response to H.pylori by 81% at 2 h and by 94% at 16 h(P＜0.05).However,H+-K+ ATPase α subunit mRNA expression was up-regulated in tlle acute period(2 h)and was down-regulated in the chronic period (16 h)by H.pylori(P＜0.05).(4)BCF-P significantly inhibited the histamine-stimulated acid secretion by 24% at 1 h and by 58% at 12 h(P＜0.05),and this inhibition was accompanied by the down-regulated expression of H+-K+ATPase α subunit mRNA.Conclusions Intact H.pylori and VacA secreted by H.pylori could directly inhibit histamine-stimulated acid secretion from parietal cells and this inhibition may be mediated by the down-regulated H+-K+ ATPase expression.