Objective To evaluate the clinical therapeutic effectiveness of Ilizarov technique for the treatment of congenital brachymetatarsia.Methods We retrospectively analyzed the 5 patients who had been treated in our department for congenital brachymetatarsia (12 metatarsal bones in 10 feet) from January 2008 to January 2011.All of them were female,aged from 22 to 26 years (mean,24.2 years).All cases were bilaterally involved.Three of them suffered from bilateral brachymetatarsia of the first metatarsal bone,one from bilateral brachymetatarsia of the fourth metatarsal bone,and the other one from bilateral brachymetatarsia of the first and fourth metatarsal bones.Bone transport was conducted with a monolateral mini-fixator in 3 patients and with a modified semi-ring Ilizarov fixator in the other 2.Dorsal incision was used for all osteotomy before the external fixator was mounted.Bone transport began 7 days after surgery at a rate of 0.3 mm/d and was completed in 3 times.Results The 5 patients were followed up from 12 to 48 months (mean,22.0 months).The metatarsal bones were lengthened by 13.5 mm(12 to 17 mm) on average.The functions of the toe and ankle joint were normal without skin necrosis,vascular or nerve injury,tylosis of the footplate,or walking pain.The lengthened segments were mineralized well,with an average lengthening index of 48 d/cm(45 to 53 d/cm) and average time of external fixation of 64.8 days.At the last follow-up,the average AOFAS score was 92.2 points (90 to 95 points).Conclusions Although the Ilizarov technique necessitates a patient's prolonged fixation with an external fixator in the treatment of congenital brachymetatarsia,it has advantages of simplicity,minimal invasion,and satisfactory correction of brachymetatarsia.Consequently it is a good treatment for this disease.

BACKGROUND:The microenvironment of injured peripheral nerve was one of the most important factors that influence peripheral nerve regeneration. A favorable microenvironment for nerve regeneration would protect damaged neurons and promote axonal regeneration. OBJECTIVE:To simulate the microenvironment of peripheral neural regeneration with an amnion tube filled with polyglycolic polylactic acid(PLGA) filaments and autologous nerve tissue pulps and wrapped with a pedicled epimysium flap,and to study the feasibility to repair sciatic nerve defect. DESIGN,TIME AND SETTING:A randomized controlled study was performed in Experiment Animal Center of Guangdong Medical College from June 2006 to October 2007. MATERIALS:Thirty SD rats of clean grade and aged 2 months old were randomly divided into experimental group,control group,and standard group,with 10 rats in each group. The right side was operated,and the left side was control. Amnion was obtained from healthy,full-term,and spontaneous delivery fetus whose mothers provided the informed consent to establish amnion matrix membrane. Vicryl thread and amnion matrix membrane were used to make PLGA microfilament bridging graft. METHODS:The 6.0-mm sciatic nerve defect was bridged with different grafts,respectively:the amnion tube filled with Vicryl filaments and autologous nerve tissue pulps,and wrapped with a pedicled epimysium flap(experimental group) ;the amnion tube filled with autologous nerve tissue pulps(control group) ;autologous nerve(standard group) . MAIN OUTCOME MEASURES:Gross observation,histological examination,wet weight of the anterior tibial muscle,pass-through rate of myelinated nerve fibres and neural electrophysiology were assessed 8 and 12 weeks after the operation. RESULTS:At 12 weeks after operation,amyotrophy was lightly recovered in the experimental and standard groups except control group. Anterior tibial muscle at injured side was ruddy,well-stacked,and flexible in the experimental and standard groups,but the muscle was dark and poor elasticity. At 8 and 12 weeks after operation,there were significant differences in recovery rate of anterior tibial muscle between the three groups;in addition,at 12 weeks after operation,there were also significant differences in numbers of myelinated nerve fibers,cross-section area,vascular numbers of neural graft,vascular cross-section area,and compound muscle action potential amplitude of triceps surae between the three groups(P

Objective A study was performed to better define the teaching outcome of computers in teaching medical imaging. Methods 150 4rd-year students were randomly assigned to four groups and experimental group exposed to 20 radiology cases as well as to a voluntary weekly radiology lecture. Group A used computer-based cases with interactive elements; group B used computer-based cases without interactive elements; group C used paper-based cases with interactive elements; and group D was not exposed to the cases and served as a control group. Results On a multiple-choice question test,groups A,B,and C showed significant improvemen(t+10.6%,+15.7%,+13.5%,respectively),whereas group D did not(+2.7%). On an image interpretation test,group A and group B showed the most improvement (+15.8%,P

Objective To explore the feasibility and clinical resuhs of the tension skin flap of the pedicle of distally based skin flap or reverse-flow island flap. Methods Nine cases were repaired using reversed island flap pedicled with cutaneous branches of medial supramalleolar of posterior tibial artery in clinic,2 cases using distally based skin flap pedicled with the medial perforator of the posterior tibial artery,8 cases using reversalisland flap of peroneal artery lateral mulleolus skin perfarator 4 cases using reversed island flap pedicled with superficial peroneal nerve and its nutrient vessels. 3 cases using distally based sural neurocutaneous flap,and 2 cases using reversed island flap pedicled with dorsal fascia of hand.The area of the tension flap was 1.0 cm×1.0 cm to 5.0 am×3.5 cm. Results All the flaps completely survived.On the postoperative years follow-up,the texture of the flap was excellent,and primary healing was obtained at the donor site. Conclusion It's effective to design at the pedicle of the distally based flap or reversed island flap a tension skin flap,which can prevent the vessels from compressing or destroying.

Objective: To investigate the effects of selenium enriched probiotics on antioxidative activities and immune functions in weanling piglets. Methods: Twenty-four healthy weanling piglets (Duroc?Landrace?Yorkshire ) were divided into 3 groups. One served as control group (C) and two were supplemented different selenium (Se) sources namely selenium enriched probiotics (T1) and Na selenite (T2) as test groups respectively . The experiment lasted for 60 days. The blood GSH-Px activity, blood Se concentration, serum superoxide dismutase (SOD) activity, serum malonaldehyde (MDA) level, serum antibody level of swine fever, and the tissue content of selenium were determined. Results: Blood GSH-Px , Se concentration and serum SOD activity of T1, T2 groups were higher than those of control group after 60 d Se supplementation, and serum MDA content was markedly lower than control group. At the same time, the serum antibody level of swine fever, the tissue content of selenium in T1, T2 group were much higher than control group, and T1 group was higher than T2 group. Conclusion: Organic Se supplementation could significantly enhance immune function and antioxidative activities of weanling piglets.

Objective: To investigate the effects of selenium-enriched probiotics on immunity and antioxidation function in mice. Method: One hundred healthy KM mice, female and male in half, were randomly divided into control, sodium selenite, selenium-enriched yeast (Se yeast), selenium-enriched probiotics (Se probiotics) and probiotics groups. One milliliter of water, sodium selenite (2 ?g Se/ml), Se yeast (2 ?g Se/ml), Se probiotics (2 ?g Se/ml) or probiotics were respectively supplemented to five groups in oral (ig) every day. Whole experiment lasted for 28 d. During the experiment, immunity and antioxidation functions were measured respectively. Results: The activation of peritoneal macrophage and thymus index in Se probiotics group were significantly higher than those of other four groups. The spleen index of Se probiotics group was higher than that of control, sodium selenite and probiotics group. The spleen lymphocyte transformation rate in Se probiotics group was significantly or very significantly higher than that in control or probiotics group. In addition, blood GSH-Px and plasma SOD activity in Se probiotics group were significantly higher than those in control and sodium selenite group. Plasma MDA concentration in Se probiotics group was very significantly lower than that in control and sodium selenite group. Conclusion: Selenium-enriched probiotics supplementation could significantly enhance immunity and antioxidation function in mice.

Objective:To investigate the existence of side population cells with the potency of stem cells in human gallbladder carcinoma cell line GBC-SD and the differences in ABCG2,Oct-4 and CD34 expression among SP cells,non-SP cells and GBC-SD cells.Methods:SP and non-SP cells were sorted from GBC-SD cells by fluorescence-activated cell sorting(FACS).The expression of ABCG2,Oct-4 and CD34 in SP cells,non-SP cells,and GBC-SD cells was detected by reverse transcription-polymerase chain reaction(RT-PCR),Western blot,flow cytometry(FCM)and immunofluorescence chemistry.Results:SP cells with stem cell potency were isolated from GBC-SD cells with a proportion of(0.64±0.08)%.The metastatic ability of SP cells was obviously higher than that of non-SP cells and GBC-SD cells(P<0.05).The expression of ABCG2 was significantly higher in SP cells than in non-SP cells and GBC-SD cells[(89.56±3.86)%vs.(1.32±0.49)%and(12.37±1.61)%,P=0.001].The expression of Oct-4 in these cells was(94.87±1.40)%,(88.16±2.34)%and(90.17±1.61)%,respectively(P>0.05).CD34 was neady absent in these cells on protein level[(1.78±0.51)%vs.(0.63±0.21)%and(0.96±0.381)%,P>0.05)],but it was highly expressed in non-SP cells and GBC-SD cells and absent in SP cells off mRNA leve;.Conclusion:SP calls which hava the potency of stem cells,exist in human gallbladder carcinoma GBC-SD cell line and have the phenotype of ABCG2+Oct-4+CD34-.

Objective To study the roles of extracellular signal-regulated kinase(ERK1/2) and its substrate ets-like gene 1(Elk-1) in essential hypertension.Methods A comparative investigation was carried out to observe the expression of extracellular signal-regulated kinase1/2(ERKl/2) and Elk-1 in vascular smooth muscle cell (VSMC)and endothelium of gastrointestinal arterioles by means of immunohistochemistry in hypertension and nonhypertension patients.Results In the hypertension patients group,the positive rate of phosphorylated-ERK 1/2 in vascular smooth muscle cell(VSMC) and endothelium in gastrointestinal small arteries(8.32%and 4.97%,respectively) was significantly higher than that in the non-hypertension patients group(P

Objective To investigate the compliance with endocrine therapy(ET) and assess the factors associated with treatment accuracy for breast cancer.Methods 379 patients with hormone receptor-positive breast cancer undergoing complete treatment from Jun.2006 to Jun.2008 in Jiangsu Cancer Hospital were followed up.Factors related to compliance were analyzed.Results Among the 322 (85.0％) patients successfully interviewed,15 (4.7％) patients did not receive ET,43 (13.4％) patients stopped taking drugs after discharge,14 (4.3％)patients had intermittent ET,and the rest 250 patients obeyed 5-year oral ET regularly.The treatment accuracy was 77.6％ (250/332).We found that majority of withdraw occurred within 2 months and within 2 years,accounting for 39.5％ and 48.8％,respectively.Job and education status were relevant to these patients.Moreover,too much concern of adverse drug effects and difficulty of long-term medication were the main reasons to noncompliance.Conclusions ET is effective in hormone receptor-positive breast cancer patients and has been used as a conventional therapy.However,due to the long therapeutic period and lack of medical supervision after discharge,its treatment accuracy and compliances are becoming low,resulting in decreased efficiency.It is therefore necessary to investigate such patients' management to improve the compliance and treatment accuracy of ET.

Objective: To investigate the antitumor activities and apoptosis mechanisms of 3,7-dinitrodibenzobromonium salt (cBr) on human chronic myelogenous leukemia K562 cell line in vitro. Methods: The antitumor activities of cbr were tested by Trypanblau exclusion test, MTF coloretric assay and clonal forming test, and the mechanisms were studied by morphological analysis (microscopy, fluoreseencemicroscopy, electron microscopy) and flow cytometry analysis. Resuits: cBr could inhibit of the proliferation K562 cells and kill them significantly. The effects varied with different time and doses, and IC50 of cell colony forming rate was 0. 497 mmoL/L. Apoptosis rate tested by flow cytometry was 70.34%,and apoptotic characteristics could be found by microscopy and electron microscopy. Conclusion: cBr could inhibit K562 cell proliferation greatly, and could induce K562 cells into apoptosis.