OBJECTIVE:To observe the haemostasis outcome of treating radical gastrectomy for gastric cancer with Rep-tilase.METHODS:A total of30patients underwent radical gastrectomy for gastric cancer were randomly divided into reptilase group and control group,the preoperative and postoperative blood clotting functional parameter changes of the2groups were observed and the volumes of intraoperative blood loss and blood infusion were recorded.RESULTS:There was no significant difference in the blood clotting functional parameters before and after operation in the reptilase group,the intraoperative blood loss volume and blood infusion volume in this group were all lower than in the control group(P

High intensity focused ultrasound(HIFU) is a technique that ultrasound energy is focused on tumor target to function the treatment through the biological effects including heating effect, cavitation effect and mechanical effect of body to caused cell coagulation necrosis in vivo. Ultrasound contrast agent is one of the most important applicational agent for the treatment of hepatocellular carcinoma(HCC). We discussed in this paper the application of ultrasound contrast agent in the diagnose and focusing of target before the treatment and the assesement of treatment of HCC. Considering the enhancement of cavitation effect, better acoustic environment that enhance biological efficacy of HIFU during the treatment of HCC, ultrasound contrast agent is drawing more attention as an HIFU enhancement agent.

Objective: To investigate the effects of selenium enriched probiotics on antioxidative activities and immune functions in weanling piglets. Methods: Twenty-four healthy weanling piglets (Duroc?Landrace?Yorkshire ) were divided into 3 groups. One served as control group (C) and two were supplemented different selenium (Se) sources namely selenium enriched probiotics (T1) and Na selenite (T2) as test groups respectively . The experiment lasted for 60 days. The blood GSH-Px activity, blood Se concentration, serum superoxide dismutase (SOD) activity, serum malonaldehyde (MDA) level, serum antibody level of swine fever, and the tissue content of selenium were determined. Results: Blood GSH-Px , Se concentration and serum SOD activity of T1, T2 groups were higher than those of control group after 60 d Se supplementation, and serum MDA content was markedly lower than control group. At the same time, the serum antibody level of swine fever, the tissue content of selenium in T1, T2 group were much higher than control group, and T1 group was higher than T2 group. Conclusion: Organic Se supplementation could significantly enhance immune function and antioxidative activities of weanling piglets.

[Objective]Supracondylar fracture of the humerus is the most common fracture around elbow in children.In recent years,with the progress of minimally invasive technique,closed reduction and percutaneous Kirschner wire fixation has been widely applied in clinics to reduce the complications and surgical trauma.The relative literature concerning supracondylar fracture of the humerus in children was reviewed to find out the latest progress in classification criteria,the manipulation and reference standard of closed reduction,the configuration and effects of different percutaneous Kirschner wire fixation,and the complications and prevention measures for closed reduction and percutaneous pinning fixation.

Transnasal endoscopical biliary catheterization was done successfully in 44 cases,23 cases with complete data were reported.These cases consisted of decompression and drainage of benign and malignant biliary obstruction (12 cases),dissolution and irrigation treatment of remnants of ascaris in the biliary tract (5 cases),dissolution of gallstone (2 cases),irrigation for biliary calculi (2 cases),hemos-tasis after endoscopic sphincterotomy (EST,1 case),and the examination and culture of the bile ( 1 case).The results were satisfactory without complication.Problems concerning EST was discussed,and endoscopic retrograde biliary drainage (ERBD) and percutaneous transhepatic biliary drainage (PTBD) were compared.

This infant cereal chiefly consisted of rice meal, fullfat milk powder, rice germ and dried yeast, and fortified with ferrous lactate and calcium carbonate. This mixture contained 14.1% protein, 8.6% fat, 64.3% carbohydrate, 750mg% calcium, 10mg% iron, 1.0mg% zinc and proper amount of various kinds of vitamins.1. Growth test; 84 weaning SD rats were randomly divided into 6 groups:(A) Infant cereal group;(B) Control I; (C) Control II; (A') Infant cereal group; (B') Control I; (D) Casein group. The latter three groups contained 10% protein which were designed for calculating PER. All these different preparations were fed to 6 groups for 4 weeks. The results showed that the body weight gain, the FE, CE and PER were not significantly different between group A and B but there were significant difference between group A and C. The Hb was increased from 11.76g% ( ) and 11.46g%() to 14.30g% and 14.46g% respectively in group A. The bones developed well and no decalcification occured.2.Nitrogen balance test:(A). Infant cereal group; (B) Control I. The results indicated that the TD, BV and NPU of group A were 98.08%, 85.73% and 84.09% and of group B were 97.81%, 87.51% and 85.59% respectively. Both groups obtained the results of positive nitrogen balance, no significant statistical difference. The contents of hepatic and body nitrogen of two groups were also not significantly different.These data demonstrate that this infant cereal is able to promote the growth of weanling rats, to retain positive body nitrogen and to prevent the occurrence of anemia and rickets.

Objective: To investigate the effects of selenium-enriched probiotics on immunity and antioxidation function in mice. Method: One hundred healthy KM mice, female and male in half, were randomly divided into control, sodium selenite, selenium-enriched yeast (Se yeast), selenium-enriched probiotics (Se probiotics) and probiotics groups. One milliliter of water, sodium selenite (2 ?g Se/ml), Se yeast (2 ?g Se/ml), Se probiotics (2 ?g Se/ml) or probiotics were respectively supplemented to five groups in oral (ig) every day. Whole experiment lasted for 28 d. During the experiment, immunity and antioxidation functions were measured respectively. Results: The activation of peritoneal macrophage and thymus index in Se probiotics group were significantly higher than those of other four groups. The spleen index of Se probiotics group was higher than that of control, sodium selenite and probiotics group. The spleen lymphocyte transformation rate in Se probiotics group was significantly or very significantly higher than that in control or probiotics group. In addition, blood GSH-Px and plasma SOD activity in Se probiotics group were significantly higher than those in control and sodium selenite group. Plasma MDA concentration in Se probiotics group was very significantly lower than that in control and sodium selenite group. Conclusion: Selenium-enriched probiotics supplementation could significantly enhance immunity and antioxidation function in mice.

In the treatment of 126 cases of cervical spondylopathy by electroacupuncture on Jiaji (Ex-B 2),Jianyu (LI 15), Quchi (LI 11) and Hegu (LI 4), in combination with Tuina therapy, the results showed cure in 74 cases, improvement in 49 cases, failure in 3 cases and the total effective rate in 97.6%, after four courses of the treatment.

Objective To introduce the methods and key points for the external fixation combinel with limited percutaneous internal fixation plus colostomy in the treatment of open pelvic fracture concomitant with perineal laceration.Methods Nine patients with open pelvic fractures concomitant with perineallaceration were treated by external fixator combined with limited percutaneous internal fixer plus colostomy. Data including injury details, management and outcomes were collected for comparison.Results All the patients survived and attained bony union except for two patients with local infection that was healed several days after repeated wound debridement and use of wide spectrum antibiotics. All the patients had good extremity function at the latest follow-up. Conclusion External fixation combined with limited percutaneous internal fixation plus colostomy is a reliable, safe and less invasive procedure for the treatment of open pelvic fractures concomitant with perineal open wound.

Trehalose, a compatible solute, is widely used in food, cosmetics, pharmaceutical products and organ transplantation. Nowadays, trehalose is mostly produced by enzymatic synthesis with many secondary products and lowpurity. In this study, high amount of trehalose was produced by recombinant E. ccli fermentation. First, a bifunctional trehalose gene TPSP was amplified from genome of C. hutchinscoii. Second, an expression vector pTac-HisA containing TPSP was constructed and transformed into the host E. coli. Expression of this bifunctional enzyme-TPSP converted glucose to trehalose. The result suggested that TPSP from C. hutchinsonji has been successfully expressed in E. ccoi. High amount of extracellular trehalose generated from glucose by whole-cell catalysis and After optimization, the production of trehalose in shake flasks was improved to 1.2 g/L and the relative conversion rate reached 21%. The production in bioreactor reached 13.3 g/L and the relative conversion rate reached 48.6%. It is the first time to realize the functional expression of the bifunctional enzyme-TPSP of C. hutchinsonii in E. coli and achieved the conversion form glucose to trehalose. This study laid a foundation for industrial large-scale production of trehalose.
Bioreactors ; Catalysis ; Escherichia coli ; genetics ; Glucose ; Glucosyltransferases ; Industrial Microbiology ; Organisms, Genetically Modified ; Trehalose ; biosynthesis