Objective To investigate the application of multi-tumor markers protein chip diagnostic system in the diagnosis of gastric cancer.Methods Of the 80 patients with gastric cancer,70 patients who suffered from benign digestive disease and 50 healthy persons served as controls.The contents of alpha fetoprotein(AFP),carcinoembryonic antigen(CEA),neuron-specific enolase(NSE),carbohydrate antigen 125(CA125),carbohydrate antigen 153(CA153),carbohydrate antigen 242(CA242),carbohydrate antigen 199(CA199),prostate specific antigen(PSA),free prostate specific antigen(fPSA),ferritin(FER),?-human chorionic gonadotrophin(?-HCG) and human growth hormone(HGH) in the serum of 80 patients with gastric cancer,70 patients with benign digestive disease and 50 healthy persons were determined with multi-tumor markers protein chip assistance diagnostic system and analyzed with statistic method.Results There were 74 patients(92.75%) with tumor makers in serum in 80 cases of gastric cancer,10 cases(14.28%) with tumor makers in 70 cases of benign digestive disease,and 1 case with tumor makers in 50 cases of normal controls.The NSE,HGH,PSA and f-PSA were detected in the serum of partial patients with gastric cancer.Conclusion The application of multi-tumor markers protein chip diagnostic system has certain clinical value in the diagnosis of gastric cancer before surgery.

Objective:To investigate relationships between serum chemerin and bone mineral density (BMD) in patients with newly diagnosed Graves disease (GD).Methods:A total of 120 newly diagnosed GD patients with a course more than 3 months were enrolled from the Department of Endocrinology between June 2013 and June 2015.Sixty age-and sex-matched healthy people served as a normal control.Serum levels of chemerin,β-crosslaps (β-CTX),and N-MID-osteocalcin (N-MID-OT) were measured by ELISA.Fat mass and BMD were evaluated by dual energy X-ray absorptiometry (DEXA).Results:Compared with the normal control,the fat mass,lean weight,fat mass index (FMI) and body mass index (BMI) in the GD group were decreased,and BMD in all skeletal sites was decreased.There was a positive correlation between them (all P<0.05).Serum level of chemerin was increased and it was positively correlated with β-CTX or N-MID-OT level and negatively correlated with fat mass,FMI or BMI in the GD group.There was a negative correlation between chemerin level and BMD in femoral neck,total hip,lumbar or right forearm distal 1/3 (rs=-0.352,-0.279,-0.379,-0.289,-0.394;P<0.05).After adjusting for age,fat mass or BMI,the correlation of chemerin with total hip or bone mineral density remained significant (rs=-0.273,-0.378;P<0.05).Multiple linear regression analysis revealed that chemerin or BMI was correlated with BMD (P<0.05).Conclusion:The decrease of bone mineral density in patients with GD is not only related to the direct or indirect effect of excessive thyroid hormones on systemic and osteoblastic cells,but it is also related to the negative regulation of bone metabolism due to the elevated chemerin level.

For uncontrolled hemorrhagic shock the traditional method and target of resuscitation may be harmful. The existing data supported a strategy of less aggressive interventions to those shock with uncontrolled hemorrhage until a decisive surgery begin. The choice of liquor for resuscitation remains in controversy. A combined application of crystalloid and colloid liquor seems to be reasonable and may be accepted by most physicians. It should be noted that the new resuscitation strategy may not be suitable to some kinds of trauma, such as traumatic brain injury, and its long-term effects on to the prognosis should be further evaluated.

Objective To investigate the potential effect of the redox state of plasma factor Ⅺ (FXI) on the pathogenesis of elderly diabetic hypercoagulability and macroangiopathy.Methods The plasma levels of reduced FXI were detected in elderly type 2 diabetic(T2DM)patients with/without macroangiopathy (T2DM group/DMAP group) and healthy subjects (control group),and variables associated with reduced FXI were analyzed.Results Elderly patients with T2DM had higher plasma levels of reduced FXI as compared with healthy controls.The level of reduced FXI was significantly higher in patients with macroangiopathy than without macroangiopathy [control group:(80.6± 15.6) ％,T2DM group:(94.7 ± 16.0) ％,DMAP group (142.6 ± 36.5) ％,all P＜ 0.05].The multiple stepwise regression analysis showed that plasma levels of triglyceride,cholesterol and HDL-cholesterol were the independent predictors for reduced FXI.Conclusions The plasma level of reduced FXI is increased in elderly T2DM patients with macroangiopathy.The abnormality of lipid profiles may associate with the increment of reduced FXI.These findings maybe provide the novel mechanisms for diabetic hypercoagulability and macroangiopathy.

Objective To investigate the promoter methylation status and the transcription level of BLU in bladder carcinoma and their significance.Methods The methylation status of promoter and the transcription level of BLU were examined by methylation specific PCR(MSP) and RT-PCR in bladder cancer tissues from 54 patients,adjacent normal tissues from 45 cases and normal bladder tissues from 3 noncancerous patients.Results ① 31.5%(17/54) bladder cancer tissues showed BLU hypermethylation;neither the paired adjacent normal tissues nor the three normal bladder tissues showed BLU hypermethylation.② There was no significant correlation between the methylation status and the clinical pathological parameters in bladder cancer.③ The expression of BLU was absent in 42.2%(19/45) of bladder carcinoma from cystectomy.④ Loss of BLU expression was associated with the stage and grade of the tumor(P

OBJECTIVE To investigate the clinical degree of satisfaction to sterilizing-supplying center,and to consummate the development of the transition of its decentralized administration to centralized pattern.METHODS We designated the questionnaire of degree of satisfaction(DOS) and carried out the investigation.RESULTS The initiated DOS was 90.6%,the DOS in mid-stage(2006-2007) was 97.3%,and the DOS in the latest stage(2008) was 99.1%.CONCLUSIONS Centralized administration is effective pattern for the development of sterilizing-supplying center and important to ensure the quality of sterile materials and service for clinical medicine.

Objective To investigate the prevalence of the genotypes of extended spectrum ? lactamses (ESBLs) in klebsiella pneumoniae and eschrichia coli, which were isolated in Hangzhou City from 2000 to 2001. Methods Total of 198 isolates of E. coil and K. pneumoniae were isolated from five hospitals in Hangzhou. ESBLs-producing isolates were detected by inhibitor-potentiated disc diffusion test, Agar dilution method was used to determine the minimum inhibitory concentrations (MIC) of cefotaxime and ceftazidime.ESBLs genes were analysed by isoelectric focusing (IEF) assay and PCR initially,genotypes were determined by sequencing. Results 51 isolates (25.76%) from 198 E. coil and K. pneumoniae strains were obtained. In ESBLs producers, the ESBLs types of SHV, TEM, CTX-M were 17.65%,84.31% and 80.39% respectively. genotypes were: SHV 11,12;TEM 1;CTX M 3and CTX M 3 like, CTX M 13,14. respectively. Conclusions CTX M type were the major genotypes of ESBLs in klebsiella pneumoniae and eschrichia coli isolated in Hangzhou city.

The nutritional survey was made in 184 anorexia children aged 3-7, 123 of them were treated with various measures for three months. The results indicated that the children with anorexia had low intake and deficiency in energy, protein, minerals and vitamins. Most of them had zinc deficiency, but they simultaneously had two to three nutrients deficiency.The zinc treatment could improve most patient's syndrome and nutritional status. The effect of zinc treatment with vitamines and iron preparations was better than zinc treatment only. The effect of treatment with Chinese medicine also was able to cure the children with anorexia to certain extent.

Objective To investigate the methylation status of the promoter of RASSF1A gene in the tissues of bladder cancer and renal carcinoma and its role in tumorigenesis. Methods Methylation specific PCR (MSP) was used to examine the methylation status of RASSF1A in bladder cancer and renal carcinoma.The specimen included 45 cases of bladder cancer tissues and paired adjacent normal tissues from cystectomy,9 cases of bladder cancer tissues from microresection,3 cases of normal bladder tissues from people without tumor,12 cases of renal carcinoma tissues and paired adjacent normal tissues. Results 30 of 54(55.6%)bladder cancer tissues showed RASSF1A gene aberrant methylation.Of which,26 of 45 ( 57.8 %) bladder cancer tissues from cystectomy showed aberrant methylation,while one of the paired normal tissues showed gene methylation,4 of 9 (44.4%) bladder cancer tissues from microresection showed gene methylation.Eight of 12(66.7%)renal carcinoma tissues showed aberrant methylation,while none of the paired adjacent normal tissues showed gene methylation.None of the three normal bladder tissues showed aberrant methylation.There was no significant correlation between the methylation status and the clinical pathological parameters in bladder cancer tissues from cystectomy,though there was a trend that more frequent methylation was detected in higher stage bladder cancer. Conclusions Hypermethylation of RASSF1A gene in bladder cancer and renal carcinoma suggeste its possible role in the tumorigenesis of both tumors.

Objective To investigate the resistance of carbapenems,the production of metallobeta-lactamases(MBLs)and homology analysis of Psendomonas aeruginosa in China.Methods A total of 654 Pseudomonas aeruginosa isolates were collected from 28 hospitals in 16 difierent regions during the period from July 2006 to July 2007 as part of the Nosocomial Pathogen Resistance Surveillance(NPRS).MICs of imipenem and meropenem were determined by agar dilutiom PFGE was used to analyze the molecular epidemiology of carbapenem-resistant strains.MBLs were detected by Etest and PCR among carbapenem-non-sensitive strains.The transcription levels of MBLs were determined by real-time reverse transcriptase(RT)-PCR.Results Of the 645 Pseudomonas aeruginosa strains,245(37.5%)were resistant to imipenem and 183(28.0%)were resistant to meropenem.A total of 275 carbapenemnonsusceptible strains and 259 carbapenem-resistant strains were chosen for further study. Among the carbapenem-nonsusecptible strains,8.73%(24/275)of the strains carrried MBLs genes hy PCR,but only 6.55%(18/275)of the strains were detected as MBls-producers bv MBL Etest. The MBLs relative expression levels of the 6 MBL genotype-positive but phenotype-negative strains were significantly lower than those of the other 18 MBL genotype-and phenotype-positive strains(P＜0.05).The 259 carbapenemresistant Pseudomonas aeruginosa strains were divided into 89 clones by PFGE.The 24 MBL genotypepositive strains collected from 6 cities were divided into 9 clones by PFGE.Conclusions The carbapenems resistance of Pseudomonas aeruginosa is severe in China.Clonal dissemination of Pseudomonas aeruginosa has not been detected throughout China.MBLs are not the major carbapenems resistance mechanism in Pseudomonas aeruginosa.