The effects of the cyclin E expression levels on chemotherapeutic sensitivity of breast cancer cell line were explored. After the cyclin E expression was knockdown in MDA-MB-435 by RNA interference, FACS analysis and SA-beta-gal staining were used to evaluate the response sensitivity of breast cancer cells to DNA damage drugs (adriamycin, etc.). Adriamycin could induce G1 arrest in cyclin E knockdown MDA-MB-435 breast cell line and increase the percentage of cell senescence in cyclin E knockdown MDA-MB-435 cells. It was suggested that cyclin E knockdown could increase the chemotherapeutic sensitivity of breast cancer cells to DNA damage drugs.

Objective To study the relationship between the aged retirees' group's body mass index(BMI)distribution characteristics and relative cardiovascular diseases risk tactors.Methods One thousand three hundred and forty-three retirees in health medical examination were tested for systolic pressure, diastolic pressure,serum triglyceride,total cholesterol and fasting blood glucose.Groups with different BMI and different genders were tested for their mortality.Results The detective rates of BMI insufficiency,normal,overweight and obesity were 6.18%,51.67%,35.52%and 6.63%respectively.There was no statistic significance between different genders.The prevalence rates of hypertension,hyperlipidemia and diabetes mellitus(DM)were 46.09%,46.91%and 7.45%respectively.It showed that the prevalence rates of male hypertension and hyperlipidemia were significantly above those of the female's(P<0.05).As the BMI increased,hypertention,hyperlipidemia and DM rates increased too,and obviously increased in overweight group compared to normal BMI group(P<0.05).The detective rate of cardiovascular disease increased correspondingly with the increase of BMI.Conclusion BMI increasing is the important vascular relative risk factor for the aged retriee.

Objective To investigate the influence of LNG-IUS placement on endometrial thickness, recurrence rate and complications of patients with endometrial polyps and moderate anemia after TCRP. Methods 140 patients with endometrial polyps and moderate anemia were chosen from January 2014 to January 2016 and randomly divided into control group (70 patients) with TCRP used alone and observation group (70 patients) with LNG-IUS placement after TCRP; the endometrial thickness, hemoglobin levels and PBAC score before and after treatment, the recurrence rate and the complication incidence of both groups were compared. Results The endometrial thickness after treatment of observation group were significantly less than control group before the treatment (P < 0.05). The hemoglobin levels and PBAC score after treatment of observation group were significantly better than control group before the treatment (P < 0.05). The recurrence rate of observation group were significantly lower than control group (P < 0.05). There was no significant difference in the complication incidence between the two groups (P > 0.05). Conclusion LNG-IUS placement in treatment of patients with endometrial polyps and moderate anemia after TCRP can efficiently reduce the endometrial thickness, decrease the uterine bleeding amount, prevent the long-term recurrence and not increase the complications incidence.

BACKGROUND:Extracel ular vesicles (EVs) are a kind of subcel ular component produced by paracine mechanism including exosomes, microparticles and microvesicles, which have become hotspots in recent years. OBJECTIVE:To review the research status and progress of EVs, especial y in the studies about definition, secreting mechanism, isolation and identification, biological characteristics and functions in diseases as wel as in biomedical research. METHODS:The first author retrieved PubMed and CNKI databases for relative articles published from July 2006 to August 2016. The keywords were“extracel ular vesicles, exosome, microvesicle, microparticle”in English and Chinese, respectively.RESULTS AND CONCLUSION:A total of 44 eligible literatures are enrol ed. Almost al cel s can secrete EVs, which contain a variety of metrocyte-derived bioactive molecules, such as lipids, proteins, mRNAs, microRNA, lncRNA, cicrRNA, and non-coding RNA. These bioactive molecules are encapsulated in EVs or binding with the membrane. EVs are described to be involved in inflammation, immunity, signal transduction, cel survival and apoptosis, angiogenesis, thrombogenesis, and autophagy, which are of great significance to the maintenance of homeostasis and disease progression. Special EVs may be used as new biomarkers for the diagnosis and prognosis of many diseases and serve as novel tools in the fields of antitumor therapy, regenerative medicine, immunoregulation and vaccination and drug delivery. But the molecular mechanisms regulating the secretion of EVs and the specific pathways activated upon EVs interaction with the target cel are not ful y understood. Based on miRNA, lncRNA and circRNA are attracting researchers’ attention.

The spleno-and thyme-lymphocytes of mice were cultured in the media with various concentrations of zinc. The results showed that zinc acted as a weak mitogen and stimulating lymphocyte proliferation at 0.12-0.5? 10-4 mol/L. It also enhanced the lymphocyte proliferation to Con A and LPS. The optimal concentration was 0.12?10-4mol/L. It enhanced the MLR of spleno-lym-phocyte and increased the responsive ability of thymocyte to IL-1. Zinc was poisonous to cells at concentrations higher than 1.0 ?10-4mol/L.

Objective To measure circulating vascular endothelial growth factor-C(VEGF-C) levels in(patients) with colorectal carcinoma,and assess the clinical significance in diagnosis of lymph node metastasis.Methods Sixty-six patients with colorectal carcinoma and 30 healthy control patients were included in this study.Circulating VEGF-C and VEGF levels were assessed by enzyme-linked immunosorbent assay.Results Serum VEGF-C and VEGF concentration was higher in patients with colorectal carcinoma than in healthy control patients(P

Objective To investigate the effects of Na +-dependent high-affinity dicarboxylate cotransporter 3(NaDC3) and glucose on the transport of succinate in human proximal tubular epithelial cells (HKC) . Methods Western blot was used to detect the expression of hNaDC3 protein in the pcDNA3-hNaDC3, pcDNA3-AhNaDC3 and pcDNA3, transfected HKC, and normal HKC cell. Analysis of succinate contents in the intaking-buffer at the various time points and in the variouse concentrations of glucose were determined by capillary zone electrophoresis. Results The cell transfected with pcDNA3- hNaDC3 was found to have over-expression of hNaDC3 protein for about 1.5 times that of the normal HKC, while succinate content in intaking-buffer was decreased. Although the cells transfected with pcDNA3-AhNaDC3 showed lower expression of hNaDC3 protein, being about 0.6 times that of normal HKC cells, the decrease of succinate in intaking buffer was not obviously effected. There were no differences in hNaDC3 protein content and succinate intaking between the cells transfected with pcDNA3 and normal HKC cells. With the increase in glucose concentration, the decrease of succinate in intaking-buffer was accelerated. Conlusion The results suggested that the overexpression of hNaDC3 protein can cause the succinate-intake more quickly in HKC.

Objective To investigate the diagnostic value of determination of serum carcinoembryonic antigen (CEA) levels for primary liver carcinoma and metastatic liver cancer.Methods Serum CEA levels in 365 patients with liver cancer (273 with HCC and 92 with metastatic liver cancer accordingly) and 33 controls were determined with ELISA. Data were analysed with SPSS.Results Serum CEA levels were (7.52?18.04)?g/L in patients with liver cancer and (0.99?0.79)?g/L in controls. Taking 2.5 ?g/L as the cut-off level,the positive rate was 29.86% and 6.06% respectively. The values were significantly higher than those in controls (P

Objective To improve the identification of rush pneumoconiosis.Methods To analyze 47 cases of rush pneumoconiosis treated in our hospital from 2003 May to 2008 December.Results The male occupied 74% of 47 patients.The average age of onset was 34.2 ranging from 26.1 to 42.3.78.7% patiets presented with cough,sputum production,chest pain and dyspnea.4.2% patiets had PaO2 45 mmHg.85.1% patients' chest X-ray and CT images showed nodule shadows,interstitial fibrosis and ground glass attenuation.42.6% patients had restrictive ventilation disorders,2.1% obstrutive ventilation disorders 4.2% mixed ventilation disorders,and 74.5% decreased diffusing capacities.Through fiberoptic bronchoscopy,carbon sediment were seen on the 10% patients' bronchial walls,and bronchial lumen were distorted and stiff.Chronic inflammaion increased,macrophages and fibre tissues,and 21.2% dust cells were seen in pathology.All patients were treated according to their clinic symptoms.Conclusion Rush-mat dust was the main cause of rush pneumoconiosis,there were no special ways to cure this disease,prevention was the key to eliminate rush pneumoconiosis.

OBJECTIVE To investigate the expression and clinical significance of epidermal growth factor receptor (EGFR), the mutations of gene EGFR, KRAS and BRAF in sinonasal intestinal-type adenocarcinomas. METHODS We investigated the EGFR protein expression by immunohistochemistry method with antibodies targeting the extracellular domain, the intracellular domain, and the phosphorylated isoform in a series of 11sinonasal intestinal-type adenocarcinomas tissues. EGFR, KRAS, and BRAF mutational status were detected by DNA direct sequencing and Melt curve method.RESULTS The findings were analyzed with respect to clinical data, histological typing, and outcome of the patients. EGFR was expressed in 72.7% tumors with a focal distribution with both extracellular domain and intracellular domain, which showed a significantly correlation. p-EGFR was expressed in 9.1% tumors. 9.1% tumors had the mutation of EGFR gene in exon 19, 9.1% tumors had the mutation in exon 2 and exon 15 of gene KRAS and BRAF respectively. There was no association between these molecular features and the survival period of the patients. CONCLUSION The current study revealed various EGFR expression patterns in sinonasal intestinal-type adenocarcinomas, that indicated the tumor heterogeneity. Sinonasal intestinal-type adenocarcinomas share common alterations of the EGFR pathway as the mutation of EGFR, KRAS and BRAF genes, but with a lower frequency.